Strawberry (Fragaria×ananassa), known as ‘the queen of fruits’, is very popular with consumers due to its flavor and nutritional value. WRKY transcription factors play a critical role in biotic and abiotic stress responses in plants. Based on the cultivated strawberry ‘Camarosa’ genome, 222 FaWRKY genes were identified and analyzed. Meanwhile, FaWRKY genes participate in regulating the growth and development of different tissues and fruit ripening stages. In addition, FaWRKY expression profiles were also studied in the strawberry seedlings inoculated with Colletotrichumfructicola, a major pathogen of strawberry. The photo shows the fruits of the new strawberry cultivar ‘Shenxin’, which was provided by Dr. Zou Xiaohua from Shanghai Academy of Agricultural Sciences, China. See pages 1658–1672 for more details.