Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (12): 2501-2513.doi: 10.3864/j.issn.0578-1752.2013.12.011

• HORTICULTURE • Previous Articles     Next Articles

Identification, Evolution and Expression Analysis of the LBD Gene Family in Tomato

 WANG  Xiao-Fei, LIU  Xin, SU  Ling, SUN  Yong-Jiang, ZHANG  Shi-Zhong, HAO  Yu-Jin, YOU  Chun-Xiang   

  1. State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology , College of Horticultural Science and Engineering, Shandong Agricultural University, Taian 271018, Shandong
  • Received:2012-12-25 Online:2013-06-15 Published:2013-02-26

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Abstract: 【Objective】Identification of LBD genes from tomato genome, and analysis of phylogeny，gene structure, chromosome location, phylogenetic and tissue expression pattern analysis of LBD family genes in tomato will be useful to the functions identification of plant LBD genes.【Method】Based on tomato genome database and bioinformatic method, tomato LBD family genes were identified and the genes were sequenced. A phylogenetic tree was created using the MEGA5 program. Gene structure and chromosomes location were done by Perl-based program, MapDraw and GSDS. Expression pattern of LBD genes at different development stages was analyzed based on the existing microarray database.【Result】A total of 46 LBD genes were systematically identified from tomato and classified into 2 classes (class I and class II), then was classified into 5 subfamilies (Ia, Ib, Ic, Id and II) according to the gene structure and conserved domain phylogeny relationship. They were distributed on 10 of tomato chromosomes, suggesting that they have an extensive distribution on the tomato chromosomes. Most of the LBD genes had differential expression pattern and response to external stimulation. 【Conclusion】Forty-six LBD family genes in tomato were identified by genome-wide screening. They were classified into 2 classes, 5 subfamilies and distributed on 10 chromosomes with different expression patterns in different tissues and developmental stages. They had special response to external stimulation. These results are helpful for the functional analysis of LBD genes in plants.

Key words: tomato , LBD gene family , phylogeny analysis , gene expression

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