园艺-分子生物合辑Horticulture — Genetics · Breeding
|PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit
|SONG Jun-xing, CHEN Ying-can, LU Zhao-hui, ZHAO Guang-ping, WANG Xiao-li, ZHAI Rui, WANG Zhi-gang, YANG Cheng-quan, XU Ling-fei
|College of Horticulture, Northwest A&F University, Yangling, P.R.China
Organic acids are one of the most important factors influencing fruit flavors. The predominant organic acid in most pear cultivars is malic acid, but the mechanism controlling its accumulation remains unclear. In this study, by comparing gene expression levels and organic acid content, we revealed that the expression of PbPH5, which encodes a P3A-ATPase, is highly correlated with malic acid accumulation in different pear species, with correlation coefficients of 0.932**, 0.656*, 0.900**, and 0.518* (*, P<0.05 or **, P<0.01) in Pyrus bretschneideri Rehd., P. communis Linn., P. pyrifolia Nakai., and P. ussuriensis Maxim., respectively. Moreover, the overexpression of PbPH5 in pear significantly increased the malic acid content. In contrast, silencing PbPH5 via RNA interference significantly decreased its transcript level and the pear fruit malic acid content. A subcellular localization analysis indicated that PbPH5 is located in the tonoplast. Additionally, a phylogenetic analysis proved that PbPH5 is a PH5 homolog gene that is clustered with Petunia hybrida, Malus domestica, and Citrus reticulata genes. Considered together, these findings suggest PbPH5 is a functionally conserved gene. Furthermore, the accumulation of malic acid in pear fruits is at least partly related to the changes in PbPH5 transcription levels.
Received: 03 March 2021
Accepted: 08 July 2021
This research was funded by the National Key Research and Development Program of China (2019YFD1001400) and the National Natural Science Foundation of China (31601715).
|About author: SONG Jun-xing, E-mail: JunxingSong@163.com; Correspondence YANG Cheng-quan, Tel: +86-29-87081023, E-mail: email@example.com; XU Ling-fei, Tel: +86-29-87081023, E-mail: firstname.lastname@example.org
Cite this article:
SONG Jun-xing, CHEN Ying-can, LU Zhao-hui, ZHAO Guang-ping, WANG Xiao-li, ZHAI Rui, WANG Zhi-gang, YANG Cheng-quan, XU Ling-fei.
PbPH5, an H+ P-ATPase on the tonoplast, is related to malic acid accumulation in pear fruit. Journal of Integrative Agriculture, 21(6): 1645-1657.
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