Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 1972-1981.doi: 10.3864/j.issn.0578-1752.2012.10.010

• HORTICULTURE • Previous Articles     Next Articles

Cloning, Expression and Promoter Analysis of AP1 Homologous Gene from Strawberry (Fragaria×ananassa)

 ZOU  Dong-Mei, LIU  Yue-Xue, ZHANG  Zhi-Hong, LI  He, MA  Yue, DAI  Hong-Yan   

  1. 沈阳农业大学园艺学院，沈阳110866
  • Received:2011-11-04 Online:2012-05-15 Published:2012-03-22

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Abstract: 【Objective】The aim of this study was to isolate the AP1 homologous gene from strawberry (Fragaria×ananassa), analyze its expression level in different tissues, organs and at different growth stages of flowers and elucidate the role of it in the floral development progress of strawberry.【Method】The fragment of an AP1 homologous gene was amplified from young leaves and flower buds of strawberry with the degenerate primers designed according to the conserved sequences of AP1 homologous genes in the other species. The full-length cDNA sequence of this gene was obtained with RACE technique. Real time RT-PCR was used to analyze the expression patterns of this gene in different tissues, organs and at different growth stages of flowering of strawberry. A promoter sequence was isolated with chromosome walking methods.【Result】 An AP1 homologous gene in strawberry was cloned from cultivar ‘Huaji’ and it was designated as FaAP1. The CDS length of FaAP1 is 735 bp and encodes a predicted protein of 245 amino acid residues. Its amino acid sequence shows a highest homology with the AP1-1 of Rosa, up to 92%, and also has a homology of 64.00% when compared with the AtAP1 of Arabidopsis thaliana. FaAP1 is a MIKC type MADS-box gene for its amino acid sequence contained the typical domains of MADS-box, I-region, K-box and C-terminal. The results of real time RT-PCR showed that the expression patterns of FaAP1 changed in different tissues, organs and flowers of different growth stages. In addition to the TATA/CAAT-box, its promoter also contained some specific regulatory elements.【Conclusion】FaAP1, an AP1 homologous gene in strawberry, was isolated and it may play an important role in the progress of flowering in strawberry.

Key words: strawberry, AP1 homologue, isolation, expression, promoter

[1]Taylor D R. The physiology of flowering in strawberry. Acta Horticulture, 2000, 567: 245-252.

[2]Huijser P, Klein J, Lonnig W E, Meijer H, Saedler H, Sommer H. Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS-box gene squamosa in Antirrhinum majus. The EMBO Journal, 1992, 11(4): 1239-1249.

[3]Mandel M A, Gustafson-Brown C, Savidge B, Yanofsky M F. Molecular characterizatio of the Arabidopsis ?oral homeotic gene APETALA1. Nature, 1992, 360: 273-277.

[4]Adrian J, Torti S, Turck F. From decision to commitment: the molecular memory of flowering. Molecular Plant, 2009, 4: 628-642.

[5]Searle I, He Y H, Turck F, Vincent C, Fornara F, Kröber S, Amasino R A, Coupland G. The transcription factor FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis. Genes & Development, 2006, 20(7): 898-912.

[6]Kim S, Koh J, Ma H, Hu Y, Endress P K, Hauser B A, Buzgo M, Soltis P S, Soltis D E. Sequence and expression studies of A-,B-, and E-class MADS-box homologues in Eupomatia (Eupomatiaceae): support for the bracteate origin of the calyptra. International Journal of Plant Sciences , 2005, 66: 185-198.

[7]Fornara F, Parenicova L, Falasca G, Pelucchi N, Masiero S, Ciannamea S, Lopez-Dee Z, Altamura M M, Colombo L, Kater M. Functional characterization of OsMADS18, a member of the AP1/SQUA subfamily of MADS box genes. Plant Physiology, 2004, 135: 2207-2219.

[8]Masiero S, Imbriano C, Ravasio F, Favaro R, Pelucchi N, Gorla M, Mantovani R, Colombo L, Kater M. Ternary complex formation between MADS-box transcription factors and the histone fold protein NF-YB. Journal of Biological Chemistry, 2002, 277: 26429-26435.

[9]肖  敏, 张志宏, 杨洪一, 代红艳, 李  贺. PCR检测草莓镶脉病毒的稳定性研究.果树学报, 2005, 22(5): 483-487.

Xiao M, Zhang Z H, Yang H Y, Dai H Y, Li H. Enhancing the stability of detection of Strawberry vein banding virus by PCR. Journal of Fruit Science, 2005, 22(5): 483-487. (in Chinese)

[10]李  贺, 印东升, 王志刚, 黄飞飞, 常琳琳, 张志宏. 草莓不同器官中microRNA表达差异研究.果树学报, 2009, 26(5): 632-637.

Li H, Yin D S, Wang Z G, Huang F F, Chang L L, Zhang Z H. Study on the difference in expression profiles of microRNA among different organs of strawberry. Journal of Fruit Science, 2009, 26(5): 632-637. (in Chinese)

[11]Litt A, Irish V F. Duplication and diversification in the APETALA1/ FRUITFULL floral homeotic gene lineage: implication for the evolution of floral development. Genetics, 2003, 165: 821-833.

[12]Becker A, Theiben G. The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Phylogenet Evolution, 2003, 29: 464-489.

[13]Kagale S, Links M G, Rozwadowski K. Genome-wide analysis of ethylene-responsive element binding factor-associated amphiphilic repression motif-containing transcriptional regulators in Arabidopsis. Plant Physiology, 2010, 152: 1109-1134.

[14]Berbel A, Navarro C, Ferrándiz C, Cañas AL, Madueño F, José-Pío Beltránet. Analysis of PEAM4, the pea AP1 functional homologue, supports a model for AP1-like genes controlling both ?oral meristem and ?oral organ identity in different plant species. Plant Journal, 2001, 25: 441-451.

[15]Münster T, Pahnke J, di Rosa A, Kim J T, Martin W, Saedler H, Theissen G. Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. Proceedings of the National Academy of Sciences of the USA, 1997, 94: 2415-2420.

[16]Nam J M, Kim J Y, Lee S Y, An G H, Ma H, Nei M. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proceedings of the National Academy of Sciences of the USA, 2004, 101: 1910-1915.

[17]Kaufmann K, Melzer R, Theissen G. MIKC-type MADS-domain proteins: structural modularity, protein interactions and network evolution in land plants. Genes, 2005, 347: 183-198.

[18]Gustafson-Brown C, Savidge B, Yanofsky M. Regulation of the Arabidopsis floral homeotic gene APETALA1. The Plant Cell, 1994, 76: 131-143.

[19]Hempel F, Weigel D, Mandel M, Mandel Mzambryski P, Feldman L, Yanofsky M. Floral determination and expression of floral reguhtory genes in Arabidopsia. Development, 1997, 124(19): 3845-3853.

[20]Chi Y J, Huang F, Liu H C, Yang S P, Yu D Y. An APETALA1-like gene of soybean regulates ?owering time and speci?es ?oral organs. Plant Physiology, 2011, 168: 2251-2259.

[21]Urao T, Yamaguchi-Shinozaki K, Urao S, Shinozaki K. An Arabidopsis MYB homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence. The Plant Cell, 1993, 5(11): 1529-1539.

[22]Hancock J F, Luby J J. Genetic resources at our doorstep: the wild strawberries. ProQuest Biology Journal, 1993, 43(3): 141-147.

[23]Folta K M, Davis T M. Strawberry genes and genomics. Critical Reviews Plant Science, 2006, 25: 399-415.

[24]Darrow G M. The Strawberry: History, Breeding and Physiology. Holt, Rinehart and Winston, New York, 1966.

[25]Shulaev V, Sargent D J, Crowhurst R N, Mockler T C, Folkerts O, Delcher A L, Jaiswal P, Mockaitis K, Liston A, Mane S P, Burns P, Davis T M, Slovin J P, Bassil N, Hellens R P, Evans C, Harkins T, Kodira C, Desany B, Crasta O R, Jensen R V,Allan A C, Michael T P, Setubal J C, Celton J M, Rees D J G., Williams K P, Holt S H, Ruiz Rojas J J, Chatterjee M, Liu B, Silva H, Meisel L, Adato A, Filichkin S A, Troggio M, Viola R, Ashman T L, Wang H, Dharmawardhana P, Elser J, Raja R, Priest H D, Bryant Jr D W, Fox S E, Givan S A, Wilhelm L J, Naithani S, Christoffels A, Salama D Y, Carter J, Lopez Girona E, Zdepski A, Wang W, Kerstetter R A, Schwab W, Korban S S, Davik J, Monfort A, Denoyes-Rothan B, Arus P, Mittler R, Flinn B, Aharoni A, Bennetzen J L, Salzberg S L, Dickerman A W, Velasco R, Borodovsky M, Veilleux R E, Folta K M. The genome of woodland strawberry (Fragaria vesca). Nature Genetics, 2011, 43: 109-116.

[26]Mouhu K, Hytönen T, Folta K, Rantanen M, Paulin L, Auvinen P, Elomaa P. Identification of flowering genes in strawberry, a perennial SD plant. BMC Plant Biology, 2009, 9: 122.
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