高温和外源ABA对不同持绿型小麦品种籽粒发育及内源激素含量的影响

doi:10.3864/j.issn.0578-1752.2014.11.005

Abstract

Abstract: 【Objective】 The purpose of this study was to test the effects of high temperature stress and spraying exogenous absisic acid (ABA) post-anthesis on endosperm cell division, grain filling and changes of endogenous hormones in different types of stay-green wheat cultivars, and provide a theoretical basis for enhancing grain weight and minimizing the loss of grain yield result from high temperature. 【Method】 Both Wennong 6 (a stay-green cultivar) and Jimai 20(a non-stay-green cultivar) were grown in the experimental plots covered with transparent plastic sheds at 1 to 5 days after anthesis (DAA), which were mimicked the high temperature that occurs during the natural heat event in this region, whilst exogenous ABA (10 mg?L-1) to the spikes was sprayed at the rate of 100 mL?m-2 between 5 pm and 6 pm from 1 to 3 DAA. High performance liquid chromatography method (HPLC) and a simple method of endosperm cell number determination were used to determine endogenous hormones contents and endosperm cell number, respectively. Both grain filling process and endosperm cell division process were simulated by Richard equation, and then the related parameters were calculated. 【Result】 The endosperm cell number of superior and inferior grains in both cultivars were significantly decreased by high temperature stress, and the endosperm cell division rate was markedly decreased. However, high temperature extended the active endosperm cell division period and the division duration. The grain filling rate of inferior grains in both cultivars was decreased in the high temperature treatment. Grain-filling duration and the active growth phase were also reduced in the high temperature treatment. High temperature after anthesis significantly decreased the number of grains per spike and thousand-grain weight. In contrast, Wennong 6 showed 3.7 and 8.2 grain/spike reduction in superior and inferior grains, respectively. Similarly, Jimai 20 was reduced by 1.3 and 4.3 grain/spike in superior and inferior grains, respectively. The final grain yield of Jimai 20 decreased by 26.22% due to high temperature treatment, and that of Wennong6 decreased only by 19.65%. Spraying exogenous ABA obviously improved grain filling rate, endosperm division rate and increased endosperm cell number in both normal and high temperature treatments. Although the zeatin riboside (ZR) contents in superior and inferior grains were significantly decreased in the high temperature treatment, the gibberellin (GA3) contents in Jimai 20 were markedly enhanced at 3 to 27 DAA, and those in Wennong 6 were also increased at 12 to 27 DAA. The auxin (IAA) content of inferior grains in Wennong 6 was significantly decreased at 15 to 27 DAA in high temperature treatment. The ZR content of superior grains in Jimai 20 was decreased at 3 to 9 DAA by spraying exogenous ABA in the high temperature treatment, but endogenous ABA content of superior grains in Jimai 20 was obviously increased at 3 to 28 DAA and that in Wennong 6 was also increased at 3 to 18 DAA. In the normal temperature treatment, spraying ABA significantly decreased the GA3 contents in both Wennong 6 and Jimai 20. Together, spraying exogenous ABA decreased the GA3 contents of superior and inferior grains in Wennng 6 at 3 to 27 DAA in high temperature treatment, and decreased those in Jimai 20 at 3 to12 DAA and 6 to 15 DAA, respectively. In the normal temperature treatment spraying ABA increased IAA content of superior grains in Jimai 20 at 12 to 18 DAA, and also increased that in Wennong 6 at 6 to 18 DAA. Meanwhile, IAA contents of inferior grains in both cultivars were significantly improved at 6 to 27 DAA. In addition, compared with Jimai 20, Wennong 6 had longer grain-filling duration and the active growth phase, higher mean grain-filling rate and maximum grain-filling rate, larger thousand-grain weight, and greater grain yield.【Conclusion】Both no-stay-green cultivar and inferior grains of the two cultivars were much more influenced by high temperature stress. Grain weight and yield reduction result from high temperature decreased kernel sink and grain filling rate. Exogenous ABA improved grain yield due to increasing the grain sink capacity and grain filling rate through regulating endogenous hormones contents to promote endosperm division and photosynthate accumulation in normal treatment and high temperature stress.

Key words: high temperature , endogenous hormones , stay-green , endosperm cell number , grain filling

YANG Dong-Qing-1, WANG Zhen-Lin-1, NI Ying-Li-1, 2 , YIN Yan-Ping-1, CAI Tie-1, YANG Wei-Bing-1, PENG Dian-Liang-1, CUI Zheng-Yong-1, JIANG Wen-Wen-1. Effect of High Temperature Stress and Spraying Exogenous ABA Post-Anthesis on Grain Filling and Grain Yield in Different Types of Stay-Green Wheat[J].Scientia Agricultura Sinica, 2014, 47(11): 2109-2125.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2014.11.005

https://www.chinaagrisci.com/EN/Y2014/V47/I11/2109

References

[1]闫素辉, 尹燕枰, 李文阳, 梁太波, 李勇, 邬云海, 王平, 耿庆辉, 戴忠民, 王振林. 灌浆期高温对小麦籽粒淀粉的积累、粒度分布及相关酶活性的影响. 作物学报, 2008, 34(6): 1092-1096.

Yan S H, Yin Y P, Li W Y, Liang T B, Li Y, Wu Y H, Wang P, Geng Q H, Dai Z M, Wang Z L. Effect of high temperature during grain filling on starch accumulation, starch granule distribution, and activities of related enzymes in wheat grains. Acta Agronomica Sinica, 2008, 34(6): 1092-1096. (in Chinese)

[2]邓振镛, 张强, 徐金芳, 陈敏, 秦三杰, 张树誉. 高温热浪与干热风的危害特征比较研究. 地球科学进展, 2009, 24(8): 865-873.

Deng Z Y, Zhang Q, Xu J F, Chen M, Qin S J, Zhang S Y. Comparative studies of the harm characteristic of hot-dry wind and high temperature heat waves. Advances in Earth Science, 2009, 24(8): 865-873. (in Chinese)

[3]Prasad P V V, Pisipati S R, Momcilovic I, Ristic Z. Independent and combined effects of high temperature and drought stress during grain filling on plant yield and chloroplast EF-Tu expression in spring wheat. Journal of Agronomy and Crop Science, 2011, 197(6): 430-441.

[4]Ferris R, Ellis R H, Wheeler T R, Hadley P. Effect of high temperature stress at anthesis on grain yield and biomass of field-grown crops of wheat. Annals of Botany, 1998, 82: 631-639.

[5]Hays D B, Do J H, Mason R E, Morgan G, Finlayson S A. Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar. Plant Science, 2007, 172: 1113-1123.

[6]马启林, 李阳生, 田小海, 鄢圣之, 雷慰慈, 中田升. 高温胁迫对水稻贮藏蛋白质的组成和积累形态的影响. 中国农业科学, 2009, 42(2): 714-718.

Ma Q L, Li Y S, Tian X H, Yan S Z, Lei W C, Zhong T S. Indluence of high temperature stress on composition and accumulation configuration of storage protein in rice. Scientia Agricultura Sinica, 2009, 42(2): 714-718. (in Chinese)

[7]石慧清, 龚月桦, 张东武. 花后高温对持绿型小麦叶片衰老及籽粒淀粉合成相关酶的影响. 植物生态学报, 2011, 35(7): 769-778.

Shi H Q, Gong Y H, Zhang D W. Effect of high temperature on leaf senescence and related enzymes of grain starch synthesis in stay-green wheat after anthesis. Chinese Journal of Plant Ecology, 2011, 35(7): 769-778. (in Chinese)

[8]马有宁, 陈铭学. 植物内源激素预处理方法与色谱检测技术的研究进展. 中国农学通报, 2011, 27(3): 15-19.

Ma Y N, Chen M X. Recent development in pretreatment and determination methods of plant endohormones. Chinese Agricultural Science Bulletin, 2011, 27(3): 15-19. (in Chinese)

[9]杨建昌. 水稻弱势粒灌浆机理与调控途径. 作物学报, 2010, 36(12): 2011-2019.

Yang J C. Mechanism and regulation in filling of inferior spikelets of rice. Acta Agronomica Sinica, 2010, 36(12): 2011-2019. (in Chinese)

[10]Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Liu L J. Activities of fructan- and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain filling. Planta, 2004, 220: 331-343.

[11]杨卫兵, 王振林, 尹燕枰, 李文阳, 李勇, 陈晓光, 王平, 陈二影, 郭俊祥, 蔡铁, 倪英丽. 外源ABA和GA对小麦籽粒内源激素含量及其灌浆进程的影响. 中国农业科学, 2011, 44(13): 2673-2682.

Yang W B, Wang Z L, Yin Y P, Li W Y, Li Y, Chen X G, Wang P, Chen E Y, Guo J X, Cai T, Ni Y L. Effects of spraying exogenous ABA or GA on the endogenous hormones concentration and filling of wheat grains. Scientia Agricultura Sinica, 2011, 44(13): 2673-2682. (in Chinese)

[12]王志琴, 叶玉秀, 杨建昌, 袁莉民, 王学明, 朱庆森. 水稻灌浆期籽粒中蔗糖合成酶活性的变化与调节. 作物学报, 2004, 30(7): 634-643.

Wang Z Q, Ye Y X, Yang J C, Yuan L M, Wang X M, Zhu Q S. Changes and regulations of sucrose synthase activity in rice grains during grain filling. Acta Agronomica Sinica, 2004, 30(7): 634-643. (in Chinese)

[13]Spano G, Di F N, Perrotta C, Platani C, Ronga G, Lawlor D W, Napier J A, Shewry P R. Physiological characterization of ‘stay green’ mutants in durum wheat. Journal of Experimental Botany, 2003, 54(386): 1415-1420.

[14]何萍, 金继运. 保绿型玉米的营养生理研究进展. 玉米科学, 2000, 8(4): 41-44.

He P, Jin J Y. Advances in physiological basis of maize that stay-green. Journal of Maize Sciences, 2000, 8(4): 41-44. (in Chinese)

[15]Thomas H, Howarth C J. Five ways to stay green. Journal of Experimental Botany, 2000, 51: 329-337.

[16]闫素辉, 尹燕枰, 李文阳, 李勇, 梁太波, 邬云海, 耿庆辉, 王振林. 花后高温对不同耐热性小麦品种籽粒淀粉形成的影响. 生态学报, 2008, 12(28): 6138-6147.

Yan S H, Yin Y P, Li W Y, Li Y, Liang T B, Wu Y H, Geng Q H, Wang Z L. Effect of high temperature after anthesis on starch formation of two wheat cultivars differing in heat tolerance. Acta Ecologica Sinica, 2008, 12(28): 6138-6147. (in Chinese)

[17]Xu X L, Wang Z M, Zhang J P. Effect of heat stress on photosynthetic characteristics of different green organs of winter wheat during grain-filling stage. Acta Botany Sinica, 2001, 43(6): 571-577.

[18]朱庆森, 曹显祖, 骆亦其. 水稻籽粒灌浆的生长分析. 作物学报, 1988, 14(3): 182-192.

Zhu Q S, Cao X Z, Luo Y Q. Growth analysis on the process of grain filling in rice. Acta Agronomy Sinica, 1988, 14(3): 182-192. (in Chinese)

[19]郭文善, 施劲松, 周振兴, 彭永欣. 小麦胚乳细胞简易计数方法. 江苏农学院学报, 1996, 17(1): 66-72.

Guo W S, Shi J S, Zhou Z X, Peng Y X. A simple method of endosperm cell number determination. Journal of Jiangsu Agricultural College, 1996, 17(1): 66-72. (in Chinese)

[20]Wheeler T R, Hong T D, Ellis R H, Batts G R, Morison J I L, Hadley P. The duration and rate of grain growth, and harvest index, of wheat (Triticum aestivum L.) in response to temperature and CO2. Journal of Experimental Botany, 1996, 47(298): 623-630.

[21]李永攀, 罗培高, 任正隆. 小麦持绿性及其与产量关系研究. 西南农业学报, 2008, 21(5): 1221-1225.

Li Y P, Luo P G, Ren Z L. Studies on the relation between the yield and trait of green-keeping wheat. Southwest China Journal of Agricultural Sciences, 2008, 21(5): 1221-1225. (in Chinese)

[22]史瑞青, 谢惠玲, 李鹏坤, 张慧琴. 花后喷施外源物质对小麦激素含量及源库的调节效应. 河南农业大学学报, 2006, 40(2): 122-126.

Shi R Q, Xie H L, Li P K, Zhang H Q. Effects of spraying exterior substance after florescence on wheat hormone contents and source-sink modulation. Journal of Henan Agricultural University, 2006, 40(2): 122-126. (in Chinese)

[23]Wang F, Cheng F M, Zhang G P. The relationship between grain filling and hormone content as affected by genotype and source–sink relation. Plant Growth Regulation, 2006, 49: 1-8.

[24]郑莎莎, 孙传范, 孙红春, 刘连涛, 赵金峰, 李存东. 不同外源激素对花铃期棉花主茎叶生理特性的影响. 中国农业科学, 2009, 42(12): 4383-4389.

Zheng S S, Sun C F, Sun H C, Liu L T, Zhao J F, Li C D. Effects of different exogenous hormones on physiological characteristics of main stem leaves at flower and boll stage in cotton. Scientia Agricultura Sinica, 2009, 42(12): 4383-4389. (in Chinese)

[25]Yang J C, Zhang J H, Huang Z L, Wang Z Q, Zhu Q S, Liu L J. Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice. Annals of Botany, 2002, 90: 369-377.

[26]徐云姬, 顾道健, 张博博, 张耗, 王志琴, 杨建昌. 玉米果穗不同部位籽粒激素含量及其与胚乳发育和籽粒灌浆的关系. 作物学报, 2013, 39(8): 1452-1461.

Xu Y J, Gu D J, Zhang B B, Zhang H, Wang Z Q, Yang J C. Hormone contents in kernels at different positions on an ear and their relationship with endosperm development and kernel filling in maize. Acta Agronomy Sinica, 2013, 39(8): 1452-1461. (in Chinese)

[27]刘霞, 穆春华, 尹燕枰, 姜春明, 王振林. 花后高温、弱光及其双重胁迫对小麦籽粒内源激素含量与增重进程的影响. 作物学报, 2007, 33(4): 677-681.

Liu X, Mu C H, Yin Y P, Jiang C M, Wang Z L. Effects of high temperature and shading stress after anthesis on endogenous hormone contents and filling process in wheat grain. Acta Agronomica Sinica, 2007, 33(4): 677-681. (in Chinese)

[28]Yang J C, Zhang J H, Wang Z Q, Zhu Q S, Wang W. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology, 2001, 127: 315-323.

[29]闫洁, 曹连莆, 张薇, 陈季恂, 吴学川. 土壤水分胁迫对大麦籽粒内源激素及灌浆特性的影响. 石河子大学学报, 2005, 23(1): 30-38.

Yan J, Cao L P, Zhang W, Chen J X, Wu X C. Effected on endogenous hormone and grain filing characteristic under soil water stress. Journal of Shihezi University, 2005, 23(1): 30-38. (in Chinese)

[30]崔志青, 尹燕枰, 田奇卓, 王广昌, 孟范玉, 王平, 王振林. 喷施ABA 对两个穗型不同小麦穗颈节伤流、穗部性状及产量的影响. 生态学报, 2011, 31(4): 1085-1092.

Cui Z Q, Yin Y P, Tian Q Z, Wang G C, Meng F Y, Wang P, Wang Z L. Effects of spraying ABA on bleeding intensity in neck-panicle node,

spike traits and grain yields of two different panicle-type winter wheat. Acta Ecologica Sinica, 2011, 31(4): 1085-1092. (in Chinese)

[31]夏国海, 张大鹏, 贾文锁. IAA、GA和ABA对葡萄果实14C蔗糖输入与代谢的调控. 园艺学报, 2000, 27(1): 6-10.

Xia G H, Zhang D P, Jia W S. Effects of IAA, GA and ABA on 14C-sucrose import and metabolism in grape berries. Acta Horticulture Sinica, 2000, 27(1): 6-10. (in Chinese)

[32]姜华, 高晓玲, 万佳, 陈静, 王平荣, 汪旭东, 徐正君. 脱落酸对水稻愈伤组织糖类代谢及相关酶活性的影响. 中国水稻科学, 2006, 20(4): 406-410.

Jiang H, Gao X L, Wan J, Chen J, Wang P R, Wang X D, Xu Z J. Effect of abscisic acid on saccharide metabolism and related enzyme activities in rice callus. Journal of Rice Science, 2006, 20(4): 406-410. (in Chinese)

[33]杨建昌, 王志琴, 朱庆森, 苏宝林. ABA和GA对水稻籽粒灌浆过程的调控. 作物学报, 1999, 25(3): 341-348.

Yang J C, Wang Z Q, Zhu Q S, Su B L. Regulation of ABA and GA to the grain filling of rice. Acta Agronomica Sinica, 1999, 25(3): 341-348. (in Chinese)

[34]Calderini D F, Abeledo L G, Savin R, Slafer G A. Final grain weight in wheat as affected by short periods of high temperature during pre- and post-anthesis under field conditions. Australian Journal of Plant Physiology, 1999, 26: 453-458.

[35]Calederini D F, Abeledo L G, Avin R S, Slafer G A. Effect of temperature and carpel size during pre-anthesis on potential grain weight in wheat. Journal of Agricultural Science, 1999, 132: 453-459.

[36]Banowetz G M, Ammar K, Chen D D. Postanthesis temperatures influence cttokinin accumulation and wheat kernel weight. Plant, Cell and Environment, 1999, 22: 309-316.

[37]Talukder A S M H M, Mcdonald G K, Gill G S. Effect of short-term heat stress prior to flowering and at early grain set on the utilization of water-soluble carbohydrate by wheat genotypes. Field Crops Research, 2013, 147: 1-11.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Effect of High Temperature Stress and Spraying Exogenous ABA Post-Anthesis on Grain Filling and Grain Yield in Different Types of Stay-Green Wheat

RichHTML

PDF

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	FENG XiangQian,YIN Min,WANG MengJia,MA HengYu,CHU Guang,LIU YuanHui,XU ChunMei,ZHANG XiuFu,ZHANG YunBo,WANG DanYing,CHEN Song. Effects of Meteorological Factors on Quality of Late Japonica Rice During Late Season Grain Filling Stage Under ‘Early Indica and Late Japonica’ Cultivation Pattern in Southern China [J]. Scientia Agricultura Sinica, 2023, 56(1): 46-63.
[2]	LI YiLing,PENG XiHong,CHEN Ping,DU Qing,REN JunBo,YANG XueLi,LEI Lu,YONG TaiWen,YANG WenYu. Effects of Reducing Nitrogen Application on Leaf Stay-Green, Photosynthetic Characteristics and System Yield in Maize-Soybean Relay Strip Intercropping [J]. Scientia Agricultura Sinica, 2022, 55(9): 1749-1762.
[3]	YU QiLong,HAN YingYan,HAO JingHong,QIN XiaoXiao,LIU ChaoJie,FAN ShuangXi. Effect of Exogenous Spermidine on Nitrogen Metabolism of Lettuce Under High-Temperature Stress [J]. Scientia Agricultura Sinica, 2022, 55(7): 1399-1410.
[4]	YIN YanYu,XING YuTong,WU TianFan,WANG LiYan,ZHAO ZiXu,HU TianRan,CHEN Yuan,CHEN Yuan,CHEN DeHua,ZHANG Xiang. Cry1Ac Protein Content Responses to Alternating High Temperature Regime and Drought and Its Physiological Mechanism in Bt Cotton [J]. Scientia Agricultura Sinica, 2022, 55(23): 4614-4625.
[5]	SHA YueXia, HUANG ZeYang, MA Rui. Control Efficacy of Pseudomonas alcaliphila Strain Ej2 Against Rice Blast and Its Effect on Endogenous Hormones in Rice [J]. Scientia Agricultura Sinica, 2022, 55(2): 320-328.
[6]	ZHANG Chuan,LIU Dong,WANG HongZhang,REN Hao,ZHAO Bin,ZHANG JiWang,REN BaiZhao,LIU CunHui,LIU Peng. Effects of High Temperature Stress in Different Periods on Dry Matter Production and Grain Yield of Summer Maize [J]. Scientia Agricultura Sinica, 2022, 55(19): 3710-3722.
[7]	XiaoFan LI,JingYi SHAO,WeiZhen YU,Peng LIU,Bin ZHAO,JiWang ZHANG,BaiZhao REN. Combined Effects of High Temperature and Drought on Yield and Photosynthetic Characteristics of Summer Maize [J]. Scientia Agricultura Sinica, 2022, 55(18): 3516-3529.
[8]	CHANG LiGuo,HE KunHui,LIU JianChao. Mining of Genetic Locus of Maize Stay-Green Related Traits Under Multi-Environments [J]. Scientia Agricultura Sinica, 2022, 55(16): 3071-3081.
[9]	XIAO ShanShan, ZHANG YiFei, YANG KeJun, MING LiWei, DU JiaRui, XU RongQiong, SUN YiShan, LI WeiQing, LI GuiBin, LI ZeSong, LI JiaYu. Effects of Intercropping with Different Maturity Varieties on Grain Filling, Dehydration Characteristics and Yield of Spring Maize [J]. Scientia Agricultura Sinica, 2022, 55(12): 2294-2310.
[10]	HU DanDan,LI RongFa,LIU Peng,DONG ShuTing,ZHAO Bin,ZHANG JiWang,REN BaiZhao. Mixed-Cropping Improved on Grain Filling Characteristics and Yield of Maize Under High Planting Densities [J]. Scientia Agricultura Sinica, 2021, 54(9): 1856-1868.
[11]	HAN ZhanYu,WU ChunYan,XU YanQiu,HUANG FuDeng,XIONG YiQin,GUAN XianYue,ZHOU LuJian,PAN Gang,CHENG FangMin. Effects of High-Temperature at Filling Stage on Grain Storage Protein Accumulation and Its Biosynthesis Metabolism for Rice Plants Under Different Nitrogen Application Levels [J]. Scientia Agricultura Sinica, 2021, 54(7): 1439-1454.
[12]	ZHANG MingJing,HAN Xiao,HU Xue,ZANG Qian,XU Ke,JIANG Min,ZHUANG HengYang,HUANG LiFen. Effects of Elevated Temperature on Rice Yield and Assimilate Translocation Under Different Planting Patterns [J]. Scientia Agricultura Sinica, 2021, 54(7): 1537-1552.
[13]	WANG JunJie,TIAN Xiang,QIN HuiBin,WANG HaiGang,CAO XiaoNing,CHEN Ling,LIU SiChen,QIAO ZhiJun. Regulation Effects of Photoperiod on Growth and Leaf Endogenous Hormones in Broomcorn Millet [J]. Scientia Agricultura Sinica, 2021, 54(2): 286-295.
[14]	YAN ZhenHua,LIU DongYao,JIA XuCun,YANG Qin,CHEN YiBo,DONG PengFei,WANG Qun. Maize Tassel Development, Physiological Traits and Yield Under Heat and Drought Stress During Flowering Stage [J]. Scientia Agricultura Sinica, 2021, 54(17): 3592-3608.
[15]	SHAO JingYi,LI XiaoFan,YU WeiZhen,LIU Peng,ZHAO Bin,ZHANG JiWang,REN BaiZhao. Combined Effects of High Temperature and Drought on Yield and Stem Microstructure of Summer Maize [J]. Scientia Agricultura Sinica, 2021, 54(17): 3623-3631.