Scientia Agricultura Sinica ›› 2024, Vol. 57 ›› Issue (15): 3071-3082.doi: 10.3864/j.issn.0578-1752.2024.15.012

• FOOD SCIENCE AND ENGINEERING • Previous Articles     Next Articles

Effects of the Different Tomato Addition Ratios on the Eating Quality and Oxidation of Stewed Beef

YOU Min1(), CHEN JinSong1(), CHEN YanYan1, YANG Ping1, ZHANG ChunHui1,2, HUANG Feng1,2()   

  1. 1 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Beijing 100193
    2 Institute of Western Agriculture, Chinese Academy of Agricultural Sciences, Changji 831100, Xinjiang
  • Received:2024-01-05 Accepted:2024-04-15 Online:2024-08-05 Published:2024-08-05
  • Contact: HUANG Feng

Abstract:

Objective】This study aimed to clarify the effects of adding tomatoes on the quality characteristics, lipid and protein oxidation inhibition of stewed beef, so as to provide insights into the interaction mechanism between tomato and beef components, thereby contributing to the development of a standardized production process.【Method】Tomato was added to the stewed beef in proportions of 0, 50%, 100%, 150%, and 200%, respectively, and which were stewed for 20 min. The quality characteristics of stewed beef, including pH value, color, and tenderness, were measured. The content of amino acids, nucleotides, and the taste of stewed beef brisket with tomato were determined and evaluated. Besides, the degrees of lipid and protein oxidation in beef were studied as well. 【Result】With higher ratios of added tomato, the pH value and L* value of beef significantly decreased (P<0.05), while the a* value increased. On the other hand, the shear force of beef significantly decreased and the myofibrillar fragmentation index (MFI) increased (P<0.05), which positively affected the color and tenderness of beef. Higher content of aspartic acid, glutamic acid, and proline in the soup and higher content of free amino acids such as glutamic acid and histidine in beef were observed as tomato added, while the content of 5′-AMP, 5′-GMP and 5′-IMP in beef were decreased (P<0.05). The addition of tomato resulted in a reduction in the levels of beef Schiff base (SB), TBARS, and the total sulfhydryl group. However, it increased the free sulfhydryl content. There was no significant effect on the carbonyl content. It could be assumed that tomatoes could inhibit the lipid oxidation and protein oxidation of stewed beef. The tomato addition ratios of 100%, 150%, and 200% had no significant effect on sensory acceptability. However, combining the content of free amino acids, nucleotides and the lipid and protein oxidation of beef, it was found that adding 200% tomato during beef stew resulted in better edible quality and greater oxidation inhibition ability. 【Conclusion】The addition of tomato stew could increase the redness, tenderness, and free amino acid content, and accelerate the release nucleotides of beef. Addition of tomatoes could inhibit the lipids oxidation and proteins oxidation of beef. Overall, addition of tomatoes results in beef with good overall acceptability and suppress oxidation.

Key words: tomato, beef, edible quality, taste, lipid oxidation, protein oxidation

Table 1

Effects of different tomato addition ratios on sensory evaluation of beef"

感官评定
Sensory analysis
处理 Treatment
0 50 100 150 200
色泽 Color 6.75±0.76a 6.65±0.59a 6.87±0.39a 6.60±0.70a 6.81±0.27a
滋味Taste 5.40±0.90b 3.67±0.92c 6.24±0.72ab 6.63±0.97a 6.4±0.55a
气味Flavor 6.24±0.79b 6.55±0.62ab 7.03±0.67a 6.97±0.71a 6.69±0.47ab
质地Texture 5.85±0.96a 6.15±0.92a 6.45±0.92a 6.41±0.94a 6.52±0.90a
总体可接受度 Overall-acceptability 5.96±0.47ab 5.73±0.63b 6.55±0.56a 6.51±0.73a 6.57±0.34a

Fig. 1

Effect of different tomato addition ratios on the pH values of beef Different letters indicate that the values are significantly different (P<0.05). The same as below"

Table 2

Effects of different tomato addition ratios on color of beef"

处理
Treatment
参数 Parameter
L* a* b*
0 52.57±1.62a 2.80±0.54b 13.61±0.83a
50% 52.34±1.77a 3.23±0.83ab 13.52±0.78a
100% 46.83±2.48b 3.66±0.57a 12.83±0.67a
150% 48.52±1.31b 3.69±0.15a 13.38±0.38a
200% 47.20±2.85b 3.79±0.39a 12.91±0.59a

Fig. 2

Effects of different tomato addition ratios on the cooking loss of beef"

Fig. 3

Effects of different tomato addition ratios on the shear force of beef"

Fig. 4

Effects of different tomato addition ratios on MFI in beef"

Table 3

Effect of different tomato addition on free amino acid contents of beef"

氨基酸名称
Amino acid name
肉样Beef 汤汁Soup
0 50% 100% 150% 200% 0 50% 100% 150% 200%
天冬氨酸 Aspartic acid - - - - - 5.11±0.16c 1.33±0.95d 2.18±0.76d 11.85±0.02b 16.82±5.02a
谷氨酸 Glutamate 3.54±0.07b 4.55±0.52b 8.59±1.59a 8.59±0.08a 9.70±0.29a 5.17±0.05a 6.91±0.05a 6.99±3.27a 15.10±4.59a 11.35±4.07a
鲜味氨基酸 Umami amino acid 3.54±0.07 4.55±0.52 8.59±1.59 8.59±0.08 9.70±0.29 10.28±0.21 8.24±1.00 9.16±4.03 26.95±4.61 28.17±9.09
丝氨酸 Serine 3.81±0.02b 7.78±1.42a 3.91±0.68b 4.33±1.38ab 3.62±0.75b 5.17±0.05c 12.41±1.65b 17.60±1.02a 10.52±1.00b 4.48±2.08c
甘氨酸 Glycine 2.86±0.16a 2.86±0.71a 3.88±1.32a 4.35±0.78a 2.73±0.01a 5.88±0.05b 2.25±0.62c 0.52±0.44c 1.28±0.24c 12.55±0.69a
苏氨酸 Threonine 7.30±0.29b 8.93±0.09a 7.15±0.88b 6.73±0.14b 7.73±0.31ab 8.50±1.24b 4.27±1.93b 0.56±1.90b 1.38±0.33b 19.23±3.53a
丙氨酸 Alanine 1.40±0.04b 13.50±1.58a 1.22±0.04b 1.25±0.01b 4.56±3.34b 5.22±3.14b 10.10±1.33b 5.14±1.20b 4.20±0.51b 17.20±1.04a
脯氨酸 Proline 1.28±0.08a 2.29±0.46a 3.68±1.36a 2.94±0.31a 2.53±1.26a 4.17±2.38b 5.53±0.28b 6.23±1.71b 6.03±0.56b 12.87±1.04a
甜味氨基酸 Sweet amino acid 16.65±0.59 35.36±4.26 19.84±4.28 19.59±2.62 21.17±5.67 28.94±6.86 34.56±5.81 30.05±6.27 23.41±2.64 66.33±8.38
组氨酸 Histidine 7.74±0.14b 17.23±3.84a 18.95±1.65a 17.27±1.31a 18.90±0.83a 30.14±0.05a 62.74±13.15a 45.64±18.03a 49.29±9.15a 39.90±0.05a
精氨酸 Arginine 75.56±0.34b 92.95±2.44a 70.26±8.64b 65.15±0.81b 77.61±2.51b 5.63±0.05c 126.80±22.76a 71.05±27.45ab 53.63±4.67bc 55.38±1.52bc
酪氨酸 Tyrosine 4.05±0.04ab 4.08±0.05ab 4.06±0.01ab 4.03±0.01b 4.24±0.12a 4.93±3.72a 1.57±0.30a 1.15±0.15a 3.99±3.11a 7.27±0.19a
缬氨酸 Valine 3.00±0.20a 3.23±0.01a 3.17±0.09a 3.03±0.02a 3.18±0.03a 4.24±2.86a 1.77±0.24a 1.14±0.31a 0.88±0.31a 2.65±1.53a
蛋氨酸 Methionine - - - - - - - - - -
赖氨酸 Lysine 4.00±0.55a 4.21±0.87a 4.23±0.65a 4.78±0.31a 5.34±0.15a 14.88±4.91a 12.71±1.17a 10.34±2.10a 7.93±0.08a 12.90±5.37a
异亮氨酸 Isoleucine 4.90±0.60a 4.21±0.11a 4.09±0.11a 3.99±0.01a 4.38±0.13a - - - - -
亮氨酸 Leucine 3.60±0.11c 4.04±0.06ab 3.88±0.06b 3.87±0.01b 4.12±0.11a 2.51±1.11a 4.22±0.05a 4.26±0.38a 3.74±0.08a 3.95±0.14a
苯丙氨酸 Phenylalanine 2.75±0.24a 2.77±0.25a 3.08±0.21a 2.93±0.05a 3.03±0.04a - - - - -
苦味氨基酸 Bitter amino acid 105.60±2.22 132.72±7.63 111.71±11.43 105.04±2.53 120.80±3.92 62.33±10.53 209.81±12.70 133.58±48.42 119.46±17.40 122.05±8.80
胱氨酸 Cystine 1.96±0.04a 2.09±0.04a 2.09±0.11a 2.02±0.01a 2.08±0.05a 5.28±0.05b 3.60±0.01b 3.52±0.01b 2.60±0.81b 3.02±0.21a
总氨基酸 Total free acids 127.75±2.92 174.72±12.45 142.24±17.40 135.26±5.24 153.75±9.93 106.83±19.82 256.21±44.49 176.32±58.73 172.42±25.46 219.57±26.48

Table 4

Effects of different tomato addition ratios on nucleotides contents of beef"

核苷酸名称
Nucleotide name
处理 Treatment
0 50% 100% 150% 200%
5′-AMP 8.28±0.78a 8.12±1.23ab 6.46±1.37ab 5.52±0.36b 6.65±1.00ab
5′-GMP 3.43±0.25a 2.91±0.31b 2.59±0.71ab 2.18±0.20b 2.65±0.57ab
5′-IMP 19.63±1.50a 19.03±3.70a 16.45±2.98ab 11.27±0.88b 14.6±3.73ab

Fig. 5

Effects of different tomato addition ratios on TBARS values of beef"

Fig. 6

Effect of different tomato addition ratios on carbonyl content of beef"

Fig. 7

Effect of different tomato addition ratios on total sulfhydryl content of beef"

Fig. 8

Effects of different tomato addition ratios on free sulfhydryl content of beef"

Fig. 9

Effect of different tomato addition ratios on Schiff Bases of beef"

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doi: 10.7506/spkx1002-6630-20220804-045
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