Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 830-840.doi: 10.3864/j.issn.0578-1752.2013.04.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning and Expression Pattern of Duck Serum Albumin Gene During Viral Infection

 XU  Qi, CHEN  Yang, LI  Xiu, HUANG  Zheng-Yang, ZHANG  Yang, LI  Xin-Yu, TONG  Yi-Yu, DUAN  Xiu-Jun, CHEN  Guo-Hong   

  1. 1.Jiangsu Key Laboratory of Animal Genetics, Breeding and Molecular Design, Yangzhou 225009, Jiangsu;
    2.School of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045;
    3.National Waterfowl Germplasm Resorurse,    Taizhou 225300, Jiangsu
  • Received:2012-07-06 Online:2013-02-15 Published:2012-09-05

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Abstract: 【Objective】This experiment was conducted to clone serum albumin (ALB) gene and explore albumin gene molecular structure and expression regularity in duck. 【Method】Screening albumin as a candidate gene based on the results of suppression subtractive hybridization (SSH). The disease models infected duckling virus hepatitis and polyriboinosinic polyribocytidylic acid, Poly(I:C) were constructed. The cDNA and 5′ flanking sequence of ALB were obtained by RT-PCR, RACE and genome walking, and then the sequences were analyzed by bioinformatics approaches. The temporal spatial expression of ALB in tissues was detected by RT-qPCR. The results showed that the full-length cDNA of ALB gene was 2 107 bp, including 47 bp of 5′UTR, 212 bp of 3′UTR, and 1 848 bp of open reading frame (ORF). And the potential transcriptional factor binding sites were predicted in 5′ flanking region including TAAT box, CAAT box, HSF, HNF, C/EBP and so on in liver. The results of RT-qPCR showed that ALB mRNA was specifically expressed in liver, the ALB mRNA levels of livers decreased first, then increased and remained stable 24 hours late in the 3-day-old ducklings treated with duckling virus hepatitis and Poly(I:C). 【Conclusion】The cDNA and 5′ flanking sequence of ALB were detected successfully in duck, its genetic relationship with other kinds of flow were closing by constructing phylogenetic tree. The albumin gene expression regularity was discovered after infected duckling virus hepatitis and Poly(I:C).

Key words: duck , albumin gene , molecular cloning , gene expression

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