Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (3): 405-413.doi: 10.3864/j.issn.0578-1752.2012.03.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Analysis of Genetic Diversity of Japonica Rice Landrace in China with Microsatellite Marker

 ZHANG  Li-Na, CAO  Gui-Lan, HAN  Long-Zhi   

  1. 1.中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源利用重点开放实验室，北京 100081
  • Received:2011-07-13 Online:2012-02-01 Published:2011-11-28

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Abstract: 【Objective】 The objective of this study is to analyze the genetic diversity, genetic structure and genetic relationship of japonica rice landrace from China, and to provide effective information for hybrid breeding of japonica rice. 【Method】 The genetic diversity, genetic structure and cluster analysis of 187 japonica rice landraces from 17 provinces (cities or municipalities ) of China were discussed using 43 pairs of polymorphic SSR markers.【Result】The total number of alleles detected from all tested 187 accessions was 351, and the alleles per locus ranged from 2 to 21 with the mean value of 8.2. The Nei’s gene diversity indices varied from 0.117 to 0.908 with an average of 0.550. The polymorphism information content (PIC) was changed from 0.114 to 0.902 with the average of 0.523. RM72, RM241, RM219, RM412 and RM232 showed higher genetic diversity than the others. The gene diversity values (He) of japonica rice landrace from southwestern, south and central China were higher than that of north and northeastern China. The He of japonica rice landrace from Yunnan was highest compared to the other provinces, while the lowest were Tianjin and Jilin. The dendrogram of japonica rice landrace was constructed based on Nei’s genetic distance, showed that all rice varieties were subdivided into 8 clusters. The japonica rice landrace of south China contained ⅰ, ⅱ, ⅲ, ⅳ and ⅴ clusters, the north China consisted of ⅵ, ⅶ and ⅷ clusters. The genetic structure analysis was performed based on model, the japonica rice landrace was divided into 11 subgroups including 144 individuals and a mixed group comprising of 43 individuals. 【Conclusion】Compared to north rice region, the south rice region showed significantly higher genetic diversity, and genetic diversity in Southwestern China of rice cropping region was the most among the 5 regions, and the center of origin of Chinese japonica rice landrace is probably Yunnan province. The japonica rice landrace from north and south was largely divergent, and the genetic relationship among japonica rice landrace was partly correlated with the geographical distribution, especially in north japonica rice region. RM72, RM241, RM219, RM412 and RM232 were fit for assessment of the genetic diversity of japonica rice landrace.

Key words: japonica rice, landrace, genetic diversity, genetic structure

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