Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (2): 411-416 .doi: 10.3864/j.issn.0578-1752.2010.02.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Sequencing and Analysis of Complete Genome of a Porcine Circovirus Type 2-like Agent P1

WEN Li-bin, HE Kong-wang, YANG Han-chun   

  1. （江苏省农业科学院兽医所）
  • Received:2008-04-16 Revised:2009-09-17 Online:2010-01-20 Published:2010-01-20
  • Contact: HE Kong-wang, YANG Han-chun

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Abstract:

【Objective】 The aim of the experiment is to discover an agent containing high homologous sequences of porcine circovirus type 2 in swine. 【Method】 PCR was developed for detection of DNA extracted from porcine sera collected from pigs with clinical signs of postweaning multisystemic wasting syndrome, then a reverse PCR was carried out based on the acquired sequences in this study, the sequences of PCR fragments were assembled into consecutive sequence and then finally verified by amplifing the agent complete genome. 【Result】 Complete nucleotide sequence of genome of porcine circovirus type 2-like agent P1 was firstly reported in this paper. P1 contained circular genomic DNAs of 648 nucleotides and three open reading frames. Except 16 nucleotides in the 5′ end of the genome, the genome of P1 showed 98.42% of nucleotide homology compared with porcine circovirus type 2 BF isolate. Phylogenetic analysis revealed that P1 was closely related to the porcine circovirus. 【Conclusion】 Results suggested that there existed porcine circovirus type 2-like agent P1 in swine.

Key words: porcine circovirus type 2, P1, genome, sequencing and analysis

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