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Special Issue:
园艺-分子生物合辑Horticulture — Genetics · Breeding
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| Genome-wide characterization and expression analysis of WRKY
family genes during development and resistance to Colletotrichum
fructicola in cultivated strawberry (Fragaria×ananassa Duch.) |
| ZOU Xiao-hua, DONG Chao, LIU Hai-ting, GAO Qing-hua |
Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai 201403, P.R.China
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摘要
该研究根据2019年公布的栽培草莓“Camarosa”全基因组信息，在“Camarosa”基因组中鉴定出了222个FaWRKY基因，并均匀分布在7条染色体上。通过系统发育分析表明，222个FaWRKY候选基因分为3组，其中41个在Ⅰ组，142个在Ⅱ组，39个在Ⅲ组。比较不同栽培草莓基因组中WRKY基因发现，WRKY基因外显子-内含子结构和基序在不同栽培草莓基因组中具有进化多样性。此外，通过实时荧光定量PCR分析发现FaWRKY133在草莓叶片中表达相对较高；FaWRKY63在根中特异表达。而且，FaWRKY在果实发育不同阶段的草莓种子中有特异性的积累，其中FaWRKY1, 7, 8, 14, 25, 26, 34, 36, 41, 43和45在成熟果实的种子中特异性表达。进一步发现FaWRKY179和205的表达量在浸染草莓炭疽病菌C. fructicola之后不同时期均显著性下调。此研究表明，不同栽培草莓基因组中WRKY基因具有进化多样性，其中栽培草莓 “Camarosa”中的FaWRKY基因表达具有组织特异性和果实发育特异性，而且FaWRKY基因的表达也显著性响应草莓炭疽菌C. fructicola的侵染。这对草莓FaWRKY基因的功能研究提供了新的理论基础。
Abstract Based on the recently published whole-genome sequence of cultivated strawberry ‘Camarosa’, in this study, 222 FaWRKY genes were identified in the ‘Camarosa’ genome. Phylogenetic analysis showed that the 222 FaWRKY candidate genes were classified into three groups, of which 41 were in group I, 142 were in group II, and 39 were in group III. The 222 FaWRKY genes were evenly distributed among the seven chromosomes. The exon–intron structures and motifs of the WRKY genes had evolutionary diversity in different cultivated strawberry genomes. Regarding differential expression, the expression of FaWRKY133 was relatively high in leaves, while FaWRKY63 was specifically expressed in roots. FaWRKY207, 59, 46, 182, 156, 58, 39, 62 and 115 were up-regulated during achene development from the green to red fruit transition. FaWRK181, 166 and 211 were highly expressed in receptacles at the ripe fruit stage. One interesting finding was that FaWRKY179 and 205 were significantly repressed after Colletotrichum fructicola inoculation in both ‘Benihoppe’ and ‘Sweet Charlie’ compared with Mock. The data reported here provide a foundation for further comparative genomics and analyses of the distinct expression patterns of FaWRKY genes in various tissues and in response to C. fructicola inoculation.
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Received: 01 February 2021
Accepted: 23 August 2021
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| Fund:
This work was supported by the National Natural Science Foundation of China (31601731).
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| About author: ZOU Xiao-hua, E-mail: zouxh_113@126.com; Correspondence GAO Qing-hua, E-mail: qhgao20338@sina.com |
Cite this article:
ZOU Xiao-hua, DONG Chao, LIU Hai-ting, GAO Qing-hua.
2022.
Genome-wide characterization and expression analysis of WRKY
family genes during development and resistance to Colletotrichum
fructicola in cultivated strawberry (Fragaria×ananassa Duch.). Journal of Integrative Agriculture, 21(6): 1658-1672.
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