Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (24): 4769-4779.doi: 10.3864/j.issn.0578-1752.2014.24.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES •     Next Articles

Construction of Genetic Map Using Genotyping Chips and QTL Analysis of Grain Weight

CHEN Jian-sheng1, CHEN Guang-feng1, LI Qing-fang1, ZHANG Han2, SHI Cui-lan1, SUN Cai-ling1, DENG Zhi-ying1, LIU Kai1, GU Zhi-qun1 , TIAN Ji-chun1,3   

  1. 1Group of Wheat Quality Breeding, College of Agronomy, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong
    2Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100
  • Received:2014-05-05 Online:2014-12-16 Published:2014-12-16

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Abstract: 【Objective】Wheat genetic map is a very useful resource for genome analysis and understanding of the genetic basis of phenotypic variation. High density genetic maps were constructed by combining the traditonal molecular markers and modern gene microarray technology in order to carry out QTL analysis of grain weight, to detect the major QTL, and to supply a basis and direction of exploration of CAPS markers for molecular-assistant breeding, which could also lay a foundation for fine mapping and excavation of grain weight genes. 【Method】 A RIL (recombinant inbred lines) population including 173 lines was analyzed using wheat 90K SNP array，DArt technology and traditional moleculer technology. High density genetic map was constructed. QTL mapping for 1000- grain weight was analyzed using QTL network 2.0. 【Result】 A genetic map of wheat covering 21 wheat chrosmes was constructed and it contains 6 241 polymorphism markers, including 6 001 SNP，256 DArT，27 SSR, covering 4875.29 cM, with an average interval distance of 0.77 cM. A, B and D chromsomes possess 2 390, 3 386, and 468 polymorphism markers, accounting for the proportion of 38.3%, 54.3%, and 7.5%, separately. The average interval distances of the markers were 0.80, 0.75 and 0.80 cM, respectively. QTL analysis of 1000-grain weight was processed under four environments. Total 9 QTL controlling 1000-grain weight were detected located on 1B, 4B, 5B, and 6A. Additive effect of QGW4B-17, QGW4B-5, QGW4B-2, QGW6A-344 and QGW6A-137 was more than 10%, which were considered as major QTL. Among them, QGW4B-17 was detected under several environments, and the phenotypic variation explained by which varied from 16% to 33% with its additive effect from 2.3 to 2.97 g. All the additive effects come from big kernel parent Shannong 01-35，single QTL could increase 1.09-2.97g for 1000-grain weight. 【Conclusion】A genetic map of wheat covering 21 wheat chrosmes was constructed with 6 241 polymorphism markers, with an average interval distance of 0.77 cM. Total 9 QTL controlling 1000-grain weight located on 1B, 4B, 5B, and 6A were detected. Additive effect ofQGW4B-17 was detected under several environments, and the phenotypic variation explained by which varied at 16%-33% with its additive effect at 2.3-2.97 g.

Key words: common wheat, 90K array, QTL mapping, grain weight, SNP

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