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Table of Content

    01 October 2020, Volume 53 Issue 19
    CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS
    Allelic Variations of Pins Genes in Xinjiang Spring Wheat Varieties and Their Influence on Processing Quality of Xinjiang Hand-Stretched Noodles
    XIANG JiShan,LIU PengPeng,SANG Wei,CUI FengJuan,HAN XinNian,NIE YingBin,KONG DeZhen,ZOU Bo,XU HongJun,MU PeiYuan
    Scientia Agricultura Sinica. 2020, 53(19):  3857-3866.  doi:10.3864/j.issn.0578-1752.2020.19.001
    Abstract ( 387 )   HTML ( 56 )   PDF (422KB) ( 295 )   Save
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    【Objective】The objectives of this study were to detect the allelic variations of Pins genes in Xinjiang spring wheat varieties, analyze the difference of grain hardness among different Pins genotypes of spring wheats, and explore the effects of Pins genes on different quality characters and the processing quality of Xinjiang hand-stretched noodles.【Method】First, the allelic variations of Pins genes in 386 Xinjiang spring wheats varieties were detected with molecular markers. Second, the quality characters of these materials were determined, including milling quality, gluten quality, dough character, and starch gelatinization character. Third, the processing qualities of Xinjiang hand-stretched noodles were evaluated. 【Result】The allelic variations of Pins genes: In Xinjiang spring wheat varieties, there were two alleles (Pina-D1a and Pina-D1b) at Pina locus with proportions of 86.79% and 13.21%, respectively, three alleles (Pinb-D1a, Pinb-D1b, Pinb-D1p) at Pinb locus with proportions of 64.77%, 32.12%, and 3.11%, respectively, and six genotype combinations (Pina-D1a/Pinb-D1a, Pina-D1a/Pinb-D1b, Pina-D1a/Pinb-D1p, Pina-D1b/Pinb-D1a, Pina-D1b/Pinb-D1b, Pina-D1b/Pinb-D1p) for Pina/Pinb with proportions of 58.81%, 25.39%, 2.59%, 5.96%, 6.74%, and 0.52%, respectively. The effect of Pins gene on the quality characters of Xinjiang spring wheat: the grain protein content, and flour ash content, whiteness, and wet gluten content, Zeleny sedimentation value with Pina-D1a were significantly higher than those with Pina-D1b (P<0.05), whereas the grain hardness, flour yellowness (b*), gluten index, mid line peak time, 8 min integral, and starch breakdown of Pina-D1a were significantly lower than those of Pina-D1b (P<0.05). Among different alleles of Pinb, the flour whiteness and weak gluten content of Pinb-D1a, the grain flour yield and flour yellowness (b*) of Pinb-D1b, and the grain hardness and flour gluten index of Pinb-D1p were significantly higher than those of other alleles (P<0.05). Among different genotype combinations of Pina/Pinb, the flour whiteness of Pina-D1a/Pinb-D1a, the grain hardness, flour gluten index, and dough 8 min integral of Pina-D1a/Pinb-D1p, the starch breakdown of Pina-D1b/Pinb-D1b, and the grain protein content, flour yield, and flour wet gluten content of Pina-D1b/Pinb-D1p were significantly higher than those of other genotype combinations (P<0.05 or P<0.01). The effect of Pins genes on processing qualities of Xinjiang hand-stretched noodles: the stretch feeling, viscoelasticity, and total score of Pina-D1a were significantly lower than those of Pina-D1b (P<0.01). The stretch feeling of Pina-D1b/Pinb-D1p was significantly higher than those of other genotype combinations (P<0.01). The viscoelasticity of Pina-D1a/Pinb-D1p, Pina-D1b/ Pinb-D1a, and Pina-D1b/Pinb-D1b were significantly higher than those of other genotype combinations (P<0.05). The total score of Pina-D1b/Pinb-D1a and Pina-D1b/Pinb-D1b were significantly higher than those of other genotype combinations (P<0.01). 【Conclusion】 The mutation of Pina gene can significantly (P<0.05) increase the grain hardness and the total score of Xinjiang hand-stretched noodles. But the mutation of Pinb gene had no significant effect on the processing qualities of Xinjiang hand-stretched noodles. Pina-D1b/Pinb-D1a and Pina-D1b/Pinb-D1b are the key genotype combinations of high quality breeding for Xinjiang hand-stretched noodles.

    Establishment of Evaluation System for Drought Tolerance at Maize Germination Stage Under Soil Stress
    ZHANG ChunXiao,LI ShuFang,LIU XuYang,LIU Jie,LIU WenPing,LIU XueYan,LI ChunHui,WANG TianYu,LI XiaoHui
    Scientia Agricultura Sinica. 2020, 53(19):  3867-3877.  doi:10.3864/j.issn.0578-1752.2020.19.002
    Abstract ( 408 )   HTML ( 36 )   PDF (1319KB) ( 363 )   Save
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    【Objective】The main maize-producing areas in China are mostly arid and semi-arid areas, in which drought often occurred after sowing. The sufficient germination rate is key precondition for maize production. However, the decreasing germination rate caused by drought threatens the production of maize. Thus, it is of great importance to discover the drought tolerant maize varieties at germination stage, which relies on the establishment of evaluation system for drought tolerant maize at germination stage. 【Method】In the present study, 13 soil water content treatments, which represented the maize germination rates from 0 to 100%, were set using field soil and the germination rates of five representative maize hybrids were analyzed. The 50% germination rate soil water content (GW50) was determined based on the Logistic formula of germination rate and soil water content in five representative maize hybrids. Subsequently, 32 maize hybrids were treated with GW50 as drought condition. The relationships between germination rates of 32 hybrids and other 10 traits were investigated using correlation, cluster and principal component analyses. 【Result】The fitting degree (R2) of Logistic regression between germination rate of five representative maize hybrids and thirteen soil water contents ranged from 0.978 to 0.992. The GW50 of five representative hybrids, which were estimated by the Logistic regression formulas, ranged from 18.2%-18.7%, with mean of 18.5%. Under drought treatments of 18.5% soil water content, the germination rates of 32 hybrids showed significant correlation with germ fresh weight, radical fresh weight and storage material transport rate, but the correlation coefficients were all lower than 0.5, which was 0.36, 0.40 and 0.38, respectively. While the germination rates under GW50 showed no significant correlation with other seven traits such as radicle number. Besides, cluster analysis based on 10 other traits divided 32 hybrids to three clusters, each containing thirteen, ten and nine hybrids, respectively. However, the mean germination rates of the three clusters showed no significant difference. PCA analysis revealed germination rate, germ dry weight and storage material transport rate had highest eigenvalue in first principal component, which could explain 36.3% of the total variance. These three traits could be considered as key targets for drought tolerance evaluation at germination stage. Using the soil drought method and key traits for evaluation of drought tolerance at germination stage, four, twenty-two and seven of the thirty-four hybrids were identified as drought tolerant, intermedium and drought susceptible types, respectively. 【Conclusion】In this study, soil drought treatment method of GW50 was established for maize drought tolerance evaluation at germination stage. The germination rate, germ dry weight and storage material transport rate were identified as the key traits for high-throughput drought tolerance evaluation. Thus, an evaluation system for drought tolerance at maize germination stage was established.

    An Examination on Breeding Status Quo of Chinese Tartary Buckwheat Varieties Based on the National Cross-Country Tests of Tartary Buckwheat Varieties in China over 12 Consecutive Years
    WU CaoYang,LIANG ShiHan,QIU Jun,GAO JinFeng,GAO XiaoLi,WANG PengKe,FENG BaiLi,YANG Pu
    Scientia Agricultura Sinica. 2020, 53(19):  3878-3892.  doi:10.3864/j.issn.0578-1752.2020.19.003
    Abstract ( 286 )   HTML ( 17 )   PDF (835KB) ( 259 )   Save
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    【Objective】Aimed at analyzing agronomic traits and yield changes of national Tartary buckwheat varieties in regional trials over 12 consecutive years, and discussing improvements and existing problems with Tartary buckwheat varieties in China, so as to provide basis for future genetic improvements in Tartary buckwheat in China.【Method】Depending on influences of environmental factors such as climate and geography on characteristics of Tartary buckwheat and in terms of southern and northern trial groups classified according to the geographical division standards of North and South China, the cluster analysis, correlation analysis and multiple regression were employed to analyze variations of Tartary buckwheat varieties in growing duration, plant height, branch and node numbers of main stem, grain weight per plant, 1000-grains weight and yield in the National Tests of Tartary Buckwheat Varieties of China in 12 years from 2003 to 2014 and that examined correlations between different traits of Tartary buckwheat in the northern and southern trial groups, and traits differences of Tartary buckwheat varieties developed by the different breeding units between the two trial groups.【Result】Over the 12 years in question, the yields of the Tartary buckwheat varieties in the northern and southern trial groups increased by 21% and 32%, with an average annual rate of increase of 1.8% and 2.6%, respectively. There were totally 42 buckwheat varieties developed by 19 breeding units of 9 provinces, Shaanxi, Gansu, Yunnan, Guizhou, Jiangxi, Shanxi, Sichuan, Hunan and Chongqing. Of these breeding units, there were 5 units in Yunnan, 4 units in Guizhou, 3 units in Shaanxi, 2 units in Gansu, Shanxi and in Sichuan, and 1 unit in Jiangxi, Hunan and Chongqing City. The tested varieties developed by twelve breeding units from Yunnan, Guizhou and Shaanxi, three major Tartary buckwheat producers of China, made up 59.5% of all the tested varieties. The cluster analysis of Tartary buckwheat varieties from the different provinces (regions) showed that when the relative genetic distance was 5, the northern and southern trial groups can be divided into 4 and 3 classes, respectively. Of the northern group, the varieties from Shaanxi, Hunan and Shanxi were grouped into one class, the varieties from Gansu, Guizhou, Jiangxi and Yunnan were grouped into one class, and the varieties from Chongqing and Sichuan were grouped into one class; and of the southern trial group, the varieties from Guizhou, Jiangxi, Yunnan and Chongqing were grouped into one class, the varieties from Shaanxi, Gansu and Shanxi were grouped into one class, and the varieties from Sichuan and Hunan were grouped into one class. Correlation analysis showed that there was significantly positive correlations between the plant weights and yields of the northern and southern trial groups; significantly positive correlations between the grain weight per plant and yields of the northern trial group and significantly positive correlations of the grain weights per plant and plant heights with the branch numbers of main stems of the southern trial group; and there were different correlations in the other traits between the north and south trial groups. Multiple regression analysis showed that the growing durations, branch numbers of main stems, grain weights per plant and 1000-grains weights of the Tartary buckwheat varieties of the northern group jointly contributed 53.0% to their yield variation, and the growing durations, branch and node numbers of main stems, grain weights per plant and 1000-grains weights of the Tartary buckwheat varieties in the southern group jointly contributed 61.4% to their yield variation.【Conclusion】The national Tartary buckwheat variety regional tests in 12 years from 2003 to 2014 showed that certain improvements were made in the yield and related traits of Tartary buckwheat varieties in the northern and southern trial groups, with their yields increased to a certain extent and their growing durations shortened, but with their other traits not much changed. Of all the breeding units in question, the varieties developed by the breeding units in Yunnan, Guizhou and Jiangxi had wider environmental adaptabilities than the varieties developed by the other units in the other provinces. It was still the main goal in current Tartary buckwheat variety improvement to develop Tartary buckwheat varieties with high and stably yields, stress resistances and other excellent agronomic traits. Correlation analysis showed that it was an important approach in Tartary buckwheat variety breeding to improve Tartary buckwheat in grain weight per plant and branch numbers of main stems, etc. It was the key problem to develop Tartary buckwheat varieties with high flavonoids, easy hulling, good shattering and lodging resistances and uniform maturities and good suitability for mechanized cultivation for Tartary buckwheat production. Identifying new Tartary buckwheat germplasm resources and excellent genes, strengthening researches and developments and applications of new technologies such as molecular design breeding, and improving Tartary buckwheat variety improvement technologies and levels were the important directions in Tartary buckwheat variety improvement.

    SPECIAL FOCUS: HIGH SOLAR AND HEAT RESOURCES EFFICIENCY OF WHEAT-MAIZE CROPPING SYSTEM
    Annual High Efficiency Utilization of Solar and Heat Resources of Winter Wheat and Summer Maize in Double Cropping System
    ZHAO Bin,LI ZongXin,LI Yong,LIU Peng,ZHANG JiWang,REN BaiZhao,GAO Yingbo,WANG XuQing,ZHANG Bin,LIU KaiChang,WANG FaHong
    Scientia Agricultura Sinica. 2020, 53(19):  3893-3899.  doi:10.3864/j.issn.0578-1752.2020.19.004
    Abstract ( 436 )   HTML ( 18 )   PDF (331KB) ( 458 )   Save
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    Analysis on Limiting Factors of Efficient Utilization of Winter Wheat and Summer Maize Farmland Resources
    LIU XiaoYu,ZHANG DouDou,JIAO JinYu,CHEN GuoQing,LI Yong
    Scientia Agricultura Sinica. 2020, 53(19):  3900-3914.  doi:10.3864/j.issn.0578-1752.2020.19.005
    Abstract ( 289 )   HTML ( 11 )   PDF (5770KB) ( 464 )   Save
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    【Objective】With the decrease of cultivated land area, the increase of population and the rapid development of social economy, increasing crop yield per unit area is an important way to improve the total grain yield and ensure the food security in China. It is of great significance to explore the way of high efficiency and limiting factors to improve the unit yield. 【Method】 In this study, winter wheat and summer maize were taken as the research objects. In four ecological regions of Shandong province (eastern Shandong, central Shandong, northwestern Shandong and southwestern Shandong), the simulation model and other methods were employed to study yield under different climate and cultivation management conditions in 2008-2017, to put forward quantitative evaluation indicators, to analyze and clarify the main limiting factors affecting resource utilization and yield.【Result】(1) In the growth season of winter wheat in the eastern Shandong, the influencing rates of light and temperature resource, variety, cultivation pattern, water resource and nitrogen resource were 16.96%, 20.68%, 1.39%, 60.97% and 0, respectively, those in central Shandong were 37.72%, 20.16%, 1.57%, 40.55% and 0, and those in northwestern Shandong were 17.90%, 19.11%, 1.20%, 61.79%, 0, respectively; Those in southwestern Shandong were 33.65%, 23.80%, 1.65%, 40.90% and 0 respectively. (2) In the growth season of summer maize in the eastern Shandong, the influencing rates of light and temperature resource, variety, cultivation mode, water resource and nitrogen resource were 49.11%, 9.07%, 10.64%, 31.18% and 0, respectively; In central Shandong, the influence rates of each limiting factor were 56.62%, 10.86%, 11.65%, 20.87% and 0, respectively; In northwestern Shandong, the influence rates of each factor were 43.01%, 18.95% 11.26%, 26.78% and 0; Those in southwestern Shandong were 64.42%, 5.44%, 15.84%, 14.30% and 0, respectively. 【Conclusion】 In the winter wheat growing season, the farmland resources in the eastern and northwestern Shandong were mainly limited by water resource, followed by light and temperature resource and variety, and the cultivation mode and nitrogen resource had little influence. In the central and southwestern Shandong, it was mainly affected by light and temperature resource and water resources, followed by the variety factors. The growth season of maize in the four regions was influenced by light and temperature resource, followed by water resource, and less by varieties and cultivation patterns.

    Effects of Line-Spacing Expansion and Row-Spacing Shrinkage on Population Structure and Yield of Summer Maize
    DING XiangPeng,BAI Jing,ZHANG ChunYu,ZHANG JiWang,LIU Peng,REN BaiZhao,ZHAO Bin
    Scientia Agricultura Sinica. 2020, 53(19):  3915-3927.  doi:10.3864/j.issn.0578-1752.2020.19.006
    Abstract ( 288 )   HTML ( 15 )   PDF (510KB) ( 360 )   Save
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    【Objective】 The aim of this study was to explore the regulatory effects of expanding and shrinking cultivation models under different densities on the yield and population structure of Huang-Huai-Hai summer maize. 【Method】 The high yield maize variety Zhengdan958 was used as experimental material, three kinds of row spacing treatments, such as 60 cm (B1), 80 cm (B2), and 100 cm (B3), and two planting densities of 67 500 plants/hm2and 82 500 plants/hm2, were used to form different cultivation patterns through split zone design in 2018 and 2019. 【Result】 Compared with D1 density, D2 density could significantly increase the population leaf area and photosynthetic potential, improve the light energy utilization of the population, increase the dry matter accumulation of the population, and promote the increase of yield. Under the condition of different planting density, the effect of expansion and shrinkage on population structure was different. Under the density of 67 500 plants/hm2, the effect of expansion and shrinkage on the yield was not significant. Under the density of 82 500 plants/hm2, B2 treatment increased the yield by increasing the number of grains per row and 1000-grain weight, which was 9.45% and 11.48% higher than that of B1 and B3 treatments, respectively. B2 treatment significantly increased the population leaf area index (LAI), delayed the senescence of the middle and lower leaves, increased the photosynthetic potential of the population after anthesis, increased the angle between stems and leaves, and decreased the leaf orientation value. The light transmittance of leaf layer and bottom layer in panicle position increased significantly, the extinction coefficient decreased, the dry matter accumulation increased after anthesis, and the dry matter transfer decreased after anthesis. The results showed that under the condition of high density, the equal row spacing model of 80 cm expansion was beneficial to build an efficient photosynthetic population structure, delay leaf senescence, improve the photosynthetic performance of the population, increase the production and accumulation of dry matter of the population, and thus increase the yield. 【Conclusion】 The high-yield cultivation of summer maize in Huang-Huai-Hai Plain can achieve efficient utilization of light energy and synergistic increase in yield by increasing planting density and appropriate expansion and shrinking of plants. Under the experimental conditions, a planting pattern of 82 500 plants/hm2 with a density of 80 cm is recommended.

    Effects of Staggered Planting with Increased Density on the Photosynthetic Characteristics and Yield of Summer Maize
    ZHANG ChunYu,BAI Jing,DING XiangPeng,ZHANG JiWang,LIU Peng,REN BaiZhao,ZHAO Bin
    Scientia Agricultura Sinica. 2020, 53(19):  3928-3941.  doi:10.3864/j.issn.0578-1752.2020.19.007
    Abstract ( 319 )   HTML ( 22 )   PDF (880KB) ( 316 )   Save
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    【Objective】Increasing density is one of the important ways to increase the yield of maize, but with the increase of planting density, it will usually cause the colony closure and the decrease of the utilization rate of light energy. Therefore, it is very important to explore and change the planting mode to weaken the negative effects of excessive density on plants and to improve the group canopy structure.【Method】The experiment was conducted in 2018 and 2019. Using Denghai 605 and Zhengdan 958 as test materials, two planting modes of parallel planting and staggered planting were set up under the two density conditions of 67 500 plants/hm2 and 82 500 plants/hm2. The effects of density on summer maize yield and photosynthetic characteristics were expected to explore and to understand the interaction mechanism of staggered planting and density, and to propose a suitable planting model for high-yield summer maize, which provided a certain theoretical basis for the scientific planting model of summer maize.【Result】Increased density reduced the uniformity of the population. The ear leaf net photosynthetic rate (Pn), photosynthetic key enzyme activities, and chlorophyll content were lower than those of low density, and the photosynthetic key enzyme activities decreased more with the growth period under high density. This meant that increasing the density would increase the leaf senescence rate, which was not conducive to plant photosynthesis. Under the staggered planting model, the group uniformity was improved, the angle between stems and leaves was increased, the leaves were more flat, the light energy interception rate was increased, the activity of Pn, photosynthetic key enzymes and chlorophyll content increased, the accumulation of dry matter in the population and the distribution of dry matter to the grain increased, thereby significantly increasing the yield. Staggered planting increased the average yield by 3.8%-6.1% compared to planting of the parallel plant. Staggered planting under the premise of ensuring the number of groups weakened the competition between individual plants within the group for light and temperature resources, ensured the full development of the individual development potential of maize, and enabled the coordinated development of maize groups and individuals.【Conclusion】Staggered planting could significantly improve the group canopy structure, optimize the group’s lighting conditions, enhance its photosynthetic performance and material production capacity, and increase maize yield. Under the conditions of this experiment, comprehensive analysis believed that the stagger planting model under 82 500 plants/hm2 density conditions performs was the best, which could provide a reference for the establishment of a high-yield model of summer maize.

    Effects of Row Spacing and Mulching Reflective Film on the Yield and Light Utilization of Summer Maize
    BAI Jing,ZHANG ChunYu,DING XiangPeng,ZHANG JiWang,LIU Peng,REN BaiZhao,ZHAO Bin
    Scientia Agricultura Sinica. 2020, 53(19):  3942-3953.  doi:10.3864/j.issn.0578-1752.2020.19.008
    Abstract ( 263 )   HTML ( 25 )   PDF (589KB) ( 341 )   Save
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    【Objective】In recent years, it is the limitation for summer maize to face the less light radiation during the growing season, and the light deficiency has become the main limiting factor affecting the production of maize. In this study, the row spacing under high density of maize was adjusted appropriately to improve the light in the canopy and the interior of the population and to alleviate the influence of shading caused by high density, so as to lay a foundation for further improving the yield.【Method】This experiment took Zhengdan 958 as the experimental material under field conditions in 2018-2019, and there were two different planting densities, which were 67 500 plants/hm2 and 82 500 plants/hm2, and three row spacing forms (60+60, 80+40, 100+20), with mulching reflective film (FM) and no film treatment (NM). The effects of mulching reflective film and row spacing on light energy utilization and yield of summer maize were studied. 【Result】As the density increases, the maize yield increased. There was no significant difference in the yield of “60+60” and “80+40” row spacing configurations when the density of 67 500 plants/hm 2 was higher than that of “100+20” row spacing configurations under the condition of mulching reflective film and no film. But at 82 500 plants/hm 2 density, the row spacing configuration of “FM80+40” under the mulching treatment could effectively improve the light environment within the population and made the distribution of light energy in the canopy more reasonable, and significantly increase the dry matter accumulation, LAI, canopy light interception rate, Pn and chlorophyll content; Compared with “FM60+60” and “FM100+20” treatments, the two-year average yield of which were increased by 6.6% and 10.8%, respectively. The yield of “NM80+40” row spacing was 5.8% and 8.7% higher than that of “NM60+60” and “NM100+20” row spacing, respectively, and the yield of “FM80+40” was 5.1% higher than that of “NM80+40”.【Conclusion】Under the density of 82 500 plants/hm 2, “80+40” row spacing arrangement supplemented by mulching reflective film could significantly improve the photosynthetic characteristics of summer maize, increase the reflectance of transmitted light at the lower part, and then increase the illumination of the middle and lower part of leaves, prevent premature aging of leaves, and improve photosynthetic capacity. Therefore, this treatment was an ideal cultivation mode to achieve high yield of summer maize

    Effects of Pre-Silking High Temperature Stress on Yield and Ear Development Characteristics of Different Heat-Resistant Summer Maize Cultivars
    GAO YingBo,ZHANG Hui,SHAN Jing,XUE YanFang,QIAN Xin,DAI HongCui,LIU KaiChang,LI ZongXin
    Scientia Agricultura Sinica. 2020, 53(19):  3954-3963.  doi:10.3864/j.issn.0578-1752.2020.19.009
    Abstract ( 391 )   HTML ( 17 )   PDF (1771KB) ( 550 )   Save
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    【Objective】Pre-silking high temperature is likely to cause large negative impacts on maize yield, which is one of the important factors affecting ear development. This study was aimed to clarify the influence of pre-flowering high temperature on grain yield and ear development process, which was one of great significance for the stable and high yield of maize. 【Method】In this study, heat-resistant maize varieties Zhengdan958 and heat-sensitive maize varieties Lianchuang808 at flowering stage were used as research materials in artificial intelligence greenhouse, and then the influence of different high temperature of 40/30℃ and 35/25℃ on grain yield, ear development, ultrastructure of pollen and filament and photosynthetic characteristics from V9 to silking period were investigated.【Result】High temperature stress from V9 to silking period reduced the ear length, grain number and kernel weight of different genotypes summer maize, which led to a significant decrease in yield. Compared with control (35/25℃), the row grain number of Zhengdan 958 and Lianchuang 808 under high temperature significantly decreased by 22.21% and 24.59%, respectively; The kernel number per ear decreased by 29.85% and 27.80%, respectively; The thousand kernel weight decreased by 24.04% and 17.47%, respectively; The grain yield decreased by 44.98% and 40.88%, respectively. The dry weight of tassel, dry weight of ear, ear length and net photosynthetic rate of Zhengdan958 and Lianchuang808 under high temperature stress from V9 to silking period were significantly decreased 39.42% and 15.60%, 22.50% and15.56%,48.70% and 56.48% compared with control (35/25℃), respectively. The anthesis silking interval (ASI) of Zhengdan958 and Lianchuang808 increased to 7 d and 6 d as a result of delay of silking period rather than tasseling period. High temperature stress had obvious influence on the ultrastructure of maize pollen and filament surface of two maize varieties. Under high temperature stress, the surface of the pollen grain shriveled and collapsed, net vein protuberance and collapsed germinal aperture. At the same time, the filament surface shrank horizontally, the number of filament hair significantly reduced, and almost all residual filament hair lodged on the surface of the filament, which reduced the filament area of accepting the pollen.【Conclusion】High temperature stress from V9 to silking period were more serious on yield formation, photosynthetic characteristics and ear development of Zhengdan958 than Lianchuang808. High temperature stress from V9 to silking period significantly damaged the pollen and filament morphology, inhibited the development of tassel and ear, reduced the photosynthetic capacity, and decreased the kernel number per ear and kernel weight of two maize varieties, which significantly reduced the grain yield of maize. Therefore, the selection of maize varieties in field depended on the period of high temperature stress.

    PLANT PROTECTION
    Secondary Metabolites from a Marine-Derived Fungus Aspergillus versicolor and Their Anti-Phytopathogenic Bacterial Activity
    FU Bing,WANG Mei,LIU JianYang,LIN Wei,ZHANG ChengSheng,ZHAO DongLin
    Scientia Agricultura Sinica. 2020, 53(19):  3964-3974.  doi:10.3864/j.issn.0578-1752.2020.19.010
    Abstract ( 449 )   HTML ( 8 )   PDF (534KB) ( 530 )   Save
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    【Objective】 Plant bacterial diseases are becoming more and more serious, and lack of control pesticides. The objective of this study is to isolate bioactive compounds from the previously obtained marine-derived fungus Aspergillus versicolor D5 with antibacterial activity and abundant secondary metabolites, identify their chemical structures, evaluate their anti-phytopathogenic bacterial activity, so that to clarify the antibacterial components of the target fungus. This research will provide lead compounds for the discovery of new antibacterial pesticides.【Method】The compounds were isolated by silica gel column chromatography (CC), octadecylsilyl silica gel CC, Sephadex LH-20 CC, and semipreparative HPLC, and were identified by modern spectral analysis methods including NMR and MS spectra. In addition, the actibacterial activity of the isolated compounds towards six phytopathogenic bacteria, including Acidovorax avenae, Erwinia carotovora, Clavibater michiganensis, Pseudomonas syringae, Ralstonia solanacearum and Xanthomonas campestris, was evaluated by double dilution method to obtain the minimum inhibitory concentration (MIC).【Result】Twelve compounds were isolated and identified from the ethyl acetate extract of the marine-derived fungus A. versicolor D5 fermented by potato dextrose water media, including four 4-aryl-quinolin-2-one alkaloids, viridicatin (1), 3-O-methylviridicatin (2), 3,6-O-dimethylviridicatin (3), and 3-O-methylviridicatol (4), two dioxopiperazine alkaloids, (+)-cyclopenol (5) and (-)-cyclopenol (6), three fumiquinazolines alkaloids and their derivatives, versicoloid A (7), chrysopiperazine C (8), and cottoquinazoline A (9), and three anthraquinone derivatives, versiconol (10), averufin (11), and noraverufanin (12). Among them, a 4-aryl-quinolin-2-one alkaloid, 3,6-O-dimethylviridicatin (3) exhibited an obvious antibacterial activity against R. solanacearum and X. campestris with MIC values of 50 and 100 μg·mL-1, respectively. The structure activity relationship analysis revealed that the methoxy group at C-6 might play an important role in anti-phytopathogenic bacterial activity.【Conclusion】The metabolites of A. versicolor D5 are abundant, which can produce alkaloids and anthraquinones with various structures. Among them, nine alkaloids and three anthraquinones were obtained from cultures of D5. 3,6-O-dimethylviridicatin (3) exhibited clear antibacterial activities towards R. solanacearum and X. campestris.

    Research Progress and Prospect on Banker Plant Systems of Predators for Biological Control
    LI Shu,WANG Jie,HUANG NingXing,JIN ZhenYu,WANG Su,ZHANG Fan
    Scientia Agricultura Sinica. 2020, 53(19):  3975-3987.  doi:10.3864/j.issn.0578-1752.2020.19.011
    Abstract ( 434 )   HTML ( 13 )   PDF (717KB) ( 377 )   Save
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    The impact of intensive agriculture on the ecological environment is growing, which fosters singular landscape, loss of biodiversity and prone to pest outbreaks. With the national development strategy of modern agriculture, much attention has been paid to the production of healthy, sustainably grown food. The requirement for environmentally friendly and efficient pest management technologies has become more and more intensified. Natural enemies play an important role in ecological security and sustainable agriculture. However, the traditional method of mass releasing natural enemies has the problems of high cost, poor timeliness and efficiency. So how to protect natural enemies in the field and improve efficiency are the critical problems to successful biological control. Banker plant systems have the advantages of introducing natural enemies preventively and maintaining them, therefore, controlling the pests sustainably. It is a relatively successful protective biological control technology, which has improved the survival conditions of natural enemies in the comprehensive management of agricultural pests. In recent years, with the rapid development of banker plant systems, more and more products have been widely applied in Belgium, Germany, France, Japan, the United States, Canada, and so on. However, due to the geographical differences, the direct application of the banker plant systems reported abroad is difficult in most cases. At present, although the domestic study started late, it has reached a rapid development period and will have a broad development prospect. In this paper, the research progress and the related technology in China and abroad were summarized. Especially, the optimal strategy of predatory natural enemies was discussed in banker plant system. The improvement of the technology not only needs careful selection of the factors, but also clarifies the relationship and optimizes the levels of the elements. Moreover, fully considering is in the layout of space and time, especially the application effect evaluation in the field. That will improve the banker plant system technology. And the prospect of the development direction in the future was pointed out. Based on principles of adjusting measures to local conditions, the advanced banker plant system would surely promote the widespread application and industrialization of predatory natural enemy products.

    The Persistent Infection and Detection of Cnaphalocrocis medinalis Granulovirus in Cnaphalocrocis medinalis
    HAN GuangJie,LIU Qin,LI ChuanMing,QI JianHang,XU Bin,LU YuRong,XU Jian
    Scientia Agricultura Sinica. 2020, 53(19):  3988-3995.  doi:10.3864/j.issn.0578-1752.2020.19.012
    Abstract ( 298 )   HTML ( 12 )   PDF (1740KB) ( 174 )   Save
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    【Objective】Cnaphalocrocis medinalis granulovirus (CnmeGV) is a specific pathogenic microorganism of Cnaphalocrocis medinalis. Persistent infection of baculovirus plays an important role in pest population control. The objective of this study is to construct the detection method of persistent infection with CnmeGV, analyze the control effect of viral persistent infection on pest population, and to provide a theoretical basis for the application of viral pesticides.【Method】The largely diverged regions were selected to design CnmeGV nested PCR primers based on multiple comparisons of granulin gene sequences of granulovirus. The sensitivity and reliability of probes were further evaluated. The maize leaves were used to feed C. medinalis in the laboratory. The 4th instar larvae were singled out, and then fed with maize leaves soaked by CnmeGV with a sublethal concentration of 106 OB/mL. The infected C. medinalis were reared to adult stage, and the emergence rate was counted. After treating the insect body surface with 10% formaldehyde, the carrying CnmeGV rates of larvae, pupae, pupal molts and adults in persistent infection population of C. medinalis were analyzed using these probes. The prevalence rate of larvae and carrying rate of soil in the next year after using CnmeGV were also detected.【Result】The probes of CnmeGV nested PCR were constructed including the outer primer Cm-gran1 and the inner primer Cm-gran2. The sensitivity of nested PCR was 0.85 fg·μL -1 genomic DNA, which was 1 000 times higher than that of the conventional PCR. The probes were highly reliable, and no target fragment was detected in the food sources of C. medinalis (rice and maize), other polyhedrosis and granulosis viruses. The target fragments of CnmeGV were not detected in larvae after treated with 10% formaldehyde for 10, 30 min and 16 h, respectively. However, the pupa of C. medinalis could not emerge after 16 h of treatment. The detection rate of CnmeGV was 100% in the larvae of infected population treated with sublethal concentration of CnmeGV for 96 h. After pupation and emergence, the detection rates of pupae, pupal molts and adults were 87.5%, 83.3% and 16.7%, respectively. The detection rate of CnmeGV had no significant change from larva to pupa stage (χ 2=3.2, P=0.234) by Chi-square test. However, the detection rate of CnmeGV decreased significantly (χ 2=32.356, P=0) from pupa to adult stage, indicating that most of the viruses were excreted from the body with pupal molt during metamorphosis. The emergence rate of adult in infected population (30.8%) was significantly lower than that of the control (93.4%). Field tests showed that the prevalence rate of C. medinalis larvae in the field at one year following CnmeGV treatment was 4%, and the rate of soil carrying CnmeGV was 58%, suggesting that CnmeGV could survive in the soil and continue to infect C. medinalis through horizontal transmission.【Conclusion】The sensitivity of constructed nested PCR probes is high and can be used for the detection of CnmeGV persistent infection. Persistent infection of CnmeGV can effectively control the population development of C. medinalis, and adult metamorphosis plays an important role in removing pathogens.

    SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT
    Evaluation of Nitrogen Supply Capacity of Paddy and Wheat Rotation Soil in Hanzhong Basin by Different Determination Methods
    ZHANG FangFang,MA NingBo,YUE ShanChao,LI ShiQing
    Scientia Agricultura Sinica. 2020, 53(19):  3996-4009.  doi:10.3864/j.issn.0578-1752.2020.19.013
    Abstract ( 241 )   HTML ( 9 )   PDF (1557KB) ( 249 )   Save
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    【Objective】 The different indexes were compared to evaluate the reliability of nitrogen (N) supply capacity of soil in Hanzhong basin, so as to provide references for local soil N management.【Method】Soil samples were collected from 12 farmlands in Hanzhong basin and the surrounding hilly areas. The cumulative N uptake of potted ryegrass was used as a reference. Soil physical and chemical properties parameters were used as the indexes of soil N supply capacity, which included soil N mineralization amount based on three chemical methods (mineral N method, KCl condensation and reflux acid potassium permanganate method) and two biological methods (aerobic incubation and waterlogged incubation). 【Result】Soil type was an important factor that affected the N supply capacity of soil. Total N or organic matter could reflect the potential N supply capacity. However, soil texture, pH, available phosphorus (Ava.P), cation exchange capacity (CEC), calcium carbonate and particle composition (sand, silt and clay) could not reflect the N supply capacity. The correlation coefficient between aboveground N uptake of ryegrass and N value by mineral N method was 0.963 (P<0.01). However, since the initial mineral N could not reflect the amount of organic N mineralization, the mineral N method could only reflect the current N supply capacity, so it was not suitable as an evaluation index of soil N supply capacity. The correlation coefficient between aboveground N uptake of ryegrass and total mineral N measured by KCl reflux condensation method was 0.912 (P<0.01), while the correlation coefficient between aboveground N uptake of ryegrass and the amount of mineralizable N measured by KCl condensate reflux method was -0.766 (P<0.01). Because the leaching process of soil mineralizable N by KCl refluxing method led to the volatilization of ammonium N, which might result in the inconsistency in reflecting the potential N supply capacity and the total N supply capacity, so KCl refluxing method was not an ideal indicator to reflect the soil N supply capacity of Hanzhong basin. The correlation coefficients of total mineral N and mineralizable N with aboveground N uptake of ryegrass were 0.847 and 0.833 (P<0.01), respectively, which could reflect both the potential N supply capacity and the total N supply capacity, and it was the best chemical method. Under the condition of aerobic incubation, total mineral N and mineralizable N were not correlated with aboveground N uptake of ryegrass. While under the condition of waterlogged incubation, the correlation coefficients of total mineral N and mineralizable N with aboveground N uptake of ryegrass were 0.921 and 0.890 (P<0.01), respectively, indicating that the waterlogged incubation method could reflect the potential N supply capacity and total N supply capacity of paddy and wheat rotation soil in Hanzhong basin, and it was a good biological incubation method. The correlation coefficients of N0 and initial mineral N + N0 with aboveground N uptake of ryegrass in the first four stages were 0.834 and 0.845(P<0.01), respectively. The correlation coefficients with N uptake of the whole ryegrasses were 0.840 and 0.851(P<0.01), respectively. Both N0 and initial mineral N + N0 could reflect the potential N supply capacity. But N0 could only reflect the potential N supply capacity, while initial mineral N + N0 could reflect the potential N supply capacity and total N supply capacity. Therefore, initial mineral N + N0 was an ideal index.【Conclusion】For the evaluation of N supply capacity of rice-wheat rotation soil in Hanzhong basin, the acid potassium permanganate method was the best chemical method, and the waterlogged incubation method was a good biological incubation method. The initial mineral N + N0 was an ideal indicator to reflect the N supply capacity of soil in Hanzhong basin.

    Potential of Fertilizer Reduction and Benefits of Environment and Economic for Cereal Crops Production in Shaanxi Province
    ZHANG XinXin,SHI Lei,HE Gang,WANG ZhaoHui
    Scientia Agricultura Sinica. 2020, 53(19):  4010-4023.  doi:10.3864/j.issn.0578-1752.2020.19.014
    Abstract ( 278 )   HTML ( 7 )   PDF (3446KB) ( 362 )   Save
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    【Objective】The management of farmers is the main mode of agricultural production in China, and the unreasonable application of fertilizer resources is the crucial factor in limiting the production of crops. This study was aimed to assess the potential of fertilizer reduction and benefits of environment and economic, which was very important for agricultural sustainable development in the main crop production regions.【Method】In 2018, a questionnaire survey was conducted on the production of major crops in counties (cities and districts) of Shaanxi Province. The three major crops were taken as research objects to evaluate farmers’ fertilization status, fertilizer reduction potential, environmental costs and economic benefits based on yield level.【Result】There were significant differences in farmers’ crop yields. The average yield of wheat was 4 573 kg·hm-2, and the difference between high and low yield was 2 619 kg·hm-2; the average yield of maize was 7 319 kg·hm-2, and the difference between high and low yield was 5 388 kg·hm-2; the average yield of rice was 8 340 kg·hm-2, and the difference between high and low yield was 2 893 kg·hm-2. The nitrogen, phosphorus and potassium rate of wheat was 177 kg N·hm-2, 102 kg P2O5·hm-2 and 37 kg K2O·hm-2, respectively; maize was 247 kg N·hm-2, 103 kg P2O5·hm-2 and 47 kg K2O·hm-2; rice was 186 kg N·hm-2, 88 kg P2O5·hm-2 and 64 kg K2O·hm-2. There is no significant relationship between yield and fertilizer application. The problems of excessive application of nitrogen and phosphate fertilizer, excessive and insufficient potassium fertilizer coexist, and excessive fertilization in low-yield farmers was serious. The reduction potential of nitrogen, phosphorus, potassium in wheat was 41%, 59% and 59%, respectively; maize was 55%, 73% and 66%, respectively; for rice, it was 38%, 64% and 58%, respectively. The forms of fertilizers applied showed that nitrogen was mainly chemical fertilizers and supplemented by compound fertilizers; phosphorus and potassium were mainly compound fertilizers; the organic manure supplied very low nutrient. The application of basal fertilizer usually received more attention, while topdressing was often overlooked on fertilizer structure, nitrogen was mainly based on basal application and supplemented by topdressing; phosphorus and potassium fertilizers were rarely applied topdressing. Losses of reactive nitrogen to produce 1.0 t wheat, maize and rice were 6.9, 3.8 and 3.3 kg N, respectively. Compared with the high-yield group, the reactive nitrogen loss intensity of wheat, maize and rice in the low-yield group increased by 52%, 85% and 74%; the potential of loss reduction ranges of wheat, maize and rice in low-yield group were 16%-33%, 31%-50% and 4%-38%, respectively. The economic benefits of wheat, maize and rice production were 4 468, 9 091 and 20 020 yuan/hm2, respectively. Compared with the low-yield group, the economic benefits of wheat, maize and rice in high-yield group increased 455%, 128% and 52%, respectively. The benefit increases were 4 919, 9 905 and 20 543 yuan/hm2. Compared with the low-yield group, the benefit increases of wheat, maize and rice in high-yield group increased 290%, 106% and 48%, respectively.【Conclusion】The household production behavior was analyzed deeply based on the production level in Shaanxi province. The low and middle yield levels were the focus of fertilizer reduction and benefit improvement. The average reduction potential of nitrogen, phosphorus, and potassium fertilizers for the three crops was 45%, 65% and 61%, respectively, reducing the potential of active nitrogen loss were 26%, 45% and 18%, respectively. The potential to improve environment economic benefits were 10%, 9% and 3%, respectively.

    Effects of Successive Biochar Addition to Soil on Nitrogen Functional Microorganisms and Nitrous Oxide Emission
    DONG Cheng,CHEN ZhiYong,XIE YingXin,ZHANG YangYang,GOU PeiXin,YANG JiaHeng,MA DongYun,WANG ChenYang,GUO TianCai
    Scientia Agricultura Sinica. 2020, 53(19):  4024-4034.  doi:10.3864/j.issn.0578-1752.2020.19.015
    Abstract ( 321 )   HTML ( 15 )   PDF (1927KB) ( 407 )   Save
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    【Objective】In recent years, the biochar has been widely concerned due to its some positive roles in soil improvement, agricultural production, and carbon sequestration and emission reduction. The purpose of this experiment was to reveal the microbiological mechanism of biochar on crop yield and nitrous oxide (N2O) emission and to provide a theoretical basis for extending application of biochar in agriculture.【Method】A fixed field experiment with successive straw-based biochar amended at 0 (BC0, CK), 2.25 (BCL, low rate), 6.75 (BCM, medium rate), and 11.25 t·hm-2(BCH, high rate) was carried out in the typical farmland with fluvo-aquic soil in Huanghuai area of China. Effect of successive biochar addition after six year on nitrous oxide (N2O) emission, nitrogen (N) functional genes and grain yield of summer maize were studied by field observation, chemical analysis and real-time quantitative polymerase chain reaction (qPCR) in laboratory.【Result】Compared to BC0, biochar application significantly increased grain yield of summer maize with the maximum value (10 811 kg·hm-2) under BCM treatment, and also significantly reduced N2O emissions with the better performance for BCM during summer maize season. Compared with BC0, biochar application could significantly improve inorganic nitrogen storage and moisture content in topsoil. In addition, following biochar application rate, the soil AOA gene abundance increased first and then decreased with the highest gene copy number in BCM treatment at the bellmouth stage and maturity stage of summer maize, while the higher AOB gene abundance under BCH and BCM treatment presented respectively at the bellmouth stage and maturity stage of summer maize. Similar to nitrification function related genes (AOA and AOB), compared with BC0, the medium and high-rate biochar application (BCM, BCH) also significantly increased the copy number of denitrification function related genes (nirK, nirS, nosZ) at the bellmouth stage and maturity stage of summer maize. Correlation analysis indicated that the significantly negative correlation was found between N2O emissions, NO3-, moisture content (MO) and the expression of the AOA, AOB, nirK, nirS, and nosZ genes in topsoil at maturity of summer maize.【Conclusion】Successive biochar addition could reduce N2O emission by increasing the gene abundance of soil N transformation, and increase crop yield by improving inorganic nitrogen storage and moisture content in topsoil. Overall, the treatment with 6.75 t·hm-2 biochar led to the best comprehensive performance in the study region.

    HORTICULTURE
    Effects of Potassium Fertilizers Being Bag-Controlled Released on Fruit Yield and Quality of Peach Trees and Soil Chloride Content
    ZHANG YaFei,PENG FuTian,XIAO YuanSong,LUO JingJing,DU AnQi
    Scientia Agricultura Sinica. 2020, 53(19):  4035-4044.  doi:10.3864/j.issn.0578-1752.2020.19.016
    Abstract ( 288 )   HTML ( 8 )   PDF (474KB) ( 294 )   Save
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    【Objective】The effects of mixtures of potassium fertilizers being bag-controlled released on the photosynthesis, fruit yield and qualities of peach trees and the chloride content in soil were studied to provide reference for scientific application of potassium fertilizers in peach orchard.【Method】Late-ripening peach ‘Ruipan 21’/Prunus persica (Carr. ) Franch. were used as research materials, and a 2-year field trial was conducted with five types of fertilizers being bag-controlled released, including without potassium fertilizer (Control), 100% potassium sulfate (PC 0), 30% potassium chloride and 70% potassium sulfate (PC 30), 60% potassium chloride and 40% potassium sulfate (PC 60) and 100% potassium chloride (PC 100). It was fertilized at the beginning of March every year, the soil dynamic changes of available potassium and chloride content in 0-20 and 20-40 cm soil layer were determined in the middle of April, June, August and October of each year. The potassium and chloride content, SPAD values and photosynthetic rates in leaves were determined in the first rapid growth stage (S1), the hard core stage (S2), the second rapid growth stage (S3) and the maturity stage (S4) of peach trees, and the chloride content in fruit and the yield and qualities were investigated.【Result】The average Cl- content in the 0-20 and 20-40 cm soil layer was 34.03 and 38.78 μg·g-1 in the orchard without chlorine treatment. With the increase of potassium chloride, the Cl- content in different soil layers showed an increasing trend. The average content of Cl- in the 0-20 and 20-40 cm soil layer under PC 30, PC 60 and PC 100 were 37.98, 39.55, 41.61, 45.62, 51.17 and 58.87 μg·g-1, respectively. However continuous application of bag-controlled potassium chloride for two years did not result in the accumulation of Cl- in soil. The content of Cl- in the leaves also increased with the increase of the amount of chlorine applied. The content of Cl- under PC 30, PC 60 and PC 100 was 6.35%, 24.30% and 32.22% higher than that under PC 0. Among them, the content of Cl- in the leaves of the four stages treated by PC 30 was 234.29, 243.16, 233.81 and 233.20 μg·g-1, respectively. Moreover, PC 30 treatment significantly increased the SPAD value and net photosynthetic rate of leaves, while PC 60 and PC 100 treatment reduced the photosynthetic capacity of leaves. The content of available potassium in soil was higher in the early stage and decreased gradually later. The potassium release peak under PC 30, PC 60 and PC 100 treatments occurred in June, while the peak under PC 0 treatments occurred in August. The potassium content in the leaves reached the highest value in the second fruit growth stage (S 3), then decreased gradually, and the potassium content in the leaves at the maturity stage (S4) was the lowest. There was no significant change in the content of available potassium in leaves under different potassium treatments, indicating that the release rate of potassium chloride treatment had no significant effect on potassium absorption in leaves. The Cl- content between PC 30 and PC 0 fruits had no significant difference, with an average content of 55.0 μg·g-1. But the Cl- content increased by 10.40% and 28.45% under PC 60 and PC 100, compared with PC 0 on average. For two consecutive years, the single fruit weight and yield treated with PC30 increased slightly, and which of PC 60 and PC 100 treatments were significantly reduced. The application of low potassium chloride had no significant effect on fruit quality, however medium or high potassium chloride could reduce the fruit quality.【Conclusion】Using the method of fertilizer being bag-controlled release, replacing potassium sulfate with 30% potassium chloride resulted in no accumulation of Cl- in the soil, and could promote leaves photosynthesis, increase yield, and did not cause the decline of fruit quality and toxicity of the tree. Therefore, the proper amount of potassium chloride could be used instead of potassium sulfate in the peach orchard following the model of fertilizers being bag-controlled release.

    Screening and Inheritance of Fruit Storage-Related Traits Based on Reciprocal Cross of Southern×Northern High Bush Blueberry (Vaccinium Linn)
    LIU YouChun,LIU WeiSheng,WANG XingDong,YANG YanMin,WEI Xin,SUN Bin,ZHANG Duo,YANG YuChun,LIU Cheng,LI TianZhong
    Scientia Agricultura Sinica. 2020, 53(19):  4045-4056.  doi:10.3864/j.issn.0578-1752.2020.19.017
    Abstract ( 300 )   HTML ( 6 )   PDF (1423KB) ( 222 )   Save
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    【Objective】 This study was aimed to screen the diagnostic phenotypic traits for the storability of blueberry fruit and analyze their inheritance to assist the storage improvement breeding. 【Method】 Seven phenotypic traits were investigated, including stem scar, berry firmness, soluble solids content, acid content, fruit weight, fruit index and storability, among which the storability related traits was screened through correlation analysis and principal component analysis (PCA), and the inheritance was analyzed with reciprocal cross progenies.【Result】 The correlation analysis and PCA showed that stem scar was negatively correlated with fruit storability (P<0.001), but berry firmness was positively correlated with fruit storability (P<0.01). Both stem scar and berry firmness played major roles in the development of the storability of blueberry fruit. The storage test of fruit with and without stalk revealed that stem scar had a great influence on the storability of blueberry. The size of stem scar and berry firmness presented normal distributions in reciprocal populations with the coefficient of variation of more than 15%, suggesting that stem scar and berry firmness were quantitatively controlled by polygenes. The mean of stem scar in the reciprocal populations was similar to the mid-parental values, and its broad sense heritability (H2) in reciprocal populations were 0.887 and 0.867, respectively, indicating that the variations of stem scar mainly resulted from inheritance, and were mainly affected by additive effects. The mean of berry firmness in both reciprocal populations was prone to low value parent, and its broad sense heritability was 0.585 and 0.514, respectively in reciprocal populations, indicating that berry firmness was affected to a great extent by environmental effects. 【Conclusion】 The stem scar and berry firmness were the major traits for blueberry fruit storability. The inheritance of stem scar inclined to the mid-parental value, and its variation was mainly controlled with genetic effects, while berry firmness value tended to the low-value parent with large environmental effects.

    FOOD SCIENCE AND ENGINEERING
    Research Advance on the Application of Non-Saccharomyces in Winemaking
    ZHAN JiCheng,CAO MengZhu,YOU YiLin,HUANG WeiDong
    Scientia Agricultura Sinica. 2020, 53(19):  4057-4069.  doi:10.3864/j.issn.0578-1752.2020.19.018
    Abstract ( 644 )   HTML ( 36 )   PDF (522KB) ( 769 )   Save
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    Non-Saccharomyces yeasts have become a hot research topic in wine microbiology recently. They were used to be considered as spoilage microbes in winemaking, but now many studies have found that non-Saccharomyces yeasts could improve wine quality by enhancing the color stability, the aroma and the taste. In this work, the recent studies on the application of non-Saccharomyces yeasts in winemaking were reviewed, including the fermentation characteristics of popular non-Saccharomyces yeasts, the role of non-Saccharomyces yeasts in modulation of wine flavor, and the interaction between yeasts during fermentation. Different non-Saccharomyces yeasts have distinctive fermentation characteristics and thus affect the wine flavor differently when they are co-inoculated with Saccharomyces cerevisiae. Selection of appropriate non-Saccharomyces yeasts based on their fermentation characteristics is critical to produce the desired wine style. Non-Saccharomyces yeasts can affect the concentrations of alcohol, glycerol, volatile aroma compounds, mannoproteins/polysaccharides, anthocyanins and other substances in wine through various metabolic pathways, which is quite different from Saccharomyces cerevisiae. In addition to the wine flavor enhancement by non-Saccharomyces yeasts, the malolactic fermentation by Schizosaccharomyces pombe and the high efficiency lactic acid production by Kluyveromyces thermotolerans should also be paid attention for their applications in wine production. This paper summarized the research progress in the application of non-Saccharomyces yeasts in winemaking, in order to evaluate the role of non-Saccharomyces in wine production and provide valuable information on inoculation strategy for co-fermentation.

    ANIMAL SCIENCE·VETERINARY SCIENCE·RESOURCE INSECT
    A Review on Longevity Trait in Dairy Cattle Breeding
    ZHANG HaiLiang,LIU AoXing,MI SiYuan,LI Xiang,LUO HanPeng,YAN XinYi,WANG YaChun
    Scientia Agricultura Sinica. 2020, 53(19):  4070-4082.  doi:10.3864/j.issn.0578-1752.2020.19.019
    Abstract ( 516 )   HTML ( 16 )   PDF (569KB) ( 432 )   Save
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    Longevity is an important functional trait in dairy cattle. In addition to yield traits, longevity is more economically important than other traits in dairy breeding. The characteristics of longevity include low heritability, unfollowing normal distribution and performance later, hence longevity is the most difficult trait to select in dairy breeding. Since the 1950s, dairy breeders have begun to pay attention to longevity traits. Now, researches on longevity traits have continued. After the 1990s, longevity has been included in total selection index in many countries. However, longevity traits currently are not included in China Dairy Performance Index (CPI), and the study on longevity traits is also in its infancy in China. This review presented the trait definitions, methods of genetic evaluation, relationships between other traits, genetic makers, and selection strategies on longevity traits to systematically introduce its research and application in dairy industry. The complex relationships between longevity and other traits were described by summarizing the genetic correlation coefficients reported by many studies. The selection strategies for longevity traits in the various countries with developed dairy industry were highlighted by summarizing information collected from their dairy breeding program. Furthermore, this review also summarized the studies on longevity traits in dairy cattle in China. Longevity traits had various definitions, and there were various models used to preform genetic analysis, including linear model, threshold model, survival model and random regression model. There were low to moderate genetic correlations between longevity and other traits, including yield, linear type, fertility, health and workability traits, in which the higher genetic correlations were found between longevity and linear type traits in udder system. Generally, dairy cows with better performance on fertility and health traits were better on longevity. Among different populations, there were some differences on relationships between longevity and other traits, which were largely influenced by selection goals of current population. The total selection indexes in many countries included longevity. In addition to using direct longevity to select it, the indirect selection methods were also used in many countries. Udder system, strength, rump angle, feet and leg system, and mastitis resistance were comment traits used to indirect selection. Many genetic markers associated with longevity have been found in various populations, most of which were in genetic regions that have been reported to be associated with traits such as reproduction, disease and body type. Finally, this review also proposed the necessities of collecting longevity data, estimating genetic parameters, locating genetic markers, exploring evaluation model and selection strategy in China dairy cattle population.

    Pharmacokinetics of Chlortetracycline Microspheres in Pigs
    XU Ying,YAN ChangYan,YANG WeiCong,ZHANG YunXiao,YU Yang,HUANG XianHui
    Scientia Agricultura Sinica. 2020, 53(19):  4083-4091.  doi:10.3864/j.issn.0578-1752.2020.19.020
    Abstract ( 279 )   HTML ( 14 )   PDF (637KB) ( 171 )   Save
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    【Objective】A method was developed for the determination of chlortetracycline in swine plasma with high- performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The pharmacokinetics of chlortetracycline in pigs, including chlortetracycline hydrochloride, 10% chlortetracycline microspheres and 15% chlortetracycline microspheres were studied.【Method】Pharmacokinetics of chlortetracycline were investigated after intravenous and oral administration to pigs. Sixteen cross-bred pigs(Duroc×Landrace×Yorkshire) weighing (20±2.5) kg received a dose of 10 mg·kg-1 of chlortetracycline hydrochloride intravenously and 40 mg·kg-1 of 10% chlortetracycline microspheres and 15% chlortetracycline microspheres orally in both the fasted and the feeding condition in a three-way crossover design with one week washout period between each administration. Blood samples were collected in different time set before the experiment. The swine plasma samples were ultrasonic extracted with 0.1 mol·L-1 Na2EDTA-McIlvaine buffer. Then the extract was loaded on the HLB solid phase extraction (SPE) cartridge. After evaporation by nitrogen with water bath at 35℃, the extract was redissolved with methanol. Chlortetracycline was separated using a CNW C18 column with a mobile phase of acetonitrile -0.1% formic acid. The mobile phase was gradient elution at the flow rate of 0.25 mL·min-1. Chlortetracycline was analyzed by mass spectrometry equipped with electrospray ionization in the multiple reaction monitoring and positive ion mode and determined by an external standard quantitation. In the range of 5-500 ng·mL-1, the calibration curves of chlortetracycline from swine plasma showed good linearity (r>0.9990). The limit of detection (LOD) was 5 ng·mL-1 and the limit of quantification (LOQ) was 10 ng·mL-1. The average recovery of chlortetracycline was 76.90%-89.25% at low, medium and high concentration while the inter-day and intra-day coefficient of variations were 2.97%-9.45% and 6.16%-13.39%, respectively. The method was accurate, sensitive and suitable for the determination of chlortetracycline in swine plasma.【Result】Chlortetracycline concentration-time data fitted to a non-compartment model were analyzed by WinNonlin5.2.1 pharmacokinetic program after intravenous and oral administration in both fasted and fed pigs. The main pharmacokinetic parameters for chlortetracycline hydrochloride in fasted pigs and feeding pigs were follows: AUC0-∞ (57.42±23.53) mg·h·mL-1, (37.58±21.30) mg·h·mL-1; V/F (5.67±2.12) L·kg-1, (12.59±6.43) L·kg-1; MRT(13.87±2.00) h, (22.17±14.47) h; t1/2(19.93±5.26 ) h, (27.79±12.82) h, respectively. After receiving 10% chlortetracycline microspheres and 15% chlortetracycline microspheres without feeding, the parameters were as follows: AUC0-∞ (34.46±10.28) mg·h·mL-1, (33.15±12.76) mg·h·mL-1; Cmax (2.48±1.05) mg·mL-1, (2.97±1.88) mg·mL-1, Tmax (4.88±1.25) h, (3.13±1.55) h; V/F (31.53±15.98) L·kg-1, (32.30±9.69) L·kg-1; MRT (19.93±3.83) h, (17.41±1.80) h; t1/2(16.87±3.49) h, (17.13±3.58) h; F(17.03±0.08) %, (15.82±5.16) %, respectively. In feeding group, after receiving 10% chlortetracycline microspheres and 15% chlortetracycline microspheres, the parameters were as follows: AUC0-∞ (20.81±7.46) mg·h·mL-1, (19.72±5.69) mg·h·mL-1; Cmax (1.02±0.38) mg·mL-1, (0.95±0.32) mg·mL-1; Tmax (6.38±4.44) h, (8.00±5.24) h; V/F (52.40±22.90) L·kg-1,(52.47±19.69) L·kg-1; MRT (24.67±9.52) h, (23.37±4.21) h; t1/2 (18.57±10.67) h, (16.64±5.12) h; F (16.07±6.78)%, (15.26±5.26)%, respectively. 【Conclusion】The results of studies showed that chlortetracycline was absorbed slowly, distributed widely and had slow elimination and low bioavailability in pigs after receiving chlortetracycline microspheres orally. Compared with the fasting group, 10% and 15% chlortetracycline microspheres in the feeding group reached a slower peak time and a lower peak concentration, while the apparent distribution volume was larger and the bioavailability was lower, but the difference was not significant. This result indicated the feed did not affect the absorption of chlortetracycline in the gastrointestinal tract of pigs, but changed the pharmacokinetic process of chlortetracycline entering the body.

    Transcriptomic Analysis of Genes Related to Nursing Behavior in the Brains of Apis mellifera ligustica
    GAO Yan,ZHU YaNan,LI QiuFang,SU SongKun,NIE HongYi
    Scientia Agricultura Sinica. 2020, 53(19):  4092-4102.  doi:10.3864/j.issn.0578-1752.2020.19.021
    Abstract ( 299 )   HTML ( 16 )   PDF (982KB) ( 225 )   Save
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    【Objective】The nursing behavior of Apis mellifera ligustica plays an important role in maintaining colony stability and production of royal jelly (RJ). In this study, samples (3-day-old worker bees, 10-day-old nurses/foragers, 21-day-old nurses/foragers) were obtained by constructing artificial colony, which can eliminate the influence of age on nursing behavior. Moreover, genes closely related to the nursing behavior were strictly screened out in the brain of these samples, which could reveal the molecular network of brain regulation of nursing behavior.【Method】The 3-day-old worker bees, 10-day-old nurses and foragers, 21-day-old nurses and foragers were obtained by constructing artificial bee colony. And then the head of different group’s worker bees was dissected to obtain brain tissues of these samples. RNA-seq was used to analyze the transcriptome sequencing of the 5 groups (3-day-old worker bees, 10-day-old nurses, 10-day-old foragers, 21-day-old nurses, 21-day-old foragers) of brain samples. Differentially expressed genes (DEGs) which are closely related to the nursing behavior in the nurses’ brain were screened out. GO and KEGG enrichment analysis were carried out for these genes. qPCR was used to verify the expression patterns of 4 randomly selected DEGs.【Result】RNA-seq analysis screened out 32 DEGs that were closely related to the nursing behavior of nurses. These genes were significantly up-regulated in the brain of 10-day-old nurses than 3-day-old worker bees, 10-day-old foragers and 21-day-old foragers, and the expression level in the brain of 21-day-old nurses was significantly higher than that in 21-day-old foragers. GO enrichment analysis showed that the up-regulated DEGs were mainly involved in oxidoreductase activity, odor binding, transmembrane transport and other functional items. KEGG enrichment results showed that the up-regulated DEGs were mainly involved in protein metabolism (ribosome), energy metabolism (oxidative phosphorylation, carbon metabolism, the citrate cycle (TCA cycle), starch and sucrose metabolism, nitrogen metabolism and other glycan degradation pathway), signal transduction (Toll and Imd signaling pathways, phototransduction, sphingolipid metabolism), digestive function (lysosome). Of them, only sphingolipid metabolism and other glycan degradation pathway were notably enriched. qPCR results showed that the expression patterns of 3 up-regulated DEGs (LOC409709, LOC551813, LOC409708) and 1 down-regulated DEG (LOC551165) were consistent with the sequencing data.【Conclusion】To obtain gene expression profiles of brains from age-matched nurses and foragers, the brains of 5 groups (3-day-old worker bees, 10-day-old nurses, 10-day-old foragers, 21-day-old nurses, and 21-day-old foragers) were comprehensively analyzed using transcriptome sequencing, revealing that the 32 up-regulated DEGs were associated with brain nursing behavior. These genes were mainly involved in signal transduction and energy metabolism, which can affect the nursing behavior.