Scientia Agricultura Sinica ›› 2014, Vol. 47 ›› Issue (14): 2774-2783.doi: 10.3864/j.issn.0578-1752.2014.14.008

• PLANT PROTECTION • Previous Articles     Next Articles

Expression and Analysis of β-1, 3-Glucanase Gene in Wheat TcLr35 Induced by Wheat Leaf Rust Pathogen and Signal Molecule

 LI  Xiao-Ying, GAO  Lin, ZHANG  Yan-Jun, WANG  Hai-Yan, LIU  Da-Qun   

  1. College of Plant Protection, Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, Baoding 071000, Hebei
  • Received:2014-01-13 Online:2014-07-15 Published:2014-04-26

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Abstract: 【Objective】β-1, 3-glucanase, a kind of pathogenesis-related protein, plays an important role in signal transduction in plants. The objective of this study is to understand the expression patterns of β-1, 3-glucanase gene in the interaction between Puccinia triticina and wheat TcLr35, identify the relativity between the aim gene and resistance to leaf rust pathogen attack, and explore the molecular mechanism of PRs mediated resistance in adult wheat to P. triticina.【Method】Based on the previous studies, the structure characteristics of β-1, 3-glucanase gene (TaLr35PR2) were analyzed using bioinformatics methods. In the meanwhile, the gene expression profiles of TaLr35PR2 induced by P. triticina and chemical molecule at different time points were analyzed by semi-quantitative RT-PCR method combining genetools and SPSS software. In addition, the similar analysis was also conducted on the different tissues of wheat including roots, stems and leaves, and different growth stages of wheat.【Result】TaLr35PR2 coded an alkaline protein positioned in the vacuole contained a signal peptide. The protein sequence of TaLr35PR2 had high homology with other plants, and had the closest relationship with pathogenesis-related protein 2 gene in Triticum aestivum, but farthest relationship with the PR protein in rice. The expression profile analysis of TaLr35PR2 at the adult stage in wheat at different time points after P. triticina inoculation indicated that the transcription level changed little in the compatible interaction (Thatcher), but changed much in the incompatible interaction (TcLr35). The transcription level was much earlier and higher in the incompatible interaction than that in the compatible interaction at different points. Different tissues of the adult wheat TcLr35 detection results showed that the gene expression in leaves increased significantly with the extension of inoculation time, the expression level reached a peak at 48 hpi, but the gene was not expressed in roots at different time points, and there was a little expression level at 48 hpi in stems and changed a little at the later period, so its expression abundance was leaf＞stem＞root. In different periods of wheat growth and development, the TaLr35PR2 expressed at certain levels without inoculation both in TcLr35 and Thatcher except at one-leaf stage, and the expression levels were much higher both in the incompatible and compatible interactions than that in the Mock reaction, then expressed stably at the adult stage. In addition, the expression level of the TaLr35PR2 increased significantly after pre-treatments with chemical molecule such as salicylic acid (SA) and abscisic acid (ABA).【Conclusion】The expression of TaLr35PR2 was induced by P. triticina. TaLr35PR2 may be involved in the defense response against rust pathogen and participate in SA, ABA mediated signaling pathway and take part in defense reaction to P. triticina of wheat TcLr35 adult stage.

Key words: wheat , pathogenesis-related protein (PRP) , signal molecule , Puccinia triticina , expression and analysis

[1]Zhang G, Li Y M, Zhang Y, Dong Y L, Wang X J, Wei G R, Huang L L, Kang Z S. Cloning and characterization of a pathogenesis-related protein gene TaPR10 from wheat induced by stripe rust pathogen. Agricultural Sciences in China, 2010, 9(4): 549-556.

[2]王海燕, 刘大群, 杨文香, 李在峰, 张立荣, 李星. TcLr35小麦β (1, 3; 1, 4) 葡聚糖苷酶基因cDNA全长的克隆及分析. 华北农学报, 2010, 25(4): 25-29.

Wang H Y, Liu D Q, Yang W X, Li Z F, Zhang L R, Li X. Cloning and characterization of β (1, 3; 1, 4) beta glucanase gene from TcLr35 wheat. Acta Agriculturae Boreali-Sinica, 2010, 25(4): 25-29. (in Chinese)

[3]Arlorio M, Ludwig A, Boller T. Inhibition of fungal growth by plant chitinases and β-1,3-glucanases: a morphological study. Protoplasma, 1992, 171: 34-43.

[4]王转梅, 陈崇顺, 王轶, 曹伟杰, 邹爱兰, 马文晔, 张娟. 烟草品种XanthiNN碱性β-1,3-葡聚糖酶基因的克隆与生物信息学分析. 江苏农业科学, 2009(1): 28-31.

Wang Z M, Chen C S, Wang Y, Cao W J, Zou A L, Ma W Y, Zhang J. Cloning and bioinformatics analysis of XanthiNN alkaline β-1,3 glucanase gene in tobacco varieties. Jiangsu Agricultural Sciences, 2009(1): 28-31. (in Chinese)

[5]Su Y C, Xu L P, Xue B T, Wu Q B, Guo J L, Wu L G, Que Y C. Molecular cloning and characterization of two pathogenesis related β-1,3-glucanase genes ScGluA1 and ScGluD1 from sugarcane infected by Sporisorium scitamineum. Plant Cell Report, 2013, 32: 1503-1519.

[6]翟国会, 阮小蕾, 吴丽婷, 谭小勇, 李华平. 指天蕉β-1, 3-葡聚糖酶基因全长cDNA的克隆及序列分析. 中国农业科学, 2011, 44(15): 3134-3141.

Zhai G H, Ruan X L, Wu L T, Tan X Y, Li H P. Cloning and sequence analysis of a full-length cDNA of β-1, 3-glucanase genes from Musa pisangawake. Scientia Agricultura Sinica, 2011, 44(15): 3134-3141. (in Chinese)

[7]李云锋, 朱蕊, 谢龙旭, 徐培林. 大麦β-1, 3-葡聚糖酶基因 (GⅢ) 启动子在转基因水稻中的诱导表达. 植物生理与分子生物学学报, 2005, 31(2): 143-148.

Li Y F, Zhu R, Xie L X, Xu P L. Inducible expression of a promoter of the gene encoding barely β-1,3 glucanase isoenzyme (GⅢ) in  transgenic rice. Journal of Plant Physiology and Molecular Biology, 2005, 31(2): 143-148. (in Chinese)

[8]蓝海燕, 王长海, 张丽华, 刘桂珍, 王岚兰, 陈正华, 田颖川. 导入β-1, 3-葡聚糖酶及几丁质酶基因的转基因可育油菜及其抗菌核病的研究. 生物工程学报, 2000, 16(2): 142-146.

Lan H Y, Wang C H, Zhang L H, Liu G Z, Wang L L, Chen Z H, Tian Y C. Study on transgenic oilseed rape (Brassica napus) plants transformed with β-1, 3-glucanase and chitinase gene and its resistance to Sclerotinia sclerotiorium. Chinese Journal of Biotechnology, 2000, 16(2): 142-146. (in Chinese)

[9]Sundaresha S, Manoj Kumar A, Rohini S, Math S A, Keshamma E, Chandrashekar S C, Udayakumar M. Enhanced protection against two major fungal pathogens of groundnut, Cercospora arachidicola and Aspergillus flavus in transgenic groundnut over-expressing a tobacco β-1,3-glucanase. European Journal of Plant Pathology, 2010, 126: 497-508.

[10]O’Kennedy M M, Crampton B G, Lorito M, Chakauya E, Breese W  A, Burger J T, Botha F C. Expression of a β-1,3-glucanase from a biocontrol fungus in transgenic pearl millet. South African Journal of Botany, 2011, 77: 335-345.

[11]Roy-Choudhury S, Roy S, Singh S K, Sengupta D N. Molecular characterization and differential expression of β-1,3-glucanase during ripening in banana fruit in response to ethylene, auxin, ABA, wounding, cold and light-dark cycles. Plant Cell Report, 2010, 29: 813-828.

[12]Anguelova-Merhar V S, VanDer Westhuizen A J, Pretorius Z A. β-1,3-glucanase and chitinase activities and the resistance response of wheat to leaf rust. Journal of Phytopathology, 2001, 149: 381-384.

[13]Zambounis A G, Kalamaki M S, Tani E E, Paplomatas E J, Tsaftaris A S. Expression analysis of defense-related genes in cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum infection and following chemical elicitation using a salicylic acid analog and methyl jasmonate. Plant Molecular Biology Reporter, 2012, 30: 225-234.

[14]Roelfs A P. Race specificity and method of study//Bushnell W R, Roelfs A P. The Cereal Rusts Volume I: Origins, Specificity, Structure and Physiology. Academic Press, Inc, 1984.

[15]雷杨, 张志毅, 刘文凤, 王兴, 安新民, 林善枝, 郑会全. 3个毛白杨病程相关蛋白基因的克隆及表达. 东北林业大学学报, 2012, 40(6): 67-75.

Lei Y, Zhang Z Y, Liu W F, Wang X, An X M, Lin S Z, Zheng H Q. Cloning and expression of three pathogenesis related protein genes from Populus tomeutosa. Journal of Northeast Agricultural University, 2012, 40(6): 67-75. (in Chinese)

[16]Kyte J, Doolittle R F. A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology, 1982, 157(6): 105-132.

[17]Nielsen H, Engelbrecht J, Brunak S, von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering, 1997, 10(1): 1-6

[18]Seyfarth R, Feuillet C, Schachermayr G, Winzeler M, Keller B. Development of a molecular marker for the adult plant leaf rust resistance gene Lr35 in wheat. Theoretical and Applied Genetics, 1999, 99: 554-560.

[19]Chittoor J M, Leach J E, White F F. Differential induction of a peroxidase gene family during infection of rice by Xanthomonas oryzae pv. oryzae. Molecular Plant-Microbe Interactions, 1997, 10(7): 861-871.

[20]Hou M M, Li W J, Bai H, Liu Y M, Li L Y, Liu L J, Liu B, Liu G Z. Characteristic expression of rice pathogenesis-related proteins in rice leaves during interactions with Xanthomonas oryzae pv. oryzae. Plant Cell Report, 2012, 31: 895-904.

[21]Kim S T, Yu S, Kang Y H. The rice pathogen-related protein 10 (Jiosprl0) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Report, 2008, 27(3): 593-603.

[22]Nakazaki T, Tsukiyama T, Okumoto Y, Kageyama D, Naito K, Inouye K, Tanisaka T. Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.). Genome, 2006, 49: 619-630.

[23]张计育, 渠慎春, 薛华柏, 高志红, 郭忠仁, 章镇. 湖北海棠病程相关蛋白MhPR8基因的克隆与表达. 中国农业科学, 2012, 45(8): 1568-1575.

Zhang J Y, Qu S C, Xue H B, Gao Z H, Guo Z R, Zhang Z. Isolation and expression of pathogenesis-related protein gene MhPR8 from Malus hupehensis. Scientia Agricultura Sinica, 2012, 45(8): 1568-1575. (in Chinese)

[24]Wan L L, Zha W J, Cheng X Y, Liu L, Liu C X, Wang Z Q, Du B, Chen R Z, Zhu L L, He G C. A rice β-1,3-glucanase gene Osg1 is required for callose degradation and pollen development. Planta, 2011, 233: 309-323.

[25]Von Dahl C C, Baldwin I T. Deciphering the role of ethylene in plant herbivore interactions. Journal of Plant Growth Regulation, 2007, 26: 201-209.

[26]Vlot A C, Klessig D F, Park S W. Systemic acquired resistance: the elusive signal(s). Current Opinion in Plant Biology, 2008, 11: 436-442.

[27]Glazebrook J. Genes controlling expression of defense responses in Arabidopsis-2001 status. Current Opinion in Plant Biology, 2001, 4: 301-308.

[28]Tang Y, Kuang J F, Wang F Y, Chen L, Hong K Q, Xiao Y Y, Xie H, Lu W J, Chen J Y . Molecular characterization of PR and WRKY genes during SA- and MeJA-induced resistance against Colletotrichum musae in banana fruit. Postharvest Biology and Technology, 2013, 79: 62-68.

[29]Berrocal-Lobo M, Molina A. Arabidopsis defense response against Fusarium oxysporum. Trends in Plant Science, 2008, 13(3): 145-150.

[30]Pandey S, Nelson D C, Assmann S M. Two novel GPCR-type G proteins are abscisic acid receptors in Arabidopsis. Cell, 2009, 136: 136-148.

[31]魏新燕, 杨文香, 刘大群, 孔俊英. 150 个小麦品种 (系) 抗叶锈基因Lr35分子检测. 中国农业科学, 2004, 37(12): 1951-1954.

Wei X Y, Yang W X, Liu D Q, Kong J Y. MAS for leaf rust resistance gene Lr35 in 150 wheat cultivars. Scientia Agricultura Sinica, 2004, 37(12): 1951-1954.
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