Loading...

Table of Content

    01 July 2011, Volume 44 Issue 13
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Genetic Analysis and Molecular Mapping of the Rice Male Sterile Mutant 802A
    SUN Xiao-Qiu, FU Lei, WANG Bing, XIAO Yun-Hua, DENG Xiao-Jian
    Scientia Agricultura Sinica. 2011, 44(13):  2633-2640 .  doi:10.3864/j.issn.0578-1752.2011.13.001
    Abstract ( 854 )   PDF (404KB) ( 877 )   Save
    Related Articles | Metrics
    【Objective】A male sterile mutant 802A was discovered in the F8 population from the crosses between two indica lines, i.e. Shuihui 527 and Shuhui 881. The objective of this study is to conduct phenotypic observation, genetic analysis and molecular mapping of the 802A mutant. 【Method】The pollen fertility, phenotypes and major agronomic traits of the mutant were observed. Genetic mapping was conducted by using molecular markers and the F2 mapping population from 802A/Ⅱ-32B and 802A/02428. 【Result】The anther of the 802A mutant was slender and milky-yellow, and remained unbroken after flowering. Its pollen primarily belonged to typical abortion type. Meanwhile, the mutant showed that the glumes were thinned and twisted; the flag leaves were narrowed, shortened and frizzled; the basal part of panicles was enclosed in the sheath. Genetic analysis suggested that the male sterility of the 802A mutant was controlled by a pair of recessive nuclear genes. The gene for male sterility is located on the long arm of rice chromosome 3, nearby SSR marker RM3513, between InDel markers S2 and S5. The genetic distances from the target gene to the two InDel markers were 0.6 and 0.3 cM, respectively. At the same time, the mutant gene co-segregated with InDel markers S3 and S4 in 167 F2 male sterile individuals from the mapping population.【Conclusion】Compared with the known male sterile genes, the mutant gene of 802A was considered to be a novel recessive male sterile gene in rice, and was designated tentatively as ms92(t).
    Genetic Diversity Analysis of Wild Auricularia auricula Strains in Daxing’anling Area Using SRAP Markers
    LIU Hua-Jing, XU Xiu-Hong, JIANG Ting-Bo
    Scientia Agricultura Sinica. 2011, 44(13):  2641-2649 .  doi:10.3864/j.issn.0578-1752.2011.13.002
    Abstract ( 703 )   PDF (504KB) ( 866 )   Save
    Related Articles | Metrics
    【Objective】Genetic diversity of wild Auricularia auricula strains in Daxing’anling area was analyzed. 【Method】Using PCR-SRAP system, 9 pairs of primers on 18 wild strains and 6 cultivated ones were selected in expansion, and through clustering method its genetic diversity was analyzed.【Result】By PCR amplification, 90 fragments were amplified. Polymorphic bands were 78 which accounted for 87.0% in the total amplified fragments. The number of amplified and polymorphic fragments was 10.00 and 8.67 per primer pair, respectively. The PIC (polymorphism information content) value of these markers varied from 0.051 to 0.918, averaging 0.683. The genotypes revealed by each marker were 15.78 in average. According to the analysis results, the similarity coefficient was at the level of 0.63. They were divided into 5 groups.【Conclusion】There is an obvious genetic diversity in wild Auricularia auricula strains and cultivated ones, this technique can be used to analyze the genetic diversity in the future study.
    Development of Unigene Derived Microsatellite (UGMS) Markers in Panax ginseng
    WU Tian-Shu, LIANG Cui, LI Hong-Bo, PU Zhong-Yun
    Scientia Agricultura Sinica. 2011, 44(13):  2650-2660 .  doi:10.3864/j.issn.0578-1752.2011.13.003
    Abstract ( 1132 )   PDF (523KB) ( 708 )   Save
    Related Articles | Metrics
      【Objective】The aims of this study is to characterize the distribution of simple sequence repeats (SSR) in the transcribed regions of Panax ginseng, and to develop genomic SSR (G-SSR) and unigene derived microsatellite (UGMS) markers in Panax ginseng. 【Method】A data set of 7 649 expressed sequence tags (ESTs) of P. ginseng downloaded from NCBI was assembled to obtain the unigenes. SSR-containing unigenes and genome were selected to characterize the SSR distribution in ginseng genome. A set of UGMS and G-SSR primers was designed to amplify genomic DNA of ginseng and other Araliaceae species. 【Result】A total of 4 869 unigenes with a length of 2.72 Mb were predicted. From 488 SSR-containing unigenes (10.02%), 724 UGMS were identified with a density of 1 per 3.75 kb of unigenes. Dinucleotide repeats (48.06%) were the most abundant followed by mono (29.28%) and tri-nucleotide repeats (19.06%). The motifs of AT/TA and AAG/CTT were mostly distributed in untranslated region and protein coding region, respectively. Among the 100 UGMS and 44 G-SSR primer pairs, 86 and 44 ones showed amplifications in a set of 9 ginseng accessions. Polymorphisms between the accessions were found to be 42.0% and 43.2%, respectively. The transferability of ginseng UGMS marker to P. guinquefolius, P. notoginseg and E. senticosus was 100%, 87.2% and 75.6%, while 95.5%, 72.7% and 40.9% for G-SSR, respectively. 【Conclusion】The SSR shows higher frequency in ginseng and the most abundant motif is dinucleotide. The distribution of UGMS was non-random with respect to different genic regions. UGMS markers detected a lower polymorphism but a higher level of transferability than those derived from genomic SSR.
    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY
    Effects of Cultivation Methods on Yield, Growth Stage and Utilization of Temperature and Illumination of Rice in Different Ecological Regions
    LI Jie, ZHANG Hong-Cheng, DONG Yang-Yang, NI Xiao-Cheng, YANG Bo, GONG Jin-Long, CHANG Yong, DAI Qi-Gen, HUO Zhong-Yang, XU Ke, WEI Hai-Yan
    Scientia Agricultura Sinica. 2011, 44(13):  2661-2672 .  doi:10.3864/j.issn.0578-1752.2011.13.004
    Abstract ( 1078 )   PDF (348KB) ( 1624 )   Save
    Related Articles | Metrics
    【Objective】This study was conducted to investigate the effects of cultivation methods on yield, growth stage and utilization of temperature and illumination of various types of rice in different ecological regions under the rice-wheat cropping systems. It will provide a theoretical basis for scientific use and division layout of cultivation methods and its supporting variety type.【Method】With medium-maturing late japonica rice, early-maturing late japonica rice, late-maturing medium japonica rice and medium-maturing medium japonica rice (including japonica conventional rice and japonica hybrid rice) as materials, using three cultivation methods of artificial transplanting (AT), mechanical transplanting (MT) and direct seeding (DS), a comparative study of cultivation methods on yield, growth stage and utilization of temperature and illumination of rice was conducted in the following three different ecological regions: Taihu rice district in Changshu (Sunan, 31.4°N), Lixiahe rice district in Jiangyan (Suzhong, 32.3°N) and Xinghua (Suzhong, 32.6°N) and Huaibei rice district in Donghai (Subei, 34.5°N). 【Result】Yield of rice with different cultivation methods was significantly different, and that of AT was the highest, while DS was the lowest. Compared with the AT, the yield of MT and DS reduced by 20-50 kg and 80-180 kg/667 m
    2, respectively. And it demonstrated the law that the reducing extent was greater with latitude increasing. The yield of MT and DS reduced by 20-30 kg and 80-100 kg, 30-40 kg and 90-110 kg, 40-50 kg and 160-180 kg in Sunan, Suzhong and Subei, respectively. In the same region, the reducing extent of late-maturing varieties was greater than that of early-maturing varieties, and conventional rice greater than hybrid rice. The growth process of MT and DS delayed and growth stage shortened than that of AT. The shortening number of days of MT than that of AT was 11-14, and DS was 25-30, and it showed a trend of the higher the latitude the more the shortening number of days. Each growth duration would be shortened, and from sowing to jointing was the most which accounted for over 60% of the total shortening days. For the same type of varieties, the date of jointing, heading and maturity delayed, and the number of days of the growth stage increased with latitude increasing. In the same region, the shortening number of days of late-maturing varieties was more than that of early-maturing varieties. The accumulated temperature and illumination hours of MT and DS significantly reduced, and in particular, the utilization efficiency of accumulated temperature and illumination hours of DS was only 81.8% and 77.4% in Subei. The utilization efficiency of accumulated temperature and illumination hours was different because of variety types in different regions. The utilization efficiency of early-maturing late japonica rice was higher in Sunan, while that of late-maturing medium japonica rice and medium-maturing medium japonica rice in Suzhong and Subei, respectively. 【Conclusion】Cultivation methods had great effects on yield, growth stage and utilization of temperature and illumination of rice. According to the large area yield target of 600 kg/667 m2 in Jiangsu Province, a preliminary division for the suitable range and its supporting suitable variety types of different cultivation methods was made, in order to provide reference for the production of the large area. The preliminary division is that the AT and MT are all suitable in Sunan, Suzhong and Subei, but the DS is suitable in Sunan and sub-suitable in Suzhong while unsuitable in Subei. And each cultivation method has its supporting suitable variety types for different region, respectively.
    Effects of Spraying Exogenous ABA or GA on the Endogenous Hormones Concentration and Filling of Wheat Grains
    YANG Wei-Bing, WANG Zhen-Lin, YIN Yan-Ping, LI Wen-Yang, LI Yong, CHEN Xiao-Guang, WANG Ping, CHEN 二Ying, GUO Jun-Xiang, CAI Tie, NI Ying-Li
    Scientia Agricultura Sinica. 2011, 44(13):  2673-2682 .  doi:10.3864/j.issn.0578-1752.2011.13.005
    Abstract ( 1067 )   PDF (816KB) ( 1311 )   Save
    Related Articles | Metrics
    【Objective】The objective of the experiment is to explore the relationship between exogenous ABA and GA to the levels of endogenous hormones in grains and the grain filling, and provide a theoretical basis for increasing grain weight under the regulation of exogenous hormone.【Method】The field experiments were carried out at Taian Experimental Station of Shandong Agricultural University, two cultivars Shannong 1391 and Gaocheng 8901 were planted in the farm, exogenous ABA or GA to the spikes were sprayed at initial grain filling stage, spikes were sampled after anthesis till maturity, and grains were partitioned into two groups, superior grains (the 1st and 2nd grains from the basal part of middle-spikelets ) and inferior grains (the 3rd and 4th grains from the basal part of middle spikelets or the 3rd grains or the 3rd, 4th and 5th grains).Then, taking the grains for the analysis of endogenous hormone levels and grain filling characteristics.【Result】Spraying exogenous ABA or GA enhanced the grain weight. Spraying exogenous ABA enhanced the weight of superior grains. Spraying exogenous GA enhanced the weight of superior and inferior grain. At the same time, spraying exogenous GA enhanced the levels of endogenous GA in grains. Spraying exogenous ABA enhanced the levels of endogenous ABA in grains. Spraying exogenous ABA or GA enhanced the IAA and CTK content at middle and later periods of grains filling. Grain filling characteristics suggest that spraying exogenous ABA or GA extended the grain filling stage. Spraying exogenous ABA enhanced the initial grain filling rate of superior grains. Spraying exogenous GA enhanced the grain filling rate at middle and later periods of grains filling. 【Conclusion】Spraying exogenous ABA or GA regulated the levels of endogenous hormones in grains, aslo the initial grain filling stage and duration of grains filling stage, and then, grain weight was regulated. Spraying exogenous ABA enhanced the grain weight by enhancing the weight of superior grain. Spraying exogenous GA enhanced the grain weight by enhancing the weight of superior and inferior grain.
    PLANT PROTECTION
    Control of Rice Stripe Disease Using RNAi Technology
    ZHANG Yan, FU Hong-Wei, WANG Shuang, LIANG Guo-Hua, YANG Yi-Zhong
    Scientia Agricultura Sinica. 2011, 44(13):  2683-2691 .  doi:10.3864/j.issn.0578-1752.2011.13.006
    Abstract ( 827 )   PDF (598KB) ( 885 )   Save
    Related Articles | Metrics
    【Objective】The objective of this study is to control the incidence and prevalence of rice stripe disease, change the situation of that there is no specific pesticide to inhibit the occurrence of rice stripe disease once rice is infected with RSV. 【Method】Seed soaking and spraying technologies were used to make biological vaccines absorbed by rice plants. Both the detection of RSV-carrying rate and vaccine residue in rice plant and field survey on occurrence rate of rice stripe disease were conducted to determine vaccine control efficiency to the disease. 【Result】Addition of 1.0‰ B penetrant in the vaccine was the optimal concentration for seed soaking and 5.0‰ A penetrant was the optimal concentration for spraying. The detection rate of vaccine was consistent with the degeneration of RSV. The residue time of vaccine in rice plant was more than 30 d after seed soaking and 10-20 d after spraying. The control effects of two methods both exceeded 70%.【Conclusion】Biological vaccine could be a new biological pesticide for the control of rice stripe disease.
    Identification and Control Effects of Two Antagonistic Actinomycetes Against Clubroot
    WANG Jing, HUANG Yun, YAO Jia, LIN Shan, LI Xiao-Lan, QIN Yun
    Scientia Agricultura Sinica. 2011, 44(13):  2692-2700 .  doi:10.3864/j.issn.0578-1752.2011.13.007
    Abstract ( 967 )   PDF (418KB) ( 993 )   Save
    Related Articles | Metrics
    【Objective】The objective of this study is to identify antagonistic strains A316 and A10 obtained from rhizospheric soil of Chinese cabbage, roots of Ormosia yaanensis respectively which have higher inhibition on the resting spore germination of P. brassicae and to develop effective biological control measures against clubroot. 【Method】Strains A316 and A10 were identified by morphological and cultural traits, physio-biochemical characteristics and 16S rDNA sequence analysis. The control efficiency of A316 and A10 against clubroot of Chinese cabbage in a pot experiment and a field plot experiment were evaluated. 【Result】Based on the 16S rDNA sequence analysis, strain A316 was most closely related to Streptomyces griseoruber NBRC 12873 (99.9% similarity). The morphological and cultural traits, and physio-biochemical characteristics of strain A316 conformed with S. griseoruber. While the genetic relationships of strain A10 with many related sequences from GenBank database were closely related. According to morphological and cultural traits, and physio-biochemical characteristics, A10 was affiliated to Griseofuscus of Streptomyces. The control efficiency against clubroot of Chinese cabbage of A316 and A10 were 72.8%, 67.2% in a pot experiment and 68.5%, 56.7% in a field plot experiment. Yield increase per unit area of Chinese cabbage resulted from A316 and A10 were 96.1%, and 64.9%. 【Conclusion】Strain A316 was preliminarily identified as S. griseoruber. Strain A10 was affiliated to Griseofuscus of Streptomyces. A316 and A10 were approved to be promising biocontrol agents for P. brassicae. A316 had better control efficiency against clubroot of Chinese cabbage than A10.
    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Analysis of Water and Nitrogen Use Efficiencies and Their Environmental Impact Under Different Water and Nitrogen Management Practices
    WANG Huan-Yuan, HU Ke-Lin, LI Bao-Guo, JIN Liang
    Scientia Agricultura Sinica. 2011, 44(13):  2701-2710 .  doi:10.3864/j.issn.0578-1752.2011.13.008
    Abstract ( 994 )   PDF (543KB) ( 1005 )   Save
    Related Articles | Metrics
    【Objective】 The objective of this study was to quantify water and nitrogen use efficiencies (WUE and NUE) and their environmental impact under different water and nitrogen management practices, and to provide some suggestions on making optimal management practice. 【Method】 Two kinds of water and nitrogen management practices, farmers’ practice (FP) and reduced input (RI) practice based on real-time measure of soil water content and soil mineral N concentration, were both conducted under winter-wheat and summer-maize rotation system from Oct. 2004 to Sep. 2006 in the North China Plain (NCP). After a Water and Nitrogen Management Model (WNMM) was calibrated and validated, the yields, amounts of drainage, N leaching, WUE and NUE for both treatments were all calculated. 【Result】 The results showed that the difference of the irrigation amounts between the two treatments was small, while the difference of fertilizer application rate was significant, the amounts for RI (540 kg N·hm-2) was only half of that of FP (1 100 kg N·hm-2). The annual average yields under FP and RI practices were 11 579 and 11 748 kg·hm-2, respectively. The WUE for FP and RI were 1.65 and 1.72 kg·m-3, respectively. Their NUE were 15 and 24 kg·kg-1 N, respectively. N leaching and NH3 volatilization were the main pathway of nitrogen loss. Amounts of N leaching were 407 and 104 kg N·hm-2 for FP and RI, respectively, and NH3 volatilization was 282 and 104 kg N·hm-2 for FP and RI, respectively. 【Conclusion】The yield, WUE and NUE under RI were all higher than those under FP, and total N loss under RI was significantly lower than that under FP.Therefore, water and nitrogen management by farmers’ practice should be improved to develop sustainable agriculture in the NCP.
    Effect of Vegetation Types on Soil Salt Ions Transfer and Accumulation in Soil Profile
    GUO Quan-恩, WANG Yi-Quan, MA Zhong-Ming, GUO Tian-Wen, CHE Zong-Xian, HUANG Gao-Bao, 南Li-Li
    Scientia Agricultura Sinica. 2011, 44(13):  2711-2720 .  doi:10.3864/j.issn.0578-1752.2011.13.009
    Abstract ( 961 )   PDF (919KB) ( 857 )   Save
    Related Articles | Metrics
    【Objective】The characteristics of soil salt ions in soil profile were investigated under different vegetation types, and the purpose was to provide a theoretical basis for excavate and sustainable use of salinization soil.【Method】The object of study was orchard salinization soils in Qin’an County of Gansu Province. A field experiment was carried out to probe into the effects of vegetation type (barley, maize, apple fruit trees and the bare land) on soil salt and ions.【Result】The results showed that in semiarid salinization area, salt in soil profile of the bare land always was in a state of accumulation, the intensity and depth of salt accumulation was gradually increased with time elapsing. Salt accumulation was restrained in soil profile in barley and maize planting land at the late growth stage. However, there were two peak values on salt accumulation in soil profile in fruit trees planting land. The first peak value was from May to June, salt mainly accumulated in the soil layers of 10-30 cm. The second peak value was in middle August, salt mostly accumulated in the soil layers of 30-50 cm. 【Conclusion】Under the condition of agro-forest vegetation in semiarid, vegetation types affected the periods of salt ions accumulation, the intensity of salt ions accumulation, and the place of salt ions accumulation. There was a significant influence of planting barley, maize and apple fruit trees on the content of salt, Cl- and water soluable Ca2+ in soil profile.
    Aggregate Stability of Purple Soil and Its Impacts on Soil Erosion of Slope Dry Land
    CHEN Zheng-Fa, SHI Dong-Mei, XIE Jun-Qiang, ZHANG Bing
    Scientia Agricultura Sinica. 2011, 44(13):  2721-2729 .  doi:10.3864/j.issn.0578-1752.2011.13.010
    Abstract ( 1094 )   PDF (430KB) ( 924 )   Save
    Related Articles | Metrics
    【Objective】The objective of this research was to study the purple soil aggregate stability compared with soil erosion in different land use types of dry land.【Method】By using the methods of Simulated Rainfall and analysis characteristics of soil aggregates, the purple soil aggregate stability characteristics of four land use types and their impacts on the process of erosion were studied.【Result】It was found that fast wetting made the majority of aggregate breaking down to the small aggregates. The great impact of two treatments which were slow wetting and wet stirring was the 5-2 mm large aggregates which the diameter is mainly concentrated in 2-0.5 mm after collapse. The aggregate stability of four land types is mulberry plantation>alfalfa>grassland>vegetable garden. Both the MWD and GMD are the smallest for four land use types of fast wetting, and the largest of the MWD and GMD are slow wetting. The mainly collapse mechanism of soil aggregates in purple soil dry land is fast wetting when the internal air pressure, and the minimal damage is clay swelling. The order of total runoff and sediment in four land use types under simulated rainfall is vegetable land>grassland>alfalfa>mulberry plantation, and the process of runoff and sediment coupled with the aggregate characteristics of stability. The correlation of soil aggregate stability index between runoff rate and sediment yield rate of rainfall is high in the first 1 hour.
    MWD values under fast wetting were significantly negatively correlated with runoff and sediment erosion. The mainly soil erosion of aggregate crushing mechanism of purple soil in dry land is soil aggregate destruction of the internal air pressure.【Conclusion】The worse the stability of soil aggregates, the more erosion and runoff in purple soil dry land. The relation between soil aggregate stability and sediment characteristics is the highest in antecedent rainfall erosion. It is better to use MWD to reflect the relationship between purple soil aggregates stability and erosion and runoff. The mainly soil erosion of aggregate crushing mechanism of purple soil in dry land is soil aggregate destruction of the internal air pressure.
    HORTICULTURE
    Effect of Melon Interstock on the Growth and Fruit Quality of Grafted Cucumber
    ZHANG Yu, LIU Shi-Qi, ZHANG Zi-Kun, YANG Xiao-Jian, MA Lin, HUANG Zhi-Jun
    Scientia Agricultura Sinica. 2011, 44(13):  2730-2737 .  doi:10.3864/j.issn.0578-1752.2011.13.011
    Abstract ( 737 )   PDF (322KB) ( 884 )   Save
    Related Articles | Metrics
    Objective】An experiment was conducted to investigate the effect of melon interstock on the plant growth and quality of greenhouse cucumber fruits.【Method】Comparisons were made on the plant growth, bleeding sap and fruits quality among the self-rooted cucumber, the traditional grafted cucumber and the grafted cucumber with interstock of ‘Elizabeth’ melon, which the scions were ‘Zhongnong No.14’ and the rootstocks were ‘Zhenyoumei’ pumpkin.【Result】Grafted cucumber with melon interstock grew better than self-rooted cucumber, and no significant difference was observed in comparison with traditional grafted ones. The contents of soluble sugar, soluble solids and VC in the interstock grafting were significantly higher than those in traditional grafted treatment, whereas the titratable acid content was obviously lower. Keeping two true leaves of the interstock of melon significantly increased the contents of soluble proteins and dry matter.【Conclusion】Grafting with melon interstock significantly improved the quality of cucumber fruits as well as better growth.
    Changes of the Contents of Functional Substances Including Lycopene, Citrulline and Ascorbic Acid During Watermelon Fruits Development
    WAN Xue-Shan, LIU Wen-Ge, YAN Zhi-Hong, ZHAO Sheng-Jie, HE Nan, LIU Peng, DAI Jun-Wei
    Scientia Agricultura Sinica. 2011, 44(13):  2738-2747 .  doi:10.3864/j.issn.0578-1752.2011.13.012
    Abstract ( 975 )   PDF (484KB) ( 1327 )   Save
    Related Articles | Metrics
    【Objective】The changes of the contents of the functional substances including lycopene, citrulline and ascorbic acid (VC) of watermelon during fruit development were studied in order to provide a theoretical basis for optimization of watermelon breeding. 【Method】The contents of lycopene, citrulline and ascorbic acid (VC) of watermelon during fruit development were analyzed. The lycopene, citrulline and total ascorbic acid (TAA) contents were determined by spectrophotometry methods and ascorbic acid (AA) was determined by 2,6 - dichlorophenolindophenol titration. 【Result】The lycopene accumulation during fruit development of red flesh watermelon fruit was a S-type curve. The lycopene content of young fruit was low, and slow accumulation during fruit development, then lycopene content increased dramatically with the red flesh coloring and achieved the highest value at ripening stage. Decreasing tendency in lycopene contents was observed during overripening. The citrulline contents of young fruits were lower, then it accumulated slowly during fruit development and decreased in fruit enlargement. Then it increased rapidity and achieved the peak value before maturity. The decreasing tendency in citrulline content was noticed in maturity phase. The change of VC contents in different color flesh of watermelons were showed a M-curve shape during fruit development: The content of total ascorbic acid (TAA) in young fruit was high, then reached the first peak of TAA content with the enlargement of the fruit, The TAA contents decreased rapidly to the lowest level and then increased rapidly to achieve the second peak at ripening stage. The decreasing tendency in content was noticed during overripening. Both ascorbic acid (AA) and dehydroascorbic acid (DHAA) contents showed the same change. When achieved the peak, the ratio of TAA﹕AA contents in different fruits of watermelon varieties were about 1-2﹕1. 【Conclusion】The contents of lycopene and V
    C in ripe fruits of watermelon achieved the peak value and the contents of citrilline in nearly mature of watermelon fruit achieved the peak value.
    Isolation of Effective Genes with Digital Northern Platform of NCBI Grapevine (Vitis vinifera L.) EST Sequences
    SHANG Guan-Ling-Fei, WANG Chen, FANG Jing-Gui, LI Xiao-Ying, WANG Xi-Cheng, SONG Chang-Nian
    Scientia Agricultura Sinica. 2011, 44(13):  2748-2759 .  doi:10.3864/j.issn.0578-1752.2011.13.013
    Abstract ( 991 )   PDF (684KB) ( 1000 )   Save
    Related Articles | Metrics
    【Objective】The objective of the study is to take full advantage of mass grapevine (Vitis vinifera L.) expression sequence tags (ESTs) from NCBI database, establish localization digital platform for grapevine genes, and achieve the purpose of rapid large-scale gene expression analysis.【Method】A total of 362 193 grapevine ESTs were downloaded from GenBank, processed further by bioinformatics methods, and used to build the digital Northern platform. The verification of the accuracy of the electronic analysis was done by quantitative RT-PCR technique.【Result】The digital northern platform for eleven tissues and those under five stress conditions were effectively established. The comparison of the results from qRT-PCR of four grape genes (VvAG, VvCHS, VvF3H, VvLDOX) and the corresponding electronic expression analysis data indicated that the predictive efficiency of this platform was high. The efficiency of expression prediction in the organs and tissues (berry, inflorescense, and flower) with more ESTs from them available was good, and the prediction in the tissues which has less ESTs deposited needs more experimental analysis. The predictive result will be closer to the true gene expression situation with more ESTs from various tissues are sequenced and deposited in the NCBI database.【Conclusion】The grape gene digital Northern platform has relatively high efficiency of predicting result, and can provide a useful analysis tool for rapid large-scale analysis of the grapevine gene expression and mining of the related important data.
    STORAGE·FRESH-KEEPING·PROCESSING
    Comparison of Saccharification Effects Between Waxy Wheat and Common Wheat
    LI Bin, XU Zhi-Bin, FENG Bo, WANG Tao
    Scientia Agricultura Sinica. 2011, 44(13):  2760-2767 .  doi:10.3864/j.issn.0578-1752.2011.13.014
    Abstract ( 962 )   PDF (389KB) ( 1313 )   Save
    Related Articles | Metrics
      【Objective】The objectives of this study are to analyze the dynamic changes of main chemical composition of waxy wheat during saccharification, and to determine the optimizing condition of saccharification.【Method】Waxy wheat and common wheat were used as materials for saccharification by inoculating fuqu, then the dynamic changes of main chemical composition was investigated. Meanwhile, fermentable sugars of steamed and saccharified mash were analyzed. Optimal condition of saccharification using waxy wheat as the material was also investigated by orthogonal experiment.【Result】The trend of dynamic change of waxy wheat and common wheat during saccharification was similar. The amount of reducing sugar increased at the beginning and then decreased, reaching maximum at 24 h. Also, the activity of glucoamylase increased at first and reached maximum at 32 h and then decreased. In addition, pH decreased at first and then stayed with little decreasing. The change of total acid was just opposite to pH. Meanwhile, hydroxylation of waxy wheat starch was quicker than that of common wheat, and the reducing sugar content of waxy wheat was higher than that of common wheat when saccharification finished. The activity of waxy wheat glucoamylase during saccharification was slightly lower than that of common wheat. The analysis of fermentable sugars by HPLC showed that the proportion of fermentable sugars to polysaccharide was increased and polysaccharides were hydrolyzed into low molecular and short chain polysaccharides. The optimal condition of saccharification was as follows: saccharification temperature 35℃, fuqu 1%, saccharification time 36 h.【Conclusion】The hydroxylation of waxy wheat starch was quicker than that of common wheat, and reducing sugar content of waxy wheat was higher when saccharification finished. These suggested that saccharification effect of waxy wheat was better than that of common wheat.
    A 90-Day Toxicology Study of Transgenic Rice Expressing Lysine-Rich Protein Fusion Gene in Sprague-Dawley Rats
    DONG Ying, SHI Wei-Dong, ZHOU Xing-Hua, ZHANG Yi, WANG Yun, XIAO Xiang
    Scientia Agricultura Sinica. 2011, 44(13):  2768-2776 .  doi:10.3864/j.issn.0578-1752.2011.13.015
    Abstract ( 850 )   PDF (371KB) ( 563 )   Save
    Related Articles | Metrics
    【Objective】Rats were fed by transgenic rice expressing lysine-rich protein fusion gene to study if the transgenic rice possesses potential toxic or adverse effects.【Method】According to the composition of the transgenic GL gene rice and its parental rice, a nutritionally balanced purified diet with 70% transgenic GL gene rice or 70% parental rice was prepared. And the nutrition of the diet was the same as the control diet. Sixty weanling Sprague-Dawley rats were divided into three groups according to their sex and weight respectively: transgenic rice group, nontransgenic rice group, and control group. They were fed with corresponding diet for 90 days. Indicators including body weight, feed volume, feed utilization rate, blood routine test, blood biochemistry test, urinary routine test, relative organ weights and organ histopathological examinations were observed. 【Result】No significant differences were found between transgenic rice group and non-transgenic rice group (P>0.05) and no histopathological damage was detected. Female feed volume and CHOL of male serum of terminal experiment in transgenic rice group were lower than those of control group (P<0.05). Female feed utilization rate, MCV of male whole blood of terminal experiment, AST of male serum of midterm experiment, AST/ALT and P of male serum of terminal experiment were higher than those of control group (P<0.05), but biological differences were not observed, and the values of all the blood routine parameters and serum parameters were still within the normal ranges of values for rats of this age and sex.【Conclusion】Transgenic rice has no adverse effects on rats, and the transgenic rice is as safe and wholesome as its parental rice.
    ANIMAL SCIENCE·RESOURCE INSECT
    Effects of Dietary Selenium Supplementation on Growth Performance and Melanin Content in Tissues of Taihe Silky Fowls
    LI Guan-Hong, XU Hai-Yan, XU Lan-Jiao, QU Ming-Ren, YOU Jin-Ming, YI Zhong-Hua, PAN Ke
    Scientia Agricultura Sinica. 2011, 44(13):  2777-2786 .  doi:10.3864/j.issn.0578-1752.2011.13.016
    Abstract ( 1203 )   PDF (351KB) ( 702 )   Save
    Related Articles | Metrics
    【Objective】The objective of this study was to investigate the effects of dietary selenium (Se) supplementation on the growth performance and melanin content in tissues of Taihe Silky Fowls and to preliminarily explore the mechanism underlying the effects of selenium on melanin biosynthesis.【Method】A total of 450 one-day old Taihe Silky Fowls were randomly allotted into 6 groups with 5 replicates of 15 birds each. Each group was fed the basal diet supplemented with 0.00, 0.10, 0.30, 0.50, 0.70 or 0.90 mg·kg-1 Se to 4 weeks of age, respectively. At the end of 4 weeks of age, average daily gain (ADG) and feed to gain ratio (F/G) were calculated, and glutathione peroxidase (GSH-Px) activity, tyrosinase (TYR) activity, melanin content in tissues were determined.【Result】Se supplementation in the basal diet significantly improved the growth performance of Taihe Silky Fowls (P<0.05), and the maximum performance was obtained when Se supplementation level was 0.30 mg·kg-1. GSH-Px and TYR activities in serum, liver, kidney, pectoral muscle and skin were significantly improved and the melanin contents in these tissues were also increased (P<0.05) when the diets were supplemented with 0.10-0.70 mg·kg-1 Se. Remarkable quadratic curve relationship between Se supplemental levels and ADG, F/G, GSH-Px, TYR activities and melanin content in tissues was observed (P<0.05). Melanin content in tissues was positively correlated with GSH-Px and TYR activity (P<0.05), respectively. The optimal Se supplementation level for obtaining maximum melanin content in tissues was higher than for obtaining maximum ADG.【Conclusion】Dietary supplementation with Se could improve growth performance and stimulate melanin biosynthesis of Taihe Silky Fowls, and the optimum Se requirement for melanin deposition in tissues was higher than for maximal growth. The stimulating effect of selenium on melanin synthesis may be related to the increased antioxidant capacity of body exerted by Se.
    Cloning and Expression Pattern of Protein Kinase B/AKT Gene in Inner Mongolia Cashmere Goat
    YANG Jiao-Fu, SHI Ji-Jun, LIANG Yan, ZHENG Xu, ZHANG Tao, QIN Yi, WANG Zhi-Gang, LIU Dong-Jun
    Scientia Agricultura Sinica. 2011, 44(13):  2787-2795 .  doi:10.3864/j.issn.0578-1752.2011.13.017
    Abstract ( 856 )   PDF (640KB) ( 877 )   Save
    Related Articles | Metrics
      【Objective】The present study aims at cloning the CDS fragment of AKT gene cDNA in Inner Mongolia Cashmere Goat and analyzing its tissue-specific expression. 【Method】AKT gene cDNA was cloned by RT-PCR. The nucleotide sequence was analyzed by BLAST and amino acid sequence was analyzed by online softwares SMART and Psite. The tissue-specific expression pattern of AKT was analyzed by semi-quantitative RT-PCR. The expression of AKT in goat fibroblasts was detected by Western blotting.【Result】The cloned AKT gene cDNA was 1 443 bp in length, including a complete ORF encoding 480 amino acids. The amino acid sequence shares 97% identity with the sheep AKT (NM_001161857.1). Analysis by SMART suggested that the encoded protein contained a PH domain which can bind 3-phosphoinositides and an S_TKc domain which possess serine/threonine kinase catalytic activity. Analysis with Psite indicated one cAMP-/cGMP-dependent protein kinase phosphorylation site, 6 protein kinase C phosphorylation sites, 10 casein kinase II phosphorylation sites, 2 protein kinases ATP-binding region signatures and one serine/threonine protein kinases active-site signature in this protein. Analysis by Psort suggested that this protein most probably localizes in cytoplasm. The expression of AKT mRNA was higher in testicle, brain and kidney, while lower in spleen, liver, lung and mammary glands. The expression of AKT in goat fibroblasts was inhibited when the cells were treated by mTOR specific inhibitor CCI-779 for 48 hours. 【Conclusion】The full length of AKT gene ORF in Inner Mongolia Cashmere Goat was cloned and shared high identity with sheep. AKT gene was shown to express in testicle, brain, kidney, spleen, liver, lung and mammary glands. The expression of AKT in goat fibroblasts was regulated by mTOR signaling pathway.
    Construction of Molecular Genealogy and Analysis of Family Genetic Characteristics in Laoshan Dairy Goat
    JI Zhi-Bin, WANG Gui-Zhi, WANG Jin-Feng, CHEN Shan-Shan, WANG Yong, ZHAO Jin-Shan, CHENG Ming, WANG Jian-Min
    Scientia Agricultura Sinica. 2011, 44(13):  2796-2807 .  doi:10.3864/j.issn.0578-1752.2011.13.018
    Abstract ( 985 )   PDF (845KB) ( 863 )   Save
    Related Articles | Metrics
    【Objective】The molecular genealogy of population known pedigree records in Laoshan Dairy Goat was constructed by using microsatellite DNA markers technology to verify the consistency with the known pedigree records, identify the factors affecting paternity testing, ascertain the genetic characteristics of family and provide a scientific basis for the conservation and genetic management of the groups. 【Method】Twelve microsatellite markers with high polymorphism were selected from 25 ones for 212 Laoshan Dairy Goat with known pedigree records, parentage were analyzed with Cervus vs 2.0, molecular genealogy was built with Pedigraph vs 2.2, the genetic characteristics and phylogenesis of family were analysed with Molkin vs 3.0, MEGA4 and GeneClass2. The correlation coefficient between the exclusion probability(EP) per locus and PIC, He, Ho, K was analyzed with SPSS vs 16, and the effect of different locus and information source on the accuracy of paternity testing was analysed, also.【Result】Twelve highly polymorphic microsatellite DNA markers were selected from 25 ones in Laoshan Dairy Goat, the mean PIC value is 0.687, the mean number of allele is 5.75, the mean expected heterozygosity is 0.730. The two-parents combined exclusion probability is 0.9998 on the confidence level of 95%. The biological mother or grandmother of 115 offsprings were found from 80 candidate parents, 3 individuals were not accorded with the known pedigree records, the conincidence rate is 98.58%. The molecular genealogy was constructed on the basis of paternal strains. The correlation coefficient between the exclusion probability and the PIC is the biggest (0.99), and K with the smallest correlation coefficient (0.67). The CEP2 is more than 99.73% with 8 microsatellite loci. 【Conclusion】Twelve microsatellite loci can be used for analysis of paternity or construct molecular genealogy in Laoshan Dairy Goat group. The built families have a good genetic basis, and a high genetic diversity within each family or among families.
    Analysis T>C Polymorphisms Located in the Seed Regions of Mir-1644 Gene of Chicken
    GENG Li-Ying, ZHANG Chuan-Sheng, YIN Chun-Guang, CAO Ding-Guo, DU Li-Xin, CHEN Juan
    Scientia Agricultura Sinica. 2011, 44(13):  2808-2813 .  doi:10.3864/j.issn.0578-1752.2011.13.019
    Abstract ( 735 )   PDF (343KB) ( 569 )   Save
    Related Articles | Metrics
    【Objective】This experiment was conducted to study the effect of T>C polymorphisms located in the seed regions of mir-1644 on its stem-loop secondary structure and target selection, and the differences of distribution of this polymorphisms were also detected in different chicken breeds.【Method】The secondary structures of different alleles of mir-1644 and their target genes were predicted using mfold and miRanda software respectively. CRS-RFLP technique was used to detected chicken mir-1644 genotype in 6 populations containing 179 individuals.【Result】The results showed that (1) The T→C mutation results in pre-mir-1644 secondary structure change and the free energy of pre-mir-1644-C is higher than that of pre-mir-1644-T; (2) There were 21 target gene differentially regulated by mir-1644-C and mir-1644-T were predicted; (3) Allele C was the dominant allele in Wenshanluhua chicken population, while allele T was the dominant allele in Jiningbairi, Langya, Wugu and populations. The allele and genotype distribution in Wenshangluhua chicken was significantly different with that in other chicken populations (P<0.01).【Conclusion】These results indicated that T→C mutation may destroy and alter expression and regulation of mir-1644, and CC genotype may be related to some phenotypic variation of Wenshangluhua Chicken.
    Analysis of Responsive Genes in the Midgut of Silkworm Infected with Bombyx mori Cytoplasmic Polyhedrosis Virus
    WU Ping, LIU Ting, QIN Guang-Xing, GUO Xi-Jie
    Scientia Agricultura Sinica. 2011, 44(13):  2814-2822 .  doi:10.3864/j.issn.0578-1752.2011.13.020
    Abstract ( 876 )   PDF (522KB) ( 723 )   Save
    Related Articles | Metrics
    【Objective】The objective of this study is to screen the responsive gene of silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) and establish a foundation for elaborating the molecular mechanism of BmCPV infection.【Method】A microarray system comprising about 23 000 oligonucluotide probes was employed to compare differentially expressed genes in the midguts of BmCPV-infected and normal silkworm larvae at 24, 48 and 72 h post-inoculation. Functions, classifications and pathways of these differentially expressed genes were analyzed by bioinformation tools. Seventeen differentially expressed genes were identified by quantitative real-time PCR. 【Result】At 24, 48 and 72 h post-inoculation, 9, 51 and 258 differentially expressed genes were obtained, respectively. KEGG pathways analysis indicated that at 48 and 72 h post-inoculation, most genes related to metabolism pathways were down-regulated while expressions of genes involved in ribosome and proteasome pathway were all up-regulated. The expressions of several immune-related genes including serpin5, lipase, heat shock proteins, cytochrome P450s and ribosomal P0 protein were up-regulated. 【Conclusion】Differentially expressed genes identified may be responsive to the BmCPV infection. The results provided new clues for investigating the molecular mechanism of BmCPV infection.
    VETERINARY SCIENCE
    Key Proteins Involving in the Assembly and Release of Influenza A Virus-Like Particles (VLPs)
    ZHANG Shu-Mei, HUANG Hai-Bi, YAN Pi-Xi, TENG Qiao-Yang, YAN Li-Ping, XU Da-Wei, HU He-Ba-Te-尔, LI Ze-Jun
    Scientia Agricultura Sinica. 2011, 44(13):  2823-2829 .  doi:10.3864/j.issn.0578-1752.2011.13.021
    Abstract ( 715 )   PDF (673KB) ( 943 )   Save
    Related Articles | Metrics
    【Objective】Virus-like particles (VLPs ) play a crucial role in research of virology and development of novel vaccines. In this study the authors focus on the major structural proteins which involves in the assembly and release of influenza A VLPs.【Method】A serial of eukaryotic expression plasmids, including the plasmid simultaneously expressing HA, NA and M1 proteins, the plasmid simultaneously expressing HA and NA proteins and the plasmid expressing HA protein of influenza virus, were developed. To determine the key proteins in assembly and release of VLPs, 293T cells were transfected with the plasmids mentioned above, and VLPs were detected by hemagglutinin test and transmission electron microscope observation. 【Result】The result showed that the 293T cells simultaneously expressing HA, NA and M1 proteins, or HA and NA proteins could produce VLPs, while the 293T cells expressing only HA could not. 【Conclusion】In this study, the eukaryotic expression plasmid which could express 3 proteins simultaneously was used to determine the key proteins in assembly and release of VLPs. The results indicated that both HA and NA were crucial in this process, while M1 was not necessary.
    Bacteriostatic Activity of Bacteriocin Subtilin112 Against Staphylococcus aureus CVCC 1885 in Vitro and in Vivo
    DENG Kai, SU Li-Fang, ZHANG Ri
    Scientia Agricultura Sinica. 2011, 44(13):  2830-2837 .  doi:10.3864/j.issn.0578-1752.2011.13.022
    Abstract ( 1035 )   PDF (515KB) ( 1087 )   Save
    Related Articles | Metrics
    【Objective】 In order to provide the theoretical basis for clinical application of the bacteriocin Subticin112 secreted by Bacillus subtilis, a research was conducted on bacteriostatic activity of bacteriocin Subticin112 against Staphylococcus aureus CVCC 1885 in vitro and in vivo. 【Method】 Bacteriostasis in vitro: Subticin112, oxytetracycline, penicillin, vancomycin, neomycin, strepomycin and gentamicin in the same effective weight were dissolved into equal volume of sterile saline, respectively. Then the agar diffusion method was used to compare the bacteriostatic activity of the medicines against S. aureus. Bacteriostasis in vivo: 120 SPF-class KM male mice (weighted from 18 g to 22 g) were divided into six groups, they were blank control group, negative control group, oxytetracycline treating group and three Subticin112 treating groups. After the beginning of the experiment, every mouse in each group was orallly administrated 0.4 mL different concentrations of medicine or sterile saline every other 8 hours, after 3 times of oral administration, the mice in each group were intraperitoneal injected at the absolute lethal dose of S. aureus or sterile saline. At the end of the experiment, the mortality was calculated, the proinflammatory cytokines and the cfu values of S. aureus in the peritoneum of the mice were determined, and then the histopathological changes of the tissues were observed. 【Result】Bacteriostasis in vitro: Subticin112 expressed a fine bacteriostatic activity compared to the same concentration of other medicines. The bacteriostatic activity of Subticin112 was highly significantly better than that of vancomycin and neomycin (P<0.01), but differed insignificantly from other antibiotics (P>0.05). Bacteriostasis in vivo: after the experiment, the mortality in negative control group was 100%, while which was 30% in low-dose Subticin112 treating group, and other groups did not have dead mice. The medicine treating groups showed a low concentration of the proinflammatory cytokines (P<0.05), also had a low cfu values compared to the negative control group (P<0.01) and the dose-respones relationship was clear. The histopathological observations showed that the liver and spleen of the mice in the negative control group had obvious pathological lesions, whereas the pathological changes in the medicine treating groups was slight or not distinct. 【Conclusion】These studies provided evidences that Subticin112 have a fine bacteriostatic activity against S. aureus CVCC 1885 in vitro and vivo, and can be developed as a new type of antibacterial agent.
    AGRICULTURAL ECONOMY & MANAGMENT
    A Food Security Assessment in APEC Based on Grain Productivity
    YU Qiang-Yi, WU Wen-Bin, TANG Hua-Jun, CHEN You-Qi, YANG Peng
    Scientia Agricultura Sinica. 2011, 44(13):  2838-2848 .  doi:10.3864/j.issn.0578-1752.2011.13.023
    Abstract ( 1163 )   PDF (517KB) ( 1323 )   Save
    Related Articles | Metrics
    【Objective】The APEC (Asia-Pacific Economic Cooperation), due to its great typicality and importance in world food system, was selected to conduct food security assessment among its economies in this study. 【Method】Intended to highlight grain production in food system, this study firstly used the mechanism method to calculate the productivity potential of radiation, thermal, climatic, land and grain production-potential in APEC region regressively, which was taken as the basis of food security assessment. And then some important indicators were selected to build the index system to assess the food security level in each APEC member economies by using the Analytic Hierarchy Process (AHP). 【Result】The results show that the overall grain production potential of APEC region reaches as high as 1.7 billion tons. Compared with the real grain production of 1.1 billion tons, there is a possibility for all the economies to increase their production. While concerning population carrying capacity and food consumption, food security level in each economy varies greatly, and developed economies are in general more secured than developing economies, except for Japan and Korea. 【Conclusion】Exploring grain production-potential at uttermost is a reasonable way to improve food security, at the same time, it is necessary to control population explosion, especially for those overloaded regions.