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Table of Content

    01 June 2021, Volume 54 Issue 11
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Genome-Wide Association Analysis of Superoxide Dismutase (SOD) Activity in Wheat Grain
    WANG JiQing,REN Yi,SHI XiaoLei,WANG LiLi,ZHANG XinZhong,SULITAN· GuZhaLiAYi,XIE Lei,GENG HongWei
    Scientia Agricultura Sinica. 2021, 54(11):  2249-2260.  doi:10.3864/j.issn.0578-1752.2021.11.001
    Abstract ( 565 )   HTML ( 55 )   PDF (2452KB) ( 739 )   Save
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    【Objective】The activity of superoxide dismutase (SOD) in wheat grains has a significant effect on the color and nutritional quality of wheat flour. Identification of associated loci and candidate genes for SOD activity in wheat grains is important for discovering the genetic mechanism of SOD activity in wheat grains and genetic improvement of wheat flour color. 【Method】The SOD activity of 212 common wheat varieties (lines) planted in 3 environments was detected by photoreduction method of nitro-blue tetrazolium (NBT), and the genome-wide association study (GWAS) of SOD activity in wheat grains was carried out by 16 705 high-quality SNP markers of 90K SNP chip, and candidate genes of significantly associated loci of stable inheritance were identified. 【Result】The phenotypic variation of SOD activity among wheat varieties (lines) was significant in different environments, with the coefficient of variation ranging from 4.34% to 5.23%, the correlation coefficient ranging from 0.60 to 0.90 (P<0.001). Polymorphic information content (PIC) ranging from 0.24 to 0.29 and the whole genome linkage disequilibrium (LD) attenuation distance of 7 Mb. The analysis of population structure showed that the tested materials could be divided into 3 subgroups. GWAS analysis showed that 29 loci (P≤0.001) were significantly associated with SOD activity, which were distributed on chromosomes 1A, 1B, 2A, 2B, 2D, 3B, 3D, 4B, 4D, 5A, 5B, 5D, 6A, 6B, 6D and 7B. A single locus can explain the phenotypic variation(R2) between 5.47% and 32.43%, of which 14 loci were detected in 2 or more environments. Nine significant associated loci were detected in three environments, distributed on chromosomes 1B, 2B, 4B, 5A, 5B, 6B and 6D, with a contribution rate of 6.21%-16.62%. SOD genes of TraesCS2B01G567600, TraesCS3D01G069900, TraesCS3D01G070200, TraesCS5B01G525700, TraesCS6A01G021400 and TraesCS6D01G431500, and SOD-activity-related candidate genes of TraesCS5A01G263500 and TraesCS6B01G707800 were used to identify the candidate genes of significantly associated loci of stable inheritance. The functions of the candidate genes were mainly related to the inhibition of cell reactive oxygen species accumulation and the participation in antioxidant regeneration. 【Conclusion】Twenty-nine SNP loci associated with SOD activity in wheat grains were detected, and 7 SOD genes and 2 candidate genes related to SOD activity were screened out.

    Genetic Research Advances on Maize Stalk Lodging Resistance
    WANG XiaQing,SONG Wei,ZHANG RuYang,CHEN YiNing,SUN Xuan,ZHAO JiuRan
    Scientia Agricultura Sinica. 2021, 54(11):  2261-2272.  doi:10.3864/j.issn.0578-1752.2021.11.002
    Abstract ( 755 )   HTML ( 88 )   PDF (2585KB) ( 669 )   Save
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    Maize stalk lodging has a great adverse effect on yield, quality and mechanized harvesting, and is one of the main problems to be solved urgently in current maize production and breeding. Strengthening the research on the lodging resistance of maize stalk will have great significance for improving the lodging resistance of maize. In this paper, we summarize the main factors affecting maize stalk lodging resistance, and their genetic mechanisms. The stalk lodging resistance is closely related to the stalk strength. The greater the stalk strength, the stronger the lodging resistance. The stalk strength is affected by the developmental stage, the internal and external structures of the stalk, and the components of the stalk cell wall. The meristem zone has vigorously dividing cells and is easily broken. After entering the reproductive growth, the rind and sclerenchyma tissue of the stalk are thickened, the vascular bundles are mature, and thus the stalk strength is enhanced. The main components of the stalk cell wall, including cellulose, hemicellulose, lignin, soluble sugars, inorganic substances, can improve the strength of the stalk. To date, based on the high-throughput phenotyping platforms, various maize linkage and natural populations, and mapping methods, researchers have identified a series of QTLs and candidate genes that affect stalk morphology, strength, and cell wall components. The studies have shown that the haplotype-based mapping method is better than SNP-based mapping method. Meta-QTL analysis integrates the mapping results of different genetic populations and can improve the versatility of QTLs. The genetic basis of stalk strength is complex, which is determined by polygenes with minor effect and additive effect. Candidate genes in the QTLs involve cell wall metabolism, transcription factors, protein kinases, and so on. MAIZEWALL is an important database of genes related to maize cell wall. So far, the database contains 1 156 candidate genes related to maize cell wall biology, which provides a powerful resource for research in this field. A series of genes affecting cell wall components, stalk morphology and stalk strength in maize have been identified. Their functions of these genes are related to cellulose synthesis pathways, such as genes of cellulose synthase, Cobra, glycosyltransferase and ribose transport; phenylpropane pathway genes, such as genes regulating bm1-bm5; plant hormones genes, such as genes related to gibberellin, auxin and brassinosteroid; transcription factors such as NAC, MYB; miRNA (ZmmiR528) and F-box genes (stiff1). In the future research, it is needed to explore the mechanical mechanism of stalk lodging at different developmental stages. Develop diverse natural populations and breeding materials for genetic analysis. Employ a various of mapping strategies to improve the efficiency of identification of the QTL and genes related to lodging resistance. Design various molecular markers based on the favorable alleles to improve the molecular marker assisted selection for lodging resistance. These efforts will promote the research of the genetic mechanism of stalk lodging resistance, and provide a reference for the molecular breeding of new varieties with strong lodging resistance.

    Characterizations of Transcriptional and Haplotypic Variations of SiTOC1 in Foxtail Millet
    ZHANG LinLin,ZHI Hui,TANG Sha,ZHANG RenLiang,ZHANG Wei,JIA GuanQing,DIAO XianMin
    Scientia Agricultura Sinica. 2021, 54(11):  2273-2286.  doi:10.3864/j.issn.0578-1752.2021.11.003
    Abstract ( 448 )   HTML ( 61 )   PDF (6448KB) ( 577 )   Save
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    【Objective】 Identification of allelic variations of heading date adaptation related genes and laying foundation for breeding of wide-adapted varieties in foxtail millet. 【Method】In this trial, a vital regulator of heading time in foxtail millet, SiTOC1, was identified using genome-wide association analysis. Spatio-temporal transcription (multi-omics database for Setaria italica,MDSi), sub-cellular localization and 24 hours rhythm expression pattern of SiTOC1 was analyzed. Sequence variations of both promoter and encoding regions in SiTOC1 and relationships between haplotypic variations and heading date were characterized in 99 foxtail millet accessions. 【Result】A significant GWAS signal (Position: 31 456 761 bp) was detected on Chromosome 1 and only one TOC1 homologue was identified (SiTOC1). SiTOC1 highly expressed in root, stem and leaf, and located into cell nucleus. An elevated expression of SiTOC1 was identified at dusk across whole day transcription survey under short-day environment. Many haplotypic variations of SiTOC1 were identified but REC and CCT domains of SiTOC1 were conserved in foxtail millet accessions, and two main haplotypes including H-2 and H-6 in protein encoding regions combined with two co-segregated haplotypes including Hp-591C and Hp-591A were identified. Nearly 2.5 times higher expression of Hp-591C haplotype combined with 9,11 and 12 days delay of heading time through Hainan, Changzhi and Urumuqi were observed. 【Conclusion】The major haplotype Hp-591A identified at 591 bp in the promoter region of SiTOC1 matures earlier than Hp-591C and could be selected as a main effective locus for molecular breeding of foxtail millet.

    TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION
    Physiological Mechanisms of Abiotic Stress Priming Induced the Crops Stress Tolerance: A Review
    WANG Xiao,CAI Jian,ZHOU Qin,DAI TingBo,JIANG Dong
    Scientia Agricultura Sinica. 2021, 54(11):  2287-2301.  doi:10.3864/j.issn.0578-1752.2021.11.004
    Abstract ( 1318 )   HTML ( 56 )   PDF (1536KB) ( 1227 )   Save
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    Abiotic stress factors, including heat stress, cold stress, drought stress, waterlogging stress etc., are the limiting factors for plant growth and crop production. Moreover, the frequency, extent and duration of abiotic stresses have predicted to be increased with global climate change. Therefore, improving crops resistance to abiotic stress or finding strategy to reduce the adverse effects of abiotic stress on crop yield and quality is of great significance for ensuring stable crop production and food security. “Priming” by exposing plants to moderate stress in the early growth stage can induce plant resistance to a later severe stress episode which happened during critical stage of the plant growth. The priming is an adaptive strategy that primed plants could effectively mount a faster and/or stronger defense response and actively improve the defensive capacity of plants under stress, compared with non-primed plants. According to the reoccurred stress types, the priming can be generally separated to four types, including in-generational stress tolerance (the priming stimulus and the stress occurred at later stage are the same stress type), intra-generational cross tolerance (the priming stimulus and the stress occurred at later stage are different stress types), transgenerational stress tolerance (the priming stimulus and the stress occurred at next generations are the same stress type), and transgenerational cross tolerance (the priming stimulus and the stress occurred at next generations are different stress types). In this review, the main physiological mechanisms were discussed, including plant photosynthetic response mechanism, antioxidant mechanism, and osmotic regulation, signal transduction mechanisms (plant hormones, Ca2+, hydrogen peroxide, nitric oxide, etc.), and epigenetic modification mechanisms (DNA methylation, histone modification, etc.). Then, the perspectives for the further research on understanding the underlying mechanisms of stress priming and the application of priming effects in the crop production were suggested. After revealing the mechanisms of priming, the candidate genes and proteins which play key regulatory roles in the acquisition of crop stress tolerance are found. And then, we can stimulate the related genes and protein expression which can actively induce the formation of stress tolerance in the critical crops growth stage, thereby effectively alleviating the adverse effects of abiotic stress on crop yield during the critical stage, which is meaningful for the crop production.

    Area Extraction and Growth Monitoring of Winter Wheat in Henan Province Supported by Google Earth Engine
    ZHOU Ke,LIU Le,ZHANG YanNa,MIAO Ru,YANG Yang
    Scientia Agricultura Sinica. 2021, 54(11):  2302-2318.  doi:10.3864/j.issn.0578-1752.2021.11.005
    Abstract ( 694 )   HTML ( 74 )   PDF (4856KB) ( 588 )   Save
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    【Objective】 The aim of this study was to use remote sensing technology to extract the spatial distribution information of winter wheat in Henan province from 2017 to 2020, and then to monitor the growth of winter wheat in 2020 with high frequency and to analyze the meteorological conditions. 【Method】 Based on the cloud platform of Google Earth engine (GEE), the selected Landsat 8 image data were synthesized according to the maximum value of NDVI, and then the features were constructed to add terrain features, texture features, NDVI and a new feature NDVI amplification. Random forest classification method was used to train the sample data according to the constructed features to extract the winter wheat planting area in Henan province from 2017 to 2020. The accuracy of the extracted winter wheat sown area was verified by confusion matrix and Henan statistical yearbook data. After accuracy verification, a mask was generated for the extracted winter wheat planting area in Henan province in 2020. In the mask area (winter wheat planting area) combined with MODIS high time resolution image data, the NDVI synchronization difference method was used to monitor the winter wheat growth from February to April in 2020. 【Result】 The GEE cloud platform could be used to quickly map the spatial distribution information of winter wheat planting areas in Henan province. Using random forest method to add terrain feature, texture feature, NDVI and new feature NDVI could effectively improve the extraction accuracy of winter wheat and reduce the relative error with statistical data. Based on confusion matrix, the average overall classification accuracy was 95.2%, the average kappa coefficient was 0.909, and the average classification accuracy of winter wheat was 95.3%. Compared with the statistical yearbook data of Henan province, the relative errors of winter wheat sown area extracted by this method in Henan province from 2017 to 2019 were all less than 3%. The average relative error of winter wheat sown area in the main planting areas of winter wheat in Henan province was less than 6%. MODIS image data combined with NDVI difference model could be used to monitor the growth of winter wheat in Henan province in 2020. The growth of winter wheat in Henan province was better than that of previous years and 2019 during the return to green period. In the later growth stage of winter wheat, the growth of most areas was the same as that of previous years and 2019. On the whole, the growth of winter wheat in 2020 was better than that of previous years and 2019. 【Conclusion】 The method proposed in this paper could carry out high-precision extraction and high-frequency growth monitoring of winter wheat in Henan province, and could provide a scientific basis for local governments or some agricultural departments in arranging and guiding agricultural activities.

    Effects of Density and Row Spacing on Seedling Traits of Rapeseed and Seed Yield
    KUAI Jie,LI Zhen,WANG Bo,LIU Fang,YE Jun,ZHOU GuangSheng
    Scientia Agricultura Sinica. 2021, 54(11):  2319-2332.  doi:10.3864/j.issn.0578-1752.2021.11.006
    Abstract ( 403 )   HTML ( 31 )   PDF (1064KB) ( 591 )   Save
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    【Objective】The relationship between seedling growth and yield formation of Brassica napus L. under different densities and row spacings was studied, which laid a theoretical foundation for further increasing yield and reducing yield gap, and it also helped to understand the mechanism of yield regulation in rapeseed with high-density planting. 【Method】The genotype HZ 62 was selected as the test material in 2016-2017, and HZ 62 and variety 1301, with different plant architecture, were grown in 2017-2018. The planting density was set as main plots at 15×104plants/hm2 (D1), 30×104 plants/hm2 (D2) and 45×104 plants/hm2 (D3), with the row spacing as subplots of 15 cm (R15), 25 cm (R25) and 35 cm (R35). The changes of dry matter accumulation and distribution in different organs, carbon and nitrogen metabolism in stem and leaf, root activity and yield at maturity were studied. 【Result】 The results showed that: the root diameter, root dry weight, shoot dry weight and plant height decreased at maturity, and the effective branch number decreased with increased plant density, and the decrease rate became smaller with narrowing row spacing at the same density. Under the density of D1, D2 and D3, the above indices had the best performance at R25, R15 and R15. Compared with D1R25, the yield of HZ62 and 1301 decreased by 57.14% and 55.73% respectively, but the population yield increased by 21.55% and 30.92% in 2017-2018 under D3R15. The correlation analysis showed that there was a highly significant positive correlation between leaf dry matter allocation rate and individual plant yield, also, between stem and root dry matter allocation rate and population yield. Further analysis of the growth indexes showed that with the increase of plant density, leaf SPAD value, root biomass per plant, root bleeding sap and root activity decreased significantly, while the root biomass increased significantly. Under the same density, the leaf SPAD value, leaf and stem C/N ratio, leaf LAI and root biomass of the population increased by adjusting row spacing and decreasing the difference between plant spacing and row spacing, which laid a foundation for the yield at maturity. In 2017-2018, compared with D1R25, the C/N ratio of the stem decreased by 22.95%, the root biomass, bleeding snap and root activity of individual plant decreased by 35.60%, 16.07% and 15.51% respectively, while leaf C/N ratio and root biomass in population increased by 16.11% and 83.44% respectively for HZ62; the stem C/N ratio decreased by 19.71%, the root biomass per plant, bleeding snap and root activity decreased by 30.87%, 22.63% and 22.85% respectively, while leaf C/N ratio and root biomass in population increased by 14.84% and 108.21% respectively for 1301 under D3R15. 【Conclusion】Under the condition of this experiment, compared with the traditional arrangement of plant density and row spacing, the increasing plant density and narrowed row spacing could promote the nitrogen metabolism of individual leaf and increase the leaf SPAD value, the photosynthetic leaf area and the biomass as well as activity of root at the seedling stage to achieve higher yield.

    PLANT PROTECTION
    Epidemic Factors Affecting the Infection and Occurrence of Valsa mali
    LIN Xiao,SUN ChuanRu,WANG CaiXia,LIAN Sen,DONG XiangLi,LI BaoHua
    Scientia Agricultura Sinica. 2021, 54(11):  2333-2342.  doi:10.3864/j.issn.0578-1752.2021.11.007
    Abstract ( 385 )   HTML ( 28 )   PDF (2873KB) ( 256 )   Save
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    【Objective】Valsa canker, mainly causing dead branches and dead trees, is an important branch disease of apple trees. The objective of this study is to clarify the impact of environmental factors such as low temperature and host factors such as the age of branches on the infection and occurrence of valsa canker, to analyze the causes of the disease epidemics, and to provide a basis for the prediction and control of the disease. 【Method】Inoculation experiments were conducted under artificial controlled environmental conditions. The conidia colonized ratios of Valsa mali on different parts of apple branches were detected, the colonization sites of the pathogen in wounds were examined, and effects of the factors such as freezing, ice forming on branches, loss of water of branches, and age of branches on the infection of the pathogen and occurrence of the disease were tested in the experiments. 【Result】The colonized percentages of V. mali, i.e. the site carried the pathogen, on the 7 different branch sites of the apple trees inoculated with conidial suspension in the August were close to or more than 90% when examined in March of the following year. The pathogen inoculated on the wounds mainly colonized, grew and expanded in the necrotic tissue of the wounds, but did not penetrate the cork layer around the wound and invade the living cortical tissue to cause canker disease. Among the 6 examined branch sites, fresh wounds were the most susceptible to infection of V. mali, with the highest disease incidence of inoculated sites, followed by fruit stalk scars. The forks, bud eyes and fruit moss were less susceptible and disease incidences of inoculated sides were slightly lower. The lenticels were resistant to pathogen infection and no inoculated sites developed to canker. Both freezing and freezing after soaking (form ice on branch surface) can increase the susceptibility of branches to infection of V. mail. In the tested branches, the disease incidence of inoculated sites on the branches treated at -25 and -18℃ were significantly higher than that treated at -10, -7 and 0℃, and the incidence on branches frozen after soaking (simulating branch freezing after precipitation in winter) was significantly higher than that of uniced branches treated at the same temperature. Among the branches that were frozen and frozen after soaking, the disease incidence of inoculated sites on 1-year-old branches was significantly higher than that of 2-year-old branches. The disease incidence of inoculated sites at the tips of 1-year-old branches was significantly higher than that at the base of the same branches when frozen after soaking. The branches subjected to water loss after frozen (simulating the branches that lose water due to wind and high temperature after overwintering) were more susceptible to the infected V. mali, and the disease incidence of inoculated sites was significantly higher than that on frozen branches after soaking. The greater the amount of water losing, the higher the disease incidence, and the highest disease incidence of inoculated bud eye can reach 85%. 【Conclusion】V. mali is easy to colonize on apple branches, and colonized pathogens mainly survive and grow in dead tissues in wounds or on the surface of branches. Whether colonized pathogens can infect and cause disease mainly depends on environmental factors destroying the cork layer of the branches. Freezing, especially freezing damage at the temperature below -15℃ can destroy the cortex and cork layer of the branches, and increase the susceptibility of apple branches to infection of V. mali. Compared with freezing, freezing that form ice crystals on the surface of branches lead to a greater damage to the cork layer of branches, and the branches are more susceptible to the infection of the pathogen. The damage of water loss after freezing to the branches is particularly serious. The loss of water after the branches overwinter can significantly increase susceptibility of the branches to infection of the pathogen. The age of the branches is different, the developmental maturity, strength and toughness of the cork layer are different, and the degree of damage to the cork layer is also different when the branches were affected by adverse environmental factors. The structure of cortex in different sites of the tree is different, and there are obvious differences in the susceptibility to the pathogen infection.

    Effect of Trap Color and Position on the Trapping Efficacy of Tuta absoluta
    ZHANG GuiFen,ZHANG YiBo,LIU WanXue,ZHANG Fan,XIAN XiaoQing,WAN FangHao,FENG XiaoDong,ZHAO JingNa,LIU Hui,LIU WanCai,ZHANG XiaoMing,LI QingHong,WANG ShuMing
    Scientia Agricultura Sinica. 2021, 54(11):  2343-2354.  doi:10.3864/j.issn.0578-1752.2021.11.008
    Abstract ( 403 )   HTML ( 31 )   PDF (1181KB) ( 322 )   Save
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    【Background】The tomato leafminer Tuta absoluta, originates in Peru, the western South America, is a newly invaded important agricultural pest in China and has posed a potential threat to safety production of agriculture in China. 【Objective】To provide a basis for effectively managing the occurrence and damage caused by this insect pest, the trapping efficacy of color and height of placement of sex pheromone traps (planar traps) on T. absoluta was evaluated. 【Method】The sex pheromone lures target to T. absoluta were used and the techniques and methods of behavioral ecology were applied. The field tests were conducted in greenhouses for organic vegetable production. 【Result】The four colors of sex pheromone traps were effective in trapping T. absoluta. On average, the total numbers of trapped adults per trap were 2 548.3 males by blue (wavelength, (465±10) nm) trap, 2 065.8 males by yellow ((575±10) nm) trap, 1 998.3 males by green ((520±10) nm) trap, and 1 072.7 males by white trap during the whole period (61 d) of field trapping tests. The daily proportions of trapped adults by the blue and yellow traps were the highest, and then followed by the green trap, that of the white trap was the lowest. The daily number of trapped T. absoluta adults by the blue trap was the most, and then followed by the yellow and green traps, that of the white trap was the least. The trapping stability of white trap was significantly higher than that of the other three trap colors. Comprehensive evaluation, based on above three indexes, showed that the trapping efficacy of the blue trap to T. absoluta adults was the highest, and then followed by that of the green and yellow traps, that of the white trap was the lowest. The numbers of trapped adults per sex pheromone trap were 332.2 males by the height of 0 cm (place the traps on the ground directly), 94.2 males by 0-20 cm, 12.4 males by 60-80 cm, 9.2 males by 120-140, and 7.4 males by 180-200 cm on average during the whole period (37 d) of field trapping tests. The proportion of trapped adults by the trap height of 0 cm was the highest, and then followed by the 0-20 cm, those of the 60-80, 120-140, and 180-200 cm were the lowest. The daily number of trapped T. absoluta adults by the trap height 0 cm was the most, and then followed by the 0-20 cm, those of the 60-80, 120-140, and 180-200 cm were the least. Significant difference was detected among the five trap heights when the trapping stability was compared. Comprehensive evaluation, based on above three indexes, showed that the trapping efficacy of the trap height 0 cm to T. absoluta adults was the highest, and then followed by that of the 0-20 cm, the next was the 60-80 cm, those of the 120-140 cm and 180-200 cm were the lowest. 【Conclusion】Analyses based on the integrated indexes of color and height of placement of sex pheromone traps (planar traps) showed that blue traps placed on the ground directly could get the best trapping efficacy.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    A Meta-Analysis of Effects of Nitrogen Management on Winter Wheat Yield and Quality
    DENG LiJuan,JIAO XiaoQiang
    Scientia Agricultura Sinica. 2021, 54(11):  2355-2365.  doi:10.3864/j.issn.0578-1752.2021.11.009
    Abstract ( 421 )   HTML ( 39 )   PDF (2990KB) ( 454 )   Save
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    【Objective】Nitrogen (N) is one of the key factors affecting wheat yield and grain protein concentration. However, the continuous improvement of wheat yield diluted the protein concentration of the grain to some extent, so it was difficult to achieve high yield and high quality in wheat. How to achieve synergistic improvement of wheat yield and grain quality through optimized N management is the key to sustainable wheat production. 【Method】This study collected 2 758 research cases on wheat yield and protein quality published between 1990 and 2017, and the effects of N management on winter wheat yield and quality were evaluated by using meta-analysis and combined with N flow analysis methods. Combined with N flow analysis method, a comprehensive N management measure is proposed to improve wheat quality and efficiency. 【Result】 From 1990 to 2017, the wheat yield increased by 42%±1.2% and the grain protein concentration increased by 19%±0.7% by applying N fertilizer. With the increase of N application rate, both wheat yield and grain protein concentration showed an increasing and then decreasing trend. The effect of N application on increasing yield was most significant when the N application rate was 200-250 kg·hm-2, and the effect of increasing grain protein concentration was most significant when the N application rate was 384 kg·hm-2. The effects of increasing wheat yield and increasing grain protein concentration were most significant when the dressing ratio was 1-2. In comparison with the N application rate >300 kg·hm-2, controlling the N application rate at 200-250 kg·hm-2 could effectively reduce N loss and improve N use efficiency. Under the same conditions as other management measures, compared with a single optimized N application rate or N application dressing ratio, a comprehensive optimization of N application rate and basal-dressing ratio could increase the yield-increasing effect of N application by 8%-30%, and the quality-improving effect could increase by 19%-21%. 【Conclusion】 Increasing N application can realize the wheat yield and grain protein concentration increase, different N application rate and the dressing ratio have a significant effect on yield and quality improvement. The integrated N management measures of comprehensive optimization of N application rate and dressing ratio can not only synergistically achieve the goals of high yield and good quality of wheat, but also reduce environmental emissions, which provides case support for sustainable wheat production management in the future.

    Effects of Tillage with Mulching on Soil Aggregate Structure and Water Use Efficiency of Potato in Dry-Farming Area of Southern Ningxia
    MIAO FangFang,MIAN YouMing,PU XueKe,WU ChunHua,ZHOU YongJin,HOU XianQing
    Scientia Agricultura Sinica. 2021, 54(11):  2366-2376.  doi:10.3864/j.issn.0578-1752.2021.11.010
    Abstract ( 305 )   HTML ( 26 )   PDF (1040KB) ( 305 )   Save
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    【Objective】The purpose of this study was to investigate the effects of tillage with mulching on soil aggregate structure and water use efficiency of potato in southern Ningxia where is a typical semiarid rain-fed area. 【Method】A four years term field experiment of three growing season was carried out from 2013 to 2016, and three tillage methods (subsoiling, no tillage and ploughing) with three mulching measures (straw mulching, film mulching and no mulching) were set up to study the effects of different tillage methods combined with mulching measures on soil aggregate quantity, soil water storage capacity, potato yield and water use efficiency. 【Result】 Tillage methods, mulching measures and their interaction could significantly increase the >0.25 mm aggregate size fraction in 0-40 cm layer. In 0-20 cm layer, compared with ploughing tillage without mulching treatment, subsoiling with straw mulching treatment significantly increased the >0.25 mm aggregate size fraction by 14.2% and 16.9% in dry year (2016) and deficit year (2014), respectively, while no tillage with straw mulching treatment was significantly increased by 8.5% in the normal year (2015). In 20-40 cm soil layer, subsoiling with straw mulch in dry year, subsoiling with film mulch in deficit year, and no tillage with straw mulch in normal year had the best effects in the >0.25 mm aggregate size fraction, which were significantly increased by 18.2%, 21.5% and 18.7%, respectively, compared with ploughing tillage without mulching. The tillage methods, mulching measures, and their interactions had significant effects on the soil water storage (0-200 cm). Compared with ploughing without mulching treatment, subsoiling with straw mulching treatment significantly increased the mean soil water storage in fallow period by 29.6%, 9.3%, and 11.4%, respectively. Compared with ploughing tillage without mulching, subsoiling with straw mulching treatment in dry year and deficit year significantly increased the mean soil water storage in key growth period by 21.9% and 28.3%, respectively, while no tillage with straw mulching treatment in normal year significantly increased the mean soil water storage in key growth period by 17.1%. In deficit year, the mulching methods and the interaction of tillage with mulching had significant effects on the yield and water use efficiency of potato, while no significances were found among the tillage methods. Compared with the ploughing without mulching, no tillage with straw mulch significantly increased the potato yield and water use efficiency by 51.8% and 50.5%, respectively. In normal year and dry year, the tillage methods, mulching measures and their interactions had highly significant effects on the potato yield and water use efficiency, and treatment of subsoiling with straw mulch had the best effect. The yield and water use efficiency of subsoiling with straw mulching treatment was 56.19% and 44.84% higher than that of ploughing tillage without mulching, respectively. 【Conclusion】Tillage combined with mulching could improve soil aggregate structure, significantly enhance soil water storage and moisture conservation capacity in fallow and growth periods, and significantly improve potato yield and water use efficiency. Subsoiling combined with straw mulch in normal and dry years, and no tillage combined with straw mulch in deficit years could achieve continuous yield increase of potato in southern Ningxia.

    Analysis of Yield Gaps and Limiting Factors in China’s Main Sugarcane Production Areas
    ZHOU YiFan,YANG LinSheng,MENG Bo,ZHAN Jian,DENG Yan
    Scientia Agricultura Sinica. 2021, 54(11):  2377-2388.  doi:10.3864/j.issn.0578-1752.2021.11.011
    Abstract ( 627 )   HTML ( 28 )   PDF (3584KB) ( 319 )   Save
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    【Objective】Although China is the third biggest country in sugarcane planting areas and total production in the world, the sugarcane yield per hectare in China is much lower than the other high-yield sugarcane producing countries. This paper studied sugarcane yield potentials and current yield gaps in different sugarcane producing areas in China, and then the potential to reduce yield gap by optimizing yield-limiting factors was discussed, and thus to provide reference for increasing yield and efficiency in sugarcane production. 【Method】This study was based on data collection including statistical data and experiment data from literatures. A total of 147 data sets of sugarcane yield and planting areas from 1999 to 2018 in different provinces (autonomous regions, cities) were obtained from the national statistics database. Based on the database of CNKI and Web of Science, experiment data about planting density, fertilization rate, variety and yield from 93 papers were collected for main sugarcane producing areas, including 54 papers in Guangxi, 14 papers in Yunnan, and 25 papers in Guangdong. In each producing area, the mean of upper 5% experimental yields was used as yield potential, and the mean of statistical data as farmer’s yield, and yield gap was calculated as the difference between yield potential and farmer’s yield. Then the relationships between yield and fertilization rate, planting density and variety were analyzed, and therefore recommendations of optimized fertilization rate, planting density, variety and the potential to reduce yield gap were obtained. 【Result】Guangxi, Yunnan and Guangdong were the three main sugarcane producing areas in China. The sugarcane yield potentials were 137.1 t·hm-2in Guangxi, 147.2 t·hm-2in Yunnan, and 145.8 t·hm-2in Guangdong, respectively; while farmer’s yield were 74.2, 62.0, 78.3 t·hm-2, respectively, only achieving 54.1%, 42.1%, and 53.7% yield potential for each area, respectively. Fertilization rate, planting density and variety had significant effects on sugarcane yield, and there was big potential to optimize these yield-limiting factors. The fertilization recommendations were 270 kg N·hm-2, 99 kg P2O5·hm-2, 208 kg K2O·hm-2 in Guangxi, 228 kg N·hm-2, 117 kg P2O5·hm-2, 281 kg K2O·hm-2 in Yunnan, and 240 kg N·hm-2, 71 kg P2O5·hm-2, 193 kg K2O·hm-2 in Guangdong. The recommended planting density in Guangxi, Yunnan, Guangdong was 8×104-10×104, 10×104-12×104, 8×104-10×104 buds/hm2, respectively, and the varieties with high yields were GF series, GT series and YT series, respectively. 【Conclusion】According to the results, the potentials of yield increase in Guangxi, Yunnan, Guangdong were 62.9, 85.2, and 67.5 t·hm-2, respectively. Fertilization optimization could increase yield 16.9, 28.4, and 25.3 t·hm-2 for each area. Optimizing planting density could increase yield 23.6, 27.9, 22.1 t·hm-2, and optimizing sugarcane variety could increase yield 26.8, 42.4, and 15.1 t·hm-2, respectively.

    HORTICULTURE
    A New Classification Standard for Different Grape Cluster Shapes and Investigation on Cluster Shape Dynamic Development Process
    HUANG YuQing,SUN YanYan,LUO RongZheng,Awangcuomu ,LU SuWen,FAN XiuCai,WANG Chen,LIU ChongHuai,FANG JingGui
    Scientia Agricultura Sinica. 2021, 54(11):  2389-2405.  doi:10.3864/j.issn.0578-1752.2021.11.012
    Abstract ( 428 )   HTML ( 32 )   PDF (7781KB) ( 420 )   Save
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    【Objective】Grape is an important economic fruit tree, whose economic value was affected by the cluster shape. The study investigated the cluster development of 255 grape germplasm resources to establish a new classification standard including the detailed classification method of grape inflorescence and cluster shape, in order to provide new theoretical support for grape production. 【Method】The cluster length and width variation of 255 grape germplasm resources were tracked in the field. From three key growth periods (flowering, fruit expansion and fruit maturity), cluster length and width, six spikelet length and width, spikelet angle, fruit grain diameter, fruit grain weight and carpopodium length were measured to analyze the variation modes during dynamic development process of cluster shape. 【Result】According to the investigation of 255 grape germplasm resources, a new classification standard was established to divide the shape into long conical, short conical, long cylindrical and short cylindrical. It was found that 52.16% of inflorescence would change shape during the development process, and most of them was the long cylindrical inflorescence which could develop into short cylindrical cluster. Therefore, there were 16 cluster shape change types. The analysis of cluster length and width showed that the long conical cluster had high “stability” during the development process, and the length had more influence on shape for short conical, long cylindrical and short cylindrical clusters. Further investigation represented that the length, width and angle of spikelet, especially the top spikelet were the main factors affecting the outer edge of cluster shape. 【Conclusion】The study divided inflorescence and cluster shapes into four types: long cone, short cone, long cylinder and short cylinder, and a based classification standard was established. It was found through investigation that more than half of grape inflorescences changed in shape, of which the conical type was relatively stable. Cluster length and width, spikelet length, width and angle had more influence on shape change, which were reflected in the indicators and outer edge, especially the figures of top spikelet angle were the main affection to judge type. Spikelet angle variations reflected on the effective length in the shape. This study further proposed pruning method based on the shape of inflorescences and clusters, in order to provide theoretical basis for grape management.

    Diversity Analysis of Phenotypic Characters in Germplasm Resources of Ornamental Peaches
    ZHANG BinBin,CAI ZhiXiang,SHEN ZhiJun,YAN Juan,MA RuiJuan,YU MingLiang
    Scientia Agricultura Sinica. 2021, 54(11):  2406-2418.  doi:10.3864/j.issn.0578-1752.2021.11.013
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    【Objective】 The aim of this study was to investigate the feasibility of a phenotypic evaluation of the qualitative and quantitative characters present in the germplasm resources of ornamental peaches, and these findings could provide a scientific basis for the evaluation and selection of new varieties of ornamental peaches. 【Method】 Ten qualitative characters (flower type, petal type, petal color, height of pistil surface, pollen fertility, inter calyx tub color, anther color, leaf color, tree habit, and tree vigor) and six quantitative characters (flower size, internode length, flower bud/leaf bud, simple bud/multiple bud, site of first node with flower bud, and growth period) of 38 germplasm resources of ornamental peaches were investigated. The qualitative characters were graded by assignment method. After correlation analysis, the principal component analysis and cluster analysis of the phenotypic data were conducted. 【Result】 The diversity and degree of variation of quantitative characters in the germplasm resources were higher than that of the qualitative characters. The correlation analysis indicated that there was a relationship among some of the different characters, so that the information of the characters was overlapped. The principal component analysis showed that 16 characters were transformed into 10 principal components, and the first six principal components reflected the main phenotypic characters of 38 germplasm resources of ornamental peaches. The phenotypic factors contained in these principal components could be used as the main character indexes for germplasm innovation and parent selection for breeding ornamental peaches. The original data of 16 characters of 38 germplasm resources of ornamental peaches were standardized, and the cluster analysis was conducted using Euclidean distance and the sum of squares method. The germplasm resources were clustered into eight groups with a genetic distance of nine. The most obvious characteristics, such as petal type, leaf color, tree habit, and tree vigor, were the main phenotypic characters that could be used for distinguishing different groups. 【Conclusion】 This qualitative and quantitative study showed that it was feasible to analyze and evaluate the diversity of germplasm resources of ornamental peaches based on their phenotypic characters.

    FOOD SCIENCE AND ENGINEERING
    Research Progress of Food-Borne Pathogen Detection Based on Electrochemical and Optical Aptasensors
    HUI YuanYuan,PENG HaiShuai,WANG BiNi,ZHANG FuXin,LIU YuFang,JIA Rong,REN Rong
    Scientia Agricultura Sinica. 2021, 54(11):  2419-2433.  doi:10.3864/j.issn.0578-1752.2021.11.014
    Abstract ( 405 )   HTML ( 36 )   PDF (1148KB) ( 631 )   Save
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    Food safety has become a heated topic attracting widespread attention from the society due to the diversity of food and complexity of food production system. Food-borne diseases caused by microorganisms have the highest rates in food safety problem. The detection of food-borne pathogenic bacteria is the key link for the food-borne disease prevention and control. The plate counting method is rated as the gold standard for microbial detection, but the signal amplification was achieved by the growth of individual bacterial cells into visible colony during the detection of pathogenic bacteria, so the detection time is longer (3-7 days). Although polymerase chain reaction (PCR) and enzyme-linked immunoabsorbent assay (ELISA) are now applied in the detection of food-borne pathogenic bacteria, they are not suitable for timely and rapid on-site detection due to time-consuming pretreatment, complex operations and false positive results. Aptamers (Apt) are oligonucleotides that are isolated from combinatorial DNA library via systemic evolution of ligands by exponential enrichment (SELEX) technology, which present great potential in the field of detection of toxin, heavy metal, antibiotics, food pathogens and other small molecules due to their small size, easy synthesis and modification. Currently, many researchers of domestic and overseas have developed various detection methods by using aptamer as bio-recognition elements. This paper reviewed common food-borne pathogenic bacteria, traditional detection methods of food-borne pathogenic bacteria, and the electrochemical and optical aptasensors for detection of food-borne pathogenic bacteria in recent years. The descriptions covered the detection strategy of each method and provided details such as the detection time, range and limit. Finally, the paper pointed out the flaw of aptasensor in food safety detection, and the research development tendency was prospected, which provided bases for the further related work.

    ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT
    Transient Expression and the Effect on Proliferation and Apoptosis of Granule Cell Stimulating Factor in Ovarian Fibroblasts
    LI RunTing,CHEN LongXin,ZHANG LiMeng,HE HaiYing,WANG Yong,YANG RuoChen,DUAN ChunHui,LIU YueQin,WANG YuQin,ZHANG YingJie
    Scientia Agricultura Sinica. 2021, 54(11):  2434-2444.  doi:10.3864/j.issn.0578-1752.2021.11.015
    Abstract ( 307 )   HTML ( 23 )   PDF (1756KB) ( 226 )   Save
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    【Objective】 The purpose of this paper is to study the transient expression of granule cell stimulating factor (GCSF) in ovarian fibroblast cells, and the influence of GCSF on proliferation, cell cycle, and apoptosis, to provide theoretical basis for molecular genetic breeding of sheep pluripotent stem cells induced by GCSF in the future. 【Method】 The sheep GCSF eukaryotic expression plasmid pRTL1-GCSF and the control vector plasmid pRTL1 were transfected into 1×105 cells·mL-1 sheep fibroblasts respectively. After 48 h of culture, the total RNA was extracted by Trizol method and reverse transcribed into cDNA. The transient expression level of sheep GCSF in fibroblasts was detected by real-time quantitative PCR. GCSF dependent cell line NFS-60 was used for the biological activity of GCSF secreted and expressed in the supernatant of sheep fibroblasts 48 hours after transfection, which was determined by cell viability detection reagent alamarBlue. The HEK 293F suspension culture was used to express the secreted GCSF protein. The GCSF protein expressed in the cell culture medium was purified by Ni-NTA resin and detected by SDS-PAGE. After adding the 30 ng·mL-1 purified GCSF protein, the proliferation of sheep fibroblasts was detected by alamarBlue at 24 h and 48 h, and the cell cycle and apoptosis of sheep fibroblasts were detected by flow cytometry. 【Result】 The expression level of GCSF in sheep fibroblasts was significantly increased after transfection for 48 h. In sheep fibroblasts, the expression level of GCSF transfected with pRTL1-GCSF plasmid was 50 615.92 ± 4 738.83 of that of pRTL1 empty control group. The fluorescence intensity of NFS-60 in the experimental group and positive control group was significantly higher than that in the negative control group and blank control group (P<0.01), but there was no significant difference between the experimental group and the positive control group (P>0.05). The results showed that sheep GCSF could significantly stimulate the proliferation of NFS-60 cells, indicating that the GCSF expressed in sheep fibroblasts had biological activity. After eukaryotic expression of secretory GCSF protein in HEK 293F cell line, the sheep GCSF protein was purified. After 30 ng·mL-1 sheep GCSF was added to sheep fibroblasts, the cell viability of GCSF test group was not significantly different from that of culture medium dilution control group for 24 h and 48 h, but the distribution of cell cycle was significantly changed. At 24 h, compared with the control group, the proportion of G1 phase cells increased from (55.29±1.68)% to (69.37±0.24)%, the difference was very significant (P<0.01); the proportion of S phase cells changed from (15.99±0.38)% to (15.39±0.60)%, the difference was not significant (P>0.05); G2/M phase cells increased significantly (P<0.05), and the proportion increased from (22.88±1.00)% to (26.76±0.82)%. The results showed that 24 hours after the addition of sheep GCSF, the number of cells in division and interphase increased significantly. At 48 h, compared with the control group, the proportion of G1 phase cells decreased from (65.96±0.37)% to (45.69±0.26)%, the difference was very significant (P<0.01); the proportion of S phase cells increased from (13.45±1.33)% to (37.87±2.43)%, the difference was very significant (P<0.01); the proportion of G2/M phase cells changed from (16.42±1.29)% to (21.80±1.86)%, the difference was not significant (P>0.05). The results showed that the number of cells in interphase was significantly decreased and the number of cells in DNA replication state increased significantly at 48 h after adding GCSF. Compared with the control group, the apoptosis rates of the control group (Ctr) and the experimental group (GCSF) were (7.51±0.38)% and (9.16±0.46)% respectively at 24 h culture. At 48 h, the apoptosis rates of the control group and the experimental group were (5.73±0.29)% and (5.39±0.27)%, respectively. At 72 h, the apoptosis rates of control group (Ctr) and experimental group (GCSF) were (8.88±0.45)% and (5.41±0.27)%, respectively. There was a significant difference between 24 h and 72 h (P<0.01), but there was no significant difference at 48 h (P>0.05). The results showed that GCSF promoted the apoptosis within 24 hours, and the apoptosis was inhibited with the prolongation of time. 【Conclusion】 In conclusion, sheep fibroblasts can express GCSF instantaneously and have biological activity. GCSF did not affect the proliferation of sheep fibroblasts, but could regulate its cell cycle and affect cell apoptosis. The results laid a foundation for breeding sheep with high immunity and disease resistance by GCSF mediated by sheep fibroblasts.

    Research Progress of Intelligent Sensing Technology for Diagnosis of Livestock and Poultry Diseases
    LI QiFeng,LI JiaWei,MA WeiHong,GAO RongHua,YU LiGen,DING LuYu,YU QinYang
    Scientia Agricultura Sinica. 2021, 54(11):  2445-2463.  doi:10.3864/j.issn.0578-1752.2021.11.016
    Abstract ( 803 )   HTML ( 73 )   PDF (1104KB) ( 965 )   Save
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    Animal husbandry is an important part of agriculture. At present, animal husbandry is developing towards large-scale and intensive development, which also increases the difficulty of diagnosis of livestock and poultry diseases. In recent years, in order to improve the level of animal welfare in livestock and poultry breeding, and to reduce the economic losses and public health safety risks caused by animal diseases and health abnormalities in livestock breeding, a number of automated methods for the diagnosis and treatment of livestock and poultry diseases through digital and intelligent means have emerged, such as machine vision analysis, animal audio analysis, infrared temperature perception, deep learning classification, etc. These methods could effectively improve the diagnosis efficiency of diseased or abnormal livestock and poultry, shorten the diagnosis cycle, and reduce the labor force of manual inspection in animal husbandry. The automatic diagnosis and treatment method of livestock and poultry diseases is different from the conventional diagnosis methods based on pathological knowledge, which mainly uses various sensors to automatically obtain various characteristics information of livestock and poultry during the breeding process, such as images, sounds, body temperature, heart rate, and excrement. The collected information is comprehensively analyzed and processed through mathematical models, such as Mel cepstrum coefficient, Logistics regression analysis and intelligent algorithms such as support vector machines and deep learning, and then the animal’s health status is evaluated and predicted. The current research progress of animal disease intelligent diagnosis technology and some basic method principles was summarized from several aspects, such as livestock and poultry morphological diagnosis technology, behavior diagnosis technology, sound diagnosis technology, body temperature diagnosis technology, and other physiological parameter diagnosis technology. Those methods were based on the digital characteristics of animal appearance and body size, behavior and movement, call and sound, body temperature, excrement, respiration and heart rate, the characteristics collected by the sensor, which were analyzed and classified in real time through mathematical models, and the analysis was basically achieved. The current research results on automatic diagnosis and treatment of livestock and poultry diseases were abundant, but most of the related diagnosis methods were carried out in an ideal environment. However, the interference factors in the actual production and breeding environment were very large, and the most of the current diagnostic methods could not eliminate the interference well and accurately extract the required characteristic information. Besides, the current digital livestock disease diagnosis methods were mostly based on the analysis and diagnosis of one kind of livestock feature information, which affected the diagnosis accuracy of the diagnosis system and the diagnosis results were not convincing. At the same time, the most of the current digital diagnosis methods for poultry and livestock diseases still had some problems such as poor diagnosis generalization ability and poor anti-interference ability, which restricted their promotion and application. The focus of future research on automatic diagnosis of livestock and poultry diseases is to improve the accuracy of its sensing algorithms and the applicability and robustness of mathematical models, and to develop an intelligent diagnosis and treatment expert system for livestock and poultry diseases based on multiple feature coupling and data fusion, realize real-time, efficient, intelligent and accurate livestock and poultry health diagnosis.

    The Capping Pheromone Contents and Putative Biosynthetic Pathways in Larvae of Honeybees Apis cernana
    QIN QiuHong,HE XuJiang,JIANG WuJun,WANG ZiLong,ZENG ZhiJiang
    Scientia Agricultura Sinica. 2021, 54(11):  2464-2475.  doi:10.3864/j.issn.0578-1752.2021.11.017
    Abstract ( 282 )   HTML ( 21 )   PDF (1321KB) ( 369 )   Save
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    【Objective】 In honeybees, methyl palmitate (MP), methyl oleate (MO), methyl linoleate (ML) and methyl linolenate (MLN) are important capping pheromone components, which trigger the capping behavior of adult workers. The objective of this study is to compare the contents of these four pheromone components in the larvae of workers and drones of Apis cernana at different capping stages, analyze their biosynthetic pathways, and to further explore the mechanism of pheromone communication between larvae and adult workers. 【Method】Using A. c. cernana as the experimental material, the larvae of workers and drones of prior to be capped, in the process of being capped and had been capped were collected for comparing the contents of these four pheromone components by using GC/MS. Simultaneously, RNA-seq was used for gene expression analysis, and the biosynthetic pathways were speculated based on KEGG enrichment of differential expressed genes. 【Result】In worker larvae, the contents of the four capping pheromone components were significantly higher at the capping and capped stage than those of the prior to be capped larvae, and the contents of MP and MO significantly increased with aging of the larvae, while the contents of ML and MLN were not significantly different between the capping and capped stage. Whereas in drone larvae, the contents of the four pheromone components were higher overall and increased with aging, and the content at capped stage was significantly higher than that at prior to be capped and capping. RNA-seq results showed that there were 4 299 and 3 926 differential expressed genes among the larvae groups of three stages of workers and drones, respectively. In addition, 152 and 130 KEGG pathways were obtained from the KEGG annotation analysis of the differential expressed genes, respectively. Furthermore, the possible de novo biosynthetic pathways were proposed for MP, MO, ML and MLN from acetyl-CoA, regulating under 11 related candidate genes, and these biosynthesis pathways were found to be similar to those of Apis mellifera. 【Conclusion】The release contents of MP, MO, ML and MLN were increased during the critical stage of capping in worker and drone larvae of A. cernana, which further verified that these four pheromones were related to capping behavior of honeybees, and it was speculated that they were possibly de novo biosynthesized from acetyl-CoA under the control of related genes. A. cernana larvae and A. mellifera larvae may use the same biosynthesis pathway for pheromone biosynthesis.

    RESEARCH NOTES
    Analysis of Fruit Aromatic Components of Ten Plum Germplasm Resources in Northeast China
    WANG ShanShan,ZHAO ChenHui,LI HongLian,ZHANG BingBing,LIANG YingHai,SONG HongWei
    Scientia Agricultura Sinica. 2021, 54(11):  2476-2486.  doi:10.3864/j.issn.0578-1752.2021.11.018
    Abstract ( 366 )   HTML ( 25 )   PDF (1304KB) ( 412 )   Save
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    【Objective】In order to identify excellent plum resources with attractive aroma in Northeast China and to analyze the characteristic aromatic components for the plum fruits, ten plum germplasm resources from Northeast China were utilized to analyze their aromatic components and to identify characteristic aromatic components in their plum fruits, so as to provide reference for further molecular research and breeding application of plum aroma. 【Method】The aromatic components were determined using headspace solid phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Odor-activity values (OAV) and principal component analysis (PCA) were used to identify and analyze the characteristic aromatic components in the plum fruits. 【Result】A total of 63 aromatic components were identified in these ten plums. Among them, nine common aromatic components were detected in each of the ten plums, including (E)-2-hexenal, (E)-2-octenal, (E)-2-nonenal, decanal, 2-nonanone, 2,6,6-trimethyl-2-cyclohexene-1,4-dione, 2,4-di-tert-butylphenol, p-cymene, and (+) dipentene. The main aromatic components of Muhongtianli, Suilinghong, Yuejinli, Hongganhe, Huangganhe, Fortune, Great Rose, and Sifengli were aldehydes, whereas the main aromatic components of Xiangjiaoli and Niuxinli were esters. The PCA results of aromatic components showed that ten plums could be divided into three groups: the first group consisted of seven plum resources, including Muhongtianli, Suilinghong, Yuejinli, Hongganhe, Huangganhe, Fortune, Great Rose, and Sifengli; the second group consisted of only one plum resources, Xiangjiaoli, the third group was composed of only one plum, Niuxinli. According to the results of OAV values, the common characteristic aromatic components in all ten plums were (E)-2-hexenal and 2-nonanone. Other characteristic aromatic components were also detected in these plums: the characteristic aromatic components which could be detected only in Niuxinli were ethyl butyrate, ethyl caprate and isoamyl acetate; the characteristic aromatic components detected only in Xiangjiaoli were butyl acetate and hexyl propionate; the characteristic aromatic component detected only in Muhongtianli was α-pinene; the characteristic aromatic component detected only in Suilinghong was (E)-2-heptenal. 【Conclusion】β-Ionone was a characteristic aromatic component that could be detected only in seven plums distributed in Northeast China, including Muhongtianli, Suilinghong, Yuejingli, Hongganhe, Huangganhe, Xiangjiaoli, and Sifengli. Among the ten plum resources studied here, Muhongtianli, Niuxinli, and Xiangjiaoli had rich characteristic aromatic components, providing excellent plum resources with aroma.