Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (3): 614-622.doi: 10.3864/j.issn.0578-1752.2013.03.018

• ANIMAL SCIENCE·VETERINARY SCIENCERE·SOURCE INSECT • Previous Articles     Next Articles

Promoter Characterization of Sheep Dlx3 Gene and Association of Promoter Polymorphisms with Wool Quality Traits in Chinese Merino

 PEI  Wen-Yu, YUN  Jie, RONG  En Guang, YANG  Hua, WANG  Zhi-Peng, WANG  Shou-Zhi, LI  Hui, WANG  Ning   

  1. 1.College of Animal Science and Technology, Northeast Agricultural University/Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture/Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin 150030
    2.Institute of Animal Husbandry and Veterinary, Xinjiang Academy of Agriculture and Reclamation Science, Shihezi 832000, Xinjiang
  • Received:2012-04-05 Online:2013-02-01 Published:2012-05-25

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Abstract: 【Objective】Dlx3 gene is an important candidate gene for wool quality in sheep, but its function and transcriptional regulation are not fully understood. The aim of the present study was to characterize sheep Dlx3 gene promoter and analyze the association of Dlx3 gene promoter polymorphisms with wool quality traits.【Method】 The 5′ flanking fragment of the sheep Dlx3 gene was amplified by PCR and cloned. Promoter activity was analyzed using the luciferase reporter gene system, and SNP was genotyped by PCR-RFLP. 【Result】Sequence analysis showed that the proximal promoter region of Dlx3 gene shared 23 transcription factor binding sites and a CpG island among human, mouse and sheep. Promoter activity analysis showed that sheep Dlx3 gene promoter was active in sheep embryonic fibroblasts. Promoter truncation mutation analysis showed that the -1 551 to -1 108 and -1 108 to -707 promoter regions had stronger promoter activity in vitro. Association analysis showed that SNP (G-1166A) was significantly associated with wool crimp.【Conclusion】Dlx3 gene promoter is active in sheep embryonic fibroblasts, and its proximal promoter region, not the distal promoter region, is conserved among human, mouse and sheep. The distal promoter region of Dlx3 gene has stronger promoter activity. The G-1166A is a potential marker of wool crimp for molecular marker-assisted selection in sheep.

Key words: sheep , Dlx3 gene , promoter activity , SNP , association analysis

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