Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2306-2314.doi: 10.3864/j.issn.0578-1752.2012.11.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Association Analysis of Three SNPs Within ANGPTL6 Gene with Growth Traits in Luxi Cattle

 LI  Ai-Min, MA  Yun, YANG  Dong-Ying, 蓝Xian-Yong , HU  Shen-Rong, LEI  Chu-Chao, ZHANG  Chun-Lei, CHEN  Hong   

  1. 1.西北农林科技大学动物科技学院，陕西杨凌 712100
    2.信阳师范学院生命科学学院，河南信阳 464000
    3.德州学院生物系，山东德州 253023
    4.江苏师范大学细胞与分子生物学研究所，江苏徐州 221116
  • Received:2011-12-31 Online:2012-06-01 Published:2012-04-24

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Abstract: 【Objective】 The purpose of this study is to reveal the genetic variation characteristics of bovine ANGPTL6 gene and find several candidate molecular markers that related to growth traits of Luxi cattle, thus for accumulating some basic information for molecular breeding of Luxi cattle breed.【Method】As investigative materials blood samples collected from 183 Luxi cows were used and sequence variants within ANGPTL6 gene were detected by DNA pool sequencing and PCR-RFLP technology.【Result】The results showed that three novel SNPs within the intron region of ANGPTL6 gene were identified in Luxi cattle breed. χ2 test showed that three loci of the Luxi cattle population were all in Hardy-Weinberg equilibrium (P＞0.05). Analysis of genetic diversity indicated T2359C and G3258T loci were at intermediate polymorphic status, but C2403A locus was at low polymorphic status. Further, the linkage disequilibrium and haplotypes were analyzed among three SNPs, suggested that they are all little linked. Interestingly, haplotype of TCG (wild-type) was dominant (with frequency of 44.3%). The variance analysis showed that the different genotypes of single and combined site were all significantly or extremely significantly associated with growth traits of Luxi cattle, and the growth traits of heterozygous individuals were all higher than the homozygous individuals.【Conclusion】The three novel SNPs are all significantly related to growth traits of Luxi cattle, and the heterozygous individuals are all more superior than the homozygous individuals, which suggested these results could apply to molecular breeding industry of Luxi cattle.

Key words: ANGPTL6 gene, polymorphism, association, growth trait, Luxi cattle

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