Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (11): 2295-2305.doi: 10.3864/j.issn.0578-1752.2012.11.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Gene Cloning and Distribution of Neuropeptide W and Its Receptor 1 in Rabbit

 SUN  Xiao-Xuan, LEI  Zhi-Hai, YANG  Gui-Hong, LIU  Xin-Yi, YE  Ping   

  1. 南京农业大学动物医学院，南京 210095
  • Received:2012-02-01 Online:2012-06-01 Published:2012-04-24

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Abstract: 【Objective】 This study was carried out to obtain sequence of NPW and NPWR1 (GPR7) gene, and reveal the expression of NPW and NPWR1 mRNA in rabbit organs. 【Method】 The partial coding sequences of NPW and NPWR1 were obtained from rabbit brain using the approach of gene cloning. The expressions of NPW and NPWR1 mRNA in rabbit brain were studied by semi-quantitative RT-PCR and in situ hybridization. 【Result】 The length of NPW and NPWR1 was 234 bp and 508 bp, respectively. The sequences of the products were confirmed to be those of NPW and NPWR1 in rabbit and enrolled in GenBank (accession numbers: HQ596517 and HQ596518). Phylogenetic analysis showed that amino sequence of rabbit NPS precursor had high homology compared with other animals. The expression of NPW and NPWR1 mRNA was extensive in rabbit organs, with majority of NPWR1 mRNA in the hippocampus, cerebellum, hypothalamus and medulla oblongata in the central nervous system. 【Conclusion】 NPW and its receptor gene in rabbit were conservative, and the expression of NPW and NPWR1 mRNA in rabbit organs were extensive, suggesting that they are involved in regulations of various physiological functions.

Key words: neuropeptide W, neuropeptide W receptor, gene cloning, distribution, rabbit

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