Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (22): 4371-4379.doi: 10.3864/j.issn.0578-1752.2014.22.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES • Previous Articles     Next Articles

Study on Homozygous Progress Evaluation of Self-Pollination in Ramie (Boehmeria Nivea L.)

TAN Long-tao, YU Chun-ming, CHEN Ping, WANG Yan-zhou, CHEN Ji-kang, WEN Lan, ZHENG Jian-shu, XIONG He-ping   

  1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205
  • Received:2014-02-21 Revised:2014-07-04 Online:2014-11-16 Published:2014-11-16

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Abstract: 【Objective】The objective of this study was to demonstrate genetic diversity, population structure and the rate of purification of inbred progenies in ramie, and to further identify the homozygous trends. 【Method】A total of 31 pairs of SSR and 48 pairs of SRAP primers were screened out based on inbred progenies of Zhong Zhu No.1. Genetic diversity was analyzed by the homozygous locus ratio and genetic diversity parameters, population structure of inbred progenies were analyzed by using Structure, NTSYS- pcV2.1 and AMOVA softwares. Marker index(MI) was calculated to assess the discriminatory power of SSR and SRAP.【Result】 SSR and SRAP markers indicated that all genetic diversity parameters decreased obviously from S3 to S5, 57 and 157 polymorphic bands were amplified, the homozygous locus ratio increased by 5.2% and 4.61%, the average polymorphic loci dropped by 13 and 44, the average effective number of alleles dropped by 0.7883 and 2.1629, Nei’s gene diversity dropped by 0.1143 and 0.0684, and the average Shannon’s information index dropped by 0.0465 and 0.1207. The number of blue bands was over 65% in S5 generation groups by SSR and SRAP with Structure software. The dispersion of S3 generation group was higher than S4 and S5 generation groups, S3 generation groups mainly contain S4 and S5 generation groups. Population structure and PCA by SSR and SRAP showed the uniformity of inbred progenies increased gradually. Genetic variation among populations by SSR and SRAP was larger than the variation within populations with AMOVA software, and the total variation was more than 94%. The average heterozygosity obtained using SSR (Hav=0.4689) was higher than estimated using SRAP (Hav=0.4197). Mean effective multiplex ratio was maximum for the SRAP (EMR=3.2781) followed by SSR(EMR=1.8562). Consequently, the marker index, which is the mean of the product of Hav and EMR was maximum for SRAP followed by SSR.【Conclusion】SSR and SRAP are suitable for genetic diversity and populations structure of ramie germplasm, with the increase of the number of inbred generations, the uniformity of inbred progenies increased gradually, and the average heterozygosity(H) reduced gradually.

Key words: ramie (Boehmeria Nivea L.), SSR, SRAP, genetic diversity, population structure

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