Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (10): 2081-2093.doi: 10.3864/j.issn.0578-1752.2011.10.013

• HORTICULTURE • Previous Articles     Next Articles

Molecular ID Establishment of Main China Peach Varieties and Peach Related Species

 CHEN  Chang-Wen, CAO  Ke, WANG  Li-Rong, ZHU  Geng-Rui, FANG  Wei-Chao   

  1. 1.中国农业科学院郑州果树研究所，郑州450009
  • Received:2010-10-15 Online:2011-05-15 Published:2011-01-10

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Abstract: 【Objective】 A total of 237 peach germplasms including China landraces, bred varieties, and related species selected from national grape and peach repository of Zhengzhou were studied with SSR(simple sequence repeat) markers. Analysis was made to establish the 237 peach germplasms molecular ID and to study the methods for distinguishing the germplasms more simply.【Method】Germplasms were distinguished with selected SSR markers, bands that amplified by each marker were coded, then combined the code as a molecular ID. 【Result】The results showed that after screening of the 80 primers, 203 alleles were detected using 16 selected SSR markers located on each chromosome of peach, with a mean value of 12.7 alleles/locus. There were 123 selected alleles were coded to establish germplasms molecular ID based on strategy that alleles located both in landraces, bred and related germplasms. 【Conclusion】There were 202 out of 237 germplasms could be identified with an particular combined code numbers. Moreover, the identifying efficiency of different combinations of the primers were analyzed, 176 germplasms could be identified using 8 reduced core primers, with an average value of 22.1 germplasms/primers. The remaining primers have a higher identifying efficiency and more germplasms could be identified separately. At last, for distinguish germplasms more simply, through selection of the primer pairs according to its PIC step by step, the germplasms could be distinguished by a practical primer choice.

Key words: peaches (Prunus persica (L.) Batsch), related species, SSR, molecular ID

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