Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (13): 2771-2778.doi: 10.3864/j.issn.0578-1752.2012.13.023

• RESEARCH NOTES • Previous Articles     Next Articles

The Mechanism of Red Coloring Difference Between Skin and Cortex in Malus sieversii f. neidzwetzkyana (Dieck) Langenf.

 WANG  Yan-Ling, ZHANG  Yan-Min, FENG  Shou-Qian, SONG  Yang, XU  Yu-Ting, ZHANG  You-Peng, CHEN  Xue-Sen   

  1. 1.山东农业大学园艺科学与工程学院/作物生物学国家重点实验室，山东泰安 271018
    2.山东农业大学林学院，山东泰安 271018
    3.山东农业大学生命科学学院，山东泰安 271018
    4.山东农业大学科技处，山东泰安 271018
  • Received:2011-12-08 Online:2012-07-01 Published:2012-02-29

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Abstract: 【Objective】The aim of this study is to explore the red development mechanism by monitoring anthocyanin composition and content, transcriptional profile of anthocyanin related genes in Malus sieversii f. neidzwetzkyana (Dieck) Langenf.【Method】M. sieversii f. neidzwetzkyana (Dieck) Langenf was used as material to study anthocyanin composition of skin and cortex by HPLC, and to study the transcriptional profile of anthocyanin related genes by qPCR at four developmental stages. Anthocyanin contents in skin and cortex were determined at the four sampling times accordingly. 【Result】Cyanidin-3-galactoside was the main anthocyanin composition of skin and cortex, but anthocyanin content had different change trends at different developmental stages. The anthocyanin content of skin showed a declined pattern, while the anthocyanin content of cortex displayed an opposite tendency. Expression of the anthocyanin biosynthesis genes was correlated with anthocyanin content: expression of MsMYB10 in skin and cortex was very high, while expression of MsMYB10 showed an increasing pattern in skin and a ‘ high-low-high-low’ change trend in cortex during fruit development. 【Conclusion】The red coloring difference between skin and cortex of ‘Xiahongrou’ mainly relate to the anthocyanin content and the different expressions of anthocyanin gene. These results have provided a foundation for further exploring the red coloring mechanism and breeding cultivars of red-flesh apple.

Key words: Malus sieversii f. neidzwetzkyana, anthocyanins, MsMYB10, gene expression

[1]Zhou Z Q, Li Y N. The RAPD evidence for the phylogenetic relationship of the closely related species of cultivated apple. Genetic Resources and Crop Evolution, 2000, 47: 353-357.

[2]Robinson J P, Harris S A, Juniper B E. Taxonomy of the genus Malus Mill. (Rosaceae) with emphasis on the cultivated apple, Malus domestica Borkh. Plant System Evolution, 2001, 226: 35-58.

[3]Harris S A, Robinson J P, Juniper B E. Genetic clues to the origin of the apple. Trends in Genetics, 2002, 18(8): 426-430.

[4]张新时. 伊犁野果林的生态地理特征和群落学问题.植物学报,1973,15(2):239-253.

Zhang X S. On the eco2geographical characters and the p roblems of classification of the wild fruit2tree forest in the Ili valley of Sinkiang. Acta Botanica Sinica, 1973, 15(2):239-253. ( in Chinese)

[5]Takos A M, Jaffe’ F W, Jacob S R, Bogs J, Robinson S P, Walker A R. Light-induced expression of a MYB gene regulates anthocyanin biosynthesis in red apples. Plant Physiology, 2006, 142(3): 1216-1232.

[6]Ban Y, Honda C, Hatsuyama Y, Igarashi M, Bessho H, Moriguchi T. Isolation and functional analysis of a MYB transcription factor gene that is a key regulator for the development of red coloration in apple skin. Plant and Cell Physiology, 2007, 48: 958-970.

[7]Espley R V, Hellens R P, Putterill J, Stevenson D E, Kutty-Amma S, Allan A C. Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10. The Plant Journal, 2007, 49: 414-427.

[8]Espley R V, Brendolise C, Chagné D, Kutty-Amma S, Green S, Volz R, Putterill J, Schouten H J, Gardiner S E, Hellens R P, Allan A C. Multiple repeats of a promoter segment causes transcription factor autoregulation in red apples. The Plant Cell, 2009, 21: 168-183.

[9]Ubi B. E. The genetics of anthocyanin reddening in apple skin. Food Agriculture & Environment, 2004, 2(1): 163-165.

[10]Ubi B E, Honda C, Bessho H, Kondo S, Wada M, Kobayashi S, Moriguchi T. Expression analysis of anthocyanin biosynthetic genes in apple skin: Effect of UV-B and temperature. Plant Science, 2006, 170: 571-578.

[11]Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 2001, 4: 447-456.

[12]Lancaster J E. Regulation of skin color in apples. Critical Reviews Plant Science, 1992, 10: 487-502.

[13]Honda C, Kotoda N, Wada S, Kondo S, Kobayashi S. anthocyanin biosynthesis genes are coordinately expressed during red coloration in apple skin. Plant Phsiology and Biochemistry, 2002, 40(11): 955-962.

[14]崔  彦, 束怀瑞.红色苹果花青苷的组成. 山东农业大学学报， 2000, 31(3) : 309-310.

Cui Y, Shu H R.. The kinds of anthocyanines in red apple. Journal of Shandong Agricultural University, 2000, 31(3): 309-310. (in Chinese)

[15]Kim S H, Lee J R, Hong S T, Yoo Y K, An G , Kim S R. Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin. Plant Science, 2003, 165:403-413.

[16]Chen X S, Feng T, Zhang Y M, He T M, Feng J R, Zhang C Y. Genetic diversity of volatile components in Xinjiang wild apple (Malus sieversii). Journal of Genetic and Genomics, 2007, 34(2): 171-179.

[17]张小燕, 陈学森, 彭 勇, 王海波, 石 俊, 张 红. 新疆野苹果   矿质元素与糖酸组分的遗传多样性, 园艺学报, 2008, 35(2): 277-280.

Zhang X Y, Chen X S, Peng Y, Wang H B, Shi J, Zhang H. Genetic diversity of mineral elements, sugar and acid components in Malus sieversii. Acta Horticulturae Sinica, 2008, 35(2): 277-280. (in Chinese)

[18]Zhang C Y, Chen X S, He T M, Liu X L, Feng T, Yuan Z H. Genetic diversity in Malus sieversii (Lebed. ) Ro2 em. Ⅱ. Population genetic structure in Malus sieversii revealed by SSR markers in Xinjiang, China. Journal of Genetic and Genomics, 2007, 34(10): 947-955

[19]张春雨, 陈学森, 林 群, 苑兆和, 张 红, 张小燕, 刘崇祺, 吴传 金. 新疆野苹果群体遗传结构和遗传多样性的SRAP 分析. 园艺学报, 2009, 36(1): 7-14.

Zhang C Y, Chen X S, Lin Q, Yuan Z H, Zhang H, Zhang X Y, Liu C Q, Wu C J. SRAP markers for population genetic structure and genetic diversity in Malus sieversii from Xinjiang, China. Acta Horticulturae Sinica, 2009, 36(1):7-14. ( in Chinese)

[20]张春雨, 陈学森, 张艳敏, 苑兆和, 刘遵春, 王延玲, 林 群. 采用分子标记构建新疆野苹果核心种质的方法. 中国农业科学, 2009, 42(2): 597-604.

Zhang C Y, Chen X S, Zhang Y M, Yuan Z H, Liu Z C, Wang Y L, Lin Q. A method for constructing core collection of Malus sieversii using molecular markers. Scientia Agricultura Sinica, 2009, 42(2):597-604. ( in Chinese)

[21]王延玲, 张艳敏, 冯守千, 田长平, 王海波, 刘遵春, 宋  杨, 陈学森. 新疆红肉苹果转录因子MsMYB10 基因的克隆、序列分析及原核表达. 中国农业科学, 2010, 43(13): 2735-2743.

Wang Y L, Zhang Y M, Feng S Q, Tian C P, Wang H B, Liu Z C, Song Y, Chen X S. Cloning, sequence analysis and expression in E.coli of MsMYB10 gene from Malus sieversii f. neidzwetzkyana. Scientia Agricultura Sinica, 2010, 43(13): 2735-2743. (in Chinese)

[22]冯守千, 陈学森, 张春雨, 刘晓静, 刘遵春, 王海波, 王延玲, 周朝华.砂梨品种‘满天红’及其芽变品系‘奥冠’花青苷合成与相关酶活性研究. 中国农业科学, 2008,41(10):3184-3190.

Feng S Q, Chen X S, Zhang C Y, Liu X J, Liu Z C, Wang Y L, Zhou C H.  Studys on the relationship between anthocyanin biosynthesis and related enzymes activity in Pyrus pyrifolia Mantianhong and its bud sports Aoguan. Scientia Agricultura Sinica, 2008, 41(10): 3184-3190. (in Chinese)

[23]Du Q Z, Jer Z G, Winterhalter P. Isolation of two anthocyanin sambubiosides from bilberry (Vaccinium myrtillus) by high-speed countercurrent chromatography. Journal of Chromatography A, 2004, 1045: 59-63.

[24]Allan A C, Hellens R P, Laing W A. MYB transcription factors that colour our fruit. Trends Plant Science, 2008, 13:99-102.

[25]Feng S Q, Wang Y L, Song Y, Xu Y T, Chen X S. Anthocyanin biosynthesis in pears is regulated by a R2R3-MYB transcription factor PyMYB10. Planta, 2010, 232(1): 245-255.

[26]Neuhaus G, Bowler C, Hiratsuka K, Yamagata H, Chua N H. Phytochrome-regulated repression of gene expression requires calcium and cGMP. The EMBO Journal, 1997, 16 (10): 2554-2564.
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