Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (3): 623-629.doi: 10.3864/j.issn.0578-1752.2013.03.019

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Genetic Diversity and Phylogenetic Relationship Among Five Pony Populations of China

 XU  Long-Xin, YANG  Sheng-Lin, LI  Ai-Ping, NIE  Yu-Li, YANG  Hai-Bing, DAI  Yan   

  1. 1.Institute of Animal Husbandry and Veterinarian of Guizhou Province, Guiyang 550005
    2. Key Laboratory of Animal Genetic, Breeding and Reproduction for Mountainous Areas, Ministry of Education, Animal Science College of Guizhou University,   Guiyang 550025
    3.Station of Animal Husbandry and Veterinarian of Kaili, Kaili 560000, Guizhou
  • Received:2012-10-30 Online:2013-02-01 Published:2013-01-16

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Abstract: 【Objective】 In order to provide date and scientific basis for utilization and protection of biodiversity, diversity and phylogenetic relationships of five pony populations, 136 samples were studied. 【Method】 The PCR amplification and sequencing of mtDNA Cytb gene, the date analyzsis ways of biologic informatics were used. 【Result】 A total of 27 haplotypes were detected, and 40 polymorphic loci which account for 3.51% of the analyzed sites were found. The Yunnan and Guizhou populations had the highest haplotype diversity (Hd = 0.869) and nucleotide diversity (Pi = 0.0435), respectively, whereas the Yuexi and Ningqiang populations had the lowest respectively, but not far from each population. Each branch of the phylogenetic tree and NETwork results all had the information of five pony populations.【Conclusion】The five pony populations had higher genetic diversity in all, the Yunnan, Guizhou and Debao ponies had relatively higher genetic diversity, but Yuexi and Nongqiang pony were low. the phylogenetic research suggested that extensive gene flow exists among the five pony populations, and this study has provided evidence for Chinese ponies having multiple maternal origins.

Key words: Chinese pony , Cytb , genetic diversity , phylogenetic relationship

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