Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 1964-1971.doi: 10.3864/j.issn.0578-1752.2012.10.009

• HORTICULTURE • Previous Articles     Next Articles

Protective Effects of Exogenous Chitosan on Oxidative Damage in Pepper Seedling Leaves Under Water Stress

 MA  Yan-Xia, YU  Ji-Hua, ZHANG  Guo-Bin, CAO  Gang   

  1. 甘肃农业大学农学院，兰州 730070
  • Received:2011-06-29 Online:2012-05-15 Published:2012-03-27

PDF

931

Cited

13

Abstract: 【Objective】The aim of the experiment was to study the protective effects of exogenous chitosan on protective enzymes, antioxidant substances, osmotic adjustment material and membrane lipid peroxidation of pepper seedlings under water stress.【Method】The protective effects of exogenous chitosan at different concentrations(10, 30, 50, 80 mg•L-1) on oxidative damage in leaves of pepper seedlings under water stress were investigated with peppers varieties ‘Longjiao No.3’, ‘Longjiao No.2’ and ‘Zhongyan No.8’ as materials.【Result】Under water stress, the activities of superoxide dismutase and peroxidase, proline content, malondialdehyde content and relative conductivity of pepper seedlings were significantly higher than that of control (P<0.05), and the contents of glutathione and ascorbic acid were significantly lower than other treatments. The activities of superoxide dismutase and peroxidase, the relative conductivity, malondialdehyde and proline content apparently decreased, but the soluble proten contents and the contents of glutathione and ascorbic acid increased in treatments of different concentrations of chitosan compared with control.【Conclusion】Chitosan at 50 and 80 mg•L-1 increased the content of antioxidant substances, effectively prevented the accumulation of malondialdehyde and proline, mitigated the lipid peroxidation caused by water stress, and enhanced the plant's drought resistance.

Key words: chitosan, pepper, water stress, antioxidant enzymes, antioxidant substances, osmotic adjustment substance

[1]李  江, 汤红玲, 陈惠萍. 外源一氧化碳对干旱胁迫下水稻幼苗抗氧化系统的影响. 西北植物学报, 2010, 30(2): 330-335.

Li J, Tang H L, Chen H P. Effect of exogenous carbon monoxide on antioxidative system in rice seedlings under drought stress. Acta Botanica Boreali-Occidentalia Sinica, 2010, 30(2): 330-335. (in Chinese)

[2]付秋实, 李红岭, 崔  健, 赵  冰, 郭仰东. 水分胁迫对辣椒光合作用及相关生理特性的影响. 中国农业科学, 2009, 42(5): 1859-1866.

Fu Q S, Li H L, Cui J, Zhao B, Guo Y D. Effects of water stress on photosynthesis and associated physiological characters of Capsicum annuum L.. Scientia Agricultura Sinica, 2009, 42(5): 1859-1866. (in Chinese)

[3]钟  杨, 陈洪国. 芸苔素内酯水剂对辣椒幼苗水分胁迫生理的影响. 北方园艺, 2011(2):42-44.

Zhong Y, Chen H G. Effects of brassinolide treatment on pepper seedlings physiological under water stress. Northern Horticulture, 2011(2): 42-44. (in Chinese)

[4]马鹏鹏. 甲壳素及其衍生物在农业生产中的应用. 植物生理学通讯, 2001, 37(5): 475-478.

Ma P P. Applications of chitin and its derivatives in agriculture. Plant Physiology Communications, 2001, 37(5): 475-478. (in Chinese)

[5]邓雨艳, 明  建, 张昭其, 曾凯芳.壳聚糖诱导脐橙果实抗病性、水杨酸及活性氧代谢变化. 中国农业科学, 2010, 43(4): 812-820.

Deng Y Y, Ming J, Zhang Z Q, Zeng K F. Effect of chitosan on salicylic acid and active oxygen metabolism of navel orange fruit. Scientia Agricultura Sinica, 2010, 43(4): 812-820. (in Chinese)

[6]宋士清, 尚庆茂, 郭世荣, 张志刚. 壳聚糖对黄瓜幼苗抗盐的协同生理作用研究. 西北植物学报, 2006, 26(3): 435-441.

Song S Q, Shang Q M, Guo S R, Zhang Z G. Physiological synergisms of chitosan on salt resistance of cucumber seedlings. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(3): 435-441. (in Chinese)

[7]郁继华, 闫晓花, 张洁宝, 雍山玉. 壳聚糖对低温胁迫下辣椒幼苗活性氧产生和保护酶活性的影响.植物生理学通讯, 2007, 43(4): 725-726.

Yu J H, Yan X H, Zhang J B, Yong S Y. The effects of chitoson on active oxygen generation and protective enzyme activity in leaves of pepper seedlings under low temperature stress. Plant Physiology Communications, 2007, 43(4): 725-726. (in Chinese)

[8]王延峰, 郝振荣, 常海飞. 壳聚糖处理对苜蓿幼苗抗旱性的影响. 安徽农业科学, 2008, 36(24): 10322-10323.

Wang Y F, Hao Z R, Chang H F. Effects chitosan treatment on drought resistance of Alfalfa seedlings. Journal of Anhui Agricultural Sciences, 2008, 36(24): 10322-10323. (in Chinese)

[9]刘  婷, 赵永富. 低聚壳聚糖对小麦幼苗抗干旱胁迫的影响. 江苏农业科学, 2009 (1): 88-89.

Liu T, Zhao Y F. Effect of chitosan-oligosaccharide on seedling of wheat under drought stress. Jiangsu Agricultural Sciences, 2009 (1): 88-89. (in Chinese)

[10]顾丽嫱. 外源CTS对干旱胁迫下蝴蝶兰幼苗生理生化指标的影响. 西南农业学报, 2011, 24(1): 90-93.

Gu L Q. Effects of exogenous chitosan on physiological characteristics of phalaenopsis seedlings under draught stress. Southwest China Journal of Agricultural Sciences, 2011, 24(1):90-93. (in Chinese)

[11]郑炳松.现代植物生理生化研究技术. 北京: 气象出版社, 2006: 91-92, 41-42.

Zheng B S. Research Techniques in Contemporary Plant Physiology and Biochemistry. Beijing: China Meteorological Press, 2006: 91-92, 41-42. (in Chinese)

[12]李合生. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2000: 246-249.

Li H S. Experimental Principle and Technique for Plant Physiology and Biochemistry. Beijing: Higher Education Press, 2000: 246-249. (in Chinese)

[13]Ellman G L. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 1959, 82:70-77.

[14]赵世杰, 许长城, 邹  琦, 孟庆伟. 植物组织中丙二醛测定方法的改进. 植物生理学通讯, 1994, 30(3): 207-210.

Zhao S J, Xu C C, Zou Q, Meng Q W. Improvements of method for measurement of malondialdehyde in plant tissues. Plant Physiology Communications, 1994, 30(3): 207-210. (in Chinese)

[15]郝再彬, 苍  晶, 徐  仲. 植物生理实验.哈尔滨:哈尔滨工业大学出版社, 2004: 101-104.

Hao Z B, Cang J, Xu Z. Experimental Plant Physiology. Harbin: Harbin Institute of Technology Press, 2004: 101-104.(in Chinese)

[16]职明星, 李秀菊. 脯氨酸测定方法的改进. 植物生理学通讯, 2005, 41(3)：355-357.

Zhi M X, Li X J. Improvement on the method for measuring praline content. Plant Physiology Communications, 2005, 41(3): 355-357. (in Chinese)

[17]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bingding. Analytical Biochemistry, 1976, 72: 248-254.

[18]Valladares F, Wright S J, Lasso E, Kitajima K, W. Pearcy R. Plastic phenotypic response to light of 16 congeneric shrubs from a panamanian rainforest. Ecology, 2000, 81(7): 1925-1936.

[19]王  芳, 董  乐, 戴聪杰, 林  娈, 林  静. 杨梅Cu/Zn超氧化物歧化酶基因（MrSOD1）cDNA的克隆及表达分析. 中国农学通报, 2010, 26(22): 27-33.

Wang F, Dong L, Dai C J, Lin L, Lin J. Molecular cloning and expression analysis of Cu/Zn superoxide dismutase gene (MrSOD1) cDNA from Myrica rubra. Chinese Agricultural Science Bulletin, 2010, 26(22): 27-33. (in Chinese)

[20]李  明, 王根轩. 干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响. 生态学报, 2002, 22(4): 503-507.

Li M, Wang G X. Effect of drought stress on activities of cell defense enzymes and lipid peroxidation in Glycyrrhiza uralensis seedlings. Acta Ecologica Sinica. 2002, 22(4): 503-507. (in Chinese)

[21]张明生, 谈  锋, 谢  波, 张启堂, 付玉凡, 杨春贤, 杨永华. 甘薯膜脂过氧化作用和膜保护系统的变化与品种抗旱性的关系. 中国农业科学, 2003, 36(11): 1395-1398.

Zhang M S, Tan F, Xie B, Zhang Q T, Fu Y F, Yang C X, Yang Y H. Relationship of peroxidation of membrane-lipid and membrane protection system in sweetpotato under water stress with drought resistance. Scientia Agricultura Sinica, 2003, 36(11): 1395-1398. (in Chinese)

[22]朱  慧, 马瑞君, 吴双桃, 李  云, 张晓玲, 郑灿钟. 干旱胁迫对五爪金龙种子萌发与幼苗生长的影响. 西北植物学报, 2009, 29(2): 344-349.

Zhu H, Ma R J, Wu S T, Li Y, Zhang X L, Zheng C Z. Seed germination and seedling growth of invasive weed Ipomoea cairica under drough stress. Acta Botanica Boreali-Occidentalia Sinica, 2009, 29(2): 344-349(in Chinese)

[23]Tausz M, Sircelj H, Grill D. The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid. Journal of Experimental Botany, 2004, 55: 1955-1962.

[24]王贺正, 马  均, 李旭毅, 李  艳, 张荣萍, 汪仁全. 水分胁迫对水稻结实期活性氧产生和保护系统的影响. 中国农业科学, 2007, 40(7): 1379-1387.

Wang H Z, Ma J, Li X Y, Li Y, Zhang R P, Wang R Q. Effects of water stress on active oxygen generation and protection system in rice during grain filling stage. Scientia Agricultura Sinica, 2007, 40(7): 1379-1387. (in Chinese)

[25]杜金友, 陈晓阳, 胡东南, 高  琼, 陈  佳. 干旱胁迫条件下几种胡枝子渗透物质变化的研究. 华北农学报, 2004, 19(S1): 40-44.

Du J Y, Chen X Y, Hu D N, Gao Q, Chen J. Studies on change of osmolytes in lespedeza michxunder drought stress. Acta Agriculturae Boreali-Sinica, 2004, 19(S1): 40-44. (in Chinese)

[26]张明生, 彭忠华, 谢  波, 谈  锋, 张启堂, 付玉凡, 杨春贤, 杨永华. 甘薯离体叶片失水速率及渗透调节物质与品种抗旱性的关系. 中国农业科学, 2004, 37(1): 152-156.

Zhang M S, Peng Z H, Xie B, Tan F, Zhang Q T, Fu Y F, Yang C X, Yang Y H. Relationship between water loss rate of cutting leaves and osmotic regulators under water stress and drought resistance in sweet potato. Scientia Agricultura Sinica, 2004, 37(1): 152-156. (in Chinese)

[27]从心黎, 黄绵佳, 冯  妍, 杨意伯. 外源壳聚糖对干旱胁迫下红掌苗的生理效应. 植物生理学通讯, 2008, 44(1): 103-105.

Cong X L, Huang M J, Feng Y, Yang Y B. Physiological effects of exogenous chitosan on seedlings of anthurium andraeanum L. under drought stress. Plant Physiology Communications, 2008, 44(1): 103-105. (in Chinese)

[28]揭雨成, 黄丕生, 李宗道. 干旱胁迫下苎麻的生理生化变化与抗旱性的关系. 中国农业科学, 2000, 33(6): 33-39.

Jie Y C, Huang P S, Li Z D. Relationship between physiological-biochemical changes of ramie under drought stress and drought resistance. Scientia Agricultura Sinica, 2000, 33(6): 33-39. (in Chinese)

[29]原向阳, 郭平毅, 张丽光, 王  鑫, 赵  锐, 郭  秀, 宋喜娥.干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响.中国农业科学, 2010, 43(3): 698-705.

Yuan X Y, Guo P Y, Zhang L G, Wang X, Zhao R, Guo X, Song X E. Glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidation in leaves of glyphosate- resistant soybean [Glycine max(L.) Merr.] seedlings. Scientia Agricultura Sinica, 2010, 43(3): 698-705. (in Chinese)

[30]王启明. 干旱胁迫对大豆苗期叶片保护酶活性和膜脂过氧化作用的影响.农业环境科学学报, 2006, 25(4): 918-921.

Wang Q M. Effects of drought stress on protective enzymes activities and membrane lipid peroxidation in leaves of soybean seedlings. Journal of Agro-Environment Science, 2006, 25(4): 918-921.(in Chinese)

[31]葛体达, 隋方功, 白莉萍, 吕银燕, 周广胜. 水分胁迫下夏玉米根叶保护酶活性变化及其对膜脂过氧化作用的影响.中国农业科学, 2005, 38(5): 922-928.

Ge T D, Sui F G, Bai L P, Lü Y Y, Zhou G S. Effects of water stress on the protective enzyme activities and lipid peroxidation in roots and leaves of summer maize. Scientia Agricultura Sinica, 2005, 38(5): 922-928. (in Chinese)

[32]王之焕, 于英梅, 张晓明. 低温弱光下壳聚糖对番茄幼苗生理指标的影响.吉林农业大学学报, 2011, 33(1): 42-46. 

Wang Z H, Yu Y M, Zhang X M. Effects of chitosan on physiological indices of tomato seedlings under low temperature and poor light level. Journal of Jilin Agricultural University, 2011, 33(1): 42-46. (in Chinese)
[1] NING RuoYun, YIN YuQi, SHEN JianGuo, ZHANG ShuLing, GONG MeiFang, GAO FangLuan. The Phylogeographic History of Pepper Mild Mottle Virus [J]. Scientia Agricultura Sinica, 2026, 59(3): 543-555.
[2] WANG MengYuan, WEI QianRui, LI HaiYan, YANG QiaoMin, YU Jun, HUANG Wei, LU MingHui. Functional Analysis of MADS-box Transcription Factor Gene CaAGL61 in Heat Tolerance of Pepper [J]. Scientia Agricultura Sinica, 2025, 58(8): 1604-1616.
[3] ZHENG Yu, CHEN Yi, TI JinSong, SHI LongFei, XU XiaoBo, LI YuLin, GUO Rui. Evaluation of Carbon Footprint and Economic Benefit of Different Tobacco Rotation Patterns [J]. Scientia Agricultura Sinica, 2025, 58(4): 733-747.
[4] LIANG Xue, JIANG Yan, WEI ChangZhou, XUE Bing, LI FangFang, CUI YiRui, ZHANG XiaRan. Research on Soil Moisture Diagnosis Model of Maize Farmland Based on Remote Sensing of Unmanned Aerial Vehicles [J]. Scientia Agricultura Sinica, 2025, 58(23): 4979-4992.
[5] LI NiFei, YANG QiaoMin, YANG KeCheng, XING YuTeng, WANG MengYuan, ZANG TianBao, LU MingHui. Functional Analysis of CaIAA8, An Interacting Protein of the Autophagy-Related Protein CaATG8c, in the Heat Tolerance of Pepper [J]. Scientia Agricultura Sinica, 2025, 58(22): 4732-4745.
[6] LIU Jing, WANG Hong, ZHANG Lei, XIAO JiuJun, WU JianGao, GONG MingChong. Inversion of Nitrogen Content in Chili Pepper Leaves Based on Hyperspectral Analysis [J]. Scientia Agricultura Sinica, 2025, 58(2): 252-265.
[7] ZHANG Jie, HU ChenXi, QI JianBo, ZHANG YongTai, CHEN YiBo, ZHANG YongJi. Effects of Exogenous Zeatin on Photosynthetic Parameter, Antioxidant System and Expression of Genes Related to Zeatin Synthesis in Pepper Under Low-Temperature Combined with Low-Light Stress [J]. Scientia Agricultura Sinica, 2025, 58(19): 3959-3969.
[8] WU HuiQin, WANG Jing, YANG Yi, LIU XueQing, ZHANG KaiXuan, WANG LuYao, LU JiaWei, ZHAI Yuan, CHENG Yan. Analysis and Evaluation of Fruit Texture Quality of 96 Pepper Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(14): 2854-2868.
[9] XU JiaXin, HUA Nan, WANG YongQiang, XU Hao, LIU Zhen, ZHAO XiaoRui, LI Yue, CHEN QiWei, YE Lin. Response Surface Methodology Optimization of Water, Fertilizer, and Pesticide Coupling on Chili Pepper Growth, Photosynthetic Characteristics, and Root Rot [J]. Scientia Agricultura Sinica, 2025, 58(14): 2869-2884.
[10] LU Lu, LI YiNuo, ZHANG XiuGuo, GAO DanMei, WU FengZhi. Effects of Wheat and Broad Bean Catch Crop and Their Decomposed Liquids on the Growth and Blight of Continuous Cropping Pepper Seedlings [J]. Scientia Agricultura Sinica, 2024, 57(22): 4541-4552.
[11] HE Yong, FAN XiaoZhu, CHEN XinYue, DUAN ShuJing, HU TingTing, XIE RuXue, WANG YuQing, CHEN Jing. Screening and Verification of Pepper Host Factors Interacting with the 126 kDa Protein of Pepper Mild Mottle Virus by Yeast Two-Hybrid System [J]. Scientia Agricultura Sinica, 2024, 57(15): 2986-2996.
[12] GAO ChengAn, WAN HongJian, YE QingJing, CHENG Yuan, LIU ChenXu, HE Yong. Identification and Comparative Analysis of Processed/Fresh-Eating Chili Pepper Fruits at Different Maturation Stages by Metabolomics [J]. Scientia Agricultura Sinica, 2024, 57(12): 2424-2438.
[13] ZHOU DaoMing, SUN Tao, ZHAO YuHong, JIA YuanJie, YANG MingFei, QU Feng, HU XiaoHui. Optimization of Water and Fertilizer Management of Substrate Cultivated Peppers Based on Quality, Yield, and Water and Fertilizer Use Efficiency [J]. Scientia Agricultura Sinica, 2023, 56(12): 2354-2366.
[14] DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong. Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(6): 1189-1198.
[15] LIU RuiYao,HUANG GuoHong,LI HaiYan,LIANG MinMin,LU MingHui. Screening and Functional Analysis in Heat-Tolerance of the Upstream Transcription Factors of Pepper CaHsfA2 [J]. Scientia Agricultura Sinica, 2022, 55(16): 3200-3209.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd