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Genetic Variation in CAPN1 Gene and Its Association with Carcass Traits in Chinese Cattle

WU Xiu-xiang; SHI Xue-kui; WU Hai-tao; MAO Yong-jiang; YANG Zhang-ping;LI Jun-ya; GAO Hui-jiang   

  1. 1、College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu;
    2、Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193
  • Received:2010-06-25 Online:2011-04-02 Published:2010-12-31

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Abstract: 【Objective】To explore the distribution of Calpain 1 gene (CAPN1) in Chinese south yellow cattle populations and north beef cattle populations, and to evaluate it as a candidate molecular marker associated with beef quality. 【Method】PCR-SSCP method was used to detect the polymorphism of CAPN1 in 367 heads from four cattle populations, including Leiqiong, Yunnan highpump, BMY and Chinese Simmental. The association between SNP locus and some meat straits were analyzed by general linear model (GLM) in SPSS program. 【Result】 Three genotypes, AA, AG, GG and CC, CT, TT, were detected at site A4558G and C4684T, respectively, with G as an advantageous gene at site A4558G, and A as an advantageous gene at site C4684T. Genotypic variance had significant association with shearing force strait and marbling score strait. The cattle with GG in site A4558G had lower shearing force and marbling score than others (P＜0.05), while the cattle with TT in site C4684T had lower shearing force than that of CT (P＜0.05) and individuals with TT had lower former PH after rowing of acid than that of CC (P＜0.05). Combined-genotype analysis indicated that the cattle with GGTT had lower shearing force than that of AACC, AGCT and AACT (P＜0.05) and lower score of marbling than that of the rest (P＜0.05), while the score of fat color of these cattle were higher than others. But there were no significant associations between two sites and other straits. 【Conclusion】 Site C4684T and A4558G of CAPN1 gene could be used as genetic marks for beef tenderness and marbling score traits.

Key words: CAPN1 gene , analysis of population genetic variations , carcass traits , SNP

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