Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (14): 2762-2773.doi: 10.3864/j.issn.0578-1752.2014.14.007

• PLANT PROTECTION • Previous Articles     Next Articles

Biological Characteristics and Genetic Diversity of Ustilaginoidea virens from Rice Regions in China

 WANG  Wen-Bin-1, 2 , ZHANG  Rong-Sheng-2, LUO  Chu-Ping-2, YIN  Xiao-Le-2, LIU  Yong-Feng-2, LU  Fan-1, 2 , CHEN  Zhi-Yi-1, 2   

  1. 1、College of Plant Protection, Nanjing Agricultural University, Nanjing 210095;
    2、Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2014-01-24 Online:2014-07-15 Published:2014-04-02

RichHTML

3

PDF

940

Cited

2

Abstract: 【Objective】The objective of this study is to reveal the relationship among geographical regions, biological characteristics and genetic diversity of 111 strains of Ustilaginoidea virens from 10 provinces in China.【Method】Sporulation, growth rate and pathogenicity of these strains were evaluated by biological methods, and the relativity was analyzed by using the software SPSS 20.0. DNA was extracted from the strains, the genomic fingerprint profiles of 111 strains were generated by BOX-PCR, REP-PCR and ERIC-PCR, respectively. Their genetic diversities were further investigated by using clustering analysis.【Result】The correlation coefficient of sporulation and pathogenicity was 0.765 and the significance probability was 0.001, the correlation coefficient of growth rate and pathogenicity was 0.035 and the significance probability was 0.685. The genomic fingerprint profiles of 111 strains were generated by BOX-PCR, REP-PCR and ERIC-PCR, respectively. The genetic diversities of the U. virens population were 0.73 (in BOX), 0.62 (in ERIC) and 0.60 (in REP), respectively. The number of polymorphic bands was between 7 and 13 of each examined isolate detected through BOX-PCR analysis. According to cluster analysis of UPGMA, 6 clusters were grouped based on the boundary level of 0.70 average distances. The predominant group was group one which included 57 strains (51.4%). The strains of U. virens from Anhui, Sichuan, Guangxi, Hubei and Yunnan were grouped into group 1 and group 4. The strains of group 2 were mainly from Jiangsu, including 18 strains (16.2%), the strains of group 3 were from Zhejiang, including 8 strains (7.2%), the strains of group 5 were from Heilongjiang, including 9 strains (8.1%), and the strains of group 6 were from Liaoning, including 12 strains (10.8%). There was some correlation between BOX groups and geographic regions, no relationships were observed between BOX groups and their biological characteristics.【Conclusion】The relativity between the sporulation and pathogenicity was assumed to be medium. There was no relativity between the growth rate and pathogenicity. There was a significant relationship between BOX groups and the geographical regions, these results suggested that, naturally, U. virens in China might not be spread over long distances. No significant relationships were observed between BOX groups and their biological characteristics.

Key words: Ustilaginoidea virens , biological characteristics , geographical regions , BOX-PCR , genetic diversity

[1]Tanaka E, Ashizawa K, Sonoda R, Tanaka C. Villosiclava virens gen. nov., comb. nov., teleomorph of Ustilaginoidea virens, the causal agent of rice false smut. Mycotaxon, 2008, 106(1): 491-501.

[2]胡东维, 王疏. 稻曲病菌侵染机制研究现状与展望. 中国农业科学, 2012, 45(22): 4604-4611.

Hu D W, Wang S. Progress and perspectives in infection mechanism of Ustilaginoidea virens. Scientia Agricultura Sinica, 2012, 45(22): 4604-4611. (in Chinese)

[3]王大为, 王疏, 傅俊范. 稻曲病研究进展. 辽宁农业科学, 2004(1): 21-24.

Wang D W, Wang S, Fu J F. Research advance on false smut of rice. Liaoning Agricultural Sciences, 2004(1): 21-24. (in Chinese)

[4]黄世文, 余柳青. 国内稻曲病的研究现状. 江西农业学报, 2002, 14(2): 45-51.

Huang S W, Yu L Q. Present situation of studies on rice false smut (Ustilaginoidea virens) in China. Acta Agriculturae Jiangxi, 2002, 14(2): 45-51. (in Chinese)

[5]王洪凯, 林福呈. 稻曲病研究进展. 浙江农业学报, 2008, 20(5): 385-390.

Wang H K, Lin F C. Research advances in rice false smut, Ustilaginoiden virens. Acta Agriculturae Zhejiangensis, 2008, 20(5): 385-390. (in Chinese)

[6]Koiso Y, Li Y, Iwasaki S, Hanaoka K, Kobayashi T, Sonoda R, Fujita Y, Yaegashi H, Sato Z. Ustiloxins, antimitotic cyclic peptides from false smut balls on rice panicles caused by Ustilaginoidea virens. The Journal of Antibiotics, 1994, 47(7): 765-773.

[7]Li Y, Koiso Y, Kobayashi H, Hashimoto Y, Iwasaki S. Ustiloxins, new antimitotic cyclic peptides: interaction with porcine brain tubulin. Biochemical Pharmacology, 1995, 49(10): 1367-1372.

[8]周永力, 樊金娟, 曾超珍, 刘小舟, 王疏, 赵开军. 稻曲病菌遗传多样性与群体结构的初步分析. 植物病理学报, 2004, 34(5): 442-448.

Zhou Y L, Fan J J, Zeng C Z, Liu X Z, Wang S, Zhao K J. Preliminary analysis of genetic diversity and population structure of Ustilaginoidea virens. Acta Phytopathologica Sinica, 2004, 34(5): 442-448. (in Chinese)

[9]李燕, 刘永锋, 张荣胜, 俞咪娜, 陈志谊. 江苏省稻曲病菌的群体遗传结构. 江苏农业学报, 2011, 28(2): 296-301.

Li Y, Liu Y F, Zhang R S, Yu M N, Chen Z Y. Genetic diversity of Ustilaginoidea virens from rice in Jiangsu Province. Jiangsu Journal of Agricultural Sciences, 2011, 28(2): 296-301. (in Chinese)

[10]张敏, 李竞生, 刘坚, 宋伟, 代海霞. 四川省籼稻区水稻稻曲病菌遗传多样性分析. 植物保护学报, 2009, 36(2): 113-118.

Zhang M, Li J S, Liu J, Song W, Dai H X. Preliminary analysis of genetic diversity of Ustilaginoidea virens strains of indica rice from Sichuan Province. Acta Phytophylacica Sinica, 2009, 36(2): 113-118. (in Chinese)

[11]俞咪娜, 陈志谊, 于俊杰, 胡建坤, 尹小乐, 聂亚锋, 刘永锋. 来源于同一穗不同稻曲球的稻曲病菌的致病性及遗传多样性. 植物病理学报, 2013, 43(6): 561-573.

Yu M N, Chen Z Y, Yu J J, Hu J K, Yin X L, Nie Y F, Liu Y F. Genetic diversity and pathogenicity of Ustilaginoidea virens isolated from different rice false smut balls of a diseased spike. Acta Phytopathologica Sinica, 2013, 43(6): 561-573. (in Chinese)

[12]Redondo C, Cubero J, Melgarejo P. Characterization of Penicillium species by ribosomal DNA sequencing and BOX, ERIC and REP-PCR analysis. Mycopathologia, 2009, 168(1): 11-22.

[13]Vicente J G, Roberts S J. Discrimination of Pseudomonas syringae isolates from sweet and wild cherry using rep-PCR. European Journal of Plant Pathology, 2007, 117(4): 383-392.

[14]李燕, 尹小乐, 刘永锋, 于俊杰, 陈志谊. 稻曲病菌生物学特性与致病力相关性研究. 植物病理学报, 2012, 42(4): 353-364.

Li Y, Yin X L, Liu Y F, Yu J J, Chen Z Y. Relativity of biological characteristics and pathogenicity of Ustilaginoidea virens. Acta Phytopathologica Sinica, 2012, 42(4): 353-364. (in Chinese)

[15]刘明霞, 秦姝, 李颖, 王疏. 稻曲病菌分离技术、培养条件研究. 辽宁农业科学, 2009(6): 20-22.

Liu M X, Qin S, Li Y, Wang S. Study on isolation technique and culture condition of Ustilaginoidea virens. Liaoning Agricultural Sciences, 2009(6): 20-22. (in Chinese)

[16]王永强, 樊荣辉, 杨炳辉, 刘兵, 张敬泽, 胡东维. 稻绿核菌(稻曲病菌)分离方法的比较研究. 菌物学报, 2010, 29(1): 59-63.

Wang Y Q, Fan R H, Yang B H, Liu B, Zhang J Z, Hu D W. Comparison of isolation methods for Ustilaginoidea virens, the pathogen of rice false smut. Mycosystema, 2010, 29(1): 59-63. (in Chinese)

[17]李燕, 于俊杰, 刘永锋, 尹小乐, 张荣胜, 俞咪娜, 陈志谊. 稻曲病菌产孢能力及致病力测定. 中国农业科学, 2012, 45(20): 4166-4177.

Li Y, Yu J J, Liu Y F, Yin X L, Zhang R S, Yu M N, Chen Z Y. Determination of sporulation and pathogenicity of Ustilaginoidea virens. Scientia Agricultura Sinica, 2012, 45(20): 4166-4177. (in Chinese)

[18]张君成, 陈志谊, 张炳欣, 刘永锋, 陆凡. 稻曲病的接种技术研究. 植物病理学报, 2004, 34(5): 463-467.

Zhang J C, Chen Z Y, Zhang B X, Liu Y F, Lu F. Inoculation techniques used for inducing rice false smut efficiently. Acta Phytopathologica Sinica, 2004, 34(5): 463-467. (in Chinese)

[19]曾列先, 陈深, 张慧, 潘汝谦, 杨健源, 伍圣远, 翟培茹, 朱小源. 广东水稻白叶枯病菌遗传多样性和小种分化研究. 植物病理学报, 2009, 39(3): 231-237.

Zeng L X, Chen S, Zhang H, Pan R Q, Yang J Y, Wu S Y, Zhai P R, Zhu X Y. Genetic diversity and variability of Xanthomonas oryzae pv. oryzae in Guangdong. Acta Phytopathologica Sinica, 2009, 39(3): 231-237. (in Chinese)

[20]张荣胜, 陈志谊, 刘永锋. 水稻细菌性条斑病菌遗传多样性和致病型分化研究. 中国水稻科学, 2011, 25(5): 523-528.

Zhang R S, Chen Z Y, Liu Y F. Genetic diversity and pathotype variability of Xanthomonas oryzae pv. oryzicola strains. Chinese Journal of Rice Science, 2011, 25(5): 523-528. (in Chinese)

[21]周益军, 白娟, 程兆榜, 范永坚, 熊如意, 王跃. 中国稻瘟病菌群体多样性研究. 中国水稻科学, 2004, 18(3): 277-280.

Zhou Y J, Bai J, Cheng Z B, Fan Y J, Xiong R Y, Wang Y. Population structure of Magnaporthe grisea from rice in China. Chinese Journal of Rice Science, 2004, 18(3): 277-280. (in Chinese)

[22]林琳, 姬广海, 马改转, 王永吉, 张丽辉. 西南地区水稻细菌性条斑病菌的遗传多样性分析. 江西农业大学学报, 2011, 33(2): 264-269.

Lin L, Ji G H, Ma G Z, Wang Y J, Zhang L H. Genotypic diversity analysis of Xanthomonas oryzae pv. oryzicola in Southwest China by Rep-PCR. Acta Agriculturae Universitatis Jiangxiensis, 2011, 33(2): 264-269. (in Chinese)

[23]Sun X Y, Kang S, Zhang Y J, Tan X Q, Yu Y F, He H Y, Zhang X Y, Liu Y F, Wang S, Sun W X, Cai L, Li S J. Genetic diversity and population structure of rice pathogen Ustilaginoidea virens in China. PloS One, 2013, 8(9): e76879.

[24]王舒婷, 林廷邦, 甘林, 石妞妞, 杨秀娟, 陈福如. 中国部分地区稻曲病菌培养特性及其遗传多样性分析. 植物保护学报, 2012, 39(3): 217-223.

Wang S T, Lin T B, Gan L, Shi N N, Yang X J , Chen F R. Analysis of cultural characteristics and genetic diversity of Ustilaginoidea virens from some regions in China. Acta Phytophylacica Sinica, 2012, 39(3): 217-223. (in Chinese)

[25]卢代华, 陈元平, 王剑, 王平, 姚琳, 叶慧丽, 胡容平, 陈宇, 毛建辉. 四川省稻曲病菌的群体遗传结构. 西南农业学报, 2013, 26(3): 994-1000.

Lu D H, Chen Y P, Wang J, Wang P, Yao L, Ye L H, Hu R P, Chen Y, Mao J H. Genetic diversity of Ustilaginoidea virens from rice in Sichuan Province. Southwest China Journal of Agricultural Sciences, 2013, 26(3): 994-1000. (in Chinese)

[26]潘雅姣, 樊金娟, 付彬英, 王洁, 徐建龙, 陈红漫, 黎志康, 周永力. 采用AFLP技术分析稻曲病菌的遗传多样性I: 同一块稻田中稻曲病菌的遗传结构. 植物病理学报, 2006, 36(4): 337-341.

Pan Y J, Fan J J, Fu B Y, Wang J, Xu J L, Chen H M, Li Z K, Zhou Y L. Genetic diversity of Ustilaginoidea virens revealed by AFLP Ⅰ: genetic structure of the pathogen in a field. Acta Phytopathologica Sinica, 2006, 36(4): 337-341. (in Chinese)
[1] HE ZhiLin, SUN CuiXia, YUE HongLi, TAN YueXia, ZHANG YaoHai, WANG FuSheng, LIU SiTao, JIANG Dong. Genetic Diversity Analysis and GWAS of Alloocimene Based on Resequencing of Citron, Lemon Germplasm Resources [J]. Scientia Agricultura Sinica, 2026, 59(2): 386-401.
[2] XU YuJuan, ZHANG Jie, WANG TianYi, CHEN HaoYang, ZHAO JiaJia, WU BangBang, HAO YuQiong, LI XiaoHua, ZHENG XingWei, ZUO JingJing, ZHENG Jun. Identification of Glu-A3 and Glu-B3 of Low-Molecular-Weight Glutenin in Shanxi Wheat and Its Effect on Quality [J]. Scientia Agricultura Sinica, 2025, 58(24): 5110-5127.
[3] CHEN CaiJin, MA Lin, JIANG QingXue, LIU JinHui, MIAO Tong, ZHANG ZhiPeng, MENG Xiang, MA XiaoRan, ZHOU XinYue, ZHANG Jian, LIU WenHui, WANG XueMin. Genetic Diversity Analysis of Phenotypic Traits of 244 Forage Oat Germplasm Resources [J]. Scientia Agricultura Sinica, 2025, 58(23): 4825-4836.
[4] WEI YiMin, ZHOU MeiLiang, TANG Yu. Origin, Evolution and Spread of Crop Buckwheat [J]. Scientia Agricultura Sinica, 2025, 58(21): 4305-4316.
[5] LIU XiaoXu, ZHONG ZeXin, QIU JiaRen, YANG ChunXiao, ZHANG YongJun, XIE Wen, ZHANG YouJun, PAN HuiPeng. GENETIC DIVERSITY OF MTCO1 IN DIFFERENT GEOGRAPHICAL POPULATIONS OF MEGALUROTHRIPS USITATUS [J]. Scientia Agricultura Sinica, 2025, 58(21): 4361-4371.
[6] GUO MengZe, ZHANG Lei, SUN PingPing, JIANG Biao, YAN JinQiang, LI ZhengNan. Molecular Characterization and Evolutionary Dynamics of Tomato Leaf Curl New Delhi Virus Isolate from Wax Gourd (Benincasa hispida) in Guangdong [J]. Scientia Agricultura Sinica, 2025, 58(19): 3890-3904.
[7] LIU PengPeng, LI JiangBo, XU HongJun, NIE YingBin, HAN XinNian, KONG DeZhen, SANG Wei. Genetic Diversity Analysis of Protein Fractions and Quality in Xinjiang Winter Wheat Cultivar Resources [J]. Scientia Agricultura Sinica, 2025, 58(15): 2948-2959.
[8] WANG Hui, DING BaoPeng, LI YuXian, REN QuanRu, ZHOU Hai, ZHAO JunLiang, HU HaiFei. Research Progress and Prospects on Crop Pan-Genomics [J]. Scientia Agricultura Sinica, 2025, 58(11): 2045-2061.
[9] LI Pei, HE ZhiLin, TAN YueXia, ZHAO WanTong, FENG JinYing, CHEN GuiHu, YAN Chi, WANG ZiHao, HUANG Ping, JIANG Dong. Genetic Diversity Analysis of Mandarin and Excellent Germplasm Screening Based on Whole-Genome Resequencing Data and Phenotypic Traits [J]. Scientia Agricultura Sinica, 2024, 57(23): 4761-4773.
[10] YANG Chun, YANG DaiXing, LI Yan, LIANG SiHui, DENG XiaoQiang, QIAO DaHe, CHEN Juan, GUO Yan, LIN KaiQin, CHEN ZhengWu. Comprehensive Analysis of Morphologic Characters and Biochemical Components of Guizhou Dashu Tea Germplasms [J]. Scientia Agricultura Sinica, 2024, 57(19): 3894-3916.
[11] LI YuShan, XIAO Jing, MA Yue, TIAN Chao, ZHAO LianJia, WANG Fan, SONG Yu, JIANG ChengYao. Identification and Evaluation of Phenotypic Characters and Genetic Diversity Analysis of 169 Tomato Germplasm Resources [J]. Scientia Agricultura Sinica, 2024, 57(18): 3671-3683.
[12] ZHAI CaiJiao, GE LiJiao, CHENG YuJing, QIU Liang, WANG XiaoQiu, LIU ShuiDong. Genetic Diversity Analysis of Wax Gourd and Chieh-Qua Germplasm Resources Based on Phenotypic Traits and SSR Markers [J]. Scientia Agricultura Sinica, 2024, 57(17): 3440-3457.
[13] LEI MengLin, LIU Xia, WANG YanZhen, CUI GuoQing, MU ZhiXin, LIU LongLong, LI Xin, LU LaHu, LI XiaoLi, ZHANG XiaoJun. Genetic Diversity Analysis of Winter Wheat Germplasm Resources in Shanxi Province Based on 55K SNP Array [J]. Scientia Agricultura Sinica, 2024, 57(10): 1845-1856.
[14] ZHANG YiZhong, ZHANG XiaoJuan, LIANG Du, GUO Qi, FAN XinQi, NIE MengEn, WANG HuiYan, ZHAO WenBo, DU WeiJun, LIU QingShan. Genetic Diversity Analysis and Comprehensive Evaluation of Sorghum Breeding Materials Based on Phenotypic Traits [J]. Scientia Agricultura Sinica, 2023, 56(15): 2837-2853.
[15] LI Huan, YAN XiaoQing, YANG ZhanLie, TAN JinYu, LI XiaoBing, CHEN NengGang, WU RongJu, CHEN HuiCha, RUAN RenChao. Analysis and Comprehensive Evaluation of Phenotype Genetic Diversity in Kam Sweet Rice Germplasm Resources in Guizhou [J]. Scientia Agricultura Sinica, 2023, 56(11): 2035-2046.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd