麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
|A major and stable QTL for wheat spikelet number per spike
validated in different genetic backgrounds
|DING Pu-yang1*, MO Zi-qiang1*, TANG Hua-ping1*, MU Yang1, DENG Mei1, JIANG Qian-tao1, LIU Ya-xi1, CHEN Guang-deng2, CHEN Guo-yue1, WANG Ji-rui1, LI Wei3, QI Peng-fei1, JIANG Yun-feng1, KANG Hou-yang1, YAN Gui-jun4, Wei Yu-ming1, ZHENG You-liang1, LAN Xiu-jin1, MA Jian1
|1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 College of Resources, Sichuan Agricultural University, Chengdu 611130, P.R.China
3 College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R.China
4 University of Western Australia School of Agriculture and Environment and the UWA Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley 6009, Australia
Abstract The spikelet number per spike (SNS) contributes greatly to grain yield in wheat. Identifying various genes that control wheat SNS is vital for yield improvement. This study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci (QTLs) for SNS. One of them (QSns.sau-2SY-2D.1) was reported previously, while the other (QSns.sau-2SY-7A) was newly detected and further analyzed in this study. QSns.sau-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21–40.78% of the phenotypic variances. QSns.sau-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm 7AL. The contributions and interactions of both major QTLs were further analyzed and discussed. The effect of QSns.sau-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F2:3 population and a panel of 101 high-generation breeding wheat lines. Furthermore, several genes including the previously reported WHEAT ORTHOLOG OF APO1 (WAPO1), an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1 (APO1) related to SNS, were predicted in the interval of QSns.sau-2SY-7A. In summary, these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.
Received: 23 September 2020
Accepted: 17 December 2020
|Fund: This work was supported by the projects from the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2020YJ0140 and 2021YJ0503), the International Science and Technology Cooperation and Exchanges Program of Science and Technology Department of Sichuan Province, China (2021YFH0083 and 2022YFH0053), the National Natural Science Foundation of China (31971937 and 31970243), and the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province, China.
|About author: Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350, E-mail: firstname.lastname@example.org; LAN Xiu-jin, E-mail: email@example.com
* These authors contributed equally to this study.
Cite this article:
DING Pu-yang, MO Zi-qiang, TANG Hua-ping, MU Yang, DENG Mei, JIANG Qian-tao, LIU Ya-xi, CHEN Guang-deng, CHEN Guo-yue, WANG Ji-rui, LI Wei, QI Peng-fei, JIANG Yun-feng, KANG Hou-yang, YAN Gui-jun, Wei Yu-ming, ZHENG You-liang, LAN Xiu-jin, MA Jian.
A major and stable QTL for wheat spikelet number per spike
validated in different genetic backgrounds. Journal of Integrative Agriculture, 21(6): 1551-1562.
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