黄腐酸对番茄幼苗适应低磷胁迫的生理调控作用

doi:10.3864/j.issn.0578-1752.2018.08.012

Abstract

Abstract: 【Objective】Phosphorus is one of the key elements of plant growth and development. Phosphorus deficiency can seriously affect crop yield and quality. Fulvic acid, as a natural organic compound, can promote plant growth and improve plant stress resistance. In order to ascertain the physiological regulatory mechanisms of fulvic acid on stress tolerance of tomato seedlings against phosphate deficiency, we will execute this experiment to explain the mitigative mechanisms of “genetic phosphate deficiency” by fulvic acid in tomato seedlings. It has launched the experiment to study on physiological control of tomato seedlings adapting to low phosphorus stress by fulvic acid. 【Method】 Tomato cultivar of ‘JinPeng No.1’ was used as the tested cultivar, and Hogland nutrient solution hydroponics was used in this experiment. When the tomato seedlings grew to three leaves, they were moved to the hydroponic basin for seven days. We studied the roles of applying different concentrations of exogenous fulvic acid (0, 0.04, 0.08, 0.12, 0.16, and 0.20 g·L^-1; T0-T5 ) on the growth of tomato seedlings, root development, ability of photosynthesis, uptake and distribution of phosphate, accumulation and secretion of organic acid, and so on under hydroponic environment of phosphate deficiency.【Result】With the increase of the amount of fulvic acid (T1-T5), the physiological indexes of tomato seedlings showed a trend of rising first and then decreasing under low-phosphorus stress. Application of fulvic acid to 0.08 g·L^-1 had a significant effect on increasing ratio of root to shoot; improving chlorophyll content, photosynthetic performance of leaves, enhancing phosphorus accumulation, distribution and transport in different tissues of tomato，phosphorus transporter-related gene's expression (PT1 and PHO1). PT1 can promote the roots of tomato seedlings to the environment in the uptake of phosphate alleviate low phosphorus stress and PHO1 can promote phosphate from root to stem and leaf distribution in order to effectively alleviate the shoot phosphorus deficiency. It can also improve the accumulation and secretion of organic acid (oxalate, malate, citrate, succinate, and tartrate), reducing the roots of anaerobic respiration products of lactic acid and acetic acid, promoting the proton pump gene (HA1) expression that can effectively change the environment of insoluble phosphorus to soluble phosphate and increased soluble phosphate content in the environment to promote plant root phosphorus content, improving growth- and development-related gene's expression (GRAS1) as well as decreasing the anthocyanin accumulation. 【Conclusion】Adding fulvic acid at a certain concentration under low-phosphorus stress can significantly improve shoot growth and root development of tomato seedlings. It indicates that the addition of fulvic acid can alleviate the phosphorus deficiency symptoms of plants to a certain extent and reveal the regulation of fulvic acid physiological mechanism of tomato actively against phosphorus deficiency.

Key words: tomato, fulvic acid, phosphate deficiency, physiological regulation

ZHANG LiLi, LIU DeXing, SHI QingHua, GONG Biao. Physiological Regulatory Effects of Fulvic Acid on Stress Tolerance of Tomato Seedlings Against Phosphate Deficiency[J].Scientia Agricultura Sinica, 2018, 51(8): 1547-1555.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2018.08.012

https://www.chinaagrisci.com/EN/Y2018/V51/I8/1547

References

[1] 展晓莹, 任意, 张淑香, 康日峰. 中国主要土壤有效磷演变及其与磷平衡的响应关系. 中国农业科学, 2015, 48(23): 4728-4737.

ZHAN X Y, REN Y, ZHANG S X, KANG R F. Changes in olsen phosphorus concentration and its response to phosphorus balance in the main types of soil in China. Scientia Agricultura Sinica, 2015, 48(23): 4728-4737. (in Chinese)

[2] 康利允, 沈玉芳, 岳善超, 李世清. 不同水分条件下分层施磷对冬小麦根系分布及产量的影响. 农业工程学报, 2014, 30(15): 140-147.

KANG L Y, SHEN Y F, YUE S C, LI S Q. Effect of phosphorus application in different soil depths on root distribution and grain yield of winter wheat under different water conditions. Transactions of the Chinese Society of Agricultural Engineering, 2014, 30(15): 140-147. (in Chinese)

[3] 李粉茹, 于群英, 邹长明. 设施菜地土壤pH值、酶活性和氮磷养分含量的变化. 农业工程学报, 2009, 25(1): 217-222.

LI F R, YU Q Y, ZOU C M. Variations of pH value, enzyme activity and nitrogen phosphorus content in protected vegetable soils. Transactions of the Chinese Society of Agricultural Engineering, 2009, 25(1): 217-222. (in Chinese)

[4] 张璇, 郝芳华, 王晓, 王云慧, 欧阳威. 河套灌区不同耕作方式下土壤磷素的流失评价. 农业工程学报, 2011, 27(6): 59-65.

ZHANG X, HAO F H, WANG X, WANG Y H, OU Y W. Assessment of phosphorus loss under different tillage methods in Hetao agricultural irrigation areas. Transactions of the Chinese Society of Agricultural Engineering, 2011, 27(6): 59-65. (in Chinese)

[5] 刘秀梅, 张夫道, 冯兆滨, 张树清, 何绪生, 王茹芳, 王玉军. 风化煤腐殖酸对氮、磷、钾的吸附和解吸特性. 植物营养与肥料学报, 2005, 11(5): 641-646.

LIU X M, ZHANG F D, FENG Z B, ZHANG S Q, HE X S, WANG R F, WANG Y J. N , P and K adsorption and desorption characteristics of humic acids made from the airslake-coal. Plant Nutrition and Fertilizer Science, 2005, 11(5): 641-646. (in Chinese)

[6] 韩玉国, 任树梅, 李云开, 张文理, 刘洪禄. 黄腐酸(FA)旱地龙在苹果节水生产中的应用效果研究. 农业工程学报, 2004, 20(6): 93-97.

HAN Y G, REN S M, LI Y K, ZHANG W L, LIU H L. Experimental study on effect of FA Handilong on water saving and production of apple tree in the rural areas in Beijing. Transactions of the Chinese Society of Agricultural Engineering, 2004, 20(6): 93-97. (in Chinese)

[7] CANELLAS L P, OLIVARES F L, AGUIAR N O,JONES D L, NEBBIOSO A, MAZZEI P, PICCOLO A. Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulture, 2015, 196: 15-27.

[8] 李春越, 党廷辉, 王万忠, 刘文兆. 腐殖酸对农田土壤磷素吸附行为的影响研究. 水土保持学报, 2011, 25(3): 77-82.

LI C Y, DANG T H, WANG W Z, LIU W Z. Influence of humic acid on the adsorption behavior of phosphorus in agricultural soil.Journal of Soil and Water Conservation, 2011, 25(3): 77-82. (in Chinese)

[9] HOAGLAND D R, ARNON D I. The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular, 1950, 347: 1-32.

[10] GONG B, Li X, VANDENLANGENBERG K M, WEN D, SUN S S, WEI M, LI Y, YANG F J, SHI Q H, WANG X F. Overexpression of S-adenosyl-L-methionine synthetase increased tomato tolerance to alkali stress through polyamine metabolism. Plant Biotechnology Journal, 2014, 12(6): 694-708.

[11] GONG B, LI X, BLOSZIES S, WEN D, SUN S S, WEI M, LI Y, YANG F J, SHI Q H, WANG X F. Sodic alkaline stress mitigation by interaction of nitric oxide and polyamines involves antioxidants and physiological strategies in Solanum lycopersicum. Free Radical Biology and Medicine, 2014, 71(6): 36-48.

[12] GONG B, WEN D, BLOSZIES S, LI X, WEI M, YANG F J, SHI Q H, WANG X F. Comparative effects of NaCl and NaHCO₃ stresses on respiratory metabolism, antioxidant system, nutritional status, and organic acid metabolism in tomato roots. Acta Physiologiae Plantarum, 2014, 36(8): 2167-2181.

[13] BONNOT C, PROUST H, PINSON B, COLBALCHINI F P, LESLY- VEILLARD A, BREUNINGER H, CHAMPION C, HETHERINGTON A J, KELLY S, DOLAN L. Functional PTB phosphate transporters are present in streptophyte algae and early diverging land plants. New Phytologist, 2017, 214(3): 1158-1171.

[14] STEFANOVIC A, RIBOT C, ROUACHED H, WANG Y, CHONG J, BELBAHRI L, DELESSERT S, POIRIER Y. Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. The Plant Journal, 2007, 50(6): 982-994.

[15] LI L, LI S M, SUN J H, ZHOU L L, BAO X G, ZHANG H G, ZHANG F S. Diversity enhances agricultural productivity via rhizosphere phosphorus facilitation on phosphorus-deficient soils. Proceedings of the National Academy of Sciences, 2007, 104(27): 11192-11196.

[16] YAN F, ZHU Y, MÜLLE C, SCHUBERT S. Adaptation of plasma membrane H⁺-ATPase of proteoid roots of white lupin (Lupinus albus L.) to phosphorus deficiency. Developments in Plant and Soil Sciences, 2001, 92(3): 186-187.

[17] BATTAGLIA M, RÍPODAS C, CLÚA J, BAUDIN M, AGUILAR O M, NIEBEL A, ZANETTI M E, BLANCO F A. A nuclear factor Y interacting protein of the GRAS family is required for nodule organogenesis, infection thread progression, and lateral root growth. Plant Physiology, 2014, 164(3): 1430-1442.

[18] 唐晓乐, 李兆君, 马岩, 梁永超. 低温条件下黄腐酸和有机肥活化黑土磷素机制. 植物营养与肥料学报, 2012, 18(4): 893-899.

TANG X L, LI Z J, MA Y, LIANG Y C. Mechanism of fulvic acid-and organic manure-mediated phosphorus mobilization in black soil at low temperature. Plant Nutrition and Fertilizer Science, 2012, 18(4): 893-899. (in Chinese)

[19] 谷端银, 王秀峰, 杨凤娟, 焦娟, 魏珉, 史庆华. 纯化腐殖酸对低氮胁迫下黄瓜幼苗生长及养分吸收的影响. 应用生态学报, 2016, 27 (8): 2535-2542.

GU D Y, WANG X F, YANG F J, JIAO J, WEI M, SHI Q H. Effects of purified humic acid on growth and nutrient absorption of cucumber (Cucumis sativus) seedlings under low nitrogen stress.Chinese Journal of Applied Ecology, 2016, 27(8): 2535-2542. (in Chinese)

[20] 何军, 崔远来, 王建鹏, 史伟达. 不同尺度稻田氮磷排放规律试验. 农业工程学报, 2010, 26(10): 56-62.

HE J, CUI Y L, WANG J P, SHI W D. Experiments on nitrogen and phosphorus losses from paddy fields under different scales. Transactions of the Chinese Society of Agricultural Engineering, 2010, 26(10): 56-62. (in Chinese)

[21] 刘方春, 邢尚军, 刘春生, 杜振宇, 段春华. 不同处理褐煤腐殖酸对磷吸附特性的研究. 水土保持学报, 2008, 22(2): 177-181.

LIU F C, XING S J, LIU C S, DU Z Y, DUAN C H. Characteristics of adsorption of P on humic acid extracted from different treated brown coal. Journal of Soil and Water Conservation, 2008, 22(2): 177-181. (in Chinese)

[22] ROUACHED H, ARPAT. , 2010, 3(2): 288-299. A B , POIRIER Y. Regulation of phosphate starvation responses in plants: signaling players and cross-talksMolecular Plant

[23] 陈屏昭, 王磊, 代勋, 刘忠荣, 蒋彬, 樊钦平. 缺磷强光下脐橙的

过剩能量耗散机制. 应用生态学报, 2005, 16(6): 1061-1066.

CHEN P Z, WANG L, DAI X, LIU Z R, JIANG B, FAN Q P. Dissipation mechanism of excessive energy in umbilical orange under phosphorus deficiency and strong light stress. Chinese Journal of Applied Ecology, 2005, 16(6): 1061-1066. (in Chinese)

[24] 兰忠明, 林新坚, 张伟光, 张辉, 吴一群. 缺磷对紫云英根系分泌物产生及难溶性磷活化的影响. 中国农业科学, 2012, 45(8): 1521-1531.

LAN Z M, LIN X J, ZHANG W G, ZHANG H, WU Y Q. Effect of P deficiency on the emergence of Astragalus L. root exudates and mobilization of sparingly soluble phosphorus. China Agriculture Science, 2012, 45(8): 1521-1531. (in Chinese)

[25] 梅慧生, 杨玉明, 张淑运, 曹宗巽. 腐殖酸钠对植物生长的刺激作用. 植物生理学报, 1980, 6(2): 133-140.

MEI H S, YANG Y M, ZHANG S Y, CAO Z X. The stimulation effect of an humate on plant growth. Plant Physiology Journal, 1980, 6(2): 133-140. (in Chinese)

[26] 周霞萍, 邓林, 韩媛媛, JOHN P C, TIANRUI S, 黄琛. 腐殖酸天然植物生长素调节剂. 中国生物防治, 2009, 22(s1): 38-38.

ZHOU X P, DENG L, HAN Y Y, JOHN P C, TIANRUI S, HUANG C. Humic acid, natural plant growth regulator. Chinese Journal of Biological Control, 2009, 22(s1): 38-38. (in Chinese)

[27] SÁNCHEZ C, VIELBA J M, FERRO E, COVELO G, SOLÉ A, ABARCA D, DE M B, DÍAZ-SALA C. Two SCARECROW-LIKE genes are induced in response to exogenous auxin in rooting- competent cuttings of distantly related forest species. Tree Physiology, 2007, 27(10): 1459-1470.

[28] HEO J O, CHANG K S, KIM I A, LEE S A, SONG S K, LEE M M, LIM J. Funneling of gibberellin signaling by the GRAS transcription regulator scarecrow-like 3 in the Arabidopsis root. Proceedings of the National Academy of Sciences, 2011, 108(5): 2166-2171.

[29] JIANG C, GAO X, LIAO L, HARBERD N, FU X. Phosphate starvation root architecture and anthocyanin accumulation responses are modulated by the gibberellin-DELLA signaling pathway in Arabidopsis. Plant Physiology, 2007, 145(4): 1460-1470.

[30] RIBOT C, WANG Y, POIRIER Y. Expression analyses of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the responses to auxin, cytokinin, and abscisic acid. Planta, 2008, 227(5): 1025-1036.

Related Articles 15

[1]	SHAO ShuJun,HU ZhangJian,SHI Kai. The Role and Mechanism of Linoleyl Ethanolamide in Plant Resistance Against Botrytis cinerea in Tomato [J]. Scientia Agricultura Sinica, 2022, 55(9): 1781-1789.
[2]	WANG MengRui, LIU ShuMei, HOU LiXia, WANG ShiHui, LÜ HongJun, SU XiaoMei. Development of Artificial Inoculation Methodology for Evaluation of Resistance to Fusarium Crown and Root Rot and Screening of Resistance Sources in Tomato [J]. Scientia Agricultura Sinica, 2022, 55(4): 707-718.
[3]	HU XueHua,LIU NingNing,TAO HuiMin,PENG KeJia,XIA Xiaojian,HU WenHai. Effects of Chilling on Chlorophyll Fluorescence Imaging Characteristics of Leaves with Different Leaf Ages in Tomato Seedlings [J]. Scientia Agricultura Sinica, 2022, 55(24): 4969-4980.
[4]	LIU Hao,PANG Jie,LI HuanHuan,QIANG XiaoMan,ZHANG YingYing,SONG JiaWen. Effects of Foliar-Spraying Selenium Coupled with Soil Moisture on the Yield and Quality of Tomato [J]. Scientia Agricultura Sinica, 2022, 55(22): 4433-4444.
[5]	CUI QingQing, MENG XianMin, DUAN YunDan, ZHUANG TuanJie, DONG ChunJuan, GAO LiHong, SHANG QingMao. Inhibiting Eeffect of Root-Cutting and Top-Pinching on Graft Healing of Tomato [J]. Scientia Agricultura Sinica, 2022, 55(2): 365-377.
[6]	LI YiMei,WANG Jiao,WANG Ping,SHI Kai. Function of Sugar Transport Protein SlSTP2 in Tomato Defense Against Bacterial Leaf Spot [J]. Scientia Agricultura Sinica, 2022, 55(16): 3144-3154.
[7]	FANG HanMo,HU ZhangJian,MA QiaoMei,DING ShuTing,WANG Ping,WANG AnRan,SHI Kai. Function of SlβCA3 in Plant Defense Against Pseudomonas syringae pv. tomato DC3000 [J]. Scientia Agricultura Sinica, 2022, 55(14): 2740-2751.
[8]	LI JianXin,WANG WenPing,HU ZhangJian,SHI Kai. Effects of Simulated Acid Rain Conditions on Plant Photosynthesis and Disease Susceptibility in Tomato and Its Alleviation of Brassinosteroid [J]. Scientia Agricultura Sinica, 2021, 54(8): 1728-1738.
[9]	XianMin MENG,YanHai JI,WangWang SUN,ZhanHui WU,ZhaoSheng CHU,MingChi LIU. Response of Chloroplast Ultrastructure and Photosynthetic Physiology of Two Tomato Varieties to Low Light Stress [J]. Scientia Agricultura Sinica, 2021, 54(5): 1017-1028.
[10]	WANG Ping,ZHENG ChenFei,WANG Jiao,HU ZhangJian,SHAO ShuJun,SHI Kai. The Role and Mechanism of Tomato SlNAC29 Transcription Factor in Regulating Plant Senescence [J]. Scientia Agricultura Sinica, 2021, 54(24): 5266-5276.
[11]	ZHANG JiFeng,WANG ZhenHua,ZHANG JinZhu,DOU YunQing,HOU YuSheng. The Influences of Different Nitrogen and Salt Levels Interactions on Fluorescence Characteristics, Yield and Quality of Processed Tomato Under Drip Irrigation [J]. Scientia Agricultura Sinica, 2020, 53(5): 990-1003.
[12]	LI YueYue,ZHOU WenPeng,LU SiQian,CHEN DeRong,DAI JianHong,GUO QiaoYou,LIU Yong,LI Fan,TAN GuanLin. Occurrence and Biological Characteristics of Tomato mottle mosaic virus on Solanaceae Crops in China [J]. Scientia Agricultura Sinica, 2020, 53(3): 539-550.
[13]	DU Xia,WU Kuo,LIU Xia,ZHANG LiZhen,SU XiaoXia,ZHANG HongRui,ZHANG ZhongKai,HU XianQi,DONG JiaHong,YANG YanLi,GAO YuLin. The Occurrence Trends of Dominant Species of Potato Viruses and Thrips in Yunnan Province [J]. Scientia Agricultura Sinica, 2020, 53(3): 551-562.
[14]	ZOU LinFeng,TU LiQin,SHEN JianGuo,DU ZhenGuo,CAI Wei,JI YingHua,GAO FangLuan. The Evolutionary Dynamics and Adaptive Evolution of Tomato Chlorosis Virus [J]. Scientia Agricultura Sinica, 2020, 53(23): 4791-4801.
[15]	ZHANG LiLi,SHI QingHua,GONG Biao. Application of Fulvic Acid and Phosphorus Fertilizer on Tomato Growth, Development, and Phosphorus Utilization in Neutral and Alkaline Soil [J]. Scientia Agricultura Sinica, 2020, 53(17): 3567-3575.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Physiological Regulatory Effects of Fulvic Acid on Stress Tolerance of Tomato Seedlings Against Phosphate Deficiency

RichHTML

PDF