Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (17): 3622-3633.doi: 10.3864/j.issn.0578-1752.2011.17.015

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Study on the Interspecies Crossbreeding Between Mithun (Bos frontalis) and Zebu (Bos indicus) Cattle

HE  Zhan-Xing, ZHANG  Ji-Cai, XU  Kai-Xing, JIN  Xian-Dong, WANG  Zhe, WANG  An-Kui, HUANG  Mei-Fen, ZHAO  Gang, YANG  Guo-Rong, TU  Xian-Yun, YUAN  Xi-Ping, HUANG  Bi-Zhi   

  1. 1. 云南省草地动物科学研究院
    2. 西北农林科技大学动物科技学院
    3. 云南省泸水县畜牧兽医局
  • Received:2010-07-26 Revised:2010-09-25 Online:2011-09-01 Published:2010-09-27

PDF

1300

Cited

3

Abstract: 【Objective】 The potential of interspecies crossbreeding utilization and its foreground between Mithun (Bos frontalis) and Zebu (Bos indicus) cattle were studied. 【Method】 Firstly, after treated by CUE-MATETM (progesterone-releasing intravaginal device) and PG for synchronization estrous, Brahman cows were inseminated artificially with Mithun’s frozen semen. And gestation length and calving time of cows and calf sex ratio were investigated. Besides, the body weight of Mithun and Brahman crossbreed at birth, 6 months, 12 months and 18 months of age were measured and karyotype and reproductive performance of hybrids F1 (Mithun×Brahman) and so on were analyzed and investigated. 【Result】 The rates of synchronization estrous, pregnancy and calving of Brahman cow were 83.87%, 60.56% and 45.07%, respectively. The average gestation length was (290.83±5.26) d (n=24). The percentage of female calf in crossbred F1 (Mithun×Brahman) was 71.88%，higher than those of Mithun and Brahman (P＜0.05), and significantly higher than those of crossbred F1 (Mithun×Yellow cattle) and BMY cattle, respectively (P＜0.01). The average body weight at birth, 6 months, 12 months and 18 months of crossbred F1 (Mithun×Brahman)(n=29) was (26.69±4.84) kg, (153.08±37.58) kg, (219.34±31.31) kg and (302.06±28.92) kg, respectively, which were 68.18%, 37.12%, 45.07% and 40.13% higher than those of Mithun (P＜0.01), but 14.40% (P＜0.01), 5.23% (P＞0.05), 13.12% (P＜0.01) and 12.76% (P＜0.01) lower than those of Brahman. The average daily gain (ADG) of crossbred F1 (Mithun×Brahman) from birth to 18 months of age was (503.09±44.91) g and (502.89±53.54) g for male and female，which were 35.98% and 39.85% higher than those of male and female of Mithun (P＜0.01), but 16.49% and 6.63% lower than those of male (P＜0.01) and female (P＞0.05) of Brahman. The chromosome numbers of Mithun, Brahman and their crossbred F1 (Mithun×Brahman) were 2n=58, 2n=60 and 2n=59, respectively. The average age and body weight of crossbred F1 (Mithun×Brahman) at first pregnancy and their primiparous age were (16.00±2.83) months, (342.50±30.41) kg and (26.00±2.83) months, respectively, which gave one calf per year. The semen collection of 5 crossbred F1 (Mithun×Brahman) at 15-24 months of age was failed by electro-ejaculation treatment.【Conclusion】Interspecies crossbreeding of Mithun×Zebu performed better in rapid growth, wide adaptation and significant heterosis. And the crossbred female F1 was of better reproductive capacity, precocity and high reproductive efficiency. However, the fertility of male crossbred F1 will remain to be studied in future.

Key words: Gayal or Mithun (Bos frontalis), Brahman (Bos indicus), Interspecies crossbreeding, growth performance, heterosis

[1]Bhusan S, Rajkhowa C, Bujarbaruah K M. Body weight and growth pattern in mithun (Bos frontalis). Indian Journal of Animal Sciences, 2005, 75(10): 1186-1188.

[2]兰  宏, 熊习昆, 林世英, 刘爱华, 施立明. 云南黄牛和大额牛mtDNA多态性研究. 遗传学报, 1993, 20(5): 419-425.

Lan H, Xiong X K, Li S Y, Liu A H, Shi L M. Mitochondrial DNA polymorphism of cattle (Bos taurs) and mithun (Bos frontalis) in Yunnan province. Acta Genetica Sinica, 1993, 20(5): 419-425. (in Chinese)

[3]王兰萍, 耿荣庆, 常  洪, 冀德君, 李永红, 常春芳. 大额牛起源与系统地位的遗传学分析. 云南农业大学学报: 自然科学版, 2009, 24(2): 231-234.

Wang L P, Geng R Q, Chang H, Ji D J, Li Y H, Chang C F. Genetic analysis on origin and phylogenetic status of Gayal (Bos frontalis). Journal of Yunnan Agricultural University: Natural Sciences, 2009, 24(2): 231-234. (in Chinese)

[4]苟  潇, 王永奇, 杨舒黎, 邓卫东, 毛华明. 从mtDNA和SRY基因多态揭示云南大额牛杂交起源. 中国农业大学学报: 自然科学版, 2009, 14(1): 31-37.

Gou X, Wang Y Q, Yang S L, Deng W D, Mao H M. Hybrid origin of Yunnan Gayal revealed by mtDNA and SRY gene sequence variation. Journal of China Agricultural University: Natural Sciences, 2009, 14(1): 31-37. (in Chinese)

[5]冀德君, 常春芳, 常  洪, 耿荣庆, 李永红. 中国牛亚科6个物种MSTN基因外显子2多态性及分化研究. 中国畜牧杂志, 2009(45): 1-3.

Ji D J, Chang C F, Chang H, Geng R Q, Li Y H. Polymorphism and phylogenetic relationship of myostatin gene exon 2 in 6 bovinae species in China. Chinese Journal of Animal Science, 2009(45): 1-3. (in Chinese)

[6]单祥年, 陈宜峰, 罗丽华, 曹筱梅, 宋继志, 曾养志. 中国黄牛属(Bos)五个种的染色体比较研究. 动物学研究, 1980, 1(1): 75-81.

Shan X N, Chen Y F, Luo L H, Cao X M, Song J Z, Zeng Y Z. Comparative studies on the chromosomes of five species of cattle of the Bos in China. Zoological Research, 1980, 1(1): 75-81. (in Chinese)

[7]Ma G L, Chang H, Li S P, Chen H Y, Ji D J, Geng R Q, Chang C F, Li Y H. Phylogenetic relationships and status quo of colonies for Gayal based on analysis of cytochrome b gene partial sequences. Journal of Genetics and Genomics, 2007, 34(5): 413-419.

[8]云南省家畜家禽品种志编写委员会. 云南省家畜家禽品种志. 昆明: 云南科技出版社, 1987,.

Editorial Committee of the “Breeds of Domestic Animal and Poultry” in Yunnan. Breeds of Domestic Animal and Poultry in Yunnan. Kunming: Yunnan Science and Technology Press, 1987.

[9]Heli T, Saikaia S, Bora N N. MITHUN: the magnificient bos species of North-East India：its physical characteristics. Indian Journal of Animal, Production, Management, 1996, 12(3, 4): 144-153.

[10]Mondal S K, Bujarbaruah K M, Khaie K, Borah I P. Body conformation traits in mithun (Bos frontalis). Indian Journal of Animal Sciences, 2004, 74(1): 84-87.

[11]和占星, 亐开兴, 袁希平, 桑业华, 马文张, 张继才, 李子良. 凤凰山迁地保种大额牛的外貌特征及主要习性. 云南农业大学学报: 自然科学版, 2009, 24(2): 225-230.

He Z X, Qu K X, Yuan X P, Sang Y H, Ma W Z, Zhang J C, Li Z L. Appearance character and major behavior of gayal (Bos frontalis) on the conservation ex situ in Phenix Mountain. Journal of Yunnan Agricultural University: Natural Sciences, 2009, 24(2): 225-230. (in Chinese)

[12]王永奇, 苟  潇, 文际坤, 毛华明, 苏八七, 张  勇. 独龙牛生长发育规律的研究. 云南农业大学学报: 自然科学版, 2010, 25(2): 63-68.

Wang Y Q, Gou X, Wen J K, Mao H M, Su B Q, Zhang Y. Research on growth and development of Dulong Cattle. Journal of Yunnan Agricultural University: Natural Sciences, 2010, 25(2): 63-68. (in Chinese)

[13]葛长荣, 陈  韬, 袁希平. 大额牛、云南瘤牛屠宰性能研究. 黄牛杂志, 1996, 22(3): 31-33.

Ge C R, Chen T, Yuan X P. A study on slaughtering performance of Yunnan Zebu and width forehead cattle (Bos frontalis). Journal of Yellow Cattle Science, 1996, 22(3): 31-33. (in Chinese)

[14]葛长荣, 田允波. 大额牛、云南瘤牛横纹肌组织形态学研究. 西南民族学院学报: 自然科学版, 1996, 22(1): 33-36.

Ge C R, Tian Y B. Studies on the striated muscles of the the Gayal and the Zebu. Journal of Southwest Nationalities College: Natural Sciences, 1996, 22(1): 33-36. (in Chinese)

[15]Giasuddin M, Huque K S, Alam J. Reproductive potentials of mithun (Bos frontalis) under semi-intensive management. Asian-Australasian Journal of Animal Sciences, 2003, 16(3): 331-334.

[16]Dhali A, Mishra D P, Karunakaran M, Mech A, Rajkhowa C. Secretion patterns of luteinising hormone, follicle-stimulating hormone and 17β-oestradiol during oestrus and the mid-luteal phase of the oestrous cycle in mithun (Bos frontalis). Reproduction, Fertility and Development, 2006, 18: 619-626.

[17]He Z X, Yuan X P, Qu K X, Huang B Z, Ma W Z, San Y H, Li Z L, Yang S H. Investigation of calving behavior of Gayal (Bos frontalis) under semi-wild in China. Reproduction in Domestic Animals, 2008, 43(Suppl.3): 217-218.

[18]Yang S L, Ma S C, Chen J, Mao H M, He Y D, Xi D M, Yang L Y, He T B, Deng W D. Bacterial diversity in the rumen of Gayal (Bos frontalis), swamp buffaloes (Bubalus bubalis) and holstein cow as revealed by cloned 16S rRNA gene sequences. Molecular Biology Reports, 2010, 37: 2063-2073.

[19]和协超, 沈  放, 季维智, 赵家才, 刘国璋, 杨国荣, 和占星. 电刺激采集大额牛精液及其低温冷冻的初步研究. 兽类学报, 2000, 20(3): 232, 239-240.

He X C, Shen F, Ji W Z, Zhao J C, Liu G Z, Yang G R, He Z X. Successful electroe jaculation and semen cryopreservation of Bos frontalis. Acta Theriologica Sinica, 2000, 20(3): 232, 239-240. (in Chinese)

[20]Mondal M, Karunakaran M, Lee K B, Rajkhowa C. Characterization of Mithun (Bos frontalis) ejaculates and fertility of cryopreserved sperm. Animal Reproduction Science, 2010, 118: 210-216.

[21]Dhali A, Karunakaran M, Mech A, Nath N, Prakash B, Rajkhowa C, Mishra D P. Birth of the first Mithun (Bos frontalis) calf through artificial insemination. Animal, 2008, 2(6): 879-882.

[22]Winter H, Mayr B, Schleger W, Dworak E, Krutzler J. Genetic characterisation of the mithun (Bos frontalis) and studies of spermatogenesis, blood groups and haemoglobins of its hybrids with Bos indicus. Research in Veterinary Science, 1986, 40(1): 8-17.

[23]Huque K S, Rahman M M, Jalil M A. Study on the growth pattern of mithuns (Bos frontalis) and their crossbred calves. Asian-Australasian Journal of Animal Sciences, 2001, 14(9): 1245-1249.

[24]范江平, 叶绍辉, 葛长荣, 和天宝, 黄玉路. 杂交大额牛肉质特性研究初报. 云南农业大学学报: 自然科学版, 2005, 20(4): 600-602.

Fan J P, Ye S H, Ge C R, He T B, Huang Y L. Research on the meat characteristic of crossbred mithun. Journal of Yunnan Agricultural University: Natural Sciences, 2005, 20(4): 600-602. (in Chinese)

[25]和占星, 张继才, 亐开兴, 黄必志, 袁希平, 赵  刚, 黄梅芬, 张  勇, 何永珍. 大额牛冻精在瘤牛超排中的应用效果及F1代的亲子鉴定. 中国农业科学, 2008, 41(8): 2436-2441.

He Z X, Zhang J C, Qu K X, Huang B Z, Yuan X P, Zhao G, Huang M F, Zhang Y, He Y Z. Application of frozen sperm of Mithun in superovulation of Zebu and parentage identification of crossbred F1(Mithun×Brahman). Scientia Agricultura Sinica, 2008, 41(8): 2436-2441. (in Chinese)

[26]He Z X, Suzuki T, He X C, Zhan J C, Huang B Z. Seasonal effect of superovulation for beef cattle at the subtropical grassland//Agriculture and Animal Husbandry College of Qinghai University, ed. The 13th International Congress on Biotechnology in Animal Reproduction. Xining: Qinghai People’s Publishing House, 2006.

[27]Messine O, Schwalbach L J M, Mbah D A, Ebangi A L. Non-genetic factors affecting gestation length and postpartum intervals in gudali Zebu cattle of the Adamawa Highlands of Cameroon. Tropicultura, 2007, 25(3): 129-133.

[28]Torell R. Gestation length of beef cows could signal dystocia problems. www.Western Farmer Stockman. Com October 2009: 32.

[29]李永海, 段志远, 孟宪印. 精氨酸对奶牛受胎率及后代性别的影 响. 中国兽医科技, 1994, 24(2): 27-28.

Li Y H, Duan Z Y, Meng X Y. Effect of arginine on offspring sex of milk cow. China Veterinary Medicine Technology, 1994, 24(2): 27-28. (in Chinese)

[30]Gutiérrez-Adán A J, Granados B, Pintado, de La Fuente J. Influence of glucose on the sex ratio of bovine IVM/IVF embryos cultured in vitro. Reprodction, Fertility and Development, 2001, 13(5-6): 361-365.

[31]Lechniak D, Strabel T, Bousquet D, King A W. Sperm pre-incubation prior to insemination affects the sex ratio of bovine embryos produced in vitro. Reproduction in Domestic Animals, 2003, 38(3): 224-227.

[32]James W H. Evidence that mammalian sex ratios at birth are partially controlled by parental hormone levels at the time of conception. Journal of Theoretical Biology, 1996, 180(4): 271-286.

[33]郭爱朴. 牦牛、黄牛及其杂交后代犏牛的染色体比较研究. 遗传学报, 1983, 10(2): 137-143.

Guo A P. A comparative study on chromosomes of oxen (Bos taurus), yaks (Bos grunnieus) and their hybrids. Acta Genetica Sinica, 1983, 10(2): 137-143. (in Chinese)

[34]张容昶, 段李成. 对牦牛和普通牛种间杂种公牛精子生成水平的研究. 畜牧兽医学报, 1991, 22(3): 231-234.

Zhang R C, Duan L C. A study on the sperm productivity of species hybrid bull between yaks and cattle. Acta Veterinaria et Zootechnica Sinica, 1991, 22(3): 231-234. (in Chinese)

[35]董丽艳, 李齐发, 屈旭光, 李隐侠, 李新福, 徐洪涛, 谢  庄. 黄牛、牦牛和犏牛睾丸组织中Cdc2、Cdc25A基因mRNA表达水平. 遗传, 2009, 31(5): 495-499.

Dong L Y, Li Q F, Qu X G, Li Y X, Li X F, Xu H T, Xie Z. Expression levels of Cdc2 and Cdc25A mRNA in cattle, yak, and cattle-yak testis. Hereditas, 2009, 31(5): 495-499. (in Chinese)

[36]黄右军, 尚江华, 梁梦玫, 张秀芳, 黄芬香. 河流型水牛与沼泽型杂交后代(2n=49)染色体遗传与繁殖力的研究. 遗传, 2003, 25(2): 155-159.

Huang Y J, Shang J H, Liang M M, Zhang X F, Huang F X. Studies of chromosomal heredity and fertility of progenies (2n=49) crossed between river and swamp buffalo. Hereditas, 2003, 25(2): 155-159. (in Chinese)

[37]Vadhanakul N, Tunwattana W, Sekkasiddhi P, Tansatit M, Chavananikul V. Karyotype of crossbred progeny born to the interspecies hybridization of wild and zebu cattle with reference to their fertility and growth performance. Thai Journal Veterinary, Medicine, 2004, 34(1): 73-81.

[38]赵志军, 赵  刚, 余  梅, 金显栋, 黄必志. 云南引进婆罗门牛纯种繁育效果研究. 中国畜牧杂志, 2009(增刊): 193-196.

ZhaoZ J, Zhao G, Yu M, Jin X D, Huang B Z. Study on effect of pure breed on introduce into Brahman in Yunnan. Chinese Journal of Animal Science, 2009(Suppl.): 193-196. (in Chinese)

[39]Newman S, Deland M P. Lifetime productivity of crossbred cows 2. Age and weight at first oestrus, calf birth weight, assisted calvings, calving interval and reproduction rate. Australian Journal of Experimental Agriculture, 1991, 31: 293-300.

[40]Beate D Scherf. World Watch List for Domestic Animal Diversity. 3rd edn. Rome: Food and Agriculture Organization of the Untied Nations, 2000, 8-584.

[41]任建华. “抗旱王”肉用牛简介. 浙江畜牧兽医, 1981, 1: 19.

Ren J H. Brief introduction in “drought master” beef cattle. Zhejiang Journal of Animal Science and Veterinary, 1981, 1: 19. (in Chinese)

[42]O’Connor S F, Tatum J D, Wulf D M, Green R D, Smith G C. Genetic effects on beef tenderness in Bos indicus composite and Bod taurus cattle. Journal of Animal Science, 1997, 75(7): 1822-1830.
[1] XIE LingLi,WEI DingYi,ZHANG ZiShuang,XU JinSong,ZHANG XueKun,XU BenBo. Dynamic Changes of Gibberellin Content During the Development and Its Relationship with Yield of Brassica napus L. [J]. Scientia Agricultura Sinica, 2022, 55(24): 4793-4807.
[2] CHE DaLu,ZHAO LiChen,CHENG SuCai,LIU AiYu,LI XiaoYu,ZHAO ShouPei,WANG JianCheng,WANG Yuan,GAO YuHong,SUN XinSheng. Effect of Litter Bed on Growth Performance and Odor Emission in Fattening Lamb [J]. Scientia Agricultura Sinica, 2022, 55(24): 4943-4956.
[3] LIU WangJing,TANG DeFu,AO ChangJin. Effect of Allium mongolicum Regel and Its Extracts on the Growth Performance, Carcass Characteristics, Meat Quality and Serum Biochemical Indices of Captive Small-Tailed Han Sheep [J]. Scientia Agricultura Sinica, 2022, 55(17): 3461-3472.
[4] CHEN ZhiMin,CHANG WenHuan,ZHENG AiJuan,CAI HuiYi,LIU GuoHua. Effect of Expanded Feather Powder on Growth Performance, Slaughter Performance and Serum Biochemical Index of Broiler [J]. Scientia Agricultura Sinica, 2022, 55(13): 2643-2653.
[5] WANG JinFei,YANG GuoYi,FAN ZiHan,LIU Qi,ZHANG PengCheng,REN YouShe,YANG ChunHe,ZHANG ChunXiang. Effects of Whole Plant Corn Silage Ratio in Diet on Growth Performance, Rumen Fermentation, Nutrient Digestibility and Serological Parameters of Dorper×Hu Crossbred Female Lambs [J]. Scientia Agricultura Sinica, 2021, 54(4): 831-844.
[6] LI JiangLing,YANG Lan,RUAN RenWu,LI ZhongAn. Analysis of Photosynthetic Characteristics of Hybrid Wheat at Seedling Stage and Its Use for Early Prediction of Strong Heterosis Combinations [J]. Scientia Agricultura Sinica, 2021, 54(23): 4996-5007.
[7] ZHANG Lan,WANG LiangZhi,HUANG YanLing,LIAO XiuDong,ZHANG LiYang,LÜ Lin,LUO XuGang. Effects of Dietary Supplemental Pattern of Trace Eloments on the Growth Performance, Carcass Traits and Meat Quality of Broilers [J]. Scientia Agricultura Sinica, 2021, 54(22): 4906-4916.
[8] LIU Jiao,CHEN ZhiMin,ZHENG AiJuan,LIU GuoHua,CAI HuiYi,CHANG WenHuan. Effects of Glucose Oxidase on Growth Performance, Immune Functions and Intestinal Health of Ducks Challenged by Escherichia coli [J]. Scientia Agricultura Sinica, 2021, 54(22): 4917-4930.
[9] WANG Chen,ZHANG HongWei,WANG HuCheng,SUN XiaoPing,LI FaDi,YANG BoHui. Energy and Protein Requirements of Alpine Merino Growing Sheep [J]. Scientia Agricultura Sinica, 2021, 54(16): 3537-3548.
[10] HUANG WenQin,LÜ XiaoKang,ZHUANG YiMin,CUI Kai,WANG ShiQing,DIAO QiYu,ZHANG NaiFeng. The Effects of Early Weaning and NDF Levels of Finishing Diets on Growth Performance, Nutrient Digestion and Metabolism of Hu Lambs [J]. Scientia Agricultura Sinica, 2021, 54(10): 2217-2228.
[11] YANG YunYan,WANG QiYan,PENG DiWei,PAN YiFan,GAO XiaoMei,XUAN ZeYi,CHEN ShaoMei,ZOU CaiXia,CAO YanHong,LIN Bo. Effects of Cinnamaldehyde on Growth Performance,Health Status, Rumen Fermentation and Microflora of Dairy Calves [J]. Scientia Agricultura Sinica, 2021, 54(10): 2229-2238.
[12] ZHANG MeiQi,LI Yan,LI ShuJing,GAO YanXia,LI JianGuo,CAO YuFeng,LI QiuFeng. Effects of Dietary Energy Levels on Production Performance, Blood Index, Slaughter Performance and Meat Quality of Holstein Steers [J]. Scientia Agricultura Sinica, 2021, 54(1): 203-212.
[13] ZHAO JiuRan, LI ChunHui, SONG Wei, LIU XinXiang, WANG YuanDong, ZHANG RuYang, WANG JiDong, SUN Xuan, WANG XiaQing. Heterosis and Genetic Recombination Dissection of Maize Key Inbred Line Jing2416 [J]. Scientia Agricultura Sinica, 2020, 53(22): 4527-4536.
[14] LI YongXiang,LI ChunHui,YANG JunPin,YANG Hua,CHENG WeiDong,WANG LiMing,LI FengYan,LI HuiYong,WANG YanBo,LI ShuHua,HU GuangHui,LIU Cheng,LI Yu,WANG TianYu. Genetic Dissection of Heterosis for Huangzaosi, a Foundation Parental Inbred Line of Maize in China [J]. Scientia Agricultura Sinica, 2020, 53(20): 4113-4126.
[15] KONG FanLin,LI Yuan,TANG MengQi,MA ManPeng,FU Tong,DIAO QiYu,CHENG SiYuan,TU Yan. Effects of Amino Acid Deficiency on Growth Development, Dietary Nutrients Digestion and Metabolism in Heifers [J]. Scientia Agricultura Sinica, 2020, 53(2): 418-430.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd