Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2040-2051.doi: 10.3864/j.issn.0578-1752.2012.10.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Genetic Diversity of Cynodon Dactylon Germplasm by SRAP and SSR Markers

 LING  Yao, ZHANG  Xin-Quan, CHEN  Shi-Yong, LIU  Wei, MA  Xiao   

  1. 1.四川农业大学动物医学院，四川雅安 652014
    2.四川农业大学草业科学系，四川雅安 625014
  • Received:2012-01-30 Online:2012-05-15 Published:2012-04-01

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Abstract: 【Objective】 The aim of the study is to guide the introduction and utilization of germplasm resources, to provide a scientific basis for molecular marker-assisted breeding and to select parents for breeding programs accurately. 【Method】 In the study, SRAP and SSR markers were employed to investigate the genetic diversity and relationships of 52 wild accessions of Cynodon dactylon.【Result】 In a preliminary experiment, four accessions markedly distinct in phenotype were used to screen 150 pairs of SRAP primers and 200 pairs of SSR primers. Finally, 18 pairs of SRAP and SSR primers were selected and used for the present study. Eighteen primer pairs of SRAP and SSR produced a total of 236 and 346 bands, including 206 and 255 polymorphic bands, averaged 11.4 and 14.17 polymorphic bands per primer pair. The percentages of polymorphic bands were 87.29% and 73.70% in average. Combined the both markers with cluster analysis, when the GS is 0.68, the 52 accessions were classified into five major groups, GS is 0.78, the part V is divided into six groups. The accessions from the same origin frequently were clustered into one group. By the cluster analysis, the tested materials could be divided into eight eco-geographical groups. The Nei’s unbiased measures of genetic identity and genetic distance among eight eco-geographical groups showed the small genetic distance of geographic groups which have the similar eco-geographical environment. The data implied a correlation among the wild resources, geographical and ecological environment, which showed a significant relationship with the origined regions of accessions.【Conclusion】 The substantial genetic diversity was identified among the Cynodon dactylon germplasm. Furthermore, the results of cluster were related with the ecogeographical condition.

Key words: Cynodon dactylon, germplasm resource, SRAP marker, SSR marker, genetic diversity

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