苎麻自交纯合进度研究

doi:10.3864/j.issn.0578-1752.2014.22.003

Abstract

Abstract: 【Objective】The objective of this study was to demonstrate genetic diversity, population structure and the rate of purification of inbred progenies in ramie, and to further identify the homozygous trends. 【Method】A total of 31 pairs of SSR and 48 pairs of SRAP primers were screened out based on inbred progenies of Zhong Zhu No.1. Genetic diversity was analyzed by the homozygous locus ratio and genetic diversity parameters, population structure of inbred progenies were analyzed by using Structure, NTSYS- pcV2.1 and AMOVA softwares. Marker index(MI) was calculated to assess the discriminatory power of SSR and SRAP.【Result】 SSR and SRAP markers indicated that all genetic diversity parameters decreased obviously from S₃ to S₅, 57 and 157 polymorphic bands were amplified, the homozygous locus ratio increased by 5.2% and 4.61%, the average polymorphic loci dropped by 13 and 44, the average effective number of alleles dropped by 0.7883 and 2.1629, Nei’s gene diversity dropped by 0.1143 and 0.0684, and the average Shannon’s information index dropped by 0.0465 and 0.1207. The number of blue bands was over 65% in S₅ generation groups by SSR and SRAP with Structure software. The dispersion of S₃ generation group was higher than S₄ and S₅ generation groups, S₃ generation groups mainly contain S₄ and S₅ generation groups. Population structure and PCA by SSR and SRAP showed the uniformity of inbred progenies increased gradually. Genetic variation among populations by SSR and SRAP was larger than the variation within populations with AMOVA software, and the total variation was more than 94%. The average heterozygosity obtained using SSR (H_av=0.4689) was higher than estimated using SRAP (H_av=0.4197). Mean effective multiplex ratio was maximum for the SRAP (EMR=3.2781) followed by SSR(EMR=1.8562). Consequently, the marker index, which is the mean of the product of H_av and EMR was maximum for SRAP followed by SSR.【Conclusion】SSR and SRAP are suitable for genetic diversity and populations structure of ramie germplasm, with the increase of the number of inbred generations, the uniformity of inbred progenies increased gradually, and the average heterozygosity(H) reduced gradually.

Key words: ramie (Boehmeria Nivea L.), SSR, SRAP, genetic diversity, population structure

TAN Long-tao, YU Chun-ming, CHEN Ping, WANG Yan-zhou, CHEN Ji-kang, WEN Lan, ZHENG Jian-shu, XIONG He-ping. Study on Homozygous Progress Evaluation of Self-Pollination in Ramie (Boehmeria Nivea L.)[J].Scientia Agricultura Sinica, 2014, 47(22): 4371-4379.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2014.22.003

https://www.chinaagrisci.com/EN/Y2014/V47/I22/4371

References

[1] 李宗道. 麻作的理论与技术. 上海: 上海科学技术出版社, 1982: 187-193.

Li Z D. Theory and Technology System of Fiber Crops. Shanghai: Shanghai Scientific and Technical Press, 1982: 187-193. (in Chinese)

[2] 喻春明, 蒋金根, 熊和平, 郭运玲. 苎麻自交系选育及其配合力研究初报. 作物杂志, 1992(3): 14-16.

Yu C M, Jiang J G, Xiong H P, Guo Y L. Studies on breeding and combining ability of ramie inbred line. Crops, 1992(3): 14-16. (in Chinese)

[3] 彭定祥. 芋麻自交纯化的选择方法. 华中农业大学学报, 1993, 12(2): 106-111.

Peng D X. Selection method on Homozygous of ramie inbred. Journal of Huazhong Agricultural University, 1993, 12(2): 106-111. (in Chinese)

[4] 熊和平, 蒋金根, 喻春明, 郭运玲. 苎麻纯系培育和杂种优势预测的研究. 中国农业科学, 1995, 28(5): 54-64.

Xiong H P, Jiang J G, Yu C M, Guo Y L. Studies on acquisition of pure lines and forecast of heterosis in ramie. Scientia Agricultura Sinica, 1995, 28(5): 54-64. (in Chinese)

[5] 刘晶. 中国豆梨与川梨的遗传多样性和群体遗传结构研究[D]. 杭州: 浙江大学, 2013.

Liu J. Studies on genetic diversity and structure of Pvrus calleryans and P.pashia in China[D]. Hangzhou: Zhejiang University, 2013. (in Chinese)

[6] 王威威. 鹿蹄草遗传多样性的AFLP与SRAP分析[D]. 哈尔滨: 东北农业大学, 2013.

Wang W W. Analysis of genetic diversity of Pyrola calliantha by AFLP and SRAP markers[D]. Harbin: Northeast Agricultural University, 2013. (in Chinese)

[7] 张博. 黄鲫分子标记开发及遗传多样性分析[D]. 舟山: 浙江海洋学院, 2013.

Zhang B. Analysis of genetic diversity in Setipinna taty germplasms[D].Zhoushan: Zhejiang Ocean University, 2013. (in Chinese)

[8] 李铎, 柴志欣, 姬秋梅, 张成福, 信金伟, 钟金城. 西藏牦牛微卫星DNA的遗传多样性. 遗传, 2013, 35(2): 175-184.

Li D, Chai Z X, Ji Q M, Zhang C F, Xin J W, Zhong J C. Genetic diversity of DNA microsatellite for Tibetan Yak. Hereditas, 2013, 35(2): 175-184. (in Chinese)

[9] 赖勇, 王鹏喜, 范贵强, 司二静, 王晋, 杨轲, 孟亚雄, 李葆春, 马小乐, 尚勋武, 王化俊. 大麦SSR标记遗传多样性及其与农艺性状关联分析. 中国农业科学, 2013, 46(2): 233-242.

Lai Y, Wang P X, Fan G Q, Si E J, Wang J, Yang K, Meng Y X, Li B C, Ma X L, Shang X W, Wang H J. Genetic diversity and association analysis using SSR markers in barley. Scientia Agricultura Sinica, 2013, 46(2): 233-242. (in Chinese)

[10] 周建林, 揭雨成, 蒋彦波, 胡翔, 张健. 用微卫星DNA标记分析苎麻品种的亲缘关系. 作物学报, 2004, 30(3): 289-292.

Zhou J L, Jie Y C, Jiang Y B, Hu X, Zhang J. Genetic relation analysis on ramie cultivars with microsatellite markers. Acta Agronomica Sinica, 2004, 30(3): 289-292. (in Chinese)

[11] 蒙祖庆, 刘立军, 彭定祥. 利用RAPD和ISSR标记分析苎麻野生种质资源的遗传多样性. 分子植物育种, 2009, 7(2): 365-370.

Meng Z Q, Liu L J, Peng D X. Analysis on genetic diversity of ramie(Boehmeria nivea L.) wild germplasm by RAPD and ISSR markers. Molecular Plant Breeding, 2009, 7(2): 365-370. (in Chinese)

[12] 温岚, 喻春明, 王延周, 陈平, 陈继康, 谭龙涛, 熊和平. 苎麻多胚苗遗传多样性的SRAP标记分析. 湖南农业大学学报: 自然科学版, 2011, 37(3): 243-247.

Wen L, Yu C M, Wang Y Z, Chen P, Chen J K, Tan L T, Xiong H P. Application of molecular marker SRAP on analysis of genetic diversity of polyembryonic ramie seedings. Journal of Hunan Agricultural University: Natural Sciences, 2011, 37(3): 243-247. (in Chinese)

[13] 陈平, 谭龙涛, 喻春明, 王延周, 陈继康, 熊和平. 一种适用于PCR检测的苎麻陈年原麻DNA提取方法. 中国麻业科学, 2012, 34(6): 249-251.

Chen P, Tan L T, Yu C M, Wang Y Z, Chen J K, Xiong H P. Method of extracting DNA from aged ramie raw fiber for PCR detecting. Plant Fiber Sciences in China, 2012, 34(6): 249-251. (in Chinese)

[14] 谭龙涛. 苎麻自交纯合进度评价研究[D]. 北京: 中国农业科学院, 2012.

Tan L T. Study on homozygous progress evaluation of self-pollination in ramie(Boehmeria Nivea L.)[D]. Beijing: Chinese Academy of Agricultural Sciences, 2012. (in Chinese)

[15] 谭龙涛, 喻春明, 陈平, 王延周, 陈继康, 温岚, 熊和平. 样本量对中苎1号群体遗传多样性参数的影响. 中国麻业科学, 2012, 34(4): 179-183.

Tan L T, Yu C M, Chen P, Wang Y Z, Chen J K, Wen L, Xiong H P. Effects of sample size on genetic diversity parameters for ‘Zhong Zhu No.1’group. Plant Fiber Sciences in China, 2012, 34(4): 179-183. (in Chinese)

[16] Yeh F C, Yang R C, Boyle T. Popgene Version 1.31 Quick User Guide. Canada: University of Alberta, and Centre for International Forestry Research, 1999.

[17] Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, 155(2): 945-959.

[18] Rohlf F J. Numerical Taxonomy and Multivariate Analysis System NTSYS-pc version 2.1 User Guide. New York: Exeter Software Setauket, 2000.

[19] Yeh F C, Boyle T J B. Population genetic analysis of codominant and dominant markers and quantitative traits. Belgian Journal of Botany, 1997, 129(1): 157.

[20] Excofier L, Smouse P E, Quattro J M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: Applications to human mitochondrial DNA restriction data. Genetics, 1992, 131(3): 479-291.

[21] Powell W M, Morgante C, Andre M, Hanafey J, Vogel S, Tingey A, Rafalski A. Comparison of RFLP, RAPD, AFLP and SSR (microsatellite) makers for germplasm analysis. Molecular Breeding, 1996, 2(3): 225-238.

[22] 王东娜, 牟长城, 高卓. 胡桃楸天然种群遗传多样性的ISSR分析. 经济林研究, 2011, 29(2): 22-29.

Wang D N, Mu C C, Gao Z. ISSR analysis of genetic diversity of Juglans mandshurica maxim populations. Nonwood Forest Research, 2011, 29(2): 22-29. (in Chinese)

[23] Smykal P, Hybl M, Corander J, Jarkovsky J, Flavell A J, Griga M. Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theoretical and Applied Genetics, 2008, 117(3): 413-424.

[24] 章秋平, 刘冬成, 刘威生, 刘硕, 张爱民, 刘宁, 张玉萍. 华北生态群普通杏遗传多样性与群体结构分析. 中国农业科学, 2013, 46(1): 89-98.

Zhang Q P, Liu D C, Liu W S, Liu S, Zhang A M, Liu N, Zhang Y P. Genetic diversity and population structure of the north China populations of Apricot (Prunus armeniaca L.). Scientia Agricultura Sinica, 2013, 46(1): 89-98. (in Chinese)

[25] Lu H, Redus M A, Coburn J R, Rutger J N, McCouch S R, Tai T H. Population structure and breeding patterns of 145 US rice cultivars based on SSR marker analysis. Crop Science, 2005, 45(1): 66-76.

[26] 王立新, 季伟, 李宏博, 葛玲玲, 信爱华, 王丽霞, 常利芳, 赵昌平. 以DNA位点纯合率评价小麦品种的一致性和稳定性. 作物学报, 2009, 35(12):2197-2204.

Wang L X, Ji W. Li H B, Ge L L, Xin A H, Wang L X, Chang L F, Zhao C P. Evaluating uniformity and stability of wheat cultivars based on ratio of homozygous DNA locus. Acta Agronomica Sinica, 2009, 35(12): 2197-2204. (in Chinese)

[27] 廖亮, 李同建, 赵中伟, 陈玉波, 徐玲玲, 潘其辉, 石庆华. 基于SRAP分析苎麻及近缘种的系统学关系. 广西植物, 2010, 30(6): 791-795.

Liao L, Li T J, Zhao Z W, Chen Y B, Xu L L, Pan Q H, Shi Q H. Phylogenetic relationship of ramie and its wild relatives based on SRAP markers. Guihaia, 2010, 30(6): 791-795. (in Chinese)

[28] 郭阳, 熊和平, 陈平, 王延周, 陈继康, 谭龙涛, 郑建树, 喻春明. 中国主要苎麻品种遗传多样性的SSR标记分析. 湖南农业科学, 2012, 23(12): 4-6,9.

Guo Y, Xiong H P, Chen P, Wang Y Z, Chen J K, Tan L T, Zheng J S, Yu C M. Analysis of genetic diversity for main ramie varieties in China by SSR markers. Hunan Agricultural Sciences, 2012, 23(12): 4-6,9. (in Chinese)

[29] 王丽霞, 王立新, 季伟, 李宏博, 信爱华, 常利芳, 魏建民, 赵昌平. 小麦F₄代株系的DNA位点纯合率和农艺性状变异幅度. 分子植物育种, 2009, 7(4): 703-708.

Wang L X, Wang L X, Ji W, Li H B, Xin A H, Chang L F, Wei J M, Zhao C P. The homozygous DNA locus ratio and the variation range of agronomic traits of wheat F₄lines. Molecular Plant Breeding, 2009, 7(4): 703-708. (in Chinese)

[30] 王春平, 许时伦, 吕静霞, 赵香连. 三三连锁杂合自交群体基因纯合效应的定量模型研究. 种子, 2013, 32(6): 31-35.

Wang C P, Xu S L, Lü J X, Zhao X L. Quantitative model of the homozygous effect of heterogenes in linkage of the selfing population. Seed, 2013, 32(6): 31-35. (in Chinese)

[31] 王立新, 常利芳, 李宏博, 葛玲玲, 信爱华, 高世庆, 季伟, 孙辉, 赵昌平. 小麦种子纯度的分子标记检测方法. 麦类作物学报, 2009, 29(1): 1-8.

Wang L X, Chang L F, Li H B, Ge L L, Xin A H, Gao S Q, Ji W, Sun H, Zhao C P. Method of testing wheat seeds purity by molecular markers. Journal of Triticeae Crops, 2009, 29(1): 1-8. (in Chinese)

[32] 何晓红, 韩秀丽, 关伟军, 田可川, 张文彬, 马月辉. 采用微卫星标记分析10个双峰驼群体的遗传变异和群体间的遗传关系. 生物多样性, 2012, 20(2): 199-206.

He X H, Han X L, Guan W J, Tian K C, Zhang W B, Ma Y H. Genetic variability and relationship of 10 bactrian camel populations revealed by microsatellite markers. Biodiversity Science, 2012, 20(2): 199-206. (in Chinese)

[33] 蒋维昕. 毛竹的遗传结构及群体演化[D]. 南京: 南京林业大学, 2013.

Jiang W X. Population structure and phylogenetic relationships of Phyllostachys edulis(Poaceae) in China[D]. Nanjing: Nanjing Forestry University, 2013. (in Chinese)

[34] 张含国. 红皮云杉遗传多样性的研究[D]. 哈尔滨: 东北林业大学, 2000.

Zhang H G. Study on the genetic diversity of Picea koraiensis[D].Harbin:Northeast Forestry University, 2000. (in Chinese)

[35] 孙孝德, 孙国华, 袁廷柱, 杨建敏, 王卫军, 吉成龙, 宋志乐. 刺参(Apostichopus japonicus)野生及两代选育群体间遗传变异的微卫星标记研究. 海洋与湖沼, 2011, 42(3): 380-386.

Sun X D, Sun G H, Yuan T Z, Yang J M, Wang W J, Ji C L, Song Z L. Genetic variations of wild and selectively bred populations of a sea cucumber Apostichopus japonicus. Oceanologia Et Limnlolgia Sinica, 2011, 42(3): 380-386. (in Chinese)

[36] 俞靓, 井赵斌, 程积民. 陕西省本氏针茅自然种群遗传多样性的ISSR分析. 草地学报, 2012, 20(3): 512-517.

Yu L, Jing Z B, Cheng J M. Genetic diversity of Stipa bungeana trin. populations in Shanxi analyzed by ISSR markers. Acta Agrestia Sinica, 2012, 20(3): 512-517. (in Chinese)

[37] Wright S. The genetical structure of populations. Annals of Eugenices, 1951, 15(1): 323-354.

[38] Milbourne D, Meyer R, Bradshaw J E, Baird E, Bonar N, Provan J, Powell W, Waugh R. Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato. Molecular Breeding, 1997, 2(3): 127-136.

[39] 李永祥, 李斯深, 李立会, 杨欣明, 李秀全. 披碱草属12个物种遗传多样性的ISSR和SSR比较分析. 中国农业科学, 2005, 38(8): 1522-1527.

Li Y X, Li S S, Li L H, Yang X M, Li X Q. Comparison of genetic diversity of twelve elymus species using ISSR and SSR markers. Scientia Agricultura Sinica, 2005, 38(8): 1522-1527. (in Chinese)

[40] Archak S, Gaikwad A B, Gautam D, Rao E V V B, Swamy K R M, Karihaloo J L. Comparative assessment of DNA fingerprinting techniques(RAPD, ISSR and AFLP) for genetic analysis of cashew(Anacardium occidentale L.) accessions of India. Genome, 2003, 46(3): 362-369.

[41] 钱韦, 葛颂, 洪德元. 采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性. 植物学报, 2000, 42(7): 741-750.

Qian W, Ge S, Hong D Y. Assessment of genetic variation of Oryza granulate detected by RAPDs and ISSRs. Acta Botanica Sinica, 2000, 42(7): 741-750. (in Chinese)

[42] Sun G L, Salomon B, von Bothmer R. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers. Genome, 1997, 40(6): 806-814.

[43] MacRitchie D, Sun G L. Evaluating the potential of barley and wheat microsatellite markers or genetic analysis of Elymus trachycaulus complex species. Theoretical and Applied Genetics, 2004, 108(4): 720-724.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Study on Homozygous Progress Evaluation of Self-Pollination in Ramie (Boehmeria Nivea L.)

RichHTML

PDF

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	ZHU YanSong,ZHANG YaFei,CHENG Li,YANG ShengNan,ZHAO WanTong,JIANG Dong. Identification of 60 Citrus Accessions Using Target SSR-seq Technology [J]. Scientia Agricultura Sinica, 2022, 55(22): 4458-4472.
[2]	JIANG Peng, ZHANG Peng, YAO JinBao, WU Lei, HE Yi, LI Chang, MA HongXiang, ZHANG Xu. Phenotypic Characteristics and Related Gene Analysis of Ningmai Series Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(2): 233-247.
[3]	XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[4]	YingLing WAN,MengTing ZHU,AiQing LIU,YiJia JIN,Yan LIU. Phenotypic Diversity Analysis of Chinese Ornamental Herbaceous Peonies and Its Germplasm Resource Evaluation [J]. Scientia Agricultura Sinica, 2022, 55(18): 3629-3639.
[5]	HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[6]	YANG Cheng,GONG GuiZhi,PENG ZhuChun,CHANG ZhenZhen,YI Xuan,HONG QiBin. Genetic Relationship Among Citrus and Its Relatives as Revealed by cpInDel and cpSSR Marker [J]. Scientia Agricultura Sinica, 2022, 55(16): 3210-3223.
[7]	WANG LuWei,SHEN ZhiJun,LI HeHuan,PAN Lei,NIU Liang,CUI GuoChao,ZENG WenFang,WANG ZhiQiang,LU ZhenHua. Analysis of Genetic Diversity of 79 Cultivars Based on SSR Fluorescence Markers for Peach [J]. Scientia Agricultura Sinica, 2022, 55(15): 3002-3017.
[8]	CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[9]	XU Xiao,REN GenZeng,ZHAO XinRui,CHANG JinHua,CUI JiangHui. Accurate Identification and Comprehensive Evaluation of Panicle Phenotypic Traits of Landraces and Cultivars of Sorghum bicolor (L.) Moench in China [J]. Scientia Agricultura Sinica, 2022, 55(11): 2092-2108.
[10]	SUN Yue,YANG HuiMin,HE RongRong,ZHANG JunXiang. Implantation and Persistence of Inoculated Active Dry Yeast in Industrial Wine Fermentations [J]. Scientia Agricultura Sinica, 2021, 54(9): 2006-2016.
[11]	NIE XingHua, ZHENG RuiJie, ZHAO YongLian, CAO QingQin, QIN Ling, XING Yu. Genetic Diversity Evaluation of Castanea in China Based on Fluorescently Labeled SSR [J]. Scientia Agricultura Sinica, 2021, 54(8): 1739-1750.
[12]	TANG XiuJun,FAN YanFeng,JIA XiaoXu,GE QingLian,LU JunXian,TANG MengJun,HAN Wei,GAO YuShi. Genetic Diversity and Origin Characteristics of Chicken Species Based on Mitochondrial DNA D-loop Region [J]. Scientia Agricultura Sinica, 2021, 54(24): 5302-5315.
[13]	HU DongMei,JIANG Dong,LI YongPing,PENG Lei,LI DongYun,ZHU YanSong,YANG YunGuang. Identification of Bud Sport Mutation of Satsuma Mandarin by Target SSR-seq Technology [J]. Scientia Agricultura Sinica, 2021, 54(23): 5083-5096.
[14]	WANG Yan,FAN BaoJie,CAO ZhiMin,ZHANG ZhiXiao,SU QiuZhu,WANG Shen,WANG XueQing,PENG XiuGuo,MEI Li,WU YuHua,LIU ShaoXing,TIAN ShengMin,XU JunJie,JIANG ChunZhi,WANG WeiJuan,LIU ChangYou,TIAN Jing. Quantitative Trait Locus Mapping of Bruchids Resistance Based on A Novel Genetic Linkage Map in Cowpea (Vigna unguiculata) [J]. Scientia Agricultura Sinica, 2021, 54(22): 4740-4749.
[15]	LI XinYuan, LOU JinXiu, LIU QingYuan, HU Jian, ZHANG YingJun. Genetic Diversity Analysis of Rhizobia Associated with Medicago sativa Cultivated in Northeast and North China [J]. Scientia Agricultura Sinica, 2021, 54(16): 3393-3405.