Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (22): 4678-4687.doi: 0.3864/j.issn.0578-1752.2012.22.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Developmental Changes of Gene Expression of GHR and IGF-Ⅰ Genes and Their Association Analysis with Meat Quality Traits in Hu Sheep

 SUN  Wei, LI  Da, MA  Yue-Hui, GUAN  Wei-Jun, CHU  Ming-Xing, DING  Jia-Tong, LI  Bi-Chun, ZHANG  You-Fa, CHEN  Ling, WU  Wen-Zhong, ZHOU  Hong   

  1. 1.Animal Science and Technology College, Yangzhou University, Yangzhou 225009, Jiangsu
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193 
    3.Animal Science and Veterinary Medicine Bureau of Suzhou, Suzhou 215128, Jiangsu 
    4.Forestation, Herding, Fishing Bureau of Suining Country of Xuzhou, Suining 221200, Jiangsu
  • Received:2012-06-04 Online:2012-11-15 Published:2012-08-28

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Abstract: 【Objective】The objective of this study was to discuss the developmental changes of gene expression of growth hormone receptor (GHR) and insulin-like growth factor-Ⅰ(IGF-Ⅰ) and their association analysis with meat quality traits , and the relationship of the expression level between GHR gene and IGF-Ⅰgene in Hu sheep. 【Method】The developmental changes of gene expression of GHR gene and IGF-Ⅰgene on the early growth of Hu sheep were detected by real-time fluorescent quantitative RT-PCR. 【Result】The results were as followes: With increasing age after birth, the GHR expression trend of ewes was up-down-up-down-up, and 6-month reached the top. The GHR expression trend of rams was that 2-day-old to 2-month-old was increasing, 2-month-old to 4-month-old was decreasing, 4-month-old to 6-month-old was increasing and reached the top. With the increasing age after birth, the IGF-Ⅰgene expression trend of ewes was increasing from 2-day-old to 6-month-old which had the top expression. The IGF-Ⅰgene expression trend of rams was that at 2-day-old firstly increased to a peak at one-month-old, 1-month-old to 3-month-old had a decreasing process and reached the same level which was close to the newborn, and then increased with the increasing age, and 6-month-old reached the top. Most growth phases had a significant difference or extreme significant difference in GHR and IGF-Ⅰand the expressions of GHR and IGF-Ⅰbetween most rams and ewes that at the same growth phase had a significant difference or extreme significant difference. The results showed that the expressions of GHR and IGF-Ⅰhad a significant positive correlation, (0.01＜P＜0.05) the expressions of GHR and IGF-Ⅰgenes had a extreme significant positive correlation (P＜0.01) with diameter of muscle fibers and a extreme significant negative correlation (P＜0.01) with density of muscle fibers, and the expressions of GHR had a extreme significant positive correlation (P＜0.01) with shear force of muscle fibers, the expression of IGF-Ⅰhad a significant positive correlation with shear force of muscle fibers (0.01＜P＜0.05). 【Conclusion】The results showed that different stages and different genders had important influences on the expressions of GHR and IGF-Ⅰgenes in sheep skeletal muscle. After birth, the expressions of the two genes did not always increase or decrease, the turning points of different genes expression were not at the same time. The expression of GHR and IGF-Ⅰhad a significant positive correlation in early muscle traits of Hu sheep. GHR and IGF-Ⅰgene can be regarded as the candidate gene for the trait of meat quality in Hu Sheep.

Key words: Hu sheep , GHR gene , IGF-Ⅰgene , gene expression , meat quality traits

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