Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (13): 2641-2649 .doi: 10.3864/j.issn.0578-1752.2011.13.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity Analysis of Wild Auricularia auricula Strains in Daxing’anling Area Using SRAP Markers

LIU Hua-jing; XU Xiu-hong; JIANG Ting-bo   

  1. 东北农业大学资源与环境学院
  • Received:2010-08-23 Revised:2010-09-12 Online:2011-07-01 Published:2011-07-01

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Abstract: 【Objective】Genetic diversity of wild Auricularia auricula strains in Daxing’anling area was analyzed. 【Method】Using PCR-SRAP system, 9 pairs of primers on 18 wild strains and 6 cultivated ones were selected in expansion, and through clustering method its genetic diversity was analyzed.【Result】By PCR amplification, 90 fragments were amplified. Polymorphic bands were 78 which accounted for 87.0% in the total amplified fragments. The number of amplified and polymorphic fragments was 10.00 and 8.67 per primer pair, respectively. The PIC (polymorphism information content) value of these markers varied from 0.051 to 0.918, averaging 0.683. The genotypes revealed by each marker were 15.78 in average. According to the analysis results, the similarity coefficient was at the level of 0.63. They were divided into 5 groups.【Conclusion】There is an obvious genetic diversity in wild Auricularia auricula strains and cultivated ones, this technique can be used to analyze the genetic diversity in the future study.

Key words: Auricularia auricula, SRAP, genetic diversity

CLC Number: 

  • S646

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