Scientia Agricultura Sinica ›› 2016, Vol. 49 ›› Issue (15): 2921-2931.doi: 10.3864/j.issn.0578-1752.2016.15.006

• TILLAGE & CULTIVATION·PHYSIOLOGY & BIOCHEMISTRY·AGRICULTURE INFORMATION TECHNOLOGY • Previous Articles     Next Articles

Effects of DTA-6 and S3307 on Physiological Regulation in Normal and Abscission Pods of Soybean

CUI Hong-qiu1,2, FENG Nai-jie1, SUN Fu-dong1, LIU Chun-juan1, HE Tian-ming1, ZHAO Jing-jing1, LIU Yang1, GONG Shen1, SHI Chen2, ZHENG Dian-feng1   

  1. 1Agronomy College of Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang
    2Heilongjiang Academy of Agricultural Science Daqing Branch, Daqing 163316, Heilongjiang
  • Received:2016-03-07 Online:2016-08-01 Published:2016-08-01

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Abstract: 【Objective】The objective of this experiment is to study the effects of plant growth promoter and plant growth retardator on the difference of physiology in normal and abscission pods of soybean during the pods development in the semi-arid area, to discuss the physiological effects of plant growth regulator on reduction of the abscission of flowers and pods in soybean, and try to find the ways to increase soybean output. 【Method】 Diethyl aminoethyl hexanoate（DTA-6）, Uniconazole（S3307）and water were foliage sprayed at initial flowering (R1) stage of 3 varieties including Suinong 28 (SN28), Kenfeng 16 (KF16), and Hefeng 50 (HF50) in the Lindian county, Daqing city of Heilongjiang province in 2012 and 2013. The first time to take samples was 35 d (R5) after spraying, since then, the normal and abscission pods treated with CK and treatments were collected every seven days. The pod coats and seeds were separated, quick frozen for 30 min in the liquid nitrogen, and then stored in low temperature refrigerator(-40℃). Determination was carried out when all the samples were collected. The regulating differences of DTA-6 and S3307 between normal and abscission pods in oxygen free radical metabolism, related abscisic enzymes and soluble matter in soybean were determined and compared. 【Result】 The results demonstrated that during the pod development process, the malonaldehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, soluble sugar and soluble protein of abscission pods were significantly higher than normal pods, the abscission cellulose (AC) activity, polygalacturonase (PG) activity in abscission pods were significantly lower than normal pods. DTA-6 and S3307 could regulate the physiology of normal and abscission pods in soybean. Although DTA-6 and S3307 showed different process in regulating effect, but both in the process had many similar results. The regulating effects of DTA-6 and S3307 showed as follows: DTA-6 and S3307 treatments decreased MDA content, improved SOD, POD activities in normal pods, and decreased AC and PG activities at pod-filling early stage in SN28 and KF16, but decreased at pod-filling late stage in HF50. The physiological difference of normal and abscission pods could change with the pods developmental process in soybean. The extents of increase and decrease were different during pods development process. The physiological difference was influenced by genetically controlled factors.【Conclusion】Compared with normal pods, the physiological indexes of abscission pods were improved, plasma membrane peroxidation was enhanced, balance of protective enzyme system was broken, soluble material were increased, related abscission enzymes activities reduced, and these were affected by environmental factors. The normal pods regulated by DTA-6 and S3307 showed a positive response to biological membrane damage, osmotic adjustment, protective enzyme system, related abscission enzymes activity reduced, especially in improving the physiological regulation and self-repairing ability.

Key words: soybean, pods abscission, plant growth regulator, physiology, DTA-6, S3307

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