Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2771-2778.doi: 10.3864/j.issn.0578-1752.2012.13.023

• RESEARCH NOTES • Previous Articles     Next Articles

The Mechanism of Red Coloring Difference Between Skin and Cortex in Malus sieversii f. neidzwetzkyana (Dieck) Langenf.

 WANG  Yan-Ling, ZHANG  Yan-Min, FENG  Shou-Qian, SONG  Yang, XU  Yu-Ting, ZHANG  You-Peng, CHEN  Xue-Sen   

  1. 1.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安 271018
    2.山东农业大学林学院，山东泰安 271018
    3.山东农业大学生命科学学院，山东泰安 271018
    4.山东农业大学科技处，山东泰安 271018
  • Received:2011-12-08 Online:2012-07-01 Published:2012-02-29

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Abstract: 【Objective】The aim of this study is to explore the red development mechanism by monitoring anthocyanin composition and content, transcriptional profile of anthocyanin related genes in Malus sieversii f. neidzwetzkyana (Dieck) Langenf.【Method】M. sieversii f. neidzwetzkyana (Dieck) Langenf was used as material to study anthocyanin composition of skin and cortex by HPLC, and to study the transcriptional profile of anthocyanin related genes by qPCR at four developmental stages. Anthocyanin contents in skin and cortex were determined at the four sampling times accordingly. 【Result】Cyanidin-3-galactoside was the main anthocyanin composition of skin and cortex, but anthocyanin content had different change trends at different developmental stages. The anthocyanin content of skin showed a declined pattern, while the anthocyanin content of cortex displayed an opposite tendency. Expression of the anthocyanin biosynthesis genes was correlated with anthocyanin content: expression of MsMYB10 in skin and cortex was very high, while expression of MsMYB10 showed an increasing pattern in skin and a ‘ high-low-high-low’ change trend in cortex during fruit development. 【Conclusion】The red coloring difference between skin and cortex of ‘Xiahongrou’ mainly relate to the anthocyanin content and the different expressions of anthocyanin gene. These results have provided a foundation for further exploring the red coloring mechanism and breeding cultivars of red-flesh apple.

Key words: Malus sieversii f. neidzwetzkyana, anthocyanins, MsMYB10, gene expression

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