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Special Issue:
棉花合辑Cotton
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| The GhMAX2 gene regulates plant growth and fiber development in cotton |
HE Peng1, ZHANG Hui-zhi1, ZHANG Li1, JIANG Bin1, XIAO Guang-hui1, 2, YU Jia-ning1, 2
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1 College of Life Sciences, Shaanxi Normal University, Xi’an 710119, P.R.China
2 National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, Shaanxi Normal University, Xi’an 710119, P.R.China |
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摘要
本研究鉴定出棉花SLs信号传导途径基因GhMAX2，研究了其在陆地棉生长和株型形成中的作用。生物信息学分析表明GhMAX2蛋白主要由一个α-螺旋和一个无规卷曲组成，含有大量富含亮氨酸的重复序列。GhMAX2基因在根、茎、花和20天的纤维中高表达，GhMAX2启动子驱动的GUS在拟南芥根、主花序、花和角果中表达。亚细胞定位结果显示GhMAX2定位于细胞核。在拟南芥max2-1突变体中异源表达GhMAX2，可以回复至野生型的表型，表明MAX2的功能在陆地棉和拟南芥之间高度保守。此外，棉花中GhMAX2基因的敲低表达可导致植株高度降低，生长缓慢，节间缩短，纤维变短。上述结果表明，GhMAX2可能在棉花的生长、株型形成以及纤维伸长中发挥重要功能。综上，本研究主要揭示了GhMAX2介导的SL/KAR信号在棉花中的功能，为今后棉花新品种培育提供了理论依据。
Abstract
Strigolactones (SLs) are a new type of plant endogenous hormones that have been found to regulate plant growth and architecture. At present, some genes related to the biosynthesis and signaling pathway of SLs have been isolated in plants such as Arabidopsis thaliana, Pisum sativum and Oryza sativa. However, the signaling pathway and specific mechanism of SLs in cotton remain unclear. In this study, we identified the SLs signaling gene GhMAX2 and demonstrated its function in plant growth and architecture in Gossypium hirsutum. Bioinformatics analysis showed that GhMAX2 mainly consists of an α-helix and a random coil and includes a large number of leucine-rich repeats. GhMAX2 was highly expressed in root, stem, flower, and fibers at 20 days post-anthesis (DPA). GhMAX2 promoter-driven β-glucuronidase expression was present exclusively in the root, main inflorescence, flower, and silique. Subcellular localization showed that GhMAX2 is targeted to the nucleus. Heterologously expressed GhMAX2 can rescue the phenotype of Arabidopsis max2-1 mutant, indicating that the function of MAX2 is highly conserved between G. hirsutum and A. thaliana species. In addition, the knockdown expression of GhMAX2 in cotton resulted in significantly reduced plant height, slow growth, short internodes, and reduced fiber length. These findings indicate that GhMAX2 probably contributes to plant growth, architecture and fiber elongation in cotton. The study reveals insights into the roles of GhMAX2-mediated SL/KAR signaling in cotton and provides a valuable foundation for the cultivation of cotton plants in the future.
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Received: 28 September 2020
Accepted: 18 December 2020
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| Fund: This work was supported by the National Natural Science Foundation of China (32000146 and 31470295), the Natural Science Basic Research Program of Shaanxi, China (2018JZ3006, 2019JQ-062 and 2020JQ-410), the Fundamental Research Funds for Central Universities, China (GK201901004 and GK202103063 and GK202002005), the China Postdoctoral Science Foundation (2020T130394), the Young Elite Scientists Sponsorship Program by China Association for Science and Technology (2019-2021QNRC001), and the State Key Laboratory of Cotton Biology Open Fund, China (CB2020A12). |
| About author: Correspondence XIAO Guang-hui, E-mail: guanghuixiao@snnu.edu.cn; YU Jia-ning, E-mail: jnyu@snnu.edu.cn |
Cite this article:
HE Peng, ZHANG Hui-zhi, ZHANG Li, JIANG Bin, XIAO Guang-hui, YU Jia-ning.
2022.
The GhMAX2 gene regulates plant growth and fiber development in cotton. Journal of Integrative Agriculture, 21(6): 1563-1575.
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