Scientia Agricultura Sinica ›› 2022, Vol. 55 ›› Issue (24): 4969-4980.doi: 10.3864/j.issn.0578-1752.2022.24.014

• RESEARCH NOTES • Previous Articles    

Effects of Chilling on Chlorophyll Fluorescence Imaging Characteristics of Leaves with Different Leaf Ages in Tomato Seedlings

HU XueHua1(),LIU NingNing1,TAO HuiMin1,PENG KeJia1,XIA Xiaojian2,HU WenHai1()   

  1. 1School of Life Sciences, Jinggangshan University, Ji’an 343009, Jiangxi
    2College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058
  • Received:2022-04-13 Accepted:2022-06-16 Online:2022-12-16 Published:2023-01-04
  • Contact: WenHai HU;


【Objective】This study analyzed the characteristics of chlorophyll fluorescence imaging of the 2nd (mature) and 4th (newly born) leaves of tomato seedlings in response to chilling stress, aiming to probe the mechanism of leaves with different ages adapted to chilling stress in tomato seedlings. 【Method】In this study, Solanum lycopersicum L. cv Zhongshu No. 4 was used as the research material. The tomato seedlings in the 4-leaf stage were treated at chilling (8℃, 200 μmol·m-2·s-1) for 15 d and then recovered at normal temperature (26℃ day/20℃ night, 500 μmol·m-2·s-1) for 1 d. The Chlorophyll fluorescence imagings of the whole seedlings were measured at different stages under different treatments, and the characteristics of chlorophyll fluorescence imaging of the 2nd and 4th leaves were compared. 【Result】The results showed that the relative area of photosynthetically active regions (RAP) on the 4th leaves decreased slowly during the first 5 d of the chilling stress, while the RAP on the 2nd leaves and the whole plants decreased steadily during the whole chilling treatment. The RAP recovered completely after 1 d of recovery. Under chilling treatment, the relative area of fluorescence active regions (RAF) for quantum yield of regulatory energy dissipation (Y(NPQ)), quantum yield of nonregulatory energy dissipation (Y(NO)), and nonphotochemical quenching (NPQ) showed similar changes as that of RAP. However, the RAF of effective PSII quantum yield (Y(II)) and coefficient of photochemical quenching (qP) were significantly lower than that of RAP. The maximum PSII quantum yield (Fv/Fm), Y(NPQ), and NPQ decreased while Y(NO) increased sharply in the photosynthetically active region in tomato seedlings during the first 5 d of the chilling treatment. In the following days, the Fv/Fm remained unchanged, whereas Y(NPQ) and NPQ increased and Y(NO) decreased. However, Y(II) declined sharply 1 d after chilling stress and then remained unchanged. Interestingly, qP in the photosynthetic active region of the 4th leaves decreased only slightly after 1 d of chilling treatment, and then maintained higher than that before treatment. However, qP of the 2nd leaves increased significantly on the 5th day and then decreased rapidly. Overall, the Fv/Fm, Y(II), Y(NPQ) and qP in the 4th leaves were higher than those in the 2nd leaves, whereas the Y(NO) were relatively lower in the 4th leaves. 【Conclusion】The study found that tomato seedlings responded to chilling stress by decreasing the area of photosynthetically active regions. The regulatory nonphotochemical quenching, which played a central role in photoprotection, was inhibited in the early stage, but gradually increased during the later stage under chilling treatment. The mature leaves adapted to chilling stress likely by decreasing the size of photosynthetically active regions, while higher capacities of PSII photochemistry and thermal dissipation were maintained in the newly born leaves in response to chilling stress. The protection of shoot apex and newly born leaves might be the priority of tomato seedlings in response to chilling stress. For newly born leaves, the chilling induced the closure of some active PSII reaction centers, but improved the operating efficiency of the remaining active PSII reaction centers, which was potentially beneficial for the recovery of photosynthetic activity.

Key words: tomato (Solanum lycopersicum L.), chilling stress, chlorophyll fluorescence imaging, photoinhibition, leaf age

Fig. 1

AOI selection (A) and photosynthetically active regions (B) of the 2nd and 4th leaves of tomato seedlings after chill-treated 5 d (taking plant 1 as an example)"

Fig. 2

Fluorescence imaging of Fv/Fm of tomato seedlings during chilling 15 d and subsequent recovering 1 d (taking plant 1 as an example)"

Fig. 3

Effects of chilling on relative area (RAP) and Fv/Fm of photosynthetically active regions in tomato seedlings"

Fig. 4

Fluorescence imaging of Y(II), Y(NPQ) and Y(NO) of tomato seedlings during chilling 15 d and subsequent recovering 1 d (taking plant 1 as an example)"

Fig. 5

Effects of chilling on Y(II), Y(NPQ) and Y(NO) of photosynthetically active regions in tomato seedlings"

Fig. 6

Fluorescence imaging of NPQ and qP of tomato seedlings during chilling 15 d and subsequent recovering 1 d (taking plant 1 as an example)"

Fig. 7

Effects of chilling on NPQ and qP of photosynthetically active regions in tomato seedlings"

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