Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2040-2051.doi: 10.3864/j.issn.0578-1752.2012.10.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Genetic Diversity of Cynodon Dactylon Germplasm by SRAP and SSR Markers

 LING  Yao, ZHANG  Xin-Quan, CHEN  Shi-Yong, LIU  Wei, MA  Xiao   

  1. 1.四川农业大学动物医学院，四川雅安 652014
    2.四川农业大学草业科学系，四川雅安 625014
  • Received:2012-01-30 Online:2012-05-15 Published:2012-04-01

PDF

921

Cited

8

Abstract: 【Objective】 The aim of the study is to guide the introduction and utilization of germplasm resources, to provide a scientific basis for molecular marker-assisted breeding and to select parents for breeding programs accurately. 【Method】 In the study, SRAP and SSR markers were employed to investigate the genetic diversity and relationships of 52 wild accessions of Cynodon dactylon.【Result】 In a preliminary experiment, four accessions markedly distinct in phenotype were used to screen 150 pairs of SRAP primers and 200 pairs of SSR primers. Finally, 18 pairs of SRAP and SSR primers were selected and used for the present study. Eighteen primer pairs of SRAP and SSR produced a total of 236 and 346 bands, including 206 and 255 polymorphic bands, averaged 11.4 and 14.17 polymorphic bands per primer pair. The percentages of polymorphic bands were 87.29% and 73.70% in average. Combined the both markers with cluster analysis, when the GS is 0.68, the 52 accessions were classified into five major groups, GS is 0.78, the part V is divided into six groups. The accessions from the same origin frequently were clustered into one group. By the cluster analysis, the tested materials could be divided into eight eco-geographical groups. The Nei’s unbiased measures of genetic identity and genetic distance among eight eco-geographical groups showed the small genetic distance of geographic groups which have the similar eco-geographical environment. The data implied a correlation among the wild resources, geographical and ecological environment, which showed a significant relationship with the origined regions of accessions.【Conclusion】 The substantial genetic diversity was identified among the Cynodon dactylon germplasm. Furthermore, the results of cluster were related with the ecogeographical condition.

Key words: Cynodon dactylon, germplasm resource, SRAP marker, SSR marker, genetic diversity

[1]Taliaferro C M. Diversity and vulnerability of bermudagrass courfgrass species. Crop Abstracts, 1995, 35: 327-332.

[2]董宽虎, 沈益新. 饲草生产学. 北京: 中国农业出版社, 2003: 7.

Dong K H, Shen Y X. Forage Production Science. Beijing: China Agricultural Press, 2003: 7. (in Chinese)

[3]陈志一, 顾立新. 草坪草自然地带分类初探. 草业科学, 1999, 16(1): 54-59.

Chen Z Y, Gu L X. Prelminry probe to natural-zone classification of grass. Prata Cultural Science, 1999, 16(1): 54-59. (in Chinese)

[4]张小艾, 张新全, 杨春华, 刘  伟, 干友民. 狗牙根种质资源及遗传多样性的研究概况. 草原与草坪, 2003(4): 17-21.

Zhang X A, Zhang X Q, Yang C H, Liu W, Gang Y M. General conditions of bermudagrass germ resources and inheritance diversity. Grassland and Turf, 2003(4): 3-6. (in Chinese)

[5]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 2001, 103: 455-461.

[6]Budak H, Shearman R C, Parmaksiz I, Gaussoin R E, Riordan T P, Dweikat I. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theoretical and Applied Genetics, 2004, 108: 328-334.

[7]张安世, 徐九文, 辛长永, 张利民, 邢智峰, 韦慧彦. 河南省水稻中晚粳新品系遗传多样性的SRAP分析. 中国农学通报, 2010, 26(2): 50-54.

Zhang A S, Xu J W, Xin C Y, Zhang L M, Xing Z F, Wei H Y. Analysis of genetic diversity of new middle-late lines of Japonica rice in henan province by SRAP. Chinese Agricultural Science Bulletin, 2010, 26(2): 50-54. (in Chinese)

[8]姜树坤, 马  慧, 刘  君, 何  晶, 郭志富, 陈丽静, 钟  鸣, 张立军, 王学英, 张  丽. 利用SRAP标记分析玉米遗传多样性. 分子植物育种, 2007, 5(3): 412-416.

Jiang S K, Ma H, Liu J, He J, Guo Z F, Chen L J, Zhong M, Zhang L J, Wang X Y, Zhang L. Genetic diversity in maize inbred lines revealed by SRAPs marker. Molecular Plany Breeding, 2007, 5(3): 412-416. (in Chinese)

[9]何凤发, 杨志平, 张正圣, 王贵学, 王季春. 马铃薯遗传资源多样性的SRAP分析. 农业生物技术学报, 2007, 15(6): 1001-1005.

He F F, Yang Z P, Zhang Z S, Wang G X, Wang J C. Genetic diversity analysis of potato germplasm by SRAP makers. Journal of Agricultural Biotechnology, 2007, 15(6): 1001-1005. (in Chinese)

[10]郭海林, 刘建秀, 周志芳, 宣继萍. 结缕草属植物种间关系和遗传多样性的SSR标记分析. 草地学报, 2008, 16(6): 552-558.

Guo H L, Liu J X, Zhou Z F, Xuan J P. Interspecific relationship and genetic diversity of zoysiagrass revealed by SSR markers. Acta Agrestia Sinica, 2008, 16(6): 552-558. (in Chinese)

[11]丁成龙, 沈益新, 顾洪如, 三浦优一. 多花黑麦草遗传连锁图的构建. 草地学报, 2006, 14(3): 223-230.

Ding C L, Shen Y X, Gu H R, Sanpu Y Y. Construction of genetic linkage map in Lolium multiflorum. Acta Agrestia Sinica, 2006, 14(3): 223-230. (in Chinese)

[12]谢文刚, 张新全, 陈永霞. 鸭茅杂交种的SSR分子标记鉴定及其遗传变异分析. 草业学报, 2010, 19(2): 212-217.

Xie W G, Zhang X Q, Chen Y X. Identification and genetic variation analysis of orchardgrass hybrids (Dactylis glomerata) by SSR molecular markers. Acta Prataculturae Sinica, 2010, 19(2): 212-217. (in Chinese)

[13]Doyle J J. DNA protocols for plants-CTAB total DNA isolation. //Hewitt G M, Johnston A. Molecular Techniques in Taxonomy. Berlin: Springer-Ver1ag Press, 1991: 283-293.

[14]Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics, 2001, 103: 455-461.

[15]许绍斌, 陶  玉, 杨昭庆, 楮嘉祐. 简单快速的DNA银染和胶保存方法. 遗传, 2002, 24(3): 335-336.

Xu S B, Tao Y, Yang Z Q, Chu J Y. A simple and rapiad methods used for silver staining and gel preservation. Hereditas, 2002, 24(3): 335-336. (in Chinese)

[16]Nei M, Li W H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 1979, 76(10): 5269-5273.

[17]Nei M. Genetic distance between populations. American Naturalist, 1972, 106: 283-292.

[18]赵汝植. 西南区自然区划探讨. 西南师范大学学报: 自然科学版, 1997, 22(2): 193-198.

Zhao R Z. A study of the physico-geographical regionalization in Southwest region. Journal Of Southwest China Normal University: Natural Science, 1997, 22(2): 193-198. (in Chinese)

[19]Zabeau M, Vos P. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application No.92402629. Publication No.0−534−858−A, 1993.

[20]刘  伟. 西南区野生狗牙根种质资源遗传多样性与坪用价值研究[D]. 雅安: 四川农业大学, 2006.

Liu W. Genetic diversity of wild Cynodon dactylon germplasm in southwest of China and lawny value study[D]. Ya’an: Sichuan Agricultural University, 2006. (in Chinese)

[21]刘  伟, 张新全, 李  芳, 马  啸, 范  彦. 西南区野生狗牙根遗传多样性的ISSR标记与地理来源分析. 草业学报, 2007, 16(3): 55-61.

Liu W, Zhang X Q, Li F, Ma X, Fan Y. Genetic diversity of bermudagrass accessions in south-west China by ISSRs molecular markers and geographic provenance. Acta Pratacul Turae Sinica, 2007, 16(3): 55-61. (in Chinese)

[22]刘  伟, 张新全, 李  芳, 马  啸. 西南横断山区野生狗牙根遗传多样性的RAMP分析. 种子, 2008, 27(10): 56-58, 61.

Liu W, Zhang X Q, Li F, Ma X. Genetic diversity of Cynodon dactylon accessions based on RAMP markers in Hengduan Mountains from Southwest of China. Seed, 2008, 27(10): 56-58, 61. (in Chinese)

[23]易杨杰, 张新全, 黄琳凯, 凌  瑶, 马  啸, 刘  伟. 野生狗牙根种质遗传多样性的SRAP研究. 遗传, 2008, 30(1): 94-100.

Yi Y J, Zhang X Q, Huang L K, Ling Y, Ma X, Liu W. Genetic diversity of wild Cynodon dactylon germplasm detected by SRAP markers. Hereditas, 2008, 30(1): 94-100. (in Chinese)

[24]Wu Y Q, Taliaferro C M, Bai G H, Anderson M P. Genetic diversity of Cynodon transvaalensis Burtt-Davy and its relatedness to hexaploid C. dactylon (L.) Pers. as indicated by AFLP markers. Crop Science, 2005, 45: 848-853.

[25]Wu Y Q, Taliaferro C M, Bai G H, Martin D L, Anderson J A, Anderson M P, Edwards R M. Genetic analyses of Chinese Cynodon accessions by flow cytometry and AFLP markers. Crop Science, 2006, 46: 917-926.

[26]鄢家俊, 白史且, 张新全, 游明鸿, 张昌兵, 李达旭, 曾  怡. 青藏高原老芒麦种质基于SRAP标记的遗传多样性研究. 草业学报, 2010, 19(1): 173-183.

Yan J J, Bai S Q, Zhang X Q, You M H, Zhang C B, Li D X, Zeng Y. Genetic diversity of wild Elymus sibiricus germplasm from the Qinghai-Tibetan Plateau in China detected by SRAP markers. Acta Pratacul Turae Sinica, 2010, 19(1): 173-183. (in Chinese)

[27]陈永霞, 张新全, 马 啸, 谢文刚. SSR标记与扁穗牛鞭草农艺性状关联分析. 湖北农业科学, 2011, 50(7): 1494-1498.

Chen Y X, Zhang X Q, Ma X, Xie W G. Association analysis between SSR molecular markers and agronomic characters of hemarthria compressa. Hubei Agricural Sciences, 2011, 50(7): 1494-1498. (in Chinese)

[28]凌  瑶, 张新全, 齐晓芳, 周莹洁, 刘  伟, 马  啸, 陈仕勇. 西南五省区及非洲野生狗牙根种质基于SRAP标记的遗传多样性分析. 草业学报, 2010, 19(2): 196-203.

Ling Y, Zhang X Q, Qi X F, Zhou Y J, Liu W, Ma X, Chen S Y. Genetic diversity of wild Cynodon dactylon germplasm from five province of Southwest China and Africa detected by SRAP markers. Acta Pratacul Turae Sinica, 2010, 19(2): 196-203. (in Chinese)

[29]Hamarick J L, Godt M J W. Allozyme diversity in plant species. //Brown A H D, Clegg M T, Kahler A L, Weir B S. Plant Population Genetics, Breeding and Genetic Resources. Sunderland. M A: Sinauer Associates Press, 1989: 43-63.
[1] SONG SongQuan,LIU Jun,TANG CuiFang,CHENG HongYan,WANG WeiQing,ZHANG Qi,ZHANG WenHu,GAO JiaDong. Research Progress on the Physiology and Its Molecular Mechanism of Seed Desiccation Tolerance [J]. Scientia Agricultura Sinica, 2022, 55(6): 1047-1063.
[2] WANG XiuXiu,XING AiShuang,YANG Ru,HE ShouPu,JIA YinHua,PAN ZhaoE,WANG LiRu,DU XiongMing,SONG XianLiang. Comprehensive Evaluation of Phenotypic Characters of Nature Population in Upland Cotton [J]. Scientia Agricultura Sinica, 2022, 55(6): 1082-1094.
[3] JIANG Peng, ZHANG Peng, YAO JinBao, WU Lei, HE Yi, LI Chang, MA HongXiang, ZHANG Xu. Phenotypic Characteristics and Related Gene Analysis of Ningmai Series Wheat Varieties [J]. Scientia Agricultura Sinica, 2022, 55(2): 233-247.
[4] XiaoChuan LI,ChaoHai WANG,Ping ZHOU,Wei MA,Rui WU,ZhiHao SONG,Yan MEI. Deciphering of the Genetic Diversity After Field Late Blight Resistance Evaluation of Potato Breeds [J]. Scientia Agricultura Sinica, 2022, 55(18): 3484-3500.
[5] YingLing WAN,MengTing ZHU,AiQing LIU,YiJia JIN,Yan LIU. Phenotypic Diversity Analysis of Chinese Ornamental Herbaceous Peonies and Its Germplasm Resource Evaluation [J]. Scientia Agricultura Sinica, 2022, 55(18): 3629-3639.
[6] CHEN XueSen,WANG Nan,ZHANG ZongYing,MAO ZhiQuan,YIN ChengMiao. Understanding and Thinking About Some Problems of Fruit Tree Germplasm Resources and Genetic Breeding [J]. Scientia Agricultura Sinica, 2022, 55(17): 3395-3410.
[7] HU GuangMing,ZHANG Qiong,HAN Fei,LI DaWei,LI ZuoZhou,WANG Zhi,ZHAO TingTing,TIAN Hua,LIU XiaoLi,ZHONG CaiHong. Screening and Application of Universal SSR Molecular Marker Primers in Actinidia [J]. Scientia Agricultura Sinica, 2022, 55(17): 3411-3425.
[8] SHEN ZhiJun, TIAN Yu, CAI ZhiXiang, XU ZiYuan, YAN Juan, SUN Meng, MA RuiJuan, YU MingLiang. Evaluation of Brown Rot Resistance in Peach Based on Genetic Resources Conserved in National Germplasm Repository of Peach in Nanjing [J]. Scientia Agricultura Sinica, 2022, 55(15): 3018-3028.
[9] CHEN Xu,HAO YaQiong,NIE XingHua,YANG HaiYing,LIU Song,WANG XueFeng,CAO QingQin,QIN Ling,XING Yu. Association Analysis of Main Characteristics of Bur and Nut with SSR Markers in Chinese Chestnut [J]. Scientia Agricultura Sinica, 2022, 55(13): 2613-2628.
[10] XU Xiao,REN GenZeng,ZHAO XinRui,CHANG JinHua,CUI JiangHui. Accurate Identification and Comprehensive Evaluation of Panicle Phenotypic Traits of Landraces and Cultivars of Sorghum bicolor (L.) Moench in China [J]. Scientia Agricultura Sinica, 2022, 55(11): 2092-2108.
[11] TANG XiuJun,FAN YanFeng,JIA XiaoXu,GE QingLian,LU JunXian,TANG MengJun,HAN Wei,GAO YuShi. Genetic Diversity and Origin Characteristics of Chicken Species Based on Mitochondrial DNA D-loop Region [J]. Scientia Agricultura Sinica, 2021, 54(24): 5302-5315.
[12] LI XinYuan, LOU JinXiu, LIU QingYuan, HU Jian, ZHANG YingJun. Genetic Diversity Analysis of Rhizobia Associated with Medicago sativa Cultivated in Northeast and North China [J]. Scientia Agricultura Sinica, 2021, 54(16): 3393-3405.
[13] WANG FuQiang,ZHANG Jian,WEN ChangLong,FAN XiuCai,ZHANG Ying,SUN Lei,LIU ChongHuai,JIANG JianFu. Identification of Grape Cultivars Based on KASP Markers [J]. Scientia Agricultura Sinica, 2021, 54(13): 2830-2842.
[14] YANG Tao,HUANG YaJie,LI ShengMei,REN Dan,CUI JinXin,PANG Bo,YU Shuang,GAO WenWei. Genetic Diversity and Comprehensive Evaluation of Phenotypic Traits in Sea-Island Cotton Germplasm Resources [J]. Scientia Agricultura Sinica, 2021, 54(12): 2499-2509.
[15] SHEN ShengFa,XIANG Chao,WU LieHong,LI Bing,LUO ZhiGao. Analysis on the Characteristics of Soluble Sugar Components in Sweetpotato Storage Root and Its Relationship with Taste [J]. Scientia Agricultura Sinica, 2021, 54(1): 34-45.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd