大豆二粒荚库容含量的多年QTL分析

doi:10.3864/j.issn.0578-1752.2011.22.002

Abstract

Abstract: 【Objective】The objective of this study is to locate consensus QTLs of two-seed pod length and width for breeding new varieties with high two-seed pod storage capacity and increase yield of soybean. 【Method】In order to find out the steady and repeatable QTLs of these traits, a F2:14-F2:18 RIL population containing 147 lines derived from a cross between Charleston as female and Dongnong594 as male parent were used in this experiment. A genetic linkage map was constructed with 164 SSR primers screened in two parents and amplified in 147 lines population. Two-seed pod length and width showing polymorphic in parents were investigated and QTLs were analyzed in one site in the early two years and two sites in the late three years. 【Result】 Nineteen QTLs for the two-seed pod length and width were analyzed with software Windows QTL Cartographer V2.0 by CIM and MIM under different environments, pod length were mapped in the linkage groups A1, B2, C2, D1a, D1b, N and G, 17 QTLs for two-seed pod width were mapped in the linkage groups A1, C2, D1a, D1b, N and H. In these QTLs,7 QTLs for two-seed pod length with linkage primers including Satt200—qTSPL-a1-1—Satt042, Sat_214—qTSPL-d1a-1—Sat_112, Satt198—qTSPL-d1a-3—Satt502, Satt370— qTSPL-d1a-6—Satt402, Sat_092—qTSPL-c2-4—Satt289, Satt277—qTSPL-c2-5—Sct_188 and Satt168—qTSPL-b2-1—Sat_083 could be detected together by CIM and MIM, 1 QTL for two-seed pod width including Satt528—qTSPW-d1a-2—Satt182 could be detected together by two methods. Eight two-seed pod length QTLs including Satt200—qTSPL-a1-1—Satt042, Sat_119— qTSPL-a1-2—Sat_105, Sat_214—qTSPL-d1a-1—Sat_112, Satt220—qTSPL-d1a-4—Sat_162, Satt370—qTSPL-d1a-6—Satt402, Satt168—qTSPL-b2-1—Sat_083, Sat_092—qTSPL-c2-4—Satt289 and Satt277—qTSPL-c2-5—Sct_188 could be detected in more than two years, 4 two-seed pod width QTLs including Satt076—qTSPW-c2-1—Satt072, Satt335—qTSPW-c2-2—Sat_120, Satt200— qTSPW-a1-1—Satt042 and Satt182—qTSPW-d1a-3—Satt584 could be detected in more than two years.【Conclusion】Mapping two-seed pod length and width QTL provide valuable information for soybean molecular marker assistant breeding selection.

Key words: soybean, two-seed pod length, two-seed pod width, QTL analysis

YANG Zhen, SHEN Yan-Ru, HAN Dong-Wei, LIU Chun-Yan, JIANG Hong-Wei, CHEN Qing-Shan, HU Guo-Hua. QTL Analysis of Two-Seed Pod Storage Capacity in Soybean in Different Years[J].Scientia Agricultura Sinica, 2011, 44(22): 4560-4569.

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QTL Analysis of Two-Seed Pod Storage Capacity in Soybean in Different Years

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