|
Special Issue:
园艺-分子生物合辑Horticulture — Genetics · Breeding
|
|
|
|
| Effects of exogenous paclobutrazol and sampling time on the
efficiency of in vitro embryo rescue in the breeding of new seedless
grape varieties |
XU Teng-fei1*, GUO Yu-rui2*, YUAN Xiao-jian1, CHU Yan-nan1, WANG Xiao-wei1, HAN Yu-lei1, WANG Wen-yuan1, WANG Yue-jin1, SONG Rui3, FANG Yu-lin3, WANG Lu-jun4, XU Yan1
|
1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, P.R.China
2 College of Plant Science, Tibet Agricultural and Animal Husbandry University, Nyingchi, Tibet 860000, P.R.China
3 Institute for China Wine Industry Technology, Yinchuan, Ningxia 750000, P.R.China
4 Institute for Weinan Grape Research, Weinan, Shaanxi 714099, P.R.China
|
|
|
|
|
摘要
本研究调查了香味和抗寒无核品种选育中不同浓度的多效唑及胚采集时期对胚形成、萌发和成苗率的影响。结果显示，不同浓度的多效唑对不同葡萄品种子房和胚的发育影响不一致。红无籽露×北冰红和昆香无核×泰山-2组合在1.5 mg L-1多效唑处理下胚形成率最高。红无籽露×北冰红组合在1.0 μmol L-1多效唑处理下萌芽率和成苗率最高，但红无籽露×昆香无核组合在0.2 μmol L-1多效唑处理下萌芽率最好。不同的杂交组合取胚时间也不同。火焰无核×玫瑰香最佳取胚时间为授粉后39 d，昆香无核×北冰红为授粉后46 d，红宝石无核×北冰红及奇妙无核×双优为授粉后41 d。另外，向培养基中补充0.5 mg L-1吲哚丁酸可以帮助畸形苗恢复为正常幼苗并获得生长健壮的子代。研究结果将为利用胚挽救技术选育无核葡萄新品种奠定基础。
Abstract
Embryo rescue technology plays an important role in seedless grape breeding. However, the efficiency of embryo rescue, including the embryo formation, germination, and seedling rates, is closely related to the parental genotypes, degree of abortion, growth medium, and plant growth regulators. In this study, we investigated the effects of different concentrations of paclobutrazol (PAC), a plant growth regulator, and embryo collection times on the embryo formation, germination, and seedling rates for different hybrid combinations of grape breeding varieties used for their aroma and cold-resistance traits. The results showed that the different PAC concentrations had varying impacts on the development of ovules and embryos from the different grape varieties. The embryo formation rates of the ‘Sultanina Rose’בBeibinghong’ and ‘Kunxiang Seedless’בTaishan-2’ crosses were the highest under the 5.1 μmol L–1 PAC treatment. The 1.0 μmol L–1 PAC treatment was optimal for the germination and seedling development of the ‘Sultanina Rose’בBeibinghong’ embryos, whereas the 0.2 μmol L–1 PAC treatment induced the highest germination rate for the ‘Sultanina Rose’בKunxiang Seedless’ cross. The optimal sampling times for each cross varied as 39 d after pollination (DAP) for the ‘Flame Seedless’בMuscat Hamburg’ cross, 46 DAP for the ‘Kunxiang Seedless’בBeibinghong’ cross, and 41 DAP for the ‘Ruby Seedless’בBeibinghong’ and ‘Fantasy Seedless’בShuangyou’ crosses. Moreover, the medium modified with 0.5 g L–1 of indole-3-butyric acid allowed the malformed seedlings to develop into plantlets and achieve larger progenies. This study provides a useful basis for further studies into grape embryo rescue and could improve breeding efforts for new seedless grape varieties.
|
|
Received: 01 February 2021
Accepted: 01 August 2021
|
| Fund:
This research was supported by the Key Industry Chain of Innovation Project in Shaanxi Province, China (2021ZDLNY04–08), the Fundamental Research Funds for the Central Universities, China (2452019016), the China Agriculture Research System of MOF and MARA (CARS–30-yz-7), and the Ningxia Hui Autonomous Region Flexible Introduction of Scientific and Technological Innovation Team, China (2020RXTDLX08).
|
| About author: Correspondence XU Yan, Tel: +86-29-87082522, Fax: +86-29-87082149, E-mail: yan.xu@nwafu.edu.cn
* These authors contributed equally to this study. |
Cite this article:
XU Teng-fei, GUO Yu-rui, YUAN Xiao-jian, CHU Yan-nan, WANG Xiao-wei, HAN Yu-lei, WANG Wen-yuan, WANG Yue-jin, SONG Rui, FANG Yu-lin, WANG Lu-jun, XU Yan.
2022.
Effects of exogenous paclobutrazol and sampling time on the
efficiency of in vitro embryo rescue in the breeding of new seedless
grape varieties. Journal of Integrative Agriculture, 21(6): 1633-1644.
|
Agüero C, Riquelme C, Trio R. 1995. Embryo rescue from seedless grapevines (Vitis vinifera L.) treated with growth retardants. Vitis, 34, 73–76.
Agüero C, Vigliocco A, Abdala G, Tizio R. 2000. Effect of gibberellic acid and uniconazol on embryo abortion in the stenospermocarpic grape cultivars Emperatriz and Perlon. Plant Growth Regulation, 30, 9–16.
Anderson N, Byrne D H, Sinclair J, Burrell A M. 2002. Cooler temperature during germination improves the survival of embryo cultured peach seed. HortScience, 37, 402–403.
Blaikie S J, Kulkarni V J, Müller W J. 2004. Effects of morphactin and paclobutrazol flowering treatments on shoot and root phenology in mango cv. Kensington Pride. Scientia Horticulturae, 101, 51–68.
Cain D W, Emershad R L, Tarailo R E. 1983. In-ovulo embryo culture and seedling development of seeded and seedless prapes (Vitis vinifera L.). Vitis, 22, 9–14.
Chen S, Wang X J, Tan G F, Zhou W Q, Wang G L. 2020. Gibberellin and the plant growth retardant paclobutrazol altered fruit shape and ripening in tomato. Protoplasma, 257, 853–861.
Dantas A C M, Boneti J I, Nodari R O, Guerra M P. 2006. Embryo rescue from interspecific crosses in apple rootstocks. Pesquisa Agropecuária Brasileira, 41, 969–973.
Ebadi A, Sarikhani H, Zamani Z, Babalar M. 2004. Effect of male parent and application of boric acid on embryo rescue in some seedless grapevine (Vitis vinifera) cultivars. Acta Horticulturae, 640, 255–260.
Graham J H, Montague D T, Durham R E, Herring A D. 2002. Root-zone refrigeration delays budbreak and reduces growth of two containerized, greenhouse grown grape cultivars. Texas Journal of Agriculture and Natural Resources, 15, 71–80.
Gray D J, Mortensen J A, Benton C M, Durham R E, Moore G A. 1990. Ovule culture to obtain progeny from hybrid seedless bunch grapes. Journal of the American Society for Horticultural Science, 115, 1019–1024.
Guo Y, Zhao Y, Li K, Liu Z, Lin H, Guo X, Li C. 2011. In vitro embryo rescue culture of F1 progenies from crosses between tetraploid grape and Vitis amurensis. African Journal of Agricultural Research, 6, 4906–4909.
Hajihashemi S, Ehsanpour A A. 2014. Antioxidant response of Stevia rebaudiana B. to polyethylene glycol and paclobutrazol treatments under in vitro culture. Applied Biochemistry and Biotechnology, 172, 4038–4052.
Hajihashemi S, Rajabpoor S, Djalovic I. 2018. Antioxidant potential in Stevia rebaudiana callus in response to polyethylene glycol, paclobutrazol and gibberellin treatments. Physiology and Molecular Biology of Plants, 24, 335–341.
Hao Y, Wang H, Yang R, Wang F L, Lu J. 2010. Effectiveness of culture media on the success of embryo rescue among different crosses of seedless grapes. Acta Horticulturae, 1046, 259–263.
Hu D, Tang X, Zhang Q, Luo Z. 2013. Cross compatibilities of oriental persimmon ‘Mopanshi’ and ‘Luotian-tianshi’ with ‘Zenjimaru’. Acta Horticuturae, 996, 165–170.
Iwahori S, Weaver R J, Pool R M. 1968. Gibberellin-like activity in berries of seeded and seedless Tokay grapes. Plant Physiology, 43, 333–337.
Ji W, Li Z Q, Zhou Q, Yao W K, Wang Y J. 2013. Breeding new seedless grape by means of in vitro embryo rescue. Genetics and Molecular Research, 12, 859–869.
Ji W, Wang Y J. 2013. Breeding for seedless grapes using Chinese wild Vitis spp. II. In vitro embryo rescue and plant development. Journal of the Science of Food and Agriculture, 93, 3870–3875.
Jiao Y T, Li Z Q, Xu K Y, Guo Y R, Zhang C, Li T M, Jiang Y X, Xu Y. 2017. Study on improving plantlet development and embryo germination rates in in vitro embryo rescue of seedless grapevine. New Zealand Journal of Crop and Horticultural Science, 46, 39–53.
Krishna H, Singh S K. 2007. Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement: A review. Biotechnology Advances, 25, 223–243.
Ledbetter C A, Ramming D W. 1989. Seedlessness in grapes. Horticultural Review, 11, 159–184.
Ledbetter C A, Shonnard C B. 1990. Improved seed development and germination of stenospermic grapes by plant growth regulators. Scientia Horticulturae, 65, 269–274.
Leng P, Yamamura H. 2006. Fruit set and embryo rescue in crosses using parthenocarpic ‘Mopanshi’ persimmon. Scientia Horticulturae, 107, 332–336.
Li G R, Ji W, Wang G, Zhang J X, Wang Y J. 2014. An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species. In Vitro Cellular & Developmental Biology - Plant, 50, 110–120.
Li J, Wang X H, Wang X P, Wang Y J. 2015. Embryo rescue technique and its applications for seedless breeding in grape. Plant Cell Tissue and Organ Culture, 120, 861–880.
Liu Q, Zhang J, Wang Y, Yu D, Xia H. 2016. Breeding for cold-resistant, seedless grapes from Chinese wild Vitis amurensis using embryo rescue. New Zealand Journal of Crop and Horticultural Science, 44, 136–151.
Liu S M, Sykes S R, Clingeleffer P R. 2008. Effect of culture medium, genotype, and year of cross on embryo development and recovery from in vitro cultured ovules in breeding stenospermocarpic seedless grape varieties. Australian Journal of Agricultural Research, 59, 175–182.
Lloyd G, McCown B. 1980. Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Combined Proceedings, International Plant Propagators’ Society, 30, 421–427.
Moradi S, Daylami S D, Arab M, Vahdati K. 2017. Direct somatic embryogenesis in Epipactis veratrifolia, a temperate terrestrial orchid. Journal of Horticultural Science & Biotechnology, 92, 88–97.
Nitsch J, Pratt P C, Nitsch C, Shaulis N J. 1960. Natural growth substances in concord and concord seedless grapes in relation to berry development. American Journal of Botany. 47, 566–576.
Notsuka K, Tsuru T, Shiraishi M. 2001. Seedless-seedless grape hybridization via in-ovulo embryo culture. Journal of the Japanese Society for Horticultural Science, 70, 7–15.
Nuñez-Palenius H G, Klee H J, Cantliffe D J. 2006. Embryo-rescue culture of the ‘Galia’ muskmelon (Cucumis melo L. var. reticulatus Ser.) male parental line. Plant Cell Tissue Organ, 85, 345–352.
Pommer C V, Ramming D W, Emershad R L. 1995. Influence of grape genotype, ripening season, seed trace size, and culture date on in ovule embryo development and plant formation. Bragantia, 54, 237–249.
Ponce M T, Guiñazú M E, Tizio R. 2002. Improved in vitro embryo development of stenospermic grape by putrescine. Biocell, 26, 263–266.
Ramming D W. 1990. The use of embryo culture in fruit breeding. HortScience, 25, 393–398.
Ramming D W, Emershad R L. 1982. In ovulo embryo culture of seeded and seedless Vitis vinifera (Abstr.). HortScience, 17, 487.
Rehman M, Singh Z, Khurshid T. 2018. Pre-harvest spray application of prohexadione-calcium and paclobutrazol improves rind colour and regulates fruit quality in M7 Navel oranges. Scientia Horticulturae, 234, 87–94.
Royo C, Carbonell-Bejerano P, Torres-Pérez R, Nebish A, Martínez Ó, Rey M, Aroutiounian R, Ibáñez J, Martínez-Zapater J M. Developmental, transcriptome, and genetic alterations associated with parthenocarpy in the grapevine seedless somatic variant Corinto bianco. Journal of Experimental Botany, 67, 259–73.
Sánchez-Romero C, Perán-Quesada R, Márquez-Martín B, Barceló-Muñoz A, Pliego-Alfaro F. 2007. In vitro rescue of immature avocado (Persea americana Mill.) embryos. Scientia Horticulturae, 111, 365–370.
Seesangboon A, Gruneck L, Pokawattana T, Eungwanichayapant P D, Tovaranonte J, Popluechai S. 2018. Transcriptome analysis of Jatropha curcas L. flower buds responded to the paclobutrazol treatment. Plant Physiology and Biochemistry, 127, 276–286.
Sharma D R, Kaur R, Kumar K. 1996. Embryo rescue in plants: A review. Euphytica, 89, 325–337.
Singh N V, Singh S K, Singh A K. 2011. Standardization of embryo rescue technique and bio-hardening of grape hybrids (Vitis vinifera L.) using Arbuscular mycorrhizal fungi (AMF) under sub-tropical conditions. Vitis, 50, 115–118.
Singh Z, Brar S J S, Gosal S S. 1992. Ovule culture of seedless grapes Vitis vinifera L. cv. Perlette. Acta Horticulturea, 300, 325–330.
Spiegel-Roy P, Sahar N, Baron J, Lavi U. 1985. In vitro culture and plant formation from grape cultivars with abortive ovules and seeds. Journal of the American Society for Horticultural Science, 110, 109–112.
Stout A B. 1936. Seedlessness in grapes. New York State Agricultural Experiment Station, Technical Bulletin No. 238.
Tang D, Wang Y, Cai J, Zhao R. 2009. Effects of exogenous application of plant growth regulators on the development of ovule and subsequent embryo rescue of stenospermic grape (Vitis vinifera L.). Scientia Horticulturae, 120, 51–57.
Taşkın H, Yücel N K, Baktemur G, Çömlekçioğlu S, Büyükalaca S. 2013. Effects of different genotypes and gamma ray doses on haploidization with irradiated pollen technique in watermelon (Citrullus lanatus L.). Canadian Journal of Plant Science, 93, 1165–1168.
Uma S, Lakshmi S, Saraswathi M S, Akbar A, Mustaffa M M. 2011. Embryo rescue and plant regeneration in banana (Musa spp.). Plant Cell Tissue Organ, 105, 105–111.
Vaičiukynė M, ŽiaukaJ, Žūkienė R, Vertelkaitė L, Kuusienė S. 2018. Abscisic acid promotes root system development in birch tissue culture: A comparison to aspen culture and conventional rooting-related growth regulators. Physiologia Plantarum, 165, 114–122.
Wang Z W, Zhao M Z, Wu W M, Qian Y M, Yuan J. 2007. Study on techniques of ovule culture for seedless grape. Acta Agriculturae Jiangxi, 19, 27–29. (in Chinese)
Wei Z, Liu X H, Huang Y, Lu J, Zhang Y L. 2019. Volatile aroma compounds in wines from Chinese wild/hybrid species. Journal of Food Biochemistry, 43, e12684.
Xie K, Wang X, Wang H, Liang W, Xie Z, Guo D, Yi H, Deng X, Grosser J W, Guo W. 2014. High efficient and extensive production of triploid citrus plants by crossing polyembryonic diploids with tetraploids. Acta Horticulturae Sinica, 41, 613–620. (in Chinese)
Xie K D, Yuan D Y, Wang W, Xia Q M, Wu X M, Chen C W, Chen C L, Grosser J W, Guo W W. 2019. Citrus triploid recovery based on 2x × 4x crosses via an optimized embryo rescue approach. Scientia Horticulturae, 252, 104–109.
Xu H Y, Yan A L, Zhang G J. 2005. Determination of the proper sampling period for embryo rescue from crosses between diploid and tetraploid grape cultivars. Acta Horticulturae Sinica, 38, 629–633. (in Chinese)
Yan F F, Wang L H, Zheng X J, Luo Z, Wang J R, Liu M J. 2019. Acquisition of triploid germplasms by controlled hybridisation between diploid and tetraploid in Chinese jujube. The Journal of Horticultural Science and Biotechnology, 94, 123–129.
Yang D, Li W, Li S, Yang X, Wu J, Cao Z. 2007. In vitro embryo rescue culture of F1 progenies from crosses between diploid and tetraploid grape varieties. Plant Growth Regulation, 51, 63–71.
Zhu S P, Wu B, Ma Y Y, Chen J, Zhong G Y. 2013. Obtaining citrus hybrids by in vitro culture of embryos from mature seeds and early identification of hybrid seedlings by allele-specific PCR. Scientia Horticulturae, 161, 300–305.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
