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    20 January 2010, Volume 43 Issue 2
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Study on Morphological Structure and Genetic Mapping of Two Novel Leaf Color Mutants in Rice

    ZHANG Li-ke,LI Zhi-bin,LIU Hai-yan,LI Ru-hai,CHEN Man-yuan,CHEN Ai-guo,QIAN Yi-liang,HUA Ze-tian,GAO Yong-ming,ZHU Ling-hua,LI Zhi-kang

    Scientia Agricultura Sinica. 2010, 43(2):  223-229 .  doi:10.3864/j.issn.0578-1752.2010.02.001
    Abstract ( 1059 )   PDF (729KB) ( 1446 )   Save
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    【Objective】 The objective of this study is to analyze the morphological structure, chlorophyll components, chloroplast ultrastruture and map genes underlying the two novel leaf color mutants in rice. 【Method】 A white stripe leaf mutant and a yellow leaf mutant were found in rice breeding materials. The main agronomic character and chloroplast ultrastructure of the mutants were observed. The mutant genes was mapped with SSR markers and F2 mapping population of the cross between the two mutant lines. 【Result】 Compared to their wild-type, the panicles per plant of the white stripe leaf mutant decreased by 12.86%, and the growth duration increased by 11.27%; the plant height of yellow leaf mutant decreased by 31.08% and the growth duration increased by 17.86%, and the photosynthetic pigment contents of both mutants were significantly lower than their wild-type. The result of electron microscope observation showed that the grana structures were unnormal in both mutants. The genetic analysis indicated that the white stripe leaf mutant was controlled by a recessive nuclear gene located on the tip of the short arm of rice chromosome, 9.6 cM from marker RM1331, named st9(t)(stripe) here, and the yellow leaf mutant was controlled by another recessive nuclear gene, mapped on the short arm of rice chromosome 3, within the interval between markers RM411 (1.2 cM) and RM8202 (5.1 cM) named chl12(t) (Chlorophyll-deficit). 【Conclusion】 The two novel mutant genes were mapped, which laid a good foundation for fine mapping, cloning and functional analysis of the mutant genes.
    Genetic Effects and Heterosis Analysis on Yield-related Traits of CMS-FA Hybrid Rice
    WANG Hong-fei,WANG Nai-yuan,LI Yu,LIANG Kang-jing,QIU Xiu-li,ZHOU Wei-ying
    Scientia Agricultura Sinica. 2010, 43(2):  230-239 .  doi:10.3864/j.issn.0578-1752.2010.02.002
    Abstract ( 1001 )   PDF (267KB) ( 964 )   Save
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    【Objective】 This study aimed at detecting the genetic effects and heterosis level of yield-related traits of CMS-FA sterile lines and restorers as well as the breeding potential of CMS-FA hybrid rice parents. 【Method】 By incomplete diallel cross design, using five CMS-FA sterile lines as female parents and five corresponding restorers as male parents, twenty-five crosses were made. The parents and F1 generations were planted to obtain the data of yield-related traits. By using the additive-dominant model and MINQUE(1) method, the genetic variance, heritability, parent additional effects and combination dominant effects, and heterosis of ten yield-related traits were analyzed. 【Result】 Yield-related traits of CMA-FA hybrid rice were controlled by both additive and dominant effects, among which panicle number per plant, total grain number per panicle, filled grains per panicle, seed-set percent, 1000-grain-weight, flag-leaf length and growth duration and so on were mainly controlled by additive effects. The dominant effects prevailed in the traits of yield per plant, plant height, and panicle length and so on. As for narrow heritabilities, the dominant effects of 1000-grain-weight were the highest, the growth duration was the next, and the yield per plant was the lowest. Sterile line Jinnong 2A and restorers Jinhui 3 and Jinhui 5 had the best additive effects. The four combinations with the strongest dominant effects were Jinnong 2A×Jinhui 5, Jinnong 2A×Jinhui 2, Jinnong 4A×Jinhui 5 and Jinnong 5A×Jinhui 5. Three yield-related trais yield per plant, plant height and panicle length had stronger population mean heterosis of 7.351%-16.330% and population over-parent heterosis of 4.233%-10.507% in F1 with the highest heterosis reaching 10.307-49.462%. 【Conclusion】 Jinnong 2A was the best one of all the sterile lines tested, Jinhui 3 and Jinhui 1 were the best ones of restorers tested, and Jinhui 5 was a good parent for the development of high-yielding hybrid rice combination with short growth duration.

    Population Structure and Genetic Diversity of Global Pea (Pisum sativum L.) Germplasm Resources
    ZONG Xu-xiao,GUAN Jian-ping,WANG Hai-fei,MA Yu
    Scientia Agricultura Sinica. 2010, 43(2):  240-251 .  doi:10.3864/j.issn.0578-1752.2010.02.003
    Abstract ( 1010 )   PDF (561KB) ( 2085 )   Save
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    【Objective】 Assessing the genetic diversity between Chinese and alien accessions of cultivated pea (Pisum sativum L.) sampled from National Gene Bank, analyzing their differentiation on allelic loci and population structure, the study try to evaluate the importance and value of these conserved genetic resources, to provide essential information for guidance on strategy and direction choices for future studies of pea genetic resources in China, and for the effective exploration and utilization of global pea genetic resources. 【Method】 1 984 cultivated pea accessions from 66 countries of five continents and 28 provinces of China were employed for SSR analysis using 21 polymorphic primer pairs to detect genetic diversity and population structure. The Structure 2.2 software was used for population structure detection, definition of real population number, genotype allocation to its real population, and calculation of related parameters. Calculation of genetic distance, PCA analyses, 3-dimensional PCA graph, was conducted and drawn by NTSYSpc 2.2d statistical package. Allelic statistics were carried out by Popgene V1.32. The significance test between groups of genotypes was carried out by Fstat 2.9.3.2 statistical package. 【Result】 Based on SSR markers, the observed number of alleles (NA), the effective number of alleles (NE), the ratio of NE/NA, the allelic richness (AR), the gene diversity (GD) and the Shannon's information index (I) of pea germplasm resources from China and foreign countries were thoroughly compared. Except the observed number of alleles (NA), the values of other parameters on genetic diversity detected from Chinese landraces were all higher than that from foreign germplasm resources. Among the 21 tested SSR loci, the difference between Chinese and foreign germplasm collections appeared in 7 SSR loci. The population structure analysis divided all the 1 984 tested genotypes into 3 populations (Pop A, Pop B and Pop C). Pop A consisted of almost all alien accessions (96.49% genotypes of all alien accessions), referred to foreign germplasm population. Pop B consisted of most accessions from Shaanxi and Inner Mongolia (88.18% genotypes of this population), standing for typical Chinese spring sowing germplasm population. Pop C consisted of majority (52.05% genotypes of this population) from Chinese winter sowing areas, and minority (47.44% genotypes of this population) from Chinese spring sowing areas, standing for Chinese winter sowing and spring sowing (except Inner Mongolia and Shaanxi) germplasm population. There were significant differences among the three populations. 3-dimension PCA graph showed 3 concentrated domains with clear boundaries in between, each of the domain (gene pools I, II and II) approximated to Pop A, Pop B and Pop C. 【Conclusion】 The genetic diversity level of domestic Chinese accessions as a group was generally higher than that of alien group, while the level of difference among genotypes within alien group on each parameter was higher than that within Chinese accession group. Three independent populations named Pop A, Pop B and Pop C were detected by population structure analysis, with significant difference in genetic diversity among them. 3-dimension PCA graph showed 3 concentrated domains (gene pools I, II and II) with obvious boundaries in between. Gene pool I concentrated alien genotypes, Gene pool II concentrated Shaanxi and Inner Mongolian genotypes, and Gene pool III concentrated genotypes from Chinese winter sowing areas and spring sowing areas except Shaanxi and Inner Mongolia. By pairs, the three gene pools detected by PCA almost equal to the three populations identified by Structure 2.2 in genotype composition. Pop A approximated to Gene pool I, Pop B approximated to Gene pool II,and Pop C approximated to Gene pool III. Results from PCA fully supported the findings from population structure analysis, within global cultivated pea genetic resources the three gene pools can be defined. Alien genotypes constructed Gene pool I, Chinese genotypes constructed Gene pool II and Gene pool III, which revealed the importance of Chinese and alien collections and Chinese collection was superior.

    Molecular Cloning, Expression Vector Construction and Prokaryotic Expression of BnClo1 Gene from Brassica napus
    DING Yong,CHANG Wei,LIU Xiao-zhu
    Scientia Agricultura Sinica. 2010, 43(2):  252-258 .  doi:10.3864/j.issn.0578-1752.2010.02.004
    Abstract ( 1106 )   PDF (420KB) ( 848 )   Save
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    【Objective】 BnClo1gene cloned from Brassica napus was expressed in Escherichia coli ER2566. 【Method】 A cDNA fragment about 750 bp was amplified from the total RNA of rape seeds by reverse transcription PCR (RT-PCR) with a pair of specific primers based on the sequences of the BnClo1 gene. The recombinant prokaryotic expression vector pTYB12-BnClo1 was constructed by inserting the cDNA fragment encoding the mature peptide of caleosin into the prokaryotic expression vector pTYB12, and then transformed into E. coli ER2566 (DE3). 【Result】 Sequence analysis showed that the fragment length was 768 bp containing a full coding region of 738 bp encoding 245 amino acid residues with a molecular mass of 28.1 kD. The SDS-PAGE electrophoresis analysis showed that the best expression was induced by 20℃ and 4 mmol?L-1 IPTG, under which a relative molecular weight of 83.1 kD recombinant protein intein-caleosin was produced. 【Conclusion】 A BnClo1gene of rape was cloned and optimizing expressed in E. coli. These results should provide a foundation for further purifying and identifying target protein and function study of rape caleosin protein.

    Correlation Analysis of Agronomic Characters and Heritability of Saponins Content in Medicago sativa L.
    GAN Zhi-cai,SHANG Lun-xue,LIU Yong,YU Yong-xiong
    Scientia Agricultura Sinica. 2010, 43(2):  259-265 .  doi:10.3864/j.issn.0578-1752.2010.02.005
    Abstract ( 945 )   PDF (236KB) ( 511 )   Save
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    【Objective】 Through correlation analysis between saponins content and agronomic characters in alfalfa, the authors try to find out one trait which can sign saponins content quickly and explore the rule about heritability and variation of saponins content.【Method】 A new cultivar of Hot-wet alfalfa named Yumu No.1 was used as materials in this research, one hundred alfalfa plants were determined, five high saponin plants and five low saponin plants were selected. 【Result】 Saponins heritability of these 10 alfalfa plants changed from 0.118 to 0.745 through experiments on the lines of offsprings of every plant, and variation coefficient of the lines of offsprings changed from 15.54% to 28.81%, the heritability of high and low saponin groups was 0.636 and 0.208, respectively, and the heritability of high saponin group was obviously higher than low saponin group. The result of correlation and path analysis between the saponins content and plant height, leaf color, pest extent, dry weight as well as the dry matter content showed that there was an extremely significant relationship (r=-0.405**) between leaf color and saponins content, and the saponins content was affected by leaf color directly (P=-0.422**) . The correlation between dry weight and saponins content was affected by plant height, leaf color and dry matter indirectly. 【Conclusion】 It is difficult to breed low saponin cultivar in alfalfa through general breeding method, but some lines of offsprings with high saponin content can be used as materials for breeding of high saponin content alfalfa cultivar. Leaf color of alfalfa can be used as a marker to identify saponins content in alfalfa quickly.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY
    Rule of Grain Yield Components from High Yield to Super High Yield and the Characters of Super-high Yielding Japonica Super Rice
    WU Gui-cheng,ZHANG Hong-cheng,QIAN Yin-fei,LI De-jian,ZHOU You-yan,XU Jun,WU Wen-ge,DAI Qi-gen,HUO Zhong-yang,XU Ke,GAO Hui,XU Zong-jin,QIAN Zong-hua,SUN Ju-ying,ZHAO Pin-heng
    Scientia Agricultura Sinica. 2010, 43(2):  266-276 .  doi:10.3864/j.issn.0578-1752.2010.02.006
    Abstract ( 1075 )   PDF (275KB) ( 1374 )   Save
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    【Objective】 Rice is one of the important food crops in China. The realization of its super-high-yielding has a great significance for food security of the country. This study investigated the rule of grain yield components from high-yield to super-high-yield and the characters of super-high-yielding Japonica super rice. 【Method】 The grain yield and its components and the population sink and its filling of grain yield of three types of populations (High Yield: 8 250-9 750 kg?hm-2; Higher Yield: 9 750-11 250 kg?hm-2; Super High Yield:>11 250 kg?hm-2) in four Japonica super rice (Wujing 15, Huaidao 9, Xudao 3 and Changyou 1) were analyzed. 【Result】 Super-high-yielding rice had more population spikelets than the high-yielding rice and higher-yielding rice (The difference among them was significant). There was no significant difference in filled-grain and 1000-grain- weight among the grain yield of three types of populations. The relationship between the population spikelets and grain yielding was significant at safe maturity. In order to enlarge the population spikelets, it almost depended on enriching panicles from high-yield to higher-yield, and increasing spikelets per panicles was the major factor from higher-yield to super-high-yield. There was no significant difference in filling rate of sink among the grain yield of three types of populations, but the amount of actual filling of sink in super-high-yielding rice was more than the high-yielding rice and higher-yielding rice at safe maturity. 【Conclusion】 The characters of super-high-yielding rice are enriching the amount of actual filling of sink through keep normal filling rate of sink, and thus forming a safe and large sink made up of enough big panicles.

    Study on the Trends in Yield Change of Maize Single Cross Hybrids in Different Periods in China
    FENG Guang,LIU Zhi-fang,LI Yan-yan,JING Xi-qiang,XING Jin-feng,HUANG Chang-ling
    Scientia Agricultura Sinica. 2010, 43(2):  277-285 .  doi:10.3864/j.issn.0578-1752.2010.02.007
    Abstract ( 1043 )   PDF (331KB) ( 1179 )   Save
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    【Objective】 The experiment was conducted to provide a theoretical basis and reference opinions for maize (Zea mays L.) breeding by researching yield, heterosis and main agronomic characters of maize hybrids in different periods in China.【Method】 Twenty maize single cross hybrids and thirty-two parents in different periods utilized widely in China were chosen as materials in the study. The changing trend in yield, heterosis and agronomic characters, correlation analysis between yield and agronomic characters were made by split plot design in three regions. 【Result】 The results showed that the yield of hybrids and mid-parents increased gradually with the varieties replacement. The yield of the earlier varieties showed a decreasing trend in high plant density, while the yield and plant density increased accordantly in present varieties. The heterosis of hybrids presented a trend of up and down in low and high plant density, respectively, and no change was found in middle and average density. Investigation on the changes in plant traits showed that the tassel branch number and the angle between leaf and the uppermost ear reduced gradually, and rows per ear, ear diameter, kernel length, and 100-kernel weight increased gradually, but the kernels per row, shelling percentage and bulk density not changed. The relationship between yield and heterosis was not significant. Rows per ear, ear diameter, kernel length, 100-kernel weight and yield of mid-parents had a significant positive relationship with yield at 0.01 level. The tassel branch number and the angle between leaf and the uppermost ear had a significant negative relationship with yield at 0.01 level. 【Conclusion】 It was found that there was a conflicting evidence to support the heterosis that is the principal contribution to yield increase, the contribution of maize yield increase should owe to the increase of mid-parents yield and plant density, optimization of plant type and ear traits, decrease of tassel branch number.

    Evaluation of Waterlogging Tolerance in Rapeseed (Brassica napus L.) DH Lines at Seedling Stage

    LI Zhen,PU Yuan-yuan,GAO Chang-bin,ZHOU Guang-sheng,TU Jin-xing,FU Ting-dong

    Scientia Agricultura Sinica. 2010, 43(2):  286-292 .  doi:10.3864/j.issn.0578-1752.2010.02.008
    Abstract ( 1261 )   PDF (245KB) ( 846 )   Save
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    【Objective】 The objective of the study was to evaluate waterlogging tolerance of the rapeseed (Brassica napus L.) doubled haploid (DH) population and to screen the most waterlogging-tolerant and waterlogging-sensitive DH lines. 【Method】 For phenotyping, the rapeseed seedlings were grown in plastic pots. Six traits (plant height, root length, shoot dry weight, root dry weight, root/shoot ratio and total dry weight) together with WTC (waterlogging tolerance coefficient) of all the traits were investigated and analyzed. The waterlogging tolerance of the 118 DH lines and their parents were evaluated. 【Result】 The results showed that: the six responsive traits changed significantly under the waterlogging conditions compared with the control in the experiment and the effects of waterlogging were much more severe on RDW than other traits. All of the six traits and WTC of all the traits showed transgressive and continuous distribution under both waterlogging and control conditions. The correlation among all the traits investigated in the study indicated that WTC of SDW, RDW and TDW could be used as available evaluation indices for waterlogging tolerance. 【Conclusion】 Lines 005, 007, and 040 screened from the population are the most waterlogging-tolerant lines and 086, 110, and 119 are the most waterlogging-sensitive lines.

    PLANT PROTECTION

    cDNA-AFLP Analysis of Near-isogenic Line TcLr38 Resistance to Wheat Leaf Rust

    WANG Wen-xia,CHU Dong,GAO Shi-gang,YAN Hong-fei,GOU Li-qian,YANG Wen-xiang,LIU Da-qun
    Scientia Agricultura Sinica. 2010, 43(2):  293-303 .  doi:10.3864/j.issn.0578-1752.2010.02.009
    Abstract ( 1139 )   PDF (669KB) ( 982 )   Save
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    【Objective】 The objective of the research is to analyze the gene expression profile of wheat near-isogenic line TcLr38 induced by Puccinia triticina and to detect the fragments related to disease-resistant gene expression. 【Method】 The study was carried out to detect the differential expression of genes in TcLr38 and Thatcher inoculated with avirlent P. triticina strain 05-22-65 (THTS) by complementary DNA amplified fragments length polymorphism (cDNA-AFLP) analysis. 【Result】 Seventy-six pairs of primers were used to amplify products for cDNA-AFLP analysis. About 3800 bands were detected and the average number of bands per pair of primers amplified was 50. Twenty-eight of 76 primer combinations for cDNA-AFLP analysis amplified specific bands. Polymorphic bands between TcLr38 and Thatcher were grouped into eight different types, and three types of them were supposed to correspond to the disease-resistant reactions. Ninety-five transcripts derived fragments (TDFs) were selected for their differential expression from wheat near-isogenic line TcLr38 and Thatcher. And nineteen TDFs were upregulated and only expressed in the inoculated TcLr38, whereas 8 TDFs were suppressed. Twenty-one TDFs were sequenced and analyzed. BLASTx analysis showed that 17 TDFs had the homologous sequences with the sequence in GenBank, and fifteen were homologous with the known function genes. 【Conclusion】 The TDFs confer protein kinase C, ATP binding protein, receptor-like kinase, signal transduction histidine kinase, putative dnaK suppressor protein DksA, Methionine-tRNA synthetase, Peptidase M23, Ran GTPase activating protein 1, and Enoyl-Coenzyme A hydratase were supposed to correspond to the expression of the disease-resistant reactions.

    Molecular Detection of Phytoplasma Strains From Several Plants Around Diseased Paulownia Infected with Paulownia Witches’-broom Phytoplasma
    WANG Jie,TIAN Guo-zhong,XU Qi-cong,LIU Yong-guang,GAO Rui,LI Xiang-dong,ZHU Xiao-ping
    Scientia Agricultura Sinica. 2010, 43(2):  304-312 .  doi:10.3864/j.issn.0578-1752.2010.02.010
    Abstract ( 1057 )   PDF (542KB) ( 766 )   Save
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    【Objective】 The objective of this study is to investigate and identify the alternative host plants of paulownia witches’-broom phytoplasma (PaWB) in natural condition. 【Method】 Using universal primers for phytoplasmal 16S rRNA gene, nested PCR was conducted to detect phytoplasmas from 16 species of plants with/without symptoms of yellowing, little leaf, winkle leaf, witches’-broom and growing around paulownia plants infected with PaWB, and then sequencing and phylogenetic analyses of the amplified fragment were made. Indirect immunofluorescence was also used to analyze partial plant samples.【Result】A specific fragment of ca. 1.2 kb in length was amplified with nested PCR from 7 species of plants, Chinese yam (Dioscorea opposita), groundcherry (Physalis angulata), pepper (Capsicum annuum), peanut (Arachis hypogaea), goosegrass (Eleusine indica), bristle grass (Setaria viridis) and musdky guard (Cucurbita moschata) along with the positive control of PaWB. The sequencing and phylogenetic results showed that all the phytoplasmas detected in the 7 species of plants and the one from diseased paulownias belonged to 16Sr I-D subgroup of aster yellows phytoplasma group. Indirect immunofluorescence analysis using antibody to PaWB Tuf protein indicated that specific fluorescence was easily detected in paulownia plants infected with Beijing and Heze isolates of PaWB, but not either in healthy paulownia plants or in CaWL, DoY, AhY, CmA phytoplasma-infected plants. 【Conclusion】 Paulownia witches’-broom phytoplasma were detected from 7 species of plants, which were probable natural hosts of PaWB.

    Genome-wide Microarray Analysis of Helicoverpa armigera Larva Fed on Transgenic hpa1Xoo Cotton Leaves
    MIAO Wei-guo,WU Shu-wen,SONG Cong-feng,WANG Yu,GONG Xiao-chong,ZHANG Liang,WANG Jin-sheng
    Scientia Agricultura Sinica. 2010, 43(2):  313-321 .  doi:10.3864/j.issn.0578-1752.2010.02.011
    Abstract ( 994 )   PDF (444KB) ( 676 )   Save
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    【Objective】 The objective of this study was to confirm the resistance of transgenic hpa1Xoo cotton T-34 to cotton bollworm (Helicoverpa armigera) and its resistance mechanism. 【Method】 Bioassay and 23K Oligo silkworm microarray were used to analyze the insect resistance of T-34 and globe transcriptome profile of H. armigera kept by transgenic hpa1Xoo cotton leaves comparing to untransformed cotton Z35. 【Result】 The fresh leaves of T-34 and Z35 were utilized to keep H. armigera larva, and only T-34 made H. armigera larval development become slow rather than Z35. Microarray data indicated that 872 genes in H. armigera were totally deregulated by the comparison of T-34 vs Z35. Of 872 genes, 328 genes were showed to be upregulated (ratio>2.0), and 544 genes were indicated to be downregulated (ratio<0.5). It was suggested that all differentially expressed genes might be classified and related to 13 biological functions, and involved in 96 biological pathways. 【Conclusion】 harpinXoo encoded by hpa1Xoo confers the resistance of transgenic hpa1Xoo cotton to H. armigera, and influences the multiple metabolism pathways in H. armigera larva.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Effects of Long-Term Application of Sulphur-Containing and Chloride-Containing Chemical Fertilizers on Yield and Its Components of Rice
    SHEN Pu,LI Dong-chu,GAO Ju-sheng,XU Ming-gang,WANG Bo-ren,HOU Xiao-juan
    Scientia Agricultura Sinica. 2010, 43(2):  322-328 .  doi:10.3864/j.issn.0578-1752.2010.02.012
    Abstract ( 1142 )   PDF (336KB) ( 857 )   Save
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    【Objective】 Effects of long-term application of sulphur-containing and chloride-containing chemical fertilizers on yield and its components of rice were studied in southern red soil. 【Method】 The concentration of SO42- and Cl- in paddy soil, and its relationship with yield of early and late rice were analyzed after long-term application sulphur-containing and chloride-containing, chemical fertilizers using the statistical method. 【Result】 The concentration of SO42--S in paddy soil increased first and then decreased later after long-term application of sulphur-containing chemical fertilizers, and total yield of early and late rice were showed a negative correlation with concentration of SO42--S significantly. The average yields of early rice (1982-2008) were 5 738.9, 5 657.5, 5 633.8 kg?hm-2, and the yields of late rice were 4 831.9, 4 603.8, and 4 557.0 kg?hm-2, respectively, after long-term application of Cl-, Cl-+SO42-, SO42-fertilizers. The yield was the highest under Cl- treatment. Yield of rice in SO42- treatment declined rapidly after 15 years of fertilization and was less than that of Cl- treatment significantly, but increased slowly after 2000, and the yield of the three treatments showed no significant difference. The concentration of SO42--S maintained 26.50 mg?kg-1, and the symptom of sulphur lack did not appear. 【Conclusion】 The concentration of SO42- was accumulated easily in southern hilly area paddy soil after long-term application of sulphur-containing fertilizers, which resulted in the risks of rice growth, the effects of long-term application of chloride-containing fertilizers was better than sulphur-containing fertilizers.

    The Possible Effects of Global Warming on Cropping Systems in China Ⅰ. The Possible Effects of Climate Warming on Northern Limits of Cropping Systems and Crop Yields in China
    YANG Xiao-guang,LIU Zhi-juan,CHEN Fu
    Scientia Agricultura Sinica. 2010, 43(2):  329-336 .  doi:10.3864/j.issn.0578-1752.2010.02.013
    Abstract ( 1638 )   PDF (476KB) ( 3214 )   Save
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    【Objective】 Increasing temperature in China significantly since the 1980s has become a consensus in the context of global climate change. How the change affected the agriculture or even the cropping system has attracted more and more attention of the government and scientists. In this study, the possible effects of climate warming on the countrywide northern limits of cropping systems, the northern limits of winter wheat and double rice, and the stable-yield northern limits of rainfed winter wheat - summer maize rotation in China from 1981 to 2007 were analyzed. And the possible changes of crop yield due to the changes of planting limits during the period from 1950s to 1981 and the period from 1981 to 2007 were compared and discussed. 【Method】 A recognized calculation method of agro-meteorological indicators were used in the study. The countrywide northern limits of cropping systems, the northern limits of winter wheat and double rice and the stable-yield northern limits of rainfed winter wheat–summer maize rotation were draw by ArcGis for two periods from 1950s to 1980 and 1981 to 2007, according to the indices of the climate zoning index of cropping system, winter wheat and double rice northern limits and stable-yield northern limits of rainfed winter wheat-summer maize rotaion. 【Result】 Compared with the results during the period from 1950s to 1980, the northern limits of the two-cropping system from 1981 to 2007 occurred significant spatial displacement in Shaanxi, Shanxi, Hebei, Beijing and Liaoning provinces and city. The northern limits of the three-cropping system occurred largest spatial displacement in Hunan, Hubei, Anhui, Jiangsu, and Zhejiang provinces. Without the consideration for variety and social economic changing, grain yield per hectare of main cropping pattern could increase about 54%-106% if the one-cropping system changed to two-cropping system, while increase about 27%-58% if two-cropping system is changed to three-cropping. The northern limits of winter wheat moved northwards and westwards to different degrees in Liaoning, Hebei, Shanxi, Shaanxi, Inner Mongolia, Ningxia, Gansu and Qinghai provinces,comparing with the results during the 1950s-1980 period. For example in Hebei province, northern limits of winter wheat moved north,leading to about 25% grain yield increase in the changing area if the cropping system is changed from spring wheat to winter wheat in one year. The northern limits of double rice cropping area in Zhejiang, Anhui, Hubei, and Hunan provinces moved northwards. Considering the heat resource, the change of limits can increase the grain yield. The stable-yield northern limits of rainfed winter wheat-summer maize moved southeastwards in most regions, mainly caused by the decreased rainfall during recent years in the study areas.【Conclusion】 During the past 50 years, the climate warming caused the northwards movement of the northern limits of cropping system, and the northern limits of winter wheat and double rice. The changes may increase the unit grain yield in the changing area. However, the stable-yield northern limits of rainfed winter wheat–summer maize rotation moved southeastwards due to the decreasing rainfall.
    HORTICULTURE
    Effects of Water Supply Tension on Yield and Water Use Efficiency of Greenhouse Cucumber
    LI Shao,XUE Xu-zhang,GUO Wen-shan,LI Xia,CHEN Fei
    Scientia Agricultura Sinica. 2010, 43(2):  337-345 .  doi:10.3864/j.issn.0578-1752.2010.02.014
    Abstract ( 1098 )   PDF (398KB) ( 757 )   Save
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    【Objective】 The effects of different water supply tension(WST) that is different soil moisture on the growth and development of greenhouse cucumber (Cucumis sativus L.) were studied. The demand of water at different growth stages of greenhouse cucumber was determined to provide a theoretical basis for the irrigation decision-making. 【Method】 A negative pressure water supplying and controlling pot device was used to control different soil water contents by setting the WST of the device at different values. The differences in photosynthetic rate, yield and water use efficiency(WUE) of cucumber under different WST were analyzed. 【Result】 The results showed that the negative pressure water supplying and controlling pot device can maintain soil water content in a enactment state by setting the WST. The leaf photosynthetic rate, biomass accumulation as well as yield and marketable fruit rate of cucumber were much higher at 3-5 kPa WST treatments, the highest WUE 36.57 appeared in WST of 7 kPa and a significant lower WUE was observed in WST of 1 kPa, differences in WUE for other 6 WST were not significant. The result shows that WUE can only by improved by control water supply at different growth stages of cucumber. The result also indicates that the water demand increase with the growing process. The growth will be restricted and yield will be reduced with the shortage of water supply, increase the yield at terminal fruiting stage can be obtained by increasing soil water content at fruiting stage of cucumber. 【Conclusion】 The above results indicate that the range of WST from 3-5 kPa are more suitable for greenhouse cucumber growth when the relative soil water content ranges from 67% to 81%. Reduce water supply at seedling stage, control water supply at flowering stage and increase water supply at fruiting stage of cucumber can increase yield and WUE.

    Genetic Analysis and Gene Localization of Leaf Color(purple/green) in Heading Cabbage
    ZHANG Cheng-he,LIU Chuan-bin,REN Yan-rui,XUAN Shu-xin,SHEN Shu-xing
    Scientia Agricultura Sinica. 2010, 43(2):  346-350 .  doi:10.3864/j.issn.0578-1752.2010.02.015
    Abstract ( 1041 )   PDF (236KB) ( 698 )   Save
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    【Objective】 The aim of the experiment is to clarify the inheritance pattern and the gene localization of leaf color in heading cabbage(Brassica oleracea L.) 【Method】 The common heading cabbage 9601 with green color leaf and its trisomics were crossed with purple cabbage Ziyang respectively by artificial castration and pollination. Next year, the trisomic plants (2n+1) were selected from each F1 population by morphological observation and cytological identification and were crossed with the common cabbage 9601. The inheritance pattern of leaf color and gene localization were conducted by disomic analysis and trisomic analysis and χ2 test. 【Result】 Disomic analysis showed that leaf color (purple/green)of the cabbage was controlled by two independent genes with addition effect, and the purple color was dominant to green color. Trisomic analysis indicated that two genes controlling the leaf color were on the chromosome 3 and chromosome 8, respectively. 【Conclusion】 The leaf color of the cabbage was determined by two independent genes, and located on the chromosome 3 and chromosome 8, respectively.

    Relationship Between Expressions of GalDH and GalLDH and Ascorbate Content in Apple Fruits
    LI Ming-jun,GAO Jing,MA Feng-wang,LIANG Dong,HOU Chang-ming
    Scientia Agricultura Sinica. 2010, 43(2):  351-357 .  doi:10.3864/j.issn.0578-1752.2010.02.016
    Abstract ( 908 )   PDF (366KB) ( 614 )   Save
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    【Objective】 The objective of this experiment was to further investigate whether apple fruit has the capability of AsA biosynthesis. 【Method】 L-galactose dehydrogenase (GalDH) cDNA which encode a enzyme involved in synthesizing AsA in plants was isolated from young fruit of Malus domestica Borkch cv. Gala,then the relationship of the mRNA expression level and enzyme activities of GalDH and L-galactono-1,4-lactone dehydrogenase(GalLDH) on AsA in different tissues of apple was studied. 【Result】 cDNA of GalDH, which was cloned from young fruit exocarp of apple, has an 975 bp open reading frame and encodes a protein of 324 amino acid residues with a molecular mass of 34.97 kD, its accession no in GenBank is GQ131419. The mRNA expression levels and enzyme activities of GalDH and GalLDH were detected in leaves as well as different tissues of apple fruits. Both of mRNA expression levels and activities of the two enzymes in leaves were higher than that in fruit, in young fruit was higher than that in mature fruit, while in peel was higher than that in flesh. Meanwhile, AsA content in apple peel was regulated by light condition. The mRNA expression levels and enzyme activities of both in sun side peel were higher than that in shaded side peel, but no significant differences between sun side flesh and shaded side flesh. A significant positive correlation was detected between AsA content and enzymes activities of GalDH and GalLDH in different tissues of apple. 【Conclusion】 These results further suggest that apple fruit has the capability of AsA biosynthesis by L-galactose pathway,and synthesis may be a major reason of AsA formation in apple fruit.

    Study on Method of Constructing Core Collection of Malus sieversii Based on Quantitative Traits
    LIU Zun-chun,ZHANG Chun-yu,ZHANG Yan-min,ZHANG Xiao-yan,WU Chuan-jin,WANG Hai-bo,SHI Jun,CHEN Xue-sen
    Scientia Agricultura Sinica. 2010, 43(2):  358-370 .  doi:10.3864/j.issn.0578-1752.2010.02.017
    Abstract ( 1088 )   PDF (393KB) ( 1104 )   Save
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    【Objective】 The genetic diversity of 15 quantitative traits from 300 Malus sieversii accessions was used to study the method of constructing Malus sieversii core collection. 【Method】 A total of 24 Malus sieversii core collections were constructed at 30% sampling proportion by two genetic distances, including Euclidean distance and Mahalanobis distance, combined with four cluster methods including UPGMA, Ward’s method, Complete linkage and Single linkage, and with three sampling methods including random sampling, deviation sampling and preferred sampling, respectively, using stepwise clustering. Using the best constructing method, the optimum sampling proportion was filtrated under seven different sampling proportions (10%, 15%, 20%, 25%, 30%, 35%, and 40%). 【Result】 In constructing Malus sieversii core collection, Euclidean distance was much better than Mahalanobis distance. Comparing the four cluster methods, the single linkage was better than UPGMA, Ward’s method and Complete linkage for constructing core collection. The preferred sampling was more suitable than deviation sampling, although the deviation sampling could also significantly increase VD%, CR% and VR%. Twenty percent was the optimum sampling proportion.【Conclusion】 At 20% sampling proportion, using stepwise clustering, Mahalanobis distance and Single linkage method combined with preferred sampling can construct the most reprehensive core collection and is the most suitable method for constructing Malus sieversii core collection.

    Establishment and Optimization of Selectively Amplified Microsatellite (SAM) Analysis System on Chinese Jujube (Zizyphus jujuba Mill.)
    MA Qing-hua,WANG Gui-xi,LIANG Li-song
    Scientia Agricultura Sinica. 2010, 43(2):  371-379 .  doi:10.3864/j.issn.0578-1752.2010.02.018
    Abstract ( 1054 )   PDF (673KB) ( 534 )   Save
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    【Objective】 The objective of this study is to establish and optimize the SAM (selectively amplified microsatellite) analysis system, enrich the research methods in population genetics of Chinese jujube and provide reference and templets for SSR primer development using SAM in Chinese jujube. 【Method】 High-quality genomic DNA was isolated by improved CTAB method from leaves of different jujube cultivars (Dongzao, Dalilongzao and Jinsixiaozao). The SAM reaction system was established by optimizing enzyme digestion and ligation reaction, suppression-amplification, pre-amplification and SAM selective amplification. 【Result】 Two-step-enzyme digestion and ligation reaction was approved to be the key step in the whole system. In the suppression- amplification (the total volume was 20.0 μL), the suitable template volume was tested to be containing 2.0 μL of the production of digestion and ligation reaction, in pre-amplification and SAM selective-amplification (the total volume was 20.0 μL), the suitable template volume was 2.0 μL of the diluted production of the above amplification reaction. In the suppression-amplification and pre-amplification, 0.5 U ExTaq was used in the reaction mixture, and then the two amplifications were performed with 25 cycles, respectively. After reaction, the products were diluted 1:20 with TE buffer. In the SAM selective-amplification, the annealing temperature was performed by reducing 1℃ per cycle in the first 6 cycles, and by adding ExTaq to 1.0 U for the accuracy of the amplification. 【Conclusion】 16 pairs of MseⅠadapter +NN primer and SSR primer (PCT6) were designed for the SAM selective-amplification, all of the 16 primer-combinations revealed sufficient amplified bands with high polymorphism. The established SAM reaction system could be used to study the population structure, genetic diversity and the SSR primer development in the population of Chinese jujube.

    Screening for Heat-Tolerant Variants and Field Identification of Gerbera hybrida

    PENG Jian-zong,LI An,HUANG Zhi-gang,CHEN Zhao-ping,WEN Fang-de,WANG Xiao-jing
    Scientia Agricultura Sinica. 2010, 43(2):  380-387 .  doi:10.3864/j.issn.0578-1752.2010.02.019
    Abstract ( 859 )   PDF (352KB) ( 755 )   Save
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    【Objective】 The objective of the experiment is to screen heat-tolerant variants in Gerbera hybrida with Shenzhen 5 (S5) as material. 【Method】 Heat tolerant lines EH and H10 were obtained by in vitro screening with treatments of EMS and intermittent high temperature. After rooting, the regenerated plants were transferred to field. The growth and some physiological and biochemical changes of leaf, the yield and quality of cut flower under natural high temperature environment were determined. 【Result】 Compared with the control, the leaf area of H10 was larger and chlorophyll content was higher. In July and August, the hottest months of the year in Zhuhai, the flower yield of H10 increased by 55.67% and 53.27% and flower diameter increased by 5.57% and 5.70%, scape length increased by 10.35% and 11.77%, respectively, and the anthocyanin content of H10 petals was also obviously higher than that of the control. Moreover, H10 showed higher membrane stability with lower permeability, higher proline content and increased activities of superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX). Nevertheless, EH had no significant improvement in the characters of plant growth, flower yield and quality under natural high temperature conditions. 【Conclusion】 H10 would be an excellent heat-tolerant variant of G. hybrida from the field identification and determination of physiological and biochemical changes.

    STORAGE·FRESH-KEEPING·PROCESSING
    Effect of Added Gluten on Pasting Properties of Wheat Starch
    CHEN Jian-sheng,DENG Zhi-ying,WU Peng,TIAN Ji-chun,XIE Quan-gang
    Scientia Agricultura Sinica. 2010, 43(2):  388-395 .  doi:10.3864/j.issn.0578-1752.2010.02.020
    Abstract ( 987 )   PDF (408KB) ( 1180 )   Save
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    【Objective】 Effect of gluten on pasting properties of wheat starch was studied to provide a scientific basis for application of gluten in food production and quality improvement in wheat breeding. 【Method】 The pasting properties of blends were analyzed using PH1391 wheat starch mixed with five different additions of three kinds of gluten (strong-gluten, medium-gluten and weak-gluten) and the structures of network were also observed using microscope. 【Result】 Obvious downtrends of peak viscosity, trough viscosity, final viscosity, area of viscosity, setback and peak time were found with increasing addition of gluten. In general, in gluten addition increased by 2% decreased the average value of them respectively by 3.6%,4.8%,3.4%,3.8%,4.0% and 1.18% of those corresponding indexes of pure starch. Descent rate of the indexes mentioned above exceeded more than 2% except peak time,but there were no significant influence of gluten addition on breakdown, pasting temperature and pasting time. The interlayer composed of gluten could not be observed when the addition of gluten was 10%, as the compound formed of gluten inlayed in the paste of starch,but obvious greater interlayer was detected when the addition of gluten was 18%. There was significantly or remarked difference among the effects of three different kinds of gluten on the peak viscosity, trough viscosity, area of viscosity, setback and peak time, but it had no distinctly difference among the effects of different glutens on pasting temperature and pasting time. Descending order of the effect of different glutens on peak viscosity, trough and area of viscosity was strong-gluten, medium-gluten, and weak-gluten, but the sequence of them on setback was opposite. 【Conclusion】 Both addition and type of gluten affected significantly on peak viscosity,trough, area of viscosity, setback and peak time, but there was no significant different effects on peak time and peak temperature.

    ANIMAL SCIENCE·RESOURCE INSECT
    Cloning and Expression of cDNA of Bovine Neutrophil β-defensin12 from Holstein Cow and Its Antibacterial Activity
    WU Jian-ming,WANG Chang-fa,HE Hong-bin,HU Gui-xue,YANG Hong-jun,YANG Shao-hua,GAO Yun-dong,ZHONG Ji-feng
    Scientia Agricultura Sinica. 2010, 43(2):  396-403 .  doi:10.3864/j.issn.0578-1752.2010.02.021
    Abstract ( 973 )   PDF (718KB) ( 693 )   Save
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    【Objective】 The gene coding the bovine neutrophil beta-defensin12 was cloned from Holstein cow and the antibacterial activity of the recombinant protein was analyzed. 【Method】 BNBD12 gene was amplified using reverse transcription-polymerase chain reaction (RT-PCR). The PCR product was analyzed, and the mature peptide gene was synthesized and inserted into vector pET32a(+) to construct plasmid pET32a(+)/BNBD12, then the plasmid was expressed in E. coli BL21 (DE3) cell that induced by IPTG. The antibacterial activity was assayed by agar hole diffusion-inhibition zone method and electron microscope using the purified recombination protein against E. coli and Sta. aureu. 【Result】 Sequence analysis showed the amplicon 180 bp encoding a polypeptide of 60 amino acids. SDS-PAGE and Western blotting results showed that the recombinant proteins were expressed in E. coli with molecular weight of 26 kD and the recombinant proteins accounted for 21.4% of the whole proteins. The purified protein BNBD12 0.05 mg?mL-1 had antibacterial activity and killed pathogenic bacteria by leaking the content out in vitro against E. coli and Sta. aureus. 【Conclusion】 The results showed that BNBD12 protein is well expressed in E. coli and it has antibacterial activity against E. coli and Sta. aureus.

    Effect of DNA Vaccine pcISI Harboring Two Copies of Inhibin on Follicle and Corpus Luteum of Beef Cattle
    WANG Shui-lian,XUE Li-qun,DENG Li-xin,CUI Xian-li,YANG Li-guo
    Scientia Agricultura Sinica. 2010, 43(2):  404-410 .  doi:10.3864/j.issn.0578-1752.2010.02.022
    Abstract ( 1180 )   PDF (283KB) ( 586 )   Save
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    【Objective】 The effect of four different dosages of inhibin gene expressing plasmid on the reproductive capacity of the beef cattle was studied. 【Method】 Fifty eight synchronized beef cattle were randomly assigned to four dosage groups (T1, T2, T3, or T4 , immunized with 0.75, 1.5, 2.25, or 3.0mg of DNA vaccine pCISI harboring two copies of INHal-32, respectively) and two control groups (C1 ,which was injected with 3.0mg pcMV-S plasmid per cattle and C2 , which was injected with physiological saline 3 mL per cattle) to investigate the effect of inhibin gene immunization on development of follicle and corpus luteum, and the booster immunization was given to the cattle on day 21 after the primary immunization. 【Result】 Animals in the four dosage groups had a greater number of large follicles (≥10 mm) than those in group C, and the number of large follicles was significantly larger than those in group C (P<0.05). Compared to the control group, the number of medium and small follicles in T3 was much larger (P<0.05), the same as T4. Moreover, luteum corpuses on bilateral ovaries of beef cattle in the four-dosage inhibin gene vaccine-immunized groups were significantly larger than those in group C (P<0.05). Among the four dosage groups, the largest luteum corpus was in T3, the smallest was in T1, there was a significant difference between T3 and T1. Besides, on day 10 after booster immunization, there was a difference in correlation coefficient between the content of inhibin antibody and size of the mature follicles (r = 0.629, P<0.05), and the content of inhibin antibody and the size of the corpus lutuem was significantly different (r = 0.651, P<0.01). 【Conclusion】 These findings demonstrate that inhibin gene vaccine pcISI is able to stimulate development of follicle and corpus luteum. Dosage of 2.25 mg is the best dosage in the study.

    VETERINARY SCIENCE
    Sequencing and Analysis of Complete Genome of a Porcine Circovirus Type 2-like Agent P1
    WEN Li-bin,HE Kong-wang,YANG Han-chun
    Scientia Agricultura Sinica. 2010, 43(2):  411-416 .  doi:10.3864/j.issn.0578-1752.2010.02.023
    Abstract ( 1149 )   PDF (277KB) ( 597 )   Save
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    【Objective】 The aim of the experiment is to discover an agent containing high homologous sequences of porcine circovirus type 2 in swine. 【Method】 PCR was developed for detection of DNA extracted from porcine sera collected from pigs with clinical signs of postweaning multisystemic wasting syndrome, then a reverse PCR was carried out based on the acquired sequences in this study, the sequences of PCR fragments were assembled into consecutive sequence and then finally verified by amplifing the agent complete genome. 【Result】 Complete nucleotide sequence of genome of porcine circovirus type 2-like agent P1 was firstly reported in this paper. P1 contained circular genomic DNAs of 648 nucleotides and three open reading frames. Except 16 nucleotides in the 5′ end of the genome, the genome of P1 showed 98.42% of nucleotide homology compared with porcine circovirus type 2 BF isolate. Phylogenetic analysis revealed that P1 was closely related to the porcine circovirus. 【Conclusion】 Results suggested that there existed porcine circovirus type 2-like agent P1 in swine.

    Influence of Combined Exposure to Lead and Cadmium on Bcl-2, Bax and c-fos mRNA Expression in Neonatal Brain and the Protective Effects of N-Acetylcysteine on Pregnant Rats
    LU Hao,DA Jian-sen,MEI Li,ZHANG Ying,LIU Zong-ping
    Scientia Agricultura Sinica. 2010, 43(2):  417-423 .  doi:10.3864/j.issn.0578-1752.2010.02.024
    Abstract ( 869 )   PDF (391KB) ( 516 )   Save
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    【Objective】 In order to observe the impact of low-level lead or/and cadmium and protective effect of N-Acetylcysteine in cortical neurons of neonatal SD rats, the cultured cortical neurons of neonatal rats were used to perform the toxicity test. 【Method】 Thirty-five adult female SD rats were divided into control group, lead acetate group (300 mg?L-1), lead acetate + NAC group (300 mg?L-1 + 20 mmol?L-1), cadmium chloride group (10 mg?L-1), cadmium chloride + NAC group (10 mg?L-1+20 mmol?L-1), group of lead and cadmium (300 mg?L-1 +10 mg?L-1), group of lead and cadmium + NAC (300 mg?L-1 +10 mg?L-1+ 20 mmol?L-1), and the additives were given in the drinking water. After gestational periods the expressions of bcl-2, Bax and c-fos mRNA in brain of neonatal rats were detected by real time fluorescent quantitative PCR. 【Result】 In comparison with the control group, there was a significant decrease in the expression of bcl-2 mRNA except for B group (P<0.05), and the expression of Bax and c-fos mRNA appeared a significant increase (P<0.05), especially F group. Compared NAC protective groups with the poisoning groups, the expression of bcl-2 mRNA increased to a certain extent (P<0.05 or P>0.05), but the expression of Bax and c-fos mRNA significantly decreased (P<0.05). 【Conclusion】 The results showed that lead and cadmium appeared a synergetic effect, and it indicated that NAC could protect apoptosis of brain cells caused by exceptional gene expression from lead and/or cadmium in neonatal SD rats.

    Effects of Duck Infected by Muscovy Duck Reovirus on Humoral Immunity Function

    WANG Quan-xi,WU Bao-cheng,LI Guo-ping,HUANG Yi-fan
    Scientia Agricultura Sinica. 2010, 43(2):  424-429 .  doi:10.3864/j.issn.0578-1752.2010.02.025
    Abstract ( 1222 )   PDF (277KB) ( 714 )   Save
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    【Objective】 The purpose of the test was to investigate the effect of muscovy duck infected by muscovy duck reovirus on humoral immunity. 【Method】 Sixty healthy ducks at 5-day-old of age were randomly divided into two groups . The experimental group was injected 0.2ml muscovy duck reovirus (one penent of TCID50, TCID50=10-3.7558) on leg, and the controll group was injected normal saline at the same quantity. The quantity of plasma cells in bursa fabricius and the spleen was tested by the histochemical test method. The level of avian influenza antibodies in serum was tested by the method of indirect hemagglutination, also the content change of IL-2 and IL-6 in the serum was tested by the testing method of radiation immunization. 【Result】 Results showed that the quantity of plasma cells in bursa of fabricius and spleen in the experiment group was less than the controll group, had different degrees of decrease respectively during the time after attack test by using reovirus, especially after 15 day of infection the antibody of experiment group was the lowest (P<0.01). The ability of antibody-producing decreased too. The avian influence antibody of experiment group was very significantly less than the controll group (P<0.01), especially after 15 day of infection the antibody of experiment group was the lowest. But they all increased at 15 days after attack test .The content of IL-2 and IL-6 in the serum of experiment group ducks decreased on the first stage of the test ,but they all increased on the later stage compared with the former. However the content of IL-2 and IL-6 in the serum of experiment group ducks was lower than the control group (P<0.01). 【Conclusion】 Ducks at five day old of age are infected by muscovy duck reovirus, plasma cells in immune organs (bursa of fabricius and spleen) are damaged and the ability of antibody forming decrease. The immunity function of ducks decrease because the virus decreased the content of immunity molecular (IL-2, IL-6) in serum. Therefore it is an effective way to enhance the humoral immunity of duck to control muscovy duck reovirus.

    RESEARCH NOTES
    Genes Related to Fuzz Initiation and Development in Gossypium arboretum Identified by cDNA Microarray
    ZHAO Guo-hong,WANG Sheng,JIA Yin-hua,SUN Jun-ling,WANG Jie,DU Xiong-ming
    Scientia Agricultura Sinica. 2010, 43(2):  430-437 .  doi:10.3864/j.issn.0578-1752.2010.02.026
    Abstract ( 1092 )   PDF (415KB) ( 628 )   Save
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    【Objective】 The objective of this study is to isolate genes related to fuzz initiation and development in Gossypium arboretum. 【Method】 The filter arrays of cDNAs were performed to identify the differentiation expressed genes between the wild-type DPL971 and its fuzz mutant line DPL972 in the early developing stages of -3DPA, 0DPA, +3DPA, +5DPA, and +7DPA. Compared with the database of model plant Arabodopsis thaliana, some genes related to the initiation of the cotton fiber were isolated, and part of them have been verified by RT-PCR and QRT-PCR. 【Result】 Eight genes related to fiber initiation and development in Gossypium arboretum were identified, and seven of them, KAK(DR461366), MYB5(ES812048), TTG1(ES811600), MYB23(DR453866), CSLD3(DR459646), RHD2(DR461821), ZWI(ES791383), were highly homologous to the Arabodopsis proteins which played important roles in the initiation of the trichomes development in Arabidopsis. The result of RT-PCR and QRT-PCR indicated that the 5 genes of MYB23, MYB5, TTG1, CSLD3, and RHD2 had significantly different expressions in +3DPA ovules between the fuzz mutant line DPL972 and its wild line DPL971. 【Conclusion】 It implied that these genes may be related to the initiation of the fuzz fiber, the first gene may inhibit the fuzz differentiation and the latter four may promote the fuzz development.

    Analysis of SRAP Markers Associated with Common Leaf Spot Disease Resistance Gene in Alfalfa
    WANG Yu,YUAN Qing-hua,LI Xiang-lin,GAO Jian-ming
    Scientia Agricultura Sinica. 2010, 43(2):  438-442 .  doi:10.3864/j.issn.0578-1752.2010.02.027
    Abstract ( 899 )   PDF (327KB) ( 936 )   Save
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    【Objective】 The study identified the molecular markers linked to common leaf spot disease resistance gene in alfalfa. 【Method】 With a F1 population produced by two middling resistant parents (YL0602M×SH0602M), the molecular marker associated with common leaf spot disease resistance gene was screened by BSA method and SRAP technology. 【Result】 One specific band was found between resistant and susceptible DNA pool and 20 plants that comprised the two pools. Sequencing of the fragment indicated that its length was 169 bp. This SRAP marker was named as M3E3-R169. 【Conclusion】 The coincidence rate of the existence of M3E3-R169 in 20 plants was 80%, so M3E3-R169 would be linked to common leaf spot disease resistance gene.