表皮蛋白基因TcCP14.6和TcLCPA3A参与介导赤拟谷盗对磷化氢的抗性形成

doi:10.3864/j.issn.0578-1752.2022.11.006

Abstract

Abstract:

【Objective】 Cuticle proteins (CPs) are the main components of insect cuticle, and numerous studies have confirmed that the CP genes were involved in insecticide resistance. The objective of this study is to clarify the roles of TcCP14.6 (cuticle protein CP14.6) and TcLCPA3A (larval cuticle protein A3A) in phosphine resistance of Tribolium castaneum.【Method】 The FAO (Food and Agriculture Organization of the United Nations)-recommended bioassay method was used to determine the phosphine resistance levels of five different T. castaneum populations. The TcCP14.6 and TcLCPA3A sequences were downloaded from the T. castaneum genome data, and their encoded amino acid sequences, signal peptides and conserved domains were predicted via the online services. The total RNAs were extracted from different tissues (the cuticles of head, thorax and abdomen, wing, leg, gut, Malpighian tubules and fat body), different phosphine resistance levels, as well as phosphine induction of T. castaneum, respectively. With TcRPS and TcRPL as internal reference genes, the RT-qPCR was used to analyze the expression patterns of TcCP14.6 and TcLCPA3A in different tissues, different phosphine resistance levels, and in response to phosphine induction. Lastly, the RNA interference (RNAi) technology and bioassay method were used to explore the relationship between the two CP genes and phosphine resistance in T. castaneum.【Result】 The bioassay analysis showed that Jiangsu (JS, RR=1.7) and Yunnan (YN, RR=3.0) belonged to susceptible populations, Hunan (HN, RR=20.2) belonged to moderately resistant population, Sichuan (SC, RR=395.4) and Guangdong (GD, RR=862.7) belonged to highly resistant populations. The sequence analysis demonstrated that both TcCP14.6 and TcLCPA3A proteins consisted of signal peptides and chitin binding domains. The RT-qPCR analysis suggested that TcCP14.6 and TcLCPA3A all had a higher expression in the peripheral tissues (the cuticles of head, thorax and abdomen, wings and legs) of T. castaneum, and with a lower expression in the internal tissues, such as fat body, gut and Malpighian tube. Besides, with the increase of phosphine resistance levels in T. castaneum, the expression level of TcCP14.6 was up-regulated, while the expression level of TcLCPA3A was down-regulated. After phosphine induction for 6 h in T. castaneum, the expression levels of TcCP14.6 and TcLCPA3A were up-regulated and down-regulated, respectively. The injection of dsRNA could significantly inhibit the expression of TcCP14.6 and TcLCPA3A in phosphine resistance (GD) and susceptible (YN) populations of T. castaneum. When treated with phosphine (LC₃₀), the mortality significantly increased after the TcCP14.6 was silenced. Instead, the mortality significantly decreased after the TcLCPA3A was silenced.【Conclusion】 The two CP genes TcCP14.6 and TcLCPA3A are involved in phosphine resistance of T. castaneum.

Key words: Tribolium castaneum, phosphine resistance, cuticle protein, TcCP14.6, TcLCPA3A, RNA interference (RNAi)

CHEN ErHu,MENG HongJie,CHEN Yan,TANG PeiAn. Cuticle Protein Genes TcCP14.6 and TcLCPA3A are Involved in Phosphine Resistance of Tribolium castaneum[J].Scientia Agricultura Sinica, 2022, 55(11): 2150-2160.

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References 46

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引物类型Primer type	引物名称Primer name	引物序列Primer sequence (5′ to 3′)
qPCR引物 Primers for qPCR	TcCP14.6-F	CGGCCATCCTCAGACTCAAC
	TcCP14.6-R	GAATTCCTGGTCGGTCCCTG
	TcLCPA3A-F	AAGGCAGCTACTCCCTCACT
	TcLCPA3A-R	GGACAACAGCGTTGAAACCG
	TcRPS18-F	CGAAGAGGTCGAGAAAATCG
	TcRPS18-R	CGTGGTCTTGGTGTGTTGAC
	TcRPL13α-F	ACCATATGACCGCAGGAAAC
	TcRPL13α-R	GGTGAATGGAGCCACTTGTT
dsRNA引物 Primers for dsRNA	dsTcCP14.6-F	ggatcctaatacgactcactataggCACCTCTCATCTCCGATGCT
	dsTcCP14.6-R	ggatcctaatacgactcactataggTTTCACTTCGCCGATCTCCT
	dsTcLCPA3A-F	ggatcctaatacgactcactataggACGAGCAACAATGGCATTCA
	dsTcLCPA3A-R	ggatcctaatacgactcactataggTATTCAACAGTACGGCGGGT
	dsGFP-F	ggatcctaatacgactcactataggATGGTGAGCAAGGGCGAGA
	dsGFP-R	ggatcctaatacgactcactataggTTACTTGTACAGCTCGTCCA

种群 Population	采集地 Collection site	回归方程 <BOLD>R</BOLD>egression equation (y=)	致死中浓度LC₅₀ (μg·L^-1) (95% CI^a)	抗性倍数 RR
JS	江苏Jiangsu	-7.108+6.009x	15.2 (14.1-16.2)	1.7
YN	云南Yunnan	-10.565+7.465x	27.2 (26.7-27.5)	3.0
HN	湖南Hunan	-6.378+2.822x	182.1 (151.6-240.7)	20.2
SC	四川Sichuan	-18.084+5.092x	3558.4 (3402.7-3726.3)	395.4
GD	广东Guangdong	-47.249+12.146x	7763.9 (7401.6-8143.6)	862.7

Cuticle Protein Genes TcCP14.6 and TcLCPA3A are Involved in Phosphine Resistance of Tribolium castaneum

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