Abstract:

【Objective】 Antioxidant activity of bone protein hydrolysates and mode of antioxidative action were studied in this paper. 【Method】Bone protein was hydrolyzed for 0.5-6 h by alcalase. The mode of antioxidative action was evaluated by reducing power, metal-chelation ability and radical-scavenging. 【Result】 Bone protein hydrolysates possessed strong Cu2+- and Fe2+-chelation ability and marked reducing power, both of which were increased with hydrolysis time. The protein hydrolysates also showed strong radical-scavenging ability, and the antioxidant activity increased with substrate concentration. The antioxidant activity of 7% alcalase-hydrolyzed bone protein was comparable to those of butylated hydroxyanisole and proply gallate, and its antioxidant activity was close to proply gallate. Although intact bone protein displayed an antioxidative effect, it was far less potent than hydrolyzed bone protein. 【Conclusion】 The results demonstrated that enzyme-hydrolyzed bone protein can act as a metal ion chelator, a hydrogen donor, as well as a radical stabilizer to inhibit lipid oxidation.

Key words: bone protein, hydrolysis, antioxidant activity, lipid oxidation