Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (17): 3646-3652.doi: 10.3864/j.issn.0578-1752.2012.17.023

• RESEARCH NOTES • Previous Articles     Next Articles

Differential Protein Analysis of Chinese Gooseberry Ovary After Fertilization by a 2D-DIGE Approach

 QI  Xiu-Juan, FANG  Jin-Bao, CHEN  Jin-Yong, GU  Hong, ZHANG  Shao-Ling   

  1. 1.南京农业大学梨工程技术研究中心，南京 210095
    2.中国农业科学院郑州果树研究所/果树生长发育与品质控制重点开放实验室，郑州 450009
  • Received:2012-03-01 Online:2012-09-01 Published:2012-05-29

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Abstract: 【Objective】The changes of Chinese gooseberry (Actinidia arguta) ovary proteins and morphology before and 120 h after pollination were analyzed to provide valuable evidence for further elucidating the molecular mechanism of double fertilization.【Method】Fluorescence paraffin technique,differential in-gel electrophoresis (DIGE), Matrix-assisted laser-desorption/ionization Time of ?ight/Time of ?ight (MALDI-TOF/TOF) and bioinformatic technology were used in the experiment.【Result】The embryo sac in the ovary completed its double fertilization 120 h after pollination. About 1 500 protein points were detected by DeCyder 6.5 (Amersham Bioscience), and 55 proteins were differentially expressed at ovary before and 120 h after pollination. Thirteen protein spots (differential ratio＞2.0) were identified with MALDI-TOF/TOF, of these proteins,8 belong to 6 kinds of proteins. Five of the six proteins are up-regulated while one is down-regulated.【Conclusion】According to the expression differences of the proteins at ovary before and after fertilization, several proteins identified might be related to the process of the double fertilization of Chinese gooseberry.

Key words: Chinese gooseberry, fertilization, ovary, proteome

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