Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 1972-1981.doi: 10.3864/j.issn.0578-1752.2012.10.010

• HORTICULTURE • Previous Articles     Next Articles

Cloning, Expression and Promoter Analysis of AP1 Homologous Gene from Strawberry (Fragaria×ananassa)

 ZOU  Dong-Mei, LIU  Yue-Xue, ZHANG  Zhi-Hong, LI  He, MA  Yue, DAI  Hong-Yan   

  1. 沈阳农业大学园艺学院，沈阳110866
  • Received:2011-11-04 Online:2012-05-15 Published:2012-03-22

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Abstract: 【Objective】The aim of this study was to isolate the AP1 homologous gene from strawberry (Fragaria×ananassa), analyze its expression level in different tissues, organs and at different growth stages of flowers and elucidate the role of it in the floral development progress of strawberry.【Method】The fragment of an AP1 homologous gene was amplified from young leaves and flower buds of strawberry with the degenerate primers designed according to the conserved sequences of AP1 homologous genes in the other species. The full-length cDNA sequence of this gene was obtained with RACE technique. Real time RT-PCR was used to analyze the expression patterns of this gene in different tissues, organs and at different growth stages of flowering of strawberry. A promoter sequence was isolated with chromosome walking methods.【Result】 An AP1 homologous gene in strawberry was cloned from cultivar ‘Huaji’ and it was designated as FaAP1. The CDS length of FaAP1 is 735 bp and encodes a predicted protein of 245 amino acid residues. Its amino acid sequence shows a highest homology with the AP1-1 of Rosa, up to 92%, and also has a homology of 64.00% when compared with the AtAP1 of Arabidopsis thaliana. FaAP1 is a MIKC type MADS-box gene for its amino acid sequence contained the typical domains of MADS-box, I-region, K-box and C-terminal. The results of real time RT-PCR showed that the expression patterns of FaAP1 changed in different tissues, organs and flowers of different growth stages. In addition to the TATA/CAAT-box, its promoter also contained some specific regulatory elements.【Conclusion】FaAP1, an AP1 homologous gene in strawberry, was isolated and it may play an important role in the progress of flowering in strawberry.

Key words: strawberry, AP1 homologue, isolation, expression, promoter

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