Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (4): 830-840.doi: 10.3864/j.issn.0578-1752.2013.04.017

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Molecular Cloning and Expression Pattern of Duck Serum Albumin Gene During Viral Infection

 XU  Qi, CHEN  Yang, LI  Xiu, HUANG  Zheng-Yang, ZHANG  Yang, LI  Xin-Yu, TONG  Yi-Yu, DUAN  Xiu-Jun, CHEN  Guo-Hong   

  1. 1.Jiangsu Key Laboratory of Animal Genetics, Breeding and Molecular Design, Yangzhou 225009, Jiangsu;
    2.School of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045;
    3.National Waterfowl Germplasm Resorurse,    Taizhou 225300, Jiangsu
  • Received:2012-07-06 Online:2013-02-15 Published:2012-09-05

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Abstract: 【Objective】This experiment was conducted to clone serum albumin (ALB) gene and explore albumin gene molecular structure and expression regularity in duck. 【Method】Screening albumin as a candidate gene based on the results of suppression subtractive hybridization (SSH). The disease models infected duckling virus hepatitis and polyriboinosinic polyribocytidylic acid, Poly(I:C) were constructed. The cDNA and 5′ flanking sequence of ALB were obtained by RT-PCR, RACE and genome walking, and then the sequences were analyzed by bioinformatics approaches. The temporal spatial expression of ALB in tissues was detected by RT-qPCR. The results showed that the full-length cDNA of ALB gene was 2 107 bp, including 47 bp of 5′UTR, 212 bp of 3′UTR, and 1 848 bp of open reading frame (ORF). And the potential transcriptional factor binding sites were predicted in 5′ flanking region including TAAT box, CAAT box, HSF, HNF, C/EBP and so on in liver. The results of RT-qPCR showed that ALB mRNA was specifically expressed in liver, the ALB mRNA levels of livers decreased first, then increased and remained stable 24 hours late in the 3-day-old ducklings treated with duckling virus hepatitis and Poly(I:C). 【Conclusion】The cDNA and 5′ flanking sequence of ALB were detected successfully in duck, its genetic relationship with other kinds of flow were closing by constructing phylogenetic tree. The albumin gene expression regularity was discovered after infected duckling virus hepatitis and Poly(I:C).

Key words: duck , albumin gene , molecular cloning , gene expression

[1]Schmilovitz-Weiss H, Tovar A, Halpern M, Sulkes J, Braun M, Rotman Y, Tur-Kaspa R, Ben-Ari Z. Predictive value of serum globulin levels for the extent of hepatic fibrosis in patients with chronic hepatitis B infection. Journal of Viral Hepatitis, 2006, 13(10): 671-677.

[2]Kim Y H, Lee Y W, Jeon B R, Lee Y K, Shin H B, Kang D H, Park S K, Hong D S, Lee S T, Kim J W, Ki C S. Clinical characteristics and ALB gene mutation analysis of Korean patients with bisalbuminemia. The Korean Journal Laboratory Medicine, 2010, 30(3): 307-311.

[3]Dagnino M, Caridi G, Aydin Z, Ozturk S, Karaali Z, Kazancioglu R, Cefle K, Gursu M, Campagnoli M, Galliano M, Minchiotti L. A novel frameshift deletion in the albumin gene causes analbuminemia in a young Turkish woman. Clinica Chimica Acta, 2010, 411(21/22): 1711-1715.

[4]Huang Y H, Li N, Burt D W, Wu F. Genomic research and applications in theduck (Anas platyrhynchos). World’s Poultry Science Journal, 2008, 64: 329-341.

[5]Izban M G, Papaconstantinou J. Cell-specific expression of mouse albumin promoter. Evidence for cell-specific DNA elements within the proximal promoter region and cis-acting DNA elements upstream of-160. Journal of Biological Chemistry, 1989(16): 9171-9179.

[6]徐  琳, 徐评议, 梁秀龄. 小鼠白蛋白基因的特异性调控及其在转基因动物中的应用. 国外医学遗传学分册, 2003, 26(5): 287-290.

Xu L, Xu P Y, Liang X L. Cell-specific regulation of mouse albumin gene and its application in transgenic animals. Section Genet Foreign Medical Sciences, 2003, 26(5): 287-290. (in Chinese)

[7]张  艳. 利用HSV-tk研制肝脏特异损伤的转基因小鼠模型研究[D]. 上海: 上海第二医科大学, 2004.

Zhang Y. Development of transgenic mouse model for liver-specific damage using HSV-tk[D]. Shanghai: Shanghai Second Medical University, 2004. (in Chinese)

[8]Ljubimova J Y, Petrovic L M, Wilson S E, Geller S A, Demetriou A A. Expression of HGF, its receptor c-met, c-myc, and albumin in cirrhotic and neoplastic human liver tissue. The Journal of Histochemistry and Cytochemistry, 1997, 45(1): 79-87.

[9]Daqnino M, Caridi G, Haenni U, Duss A, Areqqer F, Campaqnoli M, Galliano M, Minchiotti L. Molecular diagnosis of analbuminemia: a new case caused by a nonsense mutation in the albumin gene. International Journal of Molecular Sciences, 2011, 12(11): 7314-7322.

[10]Gianluca C, Monica D, Marco D D, Filiz A, Gungor B, Fatih B, Kadir D, Asli O, Suut G, Kivanc C, ?ukru O, Sukru P, Monica C, Monica G, Lorenzo M. A novel two bases deletion in the albumin gene causes analbuminaemia in a young Turkish man. Clinica Chimica Acta, 2012, 413(9/10): 950-951.

[11]Davis R L, Peters T Jr, Brennan S O. Novel frameshift in the serum albumin gene results in analbuminemia through premature truncation and post translational modification. Clinical Biochemistry, 2008, 41(18): 1482-1485.

[12]李  秀, 徐  琪, 张  扬, 毕瑜林, 赵荣雪, 陈昌义, 段修军, 陈国宏. 雏鸭肝炎病毒侵染下肝脏消减cDNA文库的构建及差异基因筛选. 畜牧兽医学报, 2012, 43(2): 211-219.

Li X, Xu Q, Zhang Y, Bi Y L, Zhao R X, Chen C Y, Duan X J, Chen G H. Construction of a suppression subtractive hybridization cDNA library to screen differentially expressed genes from duck liver infected duck hepatitis virus. Acta Veterinaria et Zootechnica Sinica, 2012, 43(2): 211-219. (in Chinese)

[13]Louise G D, Sham V N, Elizabeth M D. The marsupial CD8 gene locus: molecular cloning and expression analysis of the alpha and beta sequences in the gray short-tailed opossum (Monodelphis domestica) and the tammar wallaby (Macropus eugenii). Veterinary Immunology and Immunopathology, 2009, 129(1): 14-27.

[14]王  亮, 胡建平. 结核分枝杆菌(H37RV)分泌性蛋白的生物信息学预测方法. 第四军医大学学报, 2006, 27(1): 86-89.

Wang L, Hu J P. Bioinformatics prediction strategy for Mycobacterium tuberculosis(H37Rv) secreted proteins. The Journal of the Fourth Military Medical University, 2006, 27(1): 86-89. (in Chinese)

[15]李  虎, 贺小英, 杨瑞峰, 李向臣, 华  松, 张  涌. 人血清白蛋白基因的克隆及序列分析. 安徽农业科学, 2005, 33(5): 801-802.

Li H, He X Y, Yang R F, Li X C, Hua S, Zhang Y. Clone and sequence of human serum albumin cDNA from human placenta. Journal of Anhui Agricultural Sciences, 2005, 33(5): 801-802. (in Chinese)

[16]Robert J G, Robert W, Monika V, Rabindra N R, Peter C K, Roger G. D. The 5′ noncoding and flanking regions of the avian very low density ApolipoproteinⅡand serum albumin genes. The Journal of Biological Chemistry, 1983, 258(7): 4556-4564.

[17]Maire P, Wuarin J, Schibler U. The role of cis-acting promoter elements in tissue-specific albumin gene expression. Science, 244(4902): 343-346.

[18]Zaret K S, Liu J K, DiPersio M C. Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer. Proceeding of the National Academy of Science of the United States of America, 1990, 87(13): 5469-5473.

[19]Cirillo L A, Lin F R, Cuesta I, Friedman D, Jarnik M, Zaret K S. Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4. Molecular Cell, 2002, 9(2): 279-289.

[20]李  勃, 熊海燕, 陈红星, 邓继先. 猪血清白蛋白基因5′端调控区的克隆与分析. 广西师范大学学报: 自然科学版, 2009, 27(3): 58-61.

Li B, Xiong H Y, Chen H X, Deng J X. Cloning and motif analysis of psa 5′-terminal regulation region from porcine. Journal of Guangxi Normal University: Natural Science Edition, 2009, 27(3): 58-61. (in Chinese)

[21]李  袆, 王  葵. 热休克转录因子1在肝细胞癌中的表达及意义. 临床医药实践, 2009, 18(7): 490-493.

Li Y, Wang K. The study of expression of HSF1 primary hepatic carcinoma. Proceeding of Clinical Medicine, 2009, 18(7): 490-493. (in Chinese)

[22]Wu K J. Activation of albumin and other liver-specific gene expression in fibroblast-pancreatic cell hybrids: different roles of transcription factors. Experimental Cell Research, 1993, 208(1): 241-247.

[23]Levrat F, Vallet V, Berbar T, Miquerol L, Kahn A, Antoine B. Influence of the content in transcription factors on the phenotype of mouse hepatocyte-like cell lines (mhAT). Experimental Cell Research, 1993, 209(2): 307-316.

[24]Bhattacharyya S, Tian J, Bouhassira E E, Locker J. Systematic targeted Integration to study gene control elements. PLoS ONE, 2011, 6(8): 23234.

[25]Zhang Y, Huang S Z, Zeng Y T. The Effect of HSV-tk/GCV on hepatic specific damage driven by murine ALB promoter/enhancer. Acta Genetica Sinica, 2004, 31(10): 1053-1060.

[26]Herweijer H, Zhang G F, Subbotin V M, Budler V, Williams P, Wolff J A. Time course of gene expression after plasmid DNA gene transfer to the liver. The Journal of Gene Medicine, 2001, 3(3): 280-291.

[27]徐  琳, 梁秀龄, 徐评议. 肝脏特异性表达载体Kbpa-la/Alb- ATP7B的构建及表达. 中国病理生理杂志, 2006, 22(6): 1045-1048.

Xu L, Liang X L, Xu P Y. Construction and expression of mouse liver-specific expression vector Kb-pala/Alb-ATP7B. Chinese Journal of Pathophysiology, 2006, 22(6): 1045-1048. (in Chinese)

[28]崔治中, 崔保安. 兽医免疫学. 北京: 中国农业出版社, 2007: 120-129.

Cui Z Z, Cui B A. Veterinary Immunology. Beijing: China Agriculture Press, 2007: 120-129. (in Chinese)

[29]Woodworth C D, Isom H C. Regulation of albumin gene expression in a seriesof rat hepatocyte cell lines immortalized by simian virus 40 and maintainedin chemically defined medium. Molecular and Cellular Biology, 1987, 7(10): 3740-3748.

[30]Annoni G, Weiner F R, Colombo M, Czaja M J, Zern M A. Albumin and collagen gene regulation in alcohol- and virus-induced human liver disease. Gastroenterology, 1990, 98(1): 197-202.
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