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    Salivary protein NlSP6935 that restricted to rice planthoppers is critical for insect survival and host defense regulation
    Zelong Zhang, Xiaojing Wang, Xinye Xu, Tangbin Hu, Chuanxi Zhang, Haijian Huang
    2026, 25 (9): 3762-3774.   DOI: 10.1016/j.jia.2026.02.013
    Abstract71)      PDF in ScienceDirect      

    Saliva plays a crucial role in mediating plant–insect interactions, yet the functional diversity of salivary proteins remains poorly understood.  Here, we identify NlSP6935, a salivary gland-specific protein conserved among rice planthoppers but absent in bamboo-feeding relatives.  Silencing NlSP6935 causes severe lethality, feeding impairment, and infertility in Nilaparvata lugens, independent of host plant resistance.  Transient expression assays reveal that NlSP6935 suppresses H2O2 accumulation in plants, while overexpression in rice downregulates terpenoid biosynthesis and enhances host attractiveness.  However, transgenic NlSP6935 plants only weakly rescue RNAi-induced lethality, demonstrating its dual role in insect physiology and plant defense suppression.  Our findings reveal a novel effector essential for both planthopper survival and host adaptation, providing new insights into pest control strategies.

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    Genetic diversity and recombination analysis of NADC34-like porcine reproductive and respiratory syndrome viruses
    Zhengqin Ye, Wenqiang Wang, Zhenbang Zhu, Wei Wen, Hu Suk Lee, Xiangdong Li
    2026, 25 (8): 3503-3507.   DOI: 10.1016/j.jia.2026.01.006
    Abstract150)      PDF in ScienceDirect      
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    From elite germplasm to transformation platform: Breaking recalcitrance in Tartary buckwheat
    Zhen Wang, Tong Su, Kaixuan Zhang, Yuqi He, Zhirong Wang, Alexander Betekhtin, Meiliang Zhou
    2026, 25 (7): 3090-3093.   DOI: 10.1016/j.jia.2026.03.022
    Abstract151)      PDF in ScienceDirect      
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    Editorial — Digitalization of smallholder agriculture: Adoption and impacts
    Zhanli Sun, Hang Xiong, Hongmei Yi, Lena Kuhn
    2026, 25 (6): 2183-2187.   DOI: 10.1016/j.jia.2026.04.021
    Abstract234)      PDF in ScienceDirect      

    Digital technologies are considered to hold transformative potential for agriculture by enhancing productivity, reducing environmental impacts, improving market access, and strengthening farmer livelihoods (Trendov et al. 2019; Klerkx et al. 2019; Prause et al. 2021; Huang et al. 2023). Mobile phones can provide real-time weather information and market prices, reducing information asymmetries that have long disadvantaged small-scale producers (Aker and Fafchamps 2014). Precision agriculture technologies, including drones and sensor systems, can optimize input use and reduce costs per unit of output (Wolfert et al. 2017). Digital financial services can improve access to credit and insurance, helping farmers manage risks and invest in productivity-enhancing technologies (Suri and Jack 2016). E-commerce platforms may connect smallholders directly to consumers and processors, potentially capturing higher value and margins for producers by bypassing intermediaries (Dannenberg and Lakes 2013; Feng 2024) .

    Meanwhile, smallholder farmers, while playing a critical role in ensuring food security, face distinct disadvantages in accessing and adopting digital technologies as most of them do not reach the critical operational size beyond which technology becomes profitable. High upfront costs of digital devices, connectivity and capacity building remain prohibitive for many resource-constrained households (Fabregas et al. 2019). Furthermore, digital interfaces and technical requirements often exceed the educational backgrounds and technical skills of smallholder farmers (Nakasone and Torero, 2016). Risk aversion, common among vulnerable farming households, can further discourage adoption of unfamiliar technologies (Foster and Rosenzweig 2010). These barriers contribute to a growing digital divide in agriculture, where the benefits of technological advancement increasingly accrue to larger, better-resourced farms while smallholders remain excluded (Jouanjean et al. 2017).

    China, being both the largest producer and consumer of agricultural products worldwide, features an agricultural sector that traditionally was characterized by high input of manual labor and agrochemicals, but low input of technology. This structure can be considered a heritage of ample rural labor on the one hand and lack of private rural finances on the other hand. For millennia, agriculture served as major source of income for China’s rural population. During the shift from a largely agricultural society to a modern, industrially-dominated society, agriculture remained a fall-back option and essentially a social security for the rural population. Currently though, demographic change (i.e., shrinking and rapidly aging population), deterioration and scarcity of natural resources (i.e., soil, water resources) as well as strategic concerns over food security have driven a fundamental need to modernize China’s agricultural sector.

    China is currently aiming to modernize farming by expanding rural digital infrastructure, integrating IT and AI into production, strengthening big-data platforms, and upgrading rural industries. With a series of policies, beginning with the Opinions of the Ministry of Agriculture on Promoting the Development of Agricultural and Rural Big Data (2015) and the Internet Plus Agriculture Plan (2016), China laid the groundwork for spreading digital infrastructure, e-commerce, and precision agriculture. More recent frameworks - including the Smart Agriculture Development Plan, the Development Plan for Digital Agriculturand Rural Areas (2019–2025), the National Smart Agriculture Action Plan (2024–2028), and the Digital Village Strategy - set concrete targets for national data platforms, digital-skills training, and integrated rural digital ecosystems. The newly unveiled China’s 15th Five-Year Plan (2026–2030) further emphasizes accelerating smart agriculture and digitalization technologies (e.g., AI, Drones, and IoT) to achieve food security, agricultural modernization, and rural revitalization. Together, these policies create a comprehensive roadmap for accelerating digital transformation across China’s agri-food sector, and demonstrate the determination of the Chinese government in promoting digitalization in agrifood systems. Compared to other countries, where smallholder-systems are dominating agricultural production, China is leading in digitalization efforts and could offer valuable experiences towards a successful and sustainable digital transformation of the agricultural sectors.

    At the same time, the current political framework has yet to achieve successful incorporation of smallholders into the digitalization process. Empirical evidence showed that family farms in China remain mostly excluded from digitalization processes at present. The digitalization level of most family farms, particularly on the production stage, is still low in China. Considering the barriers to smallholder digitalization laid out earlier, an integration of these producers will likely require active policy support. Otherwise, digitalization is likely to act as a catalyst to already ongoing structural change, crowding out smallholders from commercial farming within a matter of years. While farm consolidation processes worldwide since the 1960s showed that agricultural labor can mostly be absorbed by industrial and service sectors, the social consequences of a high-speed, unchanneled sector transformation will be difficult to predict. To direct policymaking towards not just supporting but also guiding this digitalization process in an inclusive fashion, current smallholder-specific empirical evidence into the conditions, drivers and impacts of digitalization are of high need.

    General research established that in general, high upfront costs and investment risks remain substantial barriers for digital innovation adoption among smallholders (Pivoto et al. 2019; Geng and Liao 2024). Beyond fundamental economic incentives, social and behavioral factors play a critical role, with younger, more educated farmers and those with strong social networks or frequent extension contact demonstrating higher adoption intensity (Pivoto et al. 2019; DeLay et al. 2021; FAO 2022). From a technological perspective, ease of use and robust digital infrastructure - such as reliable internet and technical support - are essential enablers, with “embodied-knowledge” technologies like automated guidance seeing faster diffusion than complex information-intensive systems (Ofori et al. 2020; Damasceno et al. 2025). Furthermore, institutional support through government subsidies and targeted policy interventions effectively mitigates financial constraints, whereas environmental motivations currently appear as secondary or inconclusive drivers compared to direct economic benefits (Geng and Liao 2024; Ruslan 2024; Tian et al. 2025).

    Education and digital literacy emerge as critical factors to reduce adoption costs among smallholders and act as prerequisite for their engagement in agricultural digitalization (Li et al. 2025). Digital literacy encompasses not only basic technical skills for using devices and applications, but also the ability to critically evaluate information, adapt digital tools to local contexts, and integrate digital resources into decision-making processes (Salemink et al. 2017). Research has shown that farmers with higher digital literacy are more likely to adopt beneficial agricultural technologies, make informed and adaptation production decisions, and achieve better economic outcomes and resilience (Deichmann et al. 2016; Bai et al. 2026). Conversely, limited digital literacy can lead to misuse of digital information, inappropriate technology choices, and potentially harmful agricultural practices (Birner et al. 2021).

    The actual or expected impact of agricultural digitalization under smallholders context is another critical research topic. Literature reviews for the grain sector show that both main motivation and impact of digitalization lies within saving labor and other agricultural inputs at given rates of productivity. Meanwhile, international studies have also documented productivity gains from precision agriculture technologies (Lowenberg-DeBoer and Erickson 2019), improved market outcomes from mobile phone adoption (Jensen 2007), and enhanced financial inclusion through digital payment systems (Suri and Jack 2016). However, research has also identified unintended consequences, including increased input use in some contexts (Fabregas et al. 2019) and growing inequality between adopters and non-adopters (Klerkx et al. 2019). These mixed findings underscore the importance of understanding not just whether digital technologies work, but how and under what conditions they benefit different types of farmers.

    This special focus contributes new empirical evidence to these ongoing discussions, with particular attention to the Chinese context, where rapid digital transformation intersects with the world’s largest population of smallholder farmers. The six papers are organized around three complementary themes: behavioral aspects of innovation adoption (Section 1), aspects of digital knowledge and literacy within innovation adoption (Section 2), followed by the actual and expected impacts of digitalization (Section 3).

    Section 1: Behavioral adoption drivers

    Two papers in this section provide detailed insights into behavioral adoption drivers of agricultural drones, or unmanned aerial vehicles (UAVs), under the smallholder context in China. Although these two papers address the farmers’ adoption of same technologies, i.e., agricultural drones, the authors take distinct approaches and pay different attention on the adoption factors.

    Zhang et al. (2026) strived to provide a specific perspective on behavioral aspect by examining farmers’ preferences for agricultural drone services rather than ownership, an organizational innovation which allows small holders to use drones without purchasing them. Using a discrete choice experiment among rice producers in Hubei Province, they show that most farmers are willing to adopt drone services under collective hiring arrangements. The study reveals that farmers are willing to pay for drone services, with localness of suppliers valued more highly than contractual arrangements. Farmers strongly prefer local suppliers and contractual agreements, indicating that supplier uncertainty is a major concern in service-based adoption. These findings suggest that service-based models may offer more inclusive pathways for smallholder access to advanced digital technologies, but require careful attention to trust, reliability, local capacity building, and more importantly, the standardization of the services.

    Zhou et al. (2026) examined the drivers and barriers to UAV adoption among rice farmers in Jiangxi Province, China, using a structural equation model grounded in the Technology Acceptance Model. The study reveals that perceived usefulness and ease of use strongly predict adoption intention, while perceived risk acts as a significant barrier. Importantly, the paper identifies network externalities as a key social factor, with peer influence amplifying adoption likelihood by reducing the perceived risk of innovation adoption. As expected, farm size matters significantly, with larger farms showing higher adoption rates. With their research, the authors provide novel evidence towards the social and psychological process shaped by perceptions, peer effects, and risk considerations behind drone adoption among Chinese smallholders.

    Section 2: Digital literacy as adoption driver

    Beyond economic and behavioral drivers, digital knowledge and capabilities emerge as a cross-cutting theme throughout this special focus, warranting dedicated attention. Two papers in this section provide particularly detailed insights into how digital knowledge shapes technology valuation, information use, and agricultural production behaviors. Collectively, these two articles suggest that digital knowledge and literacy are foundational capabilities that determine whether smallholders can successfully participate in and benefit from agricultural digitalization.

    Bai et al. (2026) focused on climate adaptation, examining whether digital literacy promotes adaptive production behaviors among grain farmers in Sichuan Province, China. The study finds that digital literacy significantly increases adoption of climate-adaptive practices by improving farmers’ perception of climate disaster risks. Importantly, the positive effects of digital literacy are stronger where government support is present, including internet training, climate information services, and agricultural infrastructure development. This paper demonstrates that digital literacy can contribute to agricultural resilience, but requires supportive policy environments to realize its full potential.

    Amolegbe et al. (2026) shifted the focus to Nigeria, Africa, and investigated the relationship between digital technology knowledge and e-commerce valuation among farmers. Using objective measures of digital knowledge rather than self-reported experience, the study reveals significant gaps in basic digital skills despite widespread mobile phone ownership. However, farmers with stronger digital knowledge show substantially higher willingness to pay for digital marketing services. The heterogeneity analysis reveals that digital knowledge benefits vary by age and gender, with young adults and men showing larger increases in e-commerce valuation. These findings underscore that the digital divide extends beyond device access to encompass fundamental differences in capability and knowledge.

    Section 3: Impacts and effects

    While existing policy may support in leveling adoption barriers and provide innovation incentives for smallholders, research may also guide policy makers with respect to how policy may facilitate digital innovation processes towards reducing undesired effects of digitalization and shaping positive benefit incidence among different user groups. Two papers in this special focus address different dimensions of impacts of digitalization: agrochemical use decisions and income stability.

    Liu et al. (2026) focused on the socioeconomic perspective by investigating how digital technology use affects income stability of marginalized farm households. Using panel data and multiple estimation approaches, the study shows that digital technology use significantly improves both income levels and income stability among relocated households participating in China’s poverty alleviation programs, with stronger effects at higher levels of use. The analysis identifies information acquisition and human capital accumulation as key mechanisms, showing that digital technology helps households access employment opportunities and develop skills that contribute to stable livelihoods. The paper also reveals that digital technology is particularly effective at stabilizing income for households experiencing downward volatility, suggesting an important insurance-like function.

    Hu et al. (2026), on the other hand, provided a sobering assessment of how online agricultural information affects input use decisions among Chinese farmers. Using propensity score matching with data from 1,833 farms across five provinces, the study finds that online information use increases rather than decreases chemical fertilizer expenditure, particularly among smallholders. This counterintuitive finding highlights potential problems with the quality and targeting of existing digital agricultural information. The authors suggest that much online agricultural content originates from input suppliers with commercial interests in high application rates, and that smallholders may lack the digital literacy needed to critically evaluate such information. This paper serves as an important reminder that digitalization is not automatically beneficial and that information quality and farmer capability are crucial mediating factors.

    Concluding remarks

    The digitalization of smallholder agriculture represents both an opportunity and a challenge. The papers in this special focus show that digital technologies can indeed contribute to more productive, resilient, and inclusive agricultural systems. However, they also demonstrate that realizing this potential requires careful attention to farmer capabilities, information quality, service delivery models, and supportive policy environments. As the digital transformation of agriculture continues, ensuring that its benefits reach smallholder farmers will require sustained effort across multiple domains of policy and practice.

    Future research should continue to examine these complex relationships between digital technologies, farmer capabilities, and agricultural outcomes. Particular attention should be paid to long-term impacts, inequality effects, and the institutional conditions that enable inclusive digitalization. Mixed-method approaches that combine quantitative impact assessment with qualitative investigation of farmer experiences and institutional dynamics would be especially valuable. Further research into processes, trends and projections of digitalization therefore remains of urgent need.

    Special care should be given to the interaction between digitalization, structural change and sustainability. Providing a critical advantage to large producers with respect to total factor productivity, digitalization is likely to accelerate structural change. By shifting the production function and the efficient scale of production to a level where smallholders are no longer competitive, commercial smallholder production is critically endangered. While a structural change within China’s smallholder farming system is both needed and ultimately inevitable, the challenges lie in ensuring the meaningful engagement of small and disadvantaged farmers and guiding the sustainable transformation of farming system towards digitalization and sustainability.

    This special focus provides a glimpse into the dynamic and emerging field of agricultural digitalization in smallholder contexts, which represents both a compelling scientific frontier and an urgent challenge for sustainable agricultural transformation and global food security. The featured papers collectively offer robust empirical evidence on the heterogeneous effects of digital technologies across different farmer types, technologies, and contexts, moving beyond simple adoption narratives to examine nuanced patterns of use and impact. Additionally, the contributions also shed light on the critical but often overlooked role of digital literacy and information quality in determining whether digitalization benefits or potentially harms smallholder farmers. By demonstrating that digital technologies can both enhance and undermine agricultural outcomes - depending on their design, delivery, and use - this collection underscores the need for more sophisticated approaches that prioritize farmer capabilities alongside technological advancement. We envision this special focus will stimulate further research on agricultural digitalization in smallholder contexts in China and beyond.

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    Green agriculture enabled by versatile metal-organic frameworks: A review
    Lianjie Wan, Fei Ma, Jianmin Zhou, Changwen Du
    2026, 25 (5): 1788-1812.   DOI: 10.1016/j.jia.2026.01.007
    Abstract166)      PDF in ScienceDirect      
    Modern agriculture faces unprecedented challenges: A growing global population, limited arable land, freshwater scarcity, and inefficient agrochemical use have triggered severe environmental degradation.  Pollutants including pesticides, heavy metals, microplastics, antibiotics, nutrient runoff, and greenhouse gases threaten ecosystem stability, food security, and human health.  Metal-organic frameworks (MOFs), with their tunable structures, high porosity, and versatile functionality, emerge as promising materials to address these issues.  This review comprehensively summarizes recent advances in MOFs-based solutions for agriculture.  It covers green synthesis strategies to enhance structural stability and promote circular economy principles.  Applications span three primary domains: pollutant remediation, sustainable technologies (e.g., atmospheric water harvesting, seawater desalination, and green ammonia synthesis), and smart agricultural systems.  The latter enables controlled agrochemical release and real-time sensing and monitoring.  Finally, challenges - such as high costs, biosafety concerns, and scalability limitations - are discussed, alongside forward-looking perspectives including AI-assisted design, improved recyclability, scalable production, and multifunctional integration toward green and smart agriculture.
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    Activity of fungicide cyclobutrifluram against Fusarium fujikuroi and mechanism of the pathogen resistance associated with point mutations in FfSdhB, FfSdhC2 and FfSdhD
    Yang Sun, Yu Liu, Li Zhou, Xinyan Liu, Kun Wang, Xing Chen, Chuanqing Zhang, Yu Chen
    2025, 24 (9): 3511-3528.   DOI: 10.1016/j.jia.2024.01.004
    Abstract601)      PDF in ScienceDirect      
    Rice bakanae disease (RBD) is a devastating plant disease caused by Fusarium fujikuroi. This study aimed to evaluate the potential of cyclobutrifluram, a novel succinate dehydrogenase inhibitor (SDHI), to control RBD, and determine the risk and mechanism of resistance to cyclobutrifluram in F. fujikuroi. In vitro experiments showed that cyclobutrifluram significantly inhibited mycelial growth and spore germination, and altered the morphology of mycelia and conidia. Treatment with cyclobutrifluram significantly decreased mycotoxin production and increased cell membrane permeability in F. fujikuroi. The baseline sensitivity of 72 F. fujikuroi isolates to cyclobutrifluram was determined using mycelial growth and spore germination inhibition assays, which revealed EC50 values of 0.0114 – 0.1304 μg mL-1 and 0.0012 – 0.016 μg mL-1, with mean EC50 values of 0.0410 ± 0.0470 μg mL-1 and 0.0038 ± 0.0015 μg mL-1, respectively. Pot experiments demonstrated that the protective effect of cyclobutrifluram against F. fujikuroi was more significant than that of phenamacril and azoxystrobin, indicating that cyclobutrifluram is a promising antifungal agent for the control of RBD. Six cyclobutrifluram-resistant mutants of F. fujikuroi were obtained via fungicide adaptation. Moreover, these mutants exhibited weaker fitness than their parental isolate and positive cross-resistance with other SDHI fungicides, including pydiflumetofen and penflufen; however, no cross-resistance was detected with other classes of fungicides, including phenamacril, fludioxonil, prochloraz, or azoxystrobin. These results indicated that the resistance risk of F. fujikuroi to cyclobutrifluram might be moderate. Sequencing analysis revealed that mutations, including H248D in FfSdhB, A83V in FfSdhC2, and S106F and E166K in FfSdhD, contributed to resistance, which was confirmed by molecular docking and homologous replacement experiments. The results suggest a high potential for cyclobutrifluram to control RBD and a moderate resistance risk of F. fujikuroi to cyclobutrifluram, which are meaningful findings for the scientific application of cyclobutrifluram.
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    The CONSTANS-LIKE SlCOL1 in tomato regulates the fruit chlorophyll content by stabilizing the GOLDEN2-LIKE protein
    Long Cui, Fangyan Zheng, Chenhui Zhang, Sunan Gao, Jie Ye, Yuyang Zhang, Taotao Wang, Zonglie Hong, Zhibiao Ye, Junhong Zhang
    2025, 24 (2): 536-545.   DOI: 10.1016/j.jia.2024.11.022
    Abstract352)      PDF in ScienceDirect      
    CONSTANS (CO) and CONSTANS-LIKE (COL) transcription factors are known to regulate a series of cellular processes, including the transition from vegetative growth to flower development in plants.  However, their role in regulating the fruit chlorophyll content is poorly understood.  In this study, SlCOL1, the tomato (Solanum lycopersicum) ortholog of Arabidopsis CONSTANS, was shown to play key roles in controlling fruit chlorophyll.  The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit, while the overexpression of SlCOL1 increased it.  An analysis of protein–protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE (GLK2), which promotes the stability of its protein.  The overexpression of SlCOL1 in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit, which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.  These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.


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    Lysobacter enzymogenes: A fully armed biocontrol warrior
    Long Lin, Xiaolong Shao, Yicheng Yang, Aprodisia Kavutu Murero, Limin Wang, Gaoge Xu, Yangyang Zhao, Sen Han, Zhenhe Su, Kangwen Xu, Mingming Yang, Jinxing Liao, Kaihuai Li, Fengquan Liu, Guoliang Qian
    2025, 24 (1): 23-35.   DOI: 10.1016/j.jia.2024.02.021
    Abstract568)      PDF in ScienceDirect      
    Lysobacter enzymogenes is less-studied, but emerging as a powerful biocontrol bacterium producing multiple antimicrobial weapons including lytic enzymes, toxins, secondary metabolites and protein secretion systems.  The loss of surface-attached flagellum, production of heat-stable antifungal factor (HSAF, also named as Ningrongmycin) as a novel antifungal antibiotic, and the use of the type IV secretion system (T4SS) rather than the common type VI secretion system (T6SS) to kill competitor bacteria make this species unique.  These distinct features set Lenzymogenes apart from well-studied plant beneficial biocontrol agents, such as Bacillus and Pseudomonas.  This review describes what takes Lenzymogenes to be a unique biocontrol warrior by focusing to illustrate how the lack of flagellum governs morphological and functional co-adaptability, what adapted signaling transduction pathways are adopted to coordinate the biosynthesis of HSAF, and how to ecologically adapt plant rhizosphere by cell-to-cell interacting with microbiome members via the bacterial-killing T4SS.


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    Optimizing crop yields while minimizing environmental impact through deep placement of nitrogen fertilizer
    Lingxiao Zhu, Hongchun Sun, Liantao Liu, Ke Zhang, Yongjiang Zhang, Anchang Li, Zhiying Bai, Guiyan Wang, Xiaoqing Liu, Hezhong Dong, Cundong Li
    2025, 24 (1): 36-60.   DOI: 10.1016/j.jia.2024.05.012
    Abstract381)      PDF in ScienceDirect      
    Nitrogen (N) serves as an essential nutrient for yield formation across diverse crop types.  However, agricultural production encounters numerous challenges, notably high N fertilizer rates coupled with low N use efficiency and serious environmental pollution.  Deep placement of nitrogen fertilizer (DPNF) is an agronomic measure that shows promise in addressing these issues.  This review aims to offer a comprehensive understanding of DPNF, beginning with a succinct overview of its development and methodologies for implementation.  Subsequently, the optimal fertilization depth and influencing factors for different crops are analyzed and discussed.  Additionally, it investigates the regulation and mechanism underlying the DPNF on crop development, yield, N use efficiency and greenhouse gas emissions.  Finally, the review delineates the limitations and challenges of this technology and provides suggestions for its improvement and application.  This review provides valuable insight and reference for the promotion and adoption of DPNF in agricultural practice.
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    Elucidation of the structure, antioxidant, and interfacial properties of flaxseed proteins tailored by microwave treatment
    YU Xiao, DUAN Zi-qiang, QIN Xiao-peng, ZHU Ying-ying, HUANG Feng-hong, PENG Deng-feng, BAI Yan-hong, DENG Qian-chun
    2023, 22 (5): 1574-1589.   DOI: 10.1016/j.jia.2023.04.021
    Abstract554)      PDF in ScienceDirect      
    The microwave treatment is commonly applied to flaxseed to release nutrients, inactivate enzymes, remove cyanogens, and intensify flavors. The current study aimed to explore the influences of microwave exposure on the antioxidant and interfacial properties of flaxseed protein isolates (FPI), focusing on the altering composition and molecular structure. The results showed that after microwave exposure (700 W, 1–5 min), more compact assembly of storage proteins and subsequent permeation by membrane fragments of oil bodies occurred for cold-pressing flaxseed flours. Moreover, the particle sizes of FPI was progressively reduced with the decrement ranged from 37.84 to 60.66% , whereas the zeta potential values initially decreased and then substantially recovered during 1–5 min of microwave exposure. The conformation unfolding, chain cross-linking, and depolymerization were sequentially induced for FPI based on the analysis of fluorescence emission spectra, secondary structure, and protein subunit profiles, thereby affecting the dispersion or aggregation properties between albumin and globulin fractions in FPI. Microwave exposure retained specific phenolic acids and superior antioxidant activities of FPI. The inferior gas–water interface absorption and the loose/porous assembly structure were observed for the foams prepared by FPI, concurrent with obviously shrinking foaming properties upon microwave exposure. Improving oil–water interface activities of FPI produced the emulsion droplets with descending sizes and dense interface coating, which were then mildly destabilized due to the lipid leakage and weakened rheological behavior with microwave exposure extended to 5 min. Our findings elucidated that microwave treatment could tailor the application functionality of protein fractions in flaxseed based on their structural remodeling.
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    Multi-omics-driven development of alternative crops for natural rubber production
    YANG Ning, YANG Dan-dan, YU Xu-chen, XU Cao
    2023, 22 (4): 959-971.   DOI: 10.1016/j.jia.2023.03.007
    Abstract618)      PDF in ScienceDirect      

    Natural rubber (NR) is an irreplaceable biopolymer of economic and strategic importance owing to its unique physical and chemical properties.  The Pará rubber tree (Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.) is currently the exclusive commercial source of NR, and it is primarily grown in plantations restricted to the tropical and subtropical areas of Southeast Asia.  However, current Pará rubber production barely meets the sharply increasing global industrial demand for rubber.  Petroleum-based synthetic rubber (SR) has been used to supplement the shortage of NR but its industrial performance is not comparable to that of NR.  Thus, there is an urgent need to develop new productive rubber crops with broader environmental adaptability.  This review summarizes the current research progress on alternative rubber-producing plants, including horticultural plants (Taraxacum kok-saghyz Rodin and Lactuca L. species), woody plants (Parthenium argentatum A. Gray and Eucommia ulmoides Oliv.), and other plant species with potential for NR production.  With an emphasis on the molecular basis of NR biosynthesis revealed by a multi-omics approach, we highlight new integrative strategies and biotechnologies for exploring the mechanism of NR biosynthesis with a broader scope, which may accelerate the breeding and improvement of new rubber crops. 

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    Potato late blight caused by Phytophthora infestans: From molecular interactions to integrated management strategies
    DONG Suo-meng, ZHOU Shao-qun
    2022, 21 (12): 3456-3466.   DOI: 10.1016/j.jia.2022.08.060
    Abstract873)      PDF in ScienceDirect      

    Over 170 years after the infamous Irish Potato Famine, potato late blight (PLB) caused by Phytophthora infestans remains the single most devastating disease of global potato production, causing up to 10 billion USD in yield loss and management costs.  Through decades of research, growers and agronomists in the field as well as laboratory scientists have made significant progress in understanding the molecular pathogenesis process of this critical pathosystem and effective management strategies to control PLB.  Yet, the need to feed an ever-increasing global population under changing climate demands continued improvement in efficient and sustainable PLB management schemes that can be implemented across a broad economic spectrum.  In this review, we briefly summarize the current understanding of the molecular interaction between P. infestans and its host plants, highlight the current integrated pest management strategy to control PLB on local and continental scales, and discuss the potential of further improvement of sustainable PLB control through genetic enhancement of crop resistance and emerging crop protection technologies.

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    Pig macrophages with site-specific edited CD163 decrease the susceptibility to infection with porcine reproductive and respiratory syndrome virus
    XU Kui, ZHOU Yan-rong, SHANG Hai-tao, XU Chang-jiang, TAO Ran, HAO Wan-jun, LIU Sha-sha, MU Yu-lian, XIAO Shao-bo, LI Kui
    2023, 22 (7): 2188-2199.   DOI: 10.1016/j.jia.2022.11.010
    Abstract564)      PDF in ScienceDirect      
    Porcine reproductive and respiratory syndrome (PRRS) is recognized as one of the most infectious viral diseases of swine. Although Cluster of differentiation 163 (CD163) is identified as an essential receptor for mediating PRRS virus (PRRSV) infection, the important residues involved in infection on CD163 are still unclear. Therefore, it is very important to identify these key residues to study the mechanism of PRRSV infection and to generate anti-PRRSV pigs. In this study, we first generated immortalized porcine alveolar macrophage (IPAM) cell lines harboring 40-residues (residues 523–562, including R561 (arginine (R) at position 561)) deletion of CD163. PRRSV infection experiments showed that these IPAM cell lines were completely resistant to PRRSV infection. We then generated cloned pigs carrying CD163- R561A (an arginine (R) to alanine (A) substitution at position 561 of CD163). PRRSV challenge experiments in porcine alveolar macrophages (PAMs) isolated from the CD163-R561A pigs showed significantly lower susceptibility to PRRSV than that of CD163-R561 PAMs. Through this study, we show that CD163 523–562 contains essential residues for mediating PRRSV infection, and that CD163 R561 significantly contributes to PRRSV infection but is not essential for infection. These functional sites can therefore serve as new targets for understanding the mechanism of PRRSV infection. Furthermore, CD163-R561A pigs can be used as an important model for improving pig germplasm with resistance against PRRSV.
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    CRISPR-based genetic control strategies for insect pests
    Ying YAN, Roswitha A. AUMANN, Irina HÄCKER, Marc F. SCHETELIG
    2023, 22 (3): 651-668.   DOI: 10.1016/j.jia.2022.11.003
    Abstract435)      PDF in ScienceDirect      

    Genetic control strategies such as the sterile insect technique have successfully fought insect pests worldwide.  The CRISPR (clustered regularly interspaced short palindromic repeats) technology, together with high-quality genomic resources obtained in more and more species, greatly facilitates the development of novel genetic control insect strains that can be used in area-wide and species-specific pest control programs.  Here, we review the research progress towards state-of-art CRISPR-based genetic control strategies, including gene drive, sex ratio distortion, CRISPR-engineered genetic sexing strains, and precision-guided sterile insect technique.  These strategies’ working mechanisms, potential resistance development mechanisms, and regulations are illustrated and discussed.  In addition, recent developments such as stacked and conditional systems are introduced.  We envision that the advances in genetic technology will continue to be one of the driving forces for developing the next generation of pest control strategies.  

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    IPM - Biological and integrated management of desert locust
    LI Shuang, FENG Shi-qian, Hidayat ULLAH, TU Xiong-bing, ZHANG Ze-hua
    2022, 21 (12): 3467-3487.   DOI: 10.1016/j.jia.2022.09.017
    Abstract655)      PDF in ScienceDirect      

    Locusts have caused periodic disasters in the recorded history of humankind.  Up to now, locust disaster is still the biggest threat to the world’s agricultural production.  The desert locust Schistocerca gregaria is one of the most harmful locusts, which has caused massive food crises, economic losses, and ecological disasters.  The desert locust is a migratory insect pest that occurs year-round in the tropic and subtropical regions.  Under the wind and seasonal alternation, it moves and flies in the African continent and West Asia.  Desert locust damages the stems and leaves of more than 300 plants, including Gramineae, Tribulus terrestris, and Euphorbiaceae.  Locusts cause devastating disasters to local plants, especially field crops, and significantly threaten food security.  To date, voluminous research has been conducted regarding the ecology and management of desert locusts.  This review represents an effort to summarize the basic information on the biology and ecology, distribution, damage, and economic impact of desert locusts, examine the recent developments in integrated locust management, and make recommendations for future research.  

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    Effect of high-molecular-weight glutenin subunit Dy10 on wheat dough properties and end-use quality
    WANG Yan, GUO Zhen-ru, CHEN Qing, LI Yang, ZHAO Kan, WAN Yong-fang, Malcolm J. HAWKESFORD, JIANG Yun-feng, KONG Li, PU Zhi-en, DENG Mei, JIANG Qian-tao, LAN Xiu-jin, WANG Ji-rui, CHEN Guo-yue, MA Jian, ZHENG You-liang, WEI Yu-ming, QI Peng-fei
    2023, 22 (6): 1609-1617.   DOI: 10.1016/j.jia.2022.08.041
    Abstract559)      PDF in ScienceDirect      
    High-molecular-weight glutenin subunits (HMW-GSs) are the most critical grain storage proteins that determine the unique processing qualities of wheat. Although it is a part of the superior HMW-GS pair (Dx5+Dy10), the contribution of the Dy10 subunit to wheat processing quality remains unclear. In this study, we elucidated the effect of Dy10 on wheat processing quality by generating and analyzing a deletion mutant (with the Dy10-null allele), and by elucidating the changes to wheat flour following the incorporation of purified Dy10. The Dy10-null allele was transcribed normally, but the Dy10 subunit was lacking. These findings implied that the Dy10-null allele reduced the glutenin:gliadin ratio and negatively affected dough strength (i.e., Zeleny sedimentation value, gluten index, and dough development and stability times) and the bread-making quality; however, it positively affected the biscuit-making quality. The incorporation of various amounts of purified Dy10 into wheat flour had a detrimental effect on biscuit-making quality. The results of this study demonstrate that the Dy10 subunit is essential for maintaining wheat dough strength. Furthermore, the Dy10-null allele may be exploited by soft wheat breeding programs.
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    Maleness-on-the-Y (MoY) orthologue is a key regulator of male sex determination in Zeugodacus cucurbitae (Diptera: Tephritidae)

    FAN Zi-zhen, MA Qin, MA Si-ya, CAO Feng-qin, YAN Ri-hui, LIN Xian-wu
    2023, 22 (2): 505-513.   DOI: 10.1016/j.jia.2022.08.007
    Abstract817)      PDF in ScienceDirect      

    The initiation of sex differentiation in insects is regulated by primary sex determination signals.  In the Medfly Ceratitis capitata and other Tephritids, Maleness-on-the-Y (MoY) is the master gene for male sex determination.  However, the primary signal in Zeugodacus cucurbitae (Coquillett), a very destructive Tephritid pest across the world, remains ambiguous.  In this study, we have isolated and characterized the Medfly MoY homolog in Zcucurbitae, ZcMoY.  ZcMOY protein shows high sequence conservation to its homologs in Bactrocera species.  ZcMoY transcription begins and peaks at very early embryonic stages and then becomes undetectable except the testes and heads of day 1 male adults.  Silencing ZcMoY in early embryos by RNAi causes abnormal external genitalia and interior reproductive organs, giving rise to intersexes and feminization of XY individuals.  The expression pattern and knockdown phenotypes of ZcMoY indicate that ZcMoY plays a key role in regulating sex determination of Zcucurbitae males.  Our findings will help the understanding of sex determination in Zcucurbitae and facilitate the development of genetic sexing strains in its biological control.

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    A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression
    DONG Shi-man, XIAO Liang, LI Zhi-bo, SHEN Jie, YAN Hua-bing, LI Shu-xia, LIAO Wen-bin, PENG Ming
    2022, 21 (9): 2588-2602.   DOI: 10.1016/j.jia.2022.07.022
    Abstract422)      PDF in ScienceDirect      

    Cassava is an important tropical cash crop.  Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production.  Enhancing the drought tolerance of cassava can effectively improve its yield.  Long non-coding RNAs (lncRNAs) are present in a wide variety of eukaryotes.  Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses.  However, the function of cassava lncRNAs in the drought response remains largely unknown.  In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA (DIR).  Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid (ABA) or jasmonic acid (JA) treatments.  In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava.  RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism.  Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins.  A protein–protein interaction (PPI) analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control pathways.  Collectively, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response.  Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

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    Evaluation of the early defoliation trait and identification of resistance genes through a comprehensive transcriptome analysis in pears
    SHAN Yan-fei, LI Meng-yan, WANG Run-ze, LI Xiao-gang, LIN Jing, LI Jia-ming, ZHAO Ke-jiao, WU Jun
    2023, 22 (1): 120-138.   DOI: 10.1016/j.jia.2022.08.040
    Abstract534)      PDF in ScienceDirect      

    Early defoliation, which usually occurs during summer in pear trees, is gradually becoming a major problem that poses a serious threat to the pear industry in southern China.  However, there is no system for evaluating the responses of different cultivars to early defoliation, and our knowledge of the potential molecular regulation of the genes underlying this phenomenon is still limited.  In this study, we conducted field investigations of 155 pear accessions to assess their resistance or susceptibility to early defoliation.  A total of 126 accessions were found to be susceptible to early defoliation, and only 29 accessions were resistant.  Among them, 19 resistant accessions belong to the sand pear species (Pyrus pyrifolia).  To identify the resistance genes related to early defoliation, the healthy and diseased samples of two sand pear accessions, namely, the resistant early defoliation accession ‘Whasan’ and the susceptible early defoliation accession ‘Cuiguan’, were used to perform RNA sequencing.  Compared with ‘Cuiguan’, a total of 444 genes were uniquely differentially expressed in ‘Whasan’.  Combined with GO and KEGG enrichment analyses, we found that early defoliation was closely related to the stress response.  Furthermore, a weighted gene co-expression network analysis revealed a high correlation of WRKY and ethylene responsive factor (ERF) transcription factors with early defoliation resistance.  This study provides useful resistant germplasm resources and new insights into potentially essential genes that respond to early defoliation in pears, which may facilitate a better understanding of the resistance mechanism and molecular breeding of resistant pear cultivars

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    OsPPR9 encodes a DYW-type PPR protein that affects editing efficiency of multiple RNA editing sites and is essential for chloroplast development
    CHEN Chang-zhao, WANG Ya-Liang, HE Meng-xing, LI Zhi-wen, SHEN Lan, LI Qing, RE De-yong, HU Jiang, ZHU Li, ZHANG Guang-heng, GAO Zhen-yu, ZENG Da-li, GUO Long-biao, QIAN Qian, ZHANG Qiang
    2023, 22 (4): 972-980.   DOI: 10.1016/j.jia.2022.08.026
    Abstract572)      PDF in ScienceDirect      

    Photosynthesis occurs mainly in chloroplasts, whose development is regulated by proteins encoded by nuclear genes.  Among them, pentapeptide repeat (PPR) proteins participate in organelle RNA editing.  Although there are more than 450 members of the PPR protein family in rice, only a few affect RNA editing in rice chloroplasts.  Gene editing technology has created new rice germplasm and mutants, which could be used for rice breeding and gene function study.  This study evaluated the functions of OsPPR9 in chloroplast RNA editing in rice.  The osppr9 mutants were obtained by CRISPR/Cas9, which showed yellowing leaves and a lethal phenotype, with suppressed expression of genes associated with chloroplast development and accumulation of photosynthetic-related proteins.  In addition, loss of OsPPR9 protein function reduces the editing efficiency of rps8-C182, rpoC2-C4106, rps14-C80, and ndhB-C611 RNA editing sites, which affects chloroplast growth and development in rice.  Our data showed that OsPPR9 is highly expressed in rice leaves and encodes a DYW-PPR protein localized in chloroplasts.  Besides, the OsPPR9 protein was shown to interact with OsMORF2 and OsMORF9.  Together, our findings provide insights into the role of the PPR protein in regulating chloroplast development in rice. 

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