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Journal of Integrative Agriculture  2022, Vol. 21 Issue (6): 1703-1712    DOI: 10.1016/S2095-3119(20)63578-X
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Physiological mitochondrial ROS regulate diapause by enhancing HSP60/Lon complex stability in Helicoverpa armigera
ZHANG Xiao-shuai, SU Xiao-long, GENG Shao-lei, WANG Zheng-hao
School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, P.R.China
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之前研究表明活性氧(reactive oxygen species,ROS)在棉铃虫滞育蛹脑中通过调节独特的胰岛素信号通路转导机制来促进滞育。然而,滞育蛹脑中ROS的来源及调控滞育的机制尚不清楚。本研究的结果显示,滞育蛹脑中积累了高水平的线粒体ROS和总ROS,说明线粒体是滞育蛹脑中ROS的主要来源。另外,注射葡萄糖代谢抑制剂2-脱氧-D-葡萄糖可通过提高非滞育蛹脑中线粒体ROS的水平进而延迟蛹的发育。注射代谢物混合物到滞育蛹中可以降低线粒体ROS的水平进而逆转滞育的进程。进一步的研究显示,线粒体ROS可以激活HSP60的表达和活性,进而促进HSP60/Lon复合物的稳定性,从而降解线粒体转录因子A和降低线粒体活性或生成。因此,本研究阐明了ROS通过降低线粒体活性而促进滞育或寿命延长的有益作用

Diapause is a long-lived stage which has evolved into an important strategy for insects to circumvent extreme environments.  In the pupal stage, Helicoverpa armigera can enter diapause, a state characterized by significantly decreased metabolic activity and enhanced stress resistance, to survive cold winters.  Previous studies have shown that reactive oxygen species (ROS) can promote the diapause process by regulating a distinct insulin signaling pathway.  However, the source of ROS in the diapause-destined pupal brains and mechanisms by which ROS regulate diapause are still unknown.  In this study, we showed that diapause-destined pupal brains accumulated high levels of mitochondrial ROS (mtROS) and total ROS during the diapause process, suggesting that mitochondria are the main source of ROS in diapause-destined pupal brains.  In addition, injection of 2-deoxy-D-glucose (DOG), a glucose metabolism inhibitor, could delay pupal development by elevating mtROS levels in the nondiapause-destined pupal brains.  Furthermore, the injection of a metabolite mixture to increase metabolic activity could avert the diapause process in diapause-destined pupae by decreasing mtROS levels.  We also found that ROS could activate HSP60 expression and promote the stability of the HSP60-Lon complex, increasing its ability to degrade mitochondrial transcription factor A (TFAM) and decreasing mitochondrial activity or biogenesis under oxidative stress.  Thus, this study illustrated the beneficial function of ROS in diapause or lifespan extension by decreasing mitochondrial activity.
Keywords:  mtROS        diapause        HSP60        Helicoverpa armigera  
Received: 30 October 2020   Accepted: 29 November 2020
Fund: This study was supported by the China Postdoctoral Science Foundation (2017M622872).  
About author:  Correspondence ZHANG Xiao-shuai, Tel: +86-20-39332964, Fax: +86-20-39332294, E-mail: zhangxswb@hotmail. com

Cite this article: 

ZHANG Xiao-shuai, SU Xiao-long, GENG Shao-lei, WANG Zheng-hao. 2022. Physiological mitochondrial ROS regulate diapause by enhancing HSP60/Lon complex stability in Helicoverpa armigera. Journal of Integrative Agriculture, 21(6): 1703-1712.

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