Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (14): 2838-2847.doi: 10.3864/j.issn.0578-1752.2012.14.006

• PLANT PROTECTION • Previous Articles     Next Articles

Identification of the Pathogen Causing Fusarium Root Rot of Pea and Diversity of Pathogenicity Genes

 XIANG  Ni, DUAN  Can-Xing, XIAO  Yan-Nong, WANG  Xiao-Ming, ZHU  Zhen-Dong   

  1. 1.中国农业科学院作物科学研究所/国家农作物基因资源与基因改良国家重大科学工程，北京 100081
    2.华中农业大学植物科技学院，武汉 430070
  • Received:2011-10-26 Online:2012-07-15 Published:2012-04-26

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Abstract: 【Objective】The objective of this study is to determine the pathogen causing Fusarium root rot on pea and diversity of pathogenicity genes.【Method】The Fusarium isolates were identified by PCR detection using specific primers for F. solani, morphological characteristics, and pathogenicity test. Pea pathogenicity genes PDA, PEP1, PEP3, and PEP5 in the isolates identified as F. solani f. sp. pisi were detected using the gene-specific primers.【Result】Ninety-six isolates were identified as F. solani f. sp. pisi. Pathogenicity tests showed that all isolates were pathogenic to pea variety “Caoyuan 27”. There was a great difference in virulence among the isolates with 8.3%, 10.4% and 81.3% being weakly virulent, moderately virulent and highly virulent to pea, respectively. Results of the PCR detection of the pathogenicity genes indicated that there were 10 combinations of pathogenicity genes (genotypes) in the 96 isolates and about 91% isolates possessed three or 4 pathogenicity genes. Most of the isolates containing four or three pathogenicity genes were highly virulent (87.4%) or moderately virulent (9.2%). The isolates without the PDA generally possessed weak virulence. These results suggest that virulence of F. solani f. sp. pisi isolates were determined by the type, the number, and/or the combination of the pathogenicity genes.【Conclusion】F. solani f. sp. pisi is the major pathogen causing pea root rot, and the highly virulent pathogen type appears to be prevalent in main pea production areas in China.

Key words: pea, Fusarium root rot, Fusarium solani f. sp. pisi, virulence, pathogenicity gene

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