褪黑激素对PDGFA基因在绒山羊皮肤毛囊中表达模式的影响

doi:10.3864/j.issn.0578-1752.2012.10.016

Abstract

Abstract: 【Objective】The expression regulation of PDGFA gene in skin follicles of Inner Mongolia Cashmere goat and the influence of melatonin on it were studied. 【Method】 Eight Cashmere mature femal goats with similar live weight were selected, and devided into four groups: never implanted as the control group and three groups of implanted melatonin in different times. The melatonin at a dose of 2 mg?kg-1 was implanted behind ears per 2 months during the implanting period. The body skin was collected in 12 months (from August 2009 to July 2010), and PDGFA gene expression was detected by using qRT-PCR. 【Result】The implanting MT into Cashmere goat influenced its hair growth cycle. The expression model of PDGFA gene in the skin and the hair growth cycle was different among groups. The lowest expression of PDGFA gene was noticed in November in control group. However, in December, the expression of PDGFA gene improved slowly with the hair growth went into catagen, and during telogen (from January to April), it kept higher level expression. After starting implanting MT into Cashmere goat, for the group just implanted melatonin in the second year, the expression of PDGFA gene was significantly improved (P<0.05) in next January. Similar results were found in implanted group for two years, it was lower with wool shedding in February, it was significantly lower in May than in January (P<0.05), and some new wool appeared on the skin at the same time. After stopping implanting MT for Cashmere goat, the PDGFA gene expression model of the group just implanted melatonin in the first year was similar with the control group.【Conclusion】 PDGFA gene had low expression in the Cashmere goat hair growth phase, and high expression in catagen and telogen. Melatonin implanted into Cashmere goat shortened hair growth cycle. PDGFA gene is a factor controlling the trarsformation of Cashmere goat hair from growth phase to catagen and telogen.

Key words: melatonin, platelet-derived growth factor A gene, skin hair follicle, hair growth circle

LI Chun, ZHANG Wen-Guang, YANG Wen-Zhu, WANG Zhi-Xin, ZHANG Yong-Bin, GAO Ai-Qin, ZHANG Yan-Jun, ZHAO Yan-Hong, SU Rui, CHANG Zi-Li, JIN Xin, WANG Peng-Yu, FENG Lin, LIU Hui, WU Rui-Bing, WANG Rui-Jun, LI Jin-Quan. Influence of Melatonin Implanting on PDGFA Gene Expression in Skin Hair Follicle[J].Scientia Agricultura Sinica, 2012, 45(10): 2031-2039.

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