Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2295-2305.doi: 10.3864/j.issn.0578-1752.2012.11.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Gene Cloning and Distribution of Neuropeptide W and Its Receptor 1 in Rabbit

 SUN  Xiao-Xuan, LEI  Zhi-Hai, YANG  Gui-Hong, LIU  Xin-Yi, YE  Ping   

  1. 南京农业大学动物医学院，南京 210095
  • Received:2012-02-01 Online:2012-06-01 Published:2012-04-24

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Abstract: 【Objective】 This study was carried out to obtain sequence of NPW and NPWR1 (GPR7) gene, and reveal the expression of NPW and NPWR1 mRNA in rabbit organs. 【Method】 The partial coding sequences of NPW and NPWR1 were obtained from rabbit brain using the approach of gene cloning. The expressions of NPW and NPWR1 mRNA in rabbit brain were studied by semi-quantitative RT-PCR and in situ hybridization. 【Result】 The length of NPW and NPWR1 was 234 bp and 508 bp, respectively. The sequences of the products were confirmed to be those of NPW and NPWR1 in rabbit and enrolled in GenBank (accession numbers: HQ596517 and HQ596518). Phylogenetic analysis showed that amino sequence of rabbit NPS precursor had high homology compared with other animals. The expression of NPW and NPWR1 mRNA was extensive in rabbit organs, with majority of NPWR1 mRNA in the hippocampus, cerebellum, hypothalamus and medulla oblongata in the central nervous system. 【Conclusion】 NPW and its receptor gene in rabbit were conservative, and the expression of NPW and NPWR1 mRNA in rabbit organs were extensive, suggesting that they are involved in regulations of various physiological functions.

Key words: neuropeptide W, neuropeptide W receptor, gene cloning, distribution, rabbit

[1]雷治海, 苏  娟, 贾晓庆, 刘延鹏. 神经肽W和神经肽B的研究进展. 畜牧与兽医, 2010, 42(8): 94-99.

Lei Z H, Su J, Gia X Q, Liu Y P. The research on neuropeptide W and neuropeptide B. Animal Husbandry & Veterinary Medicine, 2010, 42 (8) :94-99. (in Chinese)

[2]Shimomura Y, Harada M, Goto M, Sugo T, Matsumoto Y, Abe M, Watanabe T, Asami T, Kitada C, Mori M, Onda H, Fujino M. Identification of neuropeptide W as the endogenous ligand for orphan G-protein-coupled receptors GPR7 and GPR8. The Journal of Biological Chemistry, 2002, 277:35826-35832.

[3]Fujii R, Yoshida H, Fukusumi S, Habata Y, Hosoya Y, Hosoya M, Kawamata Y, Yano T, Hinuma S, Kitada C, Asami T, Mori M, Fujisawa Y, Fujino M. Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7. The Journal of Biological Chemistry, 2002, 277:34010-34016.

[4]Brezillon S, Lannoy V, Franssen J D, Poul E L, Dupriez V, Lucchetti J, Detheux M, Parmentier M. Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8. The Journal of Biological Chemistry, 2003, 278:776-783.

[5]Tanaka H, Yoshida T, Miyamoto N, Motoike T, Kurosu H, Shibata K, Yamanaka A, Williams S C, Richardson J A, Tsujino N, Garry M G, Lerner M R, King D S, O’Dowd B F, S akurai T, Yanagisawa M. Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. Proceedings of the National Academy of Sciences, 2003, 100:6251-6256.

[6]O’Dowd B F, Scheideler M A, Nguyen T, Cheng R, Rasmussen J S, Marchese A, Zastawny R, Heng H Q, Tsui L C, Shi X M, Asa S, Puy L, George S R. The cloning and chromosomal mapping of two novel human opioid-somatostatin-like receptor genes. GPR7 and GPR8, expressed in discrete areas of the brain. Genomics, 1995, 28:84-91.

[7]Dun S L, Brailoiu G C, Yang J, Chang J K, Dun N J. Neuropeptide W-immunoreactivity in the hypothalamus and pituitary of the rat. Neuroscience Letters, 2003, 349(2): 71-74.

[8]Takenoya F, Yagi M, Kageyama H, Shiba K, Endo K, Nonaka N, Date Y, Nakazato M, Shioda S. Distribution of neuropeptide W in the rat    brain. Neuropeptides, 2010, 44:99-106.

[9]Jackson V R, Lin S H, Wang Z W, Nothacker H P, Civelli O. A study of the rat neuropeptide B/Neuropeptide W system using in situ techniques. The Journal of Comparative Neurology, 2006, 497: 367-383.

[10]Singh G, Maguire J J, Kuc R E, Fidock M, Davenport A P. Identification and cellular localisation of NPW1 (GPR7) receptors for the novel neuropeptide W-23 by [125I]- NPW radioligand binding and immunocytochemistry. Brain Research, 2004, 1017: 222-226.

[11]Hondo M, Ishii M, Sakurai. The NPB/NPW neuropeptide system and its role in regulating energy homeostasis, pain, and emotion. Results & Problems in Cell Differentiation, 2008, 46:239-256.

[12]Mondal M S, Yamaguchi H, Date Y, Shimbara T, Toshinai K, Shimomura Y, Mori M, Nakazato M. A Role for neuropeptide W in the regulation of feeding behavior. Endocrinology, 2003, 144(11): 4729-4733.

[13]Yamamoto T, Saito O, Shono K, Tanabe S. Anti-hyperalgesic effects of intrathecally administered neuropeptide W-23, and neuropeptide B, in tests of inflammatory pain in rats. Brain Resreach, 2005, 1045:97-106.

[14]Samson W K, Baker J R, Samson C K, Samson H W, Taylor M M. Central neuropeptide B administration activates stress hormone secretion and stimulates feeding in male rats. Journal of Neuroendocrinology, 2004, 16:842-849.

[15]Taylor M M, Yuill E A, Baker J R, Ferri C C, Ferguson A V, Samson W K. Actions of neuropeptide W in paraventricular hypothalamus: implications for the control of stress hormone secretion. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005, 288:R270-R275.

[16]Hochol A, Belloni A S, Rucinski M, Ziolkowska A, Di Liddo R, Nussdorfer G G, Malendowicz L K. Expression of neuropeptides B and W and their receptors in endocrine glands of the rat. Intenational Journal of Molecular Medicine, 2006, 18:1101-1106.

[17]Price C J, Samson W K, Ferguson A V. Neuropeptide W has cell phenotype-specific effects on the excitability of different subpopulations of paraventricular nucleus neurones. Journal of Neuroendocrinology, 2009, 21: 850-857.

[18]Taylor M M, Yuill E A, Baker J R, Ferri C C, Ferguson A V, Samson W K. Actions of neuropeptide W in paraventricular hypothalamus: implications for the control of stress hormone secretion. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 2005, 288: 270-275.

[19]Niimi M, Murao K. Neuropetide W as a stress mediator in the hypothalamus. Endocrine, 2005, 27(1): 51-54.

[20]Date Y, Mondal M S, Kageyama H, Ghamari-Langroudi M, Takenoya F, Yamaguchi H, Shimomura Y, Mori M, Murakami N, Shioda S, Cone R D, Nakazato M. Neuropeptide W: An anorectic peptide regulated by leptin and metabolic state. Endocrinology, 2010, 151(5):2200-2210.

[21]Kitamura Y, Tanaka H, Motoike T, Ishii M, Williams S C, Yanagisawa M, Takeshi S. Distribution of neuropeptide W immunoreactivity and mRNA in adult rat brain. Brain Research, 2006, 1093:123-134.

[22]Urban I, Richard P. A Stereotaxic Atlas of the New Zealand Rabbit’s Brain. Springfield. Illinois: Charles C Thomas Publisher, LTD. 1972.

[23]Berman A L. The Brain Stem of the Cat: A Cytoarchitectonic Atlas with Stereo Taxic Coordinates. London: The University of Chicago Press, 1968.

[24]Singh G, Davenport A P. Neuropeptide B and W: neurotransmitters in an emerging G-protein-coupled receptor system. British Journal of Pharmacology, 2006, 148: 1033-1041.

[25]Takenoya F, Kitamura S, Kageyama H, Nonaka N, Seki M, Itabashi K, Date Y, Masamitsu N, Shioda S. Neuronal interactions between neuropeptide W- and orexin- or melanin-concentrating hormone- containing neurons in the rat hypothalamus. Regulatory Peptides, 2008, 145:159-164.

[26]Zaratin P F, Quattrini A, Previtali S C, Comi G, Hervieu G, Scheideler M A. Schwann cell overexpression of the GPR7 receptor in inflammatory and painful neuropathies. Molecular and Cellular Neurosciences, 2005, 28:55-63.

[27]Takenoya F, Kageyama H, Shiba K, Date Y, Nakazato M, Shioda S. Neuropeptide W: a key player in the homeostatic regulation of feeding and energy metabolism? Animals of The New York Academy of Science, 2010, 1200:162-169.
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