Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3613-3621.doi: 10.3864/j.issn.0578-1752.2011.17.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Effect of Undernutrition and Compensation on the Growth of Small Intestinal Mucosa in Lambs

LIU  Xiao-Gang, LI  Da-Biao, HOU  Xian-Zhi, KAO  Gui-Lan, WANG  Hai-Rong, YANG  Jin-Li, ZHANG  Chong-Zhi, XIA  Wei   

  1. 1.内蒙古农业大学动物科学学院
    2.内蒙古农业大学兽医学院
    3.内蒙古农业大学生命科学学院
  • Received:2010-08-02 Revised:2011-03-31 Online:2011-09-01 Published:2011-06-17

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Abstract: 【Objective】 The effects of undernutrition (0-60 d) and compensation (60-150 d) on live weight (BW) and development of small intestinal mucosa in lambs were investigated. 【Method】 Eighty Wu Zhu Mu Qin lambs at average live weight (14.72 ± 1.10) kg, were divided into control group (CG), restricted group 1 (RG1), restricted group 2 (RG2) and restricted group 3 (RG3). Dietary nutrition level were (ME: 10.61 MJ•kg-1, CP: 150.8 g•kg-1), (ME: 10.49 MJ•kg-1, CP: 92.4 g•kg-1), (ME: 8.36 MJ•kg-1, CP: 150.1 g•kg-1) and (ME: 8.10 MJ•kg-1, CP: 62.7 g•kg-1) during undernutrition period for CG, RG1, RG2 and RG3, respectively. However, in nutritional compensation phase all groups were offered the same levels diet (ME: 9.77 MJ•kg-1, CP: 124.8 g•kg-1). Weight of lambs was measured every week. Four lambs of each group were slaughtered at 0 d (at the beginning of undernutrition period), 60 d (at the end of undernutrition period) and 150 d (the end of nutrition compensation period). The samples of duodenum, jejunum and mid-ileum were taken and fixed by using paraformaldehyde phosphate buffer. After HE staining, morphological structure of intestinal mucosa were observed under light microscope. 【Result】 The results showed that at the end of nutrition restriction period, the average live weight of RG1, RG2 (P＜0.05) and RG3 (P＜0.01) was significantly lower than that of CG. However, during the nutrition compensation period, the growth rate of RG3 was significantly higher than that of CG (P＜0.05). Development and growth of the small intestine mucosa were affected by nutrition level of diets. The energy restricted group (RG2) showed better compensatory potency than that of the protein restricted group (RG1). 【Conclusion】 The results indicated that after restriction by different levels of energy and protein all lambs showed compensatory effect in live weight and small intestinal mucosa growth. The development of small intestine mucosa was more prone to be influenced by dietary protein content other than energy concentration. After 60 d restriction by the nutrition level of ME: 8.36 MJ•kg-1, CP: 150.1 g•kg-1, the development of small intestinal mucosa of RG2 reached complete compensation at the end of nutrition compensation period, and nutrients absorption and growth rate also increased correspondingly.

Key words: undernutrition, compensation, lamb, small intestine, mucosal structure, body weight

[1]Kagnoff M F. Mucosal immunology: new frontiers. Immunology Today, 1996, 17(2): 57-59.

[2]陈付菊, 陈耀星, 王子旭, 刘云芳. 新生犊牛小肠黏膜结构的早期发育及上皮内淋巴细胞和杯状细胞的数量变化. 中国兽医科学, 2007, 37(6): 519-523.

Chen F J, Chen Y X, Wang Z X, Liu Y F. Early development of mucosal structure and quantitative changes of intraepithelial lymphocyte and goblet cell in the small intestine of neonatal calf. Veterinary Science in China, 2007, 37(6): 519-523. (in Chinese)

[3]孙德文, 詹  勇, 许梓荣. 日粮营养调控动物肠道黏膜免疫研究.中国畜牧杂志, 2004, 5(40): 36-39.

Sun D W, Zhan Y, Xu Z R. Study on dietary nutrients regulation animal intestinal mucosal immune. Chinese Journal of Animal Science, 2004, 5(40): 36-39. (in Chinese)

[4]高  峰, 侯先志, 刘迎春. 妊娠后期限饲母羊血液理化指标变化对其胎儿生长发育的影响. 中国科学, 2007, 37(5): 562-567.

Gao F, Hou X Z, Liu Y C. The effect of received by ewes at late pregnancy on changes in blood parameters of their lambs in development growth. Science in China, 2007, 37(5): 562-567. (in Chinese)

[5]Hayden J M, Williams J E, Collier R J. Plasma growth hormone, insulin-like growth factor, insulin, and thyroid hormone association with body protein and fat accretion in steers undergoing compensatory gain after dietary energy restriction. Journal of Animal Science, 1993, 71: 3327-3338.

[6]Drouillard J S, Ferrell C L, Klopfenstein T J, Britton R A. Compensatory growth following metabolizable protein or energy restrictions in beef steers. Journal of Animal Science, 1991, 69: 811-818.

[7]Drouillard J S, Klopfenstein T J, Britton R A, Bauer M L, Gramlich S M, Wester T J, Ferrell C L. Growth, body composition, and visceral organ mass and metabolism in lambs during and after metabolizable protein or net energy restrictions. Journal of Animal Science, 1991, 69: 3357-3375.

[8]高  峰, 侯先志, 敖长金, 赵志恭, 吴庶青, 王志新. 妊娠后期不同营养水平限制饲养母羊对其羔羊补偿生长期血中胰岛素、T3、T4浓度的影响. 畜牧兽医学报, 2004, 35(3): 270-275.

Gao F, Hou X Z, Ao C J, Zhao Z G, Wu S Q, Wang Z X. The effect of different undernutrition received by ewes at late pregnancy on serum insulin, T3, T4 concentrations of their lambs in compensatory growth. Acta Veterinaria et Zootechnica Sinica, 2004, 35(3): 270-275. (in Chinese)

[9]张宏福, 张子仪．动物营养参数与饲养标准．北京：中国农业出版社, 1998．

Zhang H F, Zhang Z Y. Animal Nutrition Parameters and the Breed Standard. Beijing: China Agriculture Press, 1998. (in Chinese)

[10]高  峰. 妊娠后期限饲母羊对其胎儿生长发育及出生后羔羊补偿生长的影响[D]. 内蒙古: 内蒙古农业大学, 2006.

Gao F. Effect of maternal undernutrition during late pregnancy on ovine fetal development and subsequent compensatory growth of postnatal lambs[D]. Inner Mongolian: Inner Mongolian Agricultural University, 2006. (in Chinese)

[11]Gomez-Pasten M, Mora O, Pedraza-Chaverri J, Shimada A. The effect of a long term feed restriction on metabolism and tissue compostition of goats. Journal of Agricultural Science, 1999, 132: 227-232.

[12]Reynolds C K, Tyrrell H F, Reynolds P J. Effects of diet forage-to-concentrate ratio and intake on energy metabolism in growing beef heifers: whole body energy and nitrogen balance and visceral heat production. Journal of Nutrition, 1991, 121: 994-1003.

[13]Brandstetter A M, Pfaffl M W, Hocquette J F, Gerrard D E, Picard B, Geay Y, Sauerwein H. Effects of muscle type, castration, age, and compensatory growth rate on androgen receptor mRNA expression in bovine skeletal muscle. Journal of Animal Science, 2000, 78: 629-637.

[14]Han C H, Austin K J, Nathanielsz P W, Ford S P, Nijland M J, Hansen T R. Maternal nutrient restriction alters gene expression in the ovine fetal heart. The Journal of Physiology, 2004, 558(1): 111-121.

[15]Ryan W J, Williams I H, Moir R J. Compensatory growth in sheep and cattle.II.Changes in body composition and tissue weights. Australian Journal of Agricultural Research, 1993, 44: 1623-1633.

[16]杨  倩, 毛卫华, 赵茹茜, 唐晓萍, 陈  杰. 太湖猪与大白猪小肠发育及其免疫功能形态学比较. 南京农业大学学报, 2001, 24(4): 75-78.

Yang Q, Mao W H, Zhao R Q, Tang X P, Chen J. Comparison on the morphology of the developmen of intestine and immunological function between Taihu and Large White pigs. Journal of Nanjing Agricultural University, 2001, 24(4): 75-78. (in Chinese)

[17]周金星, 高登慧, 刘培琼, 毕亚玲. 不同日龄香猪小肠黏膜形态观察. 中国兽医杂志, 2005, 41(12): 11-12.

Zhou J X, Gao D H, Liu P Q, Bi Y L. Morphology of intestinal mucosa of different age swine. Chinese Journal of Veterinary Medicine, 2005, 41(12): 11-12. (in Chinese)

[18]姚浪群, 萨仁娜, 佟建明, 霍启光. 安普霉素对仔猪肠道微生物及肠壁组织结构的影响. 畜牧兽医学报, 2003, 34(3): 250-257.

Yao L Q, Sa R N, Tong J M, Huo Q G. Effect of apramycin on intestinal flora and intestinal morphology of piglets. Acta Veterinaria et Zootechnica Sinica, 2003, 34(3): 250-257. (in Chinese)

[19]Swanson K C, Redmer D A, Reynolds L P, Caton J S. Ruminally undegraded intake protein in sheep fed low-quality forage: effect on weight, growth, cell proliferation, and morphology of visceral organs. Journal of Animal Science, 1999, 77: 198-205.

[20]Caton J S, Reed J J, Aitken R P, Milne J S, Borowicz P P, Reynolds L P, Redmer D A, Wallace J M. Effects of maternal nutrition and stage of gestation on body weight, visceral organ mass, and indices of jejunal cellularity, proliferation, and vascularity in pregnant ewe lambs. Journal of Animal Science, 2009, 87: 222-235.

[21]Reed J J, Ward M A, Vonnahme K A, Neville T L, Julius S L, Borowicz P P, Taylor J B, Redmer D A, Grazul-Bilska A T, Reynolds L P, Caton J S. Effects of selenium supply and dietary restriction on maternal and fetal body weight, visceral organ mass, cellularity estimates, and jejunal vascularity in pregnant ewe lambs. Journal of Animal Science, 2007, 85: 2721-2733.

[22]Scheaffer A N, Caton J S, Redmer D A, Arnold D R, Reynolds L P. Effect of dietary restriction, pregnancy, and fetal type on intestinal cellularity and vascularity in Columbia and Romanov ewes. Journal of Animal Science, 2004, 82: 3024-3033.

[23]McLeod K R, Baldwin R L. Effects of diet forage: concentrate ratio and metabolizable energy intake on visceral organ growth and in vitro oxidative capacity of gut tissues in sheep. Journal of Animal Science, 2000, 78: 760-770. 

[24]Jin L, Reynolds L P, Redmer D A, Caton J S, Crenshaw J D. Effects of dietary fiber on intestinal growth, cell proliferation, and morphology in growing pigs. Journal of Animal Science, 1994, 72: 2270-2278.

[25]王  恬, 许若军, 张元强, 郑春田, 张建成. 酶解乳蛋白质对初生仔猪小肠组织学结构及隐窝细胞增殖的影响. 中国组织化学与细胞化学杂志, 1999, 8(2): 228-235.

Wang T, Xu R J, Zhang Y Q, Zheng C T, Zhang J C. Effects of mild protein pre-hydrol yzation on small intestinal morphology and crypt cell proliferation in neonatal piglets. Chinese Journal of Histochemistry and Cytochemistry, 1999, 8(2): 228-235. (in Chinese)

[26]Anderson T A. Histological and cytological structure of the gastrointestinal tract of the luderick, Girella tricuspidata (Pisces, Kyphosidae), in relation to diet. Journal of Morphology, 1986, 190(1): 109-119.

[27]师昆景, 吴灵英. 肉禽早期肠道发育的营养调控. 家禽科学, 2007, 4: 38-40.

Shi K J, Wu L Y. By nutrition regulated to poultry early enteral growth. Poultry Science, 2007, 4: 38-40. (in Chinese)
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