Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2052-2057.doi: 10.3864/j.issn.0578-1752.2012.10.018

• VETERINARY SCIENCE • Previous Articles     Next Articles

Detection of PMQR Gene in Escherichia coli Isolated from Animals

 ZHUANG  Na, CHEN  Xue-Ying, YUE  Lei, LIAO  Xiao-Ping, LIU  Ya-Hong   

  1. 华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室，广州 510642
  • Received:2011-09-28 Online:2012-05-15 Published:2012-03-23

PDF

697

Cited

3

Abstract: 【Objective】The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance ( PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province.【Method】All the strains were screened for the oqxA, oqxB, qnr, qepA, aac (6′)-Ib-cr genes by PCR. The susceptibility of PMQR- positive strains to 18 antimicrobial agents were determined by agar dilution method.【Results】The positive rates of qnrB, qnrS, aac（6′）-Ib-cr，oqxA，oqxB genes were 10.49%, 18.88%, 31.47%, 44.8%, and 48.9% respectively. No qnrA, qnrC, qnrD and qepA genes were detected in any of the isolates. oqxA and oqxB have a high positive rate, and the two genes always exsist in the same isolates. PMQR gene always shows a coexstence of two or more kinds of the plasmid genes in the same isolates. PMQR positive isolates show a high rate of resistance to 18 antimicrobial agents which was widely used in veterinary clinical medicine. 【Conclusion】PMQR gene is very common in veterinary clinic. E. coli isolated from animals in Guangdong province shows a high level resistance to widely used drugs. The antibiotic sensitivity spectrum is variegated. The proportion of multi-drug-resistant strains is high.

Key words: oqxAB gene , Escherichia coli, plasmid mediate, quinolones, resistance gene

[1]Sørensen A H, Hansen L H, Johannesen E, Sørensen S J. Conjugative plasmid conferring resistance to olaquindox. Antimicrobial Agents and Chemotherapy, 2003, 47:798-799.

[2]Hansen L H, Johannesen E, Burmolle M, Sørensen A H, Sørensen S J. Plasmid-encoded multidrug efflux pump conferring resistance to olaquindox in Escherichia coli. Antimicrobial Agents and Chemotherapy, 2004, 48: 3332-3337.

[3]Norman A, Hansen L H, She Q X, Sørensena S J. Nucleotide sequence of  pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid, 2008, 60: 59-74.

[4]Hansen L H, Jensen L B, Sørensen H I, Sørensen S J. Substrate specificity of the oqxAB multidrug resistance pump in Escherichia coli and selected enteric bacteria. Antimicrobial Agents and Chemotherapy, 2007, 60:145–147.

[5]Kim H B, Wang M, Park C H, Kim E C, Jacoby G A, Hooper D C. oqxAB encoding a multidrug efflux pump in human clinical isolates of Enterobacteriaceae. Antimicrob. Agents Chemother, 2009, 53: 3582-3584.

[6]Strahilevitz J, JacobyG A, Hooper D C, Robicsek A. Plasmid- mediated quinolone resistance: a multifacete threat. Clinical Microbiology Reviews, 2009, 22:664-689.

[7]Martínez-Martínez L, Pascual A, Jacoby G A. Quinolone resistance

from a transferable plasmid. Lancet, 1998, 351: 797-799.

[8]Hata M, Suzuki M, Matsumoto M, Takahashi M, Sato K, Ibe S, Sakae

K. Cloning of a novel gene for quinolone resistance from a transferable  plasmid in Shigella flexneri 2b. Antimicrobial Agents and Chemotherapy, 2005, 49: 801-803.

[9]Jacoby G A, Walsh K E, Mills D M, Walker V J, Oh H, Robicsek A,

Hooper D C. qnrB, another plasmid-mediated gene for quinolone

resistance. Antimicrobial Agents and Chemotherapy, 2006, 50: 1178-1182.

[10]Robicsek A, Strahilevitz, Jacoby G A, Macielag M, Abbanat D, Park C H, Bush K, Hooper D C. Fluoroquinolone modifying enzyme: a novel adaptation of a common aminoglycoside acetyltransferase. Nature Medicine, 2006, 12: 83-88.

[11]Yamane K, Wachino J, Suzuki S, Kimura K, Shibata N, Kato H, Shibayama K, Konda T, Arakawa Y. New plasmid-mediated fluoroquinolone efflux Pump, QepA, found in an Escherichia coliclinical isolate. Antimicrobial Agents and Chemotherapy,  2007, 51:3354-3360.

[12]Zhao J, Chen Z, Chen S, Deng Y, Liu Y, Tian W, Huang X, Wu C, Sun Y, Sun Y, Zeng Z, Liu J H. Prevalence and Dissemination of oqxAB in Escherichia coli Isolates from Animals, Farmworkers, and the Environment. Antimicrobial Agents and Chemotherapy, 2010, 54(10): 4219- 4224.

[13]Cambau E, Lascols C, Sougakoff W, Bébéar C, Bonnet R, Cavallo J D, Gutmann L, Ploy M C, Jarlier V, Soussy C J, Robert J. Occurrence of qnrA-positive clinical isolates in French teaching hospitals during 2002-2005. Clinical Microbiology and Infection, 2006, 12(10): 1013-1020.

[14]Poirel L, Pitout J D D, Calvo L, Rodriguez-Martinez J M, Church D, Nordmann P. In vivo selection of fluoroquinolone-resistant Escherichia coli isolates expressing plasmid-mediated quinolone resistance and expanded-spectrum beta-lactamase. Antimicrobial Agents and Chemotherapy, 2006, 50(4): 1525-1527.

[15]Ma, J, Zeng, Z, Chen, Z, Xu, X, Wang, X, Deng, Y, Lü, D, Huang, L, Zhang, Y, Liu, J, Wang, M. High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6’)-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolatesceae isolates from companion and food-producing animals. Antimicrobial Agents and Chemotherapy, 2009, 53:519-524.

[16]杜向党, 连明香, 李德喜, 张素梅, 刘建华, 潘玉善, 李新生. 质粒介导的喹诺酮类耐药基因在猪源大肠杆菌中的检测. 江西农业学报, 2009, 21(8): 9-11.

Du X D, Lian M X, Li D X, Zhang S M, Liu J H, PianY S, Li  X  S.Detection of plasmid-mediated quinolone resistance among  Escherichia coli isolates from pigs. Jiangxi Journal of Agricultural Sciences, 2009, 21(8): 9-11.(in Chinese)

[17]Wang M, Tran J H, Jacoby G A, Zhang Y, Wang F, Hooper D C. Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai China. Antimicrobial Agents and Chemotherapy, 2003, 47: 2242-2248.

[18]赵 旭, 徐晓刚, 王明贵. 阴沟肠杆菌临床分离株中3类质粒介导喹诺酮类药物耐药基因检测. 中国感染与化疗杂志, 2009, 9(3): 210-215.

Zhao X, Xu X G, Wang M G. Detection of three kinds of plasmid-mediated quinolone resistance among Enterobacter cloacae. Chinese Journal of Infection and Chemotherapy, 2009, 9(3): 210-215. (in Chinese)

[19]Yue L, Jiang H X, Liao X P, Liu J H, Li S J, Chen X Y, Chen C X, Lu  D H, Liu Y H. Prevalence of plasmid-mediated quinolone resistance qnr genes in poultry and swine clinical isolates of Escherichia coli. Veterinary Microbiology, 2008, 132(3-4): 414-420.

[20]Wu J, Ko W, Tsai S, Yan J. Prevalence of plasmid-mediated quinolone resistance determinants QnrA, QnrB, and QnrS among clinical isolatesof Enterobacter cloacae in a Taiwanese hospital. Antimicrobial Agents and Chemotherapy, 2007, 49: 1223-1227.

[21]Jonas D, Biehler Y, Hartung D, Spitzmuller B, Daschner F D. Plasmid-mediated quinolone resistance in isolates obtained in German intensive care units. Antimicrobial Agents and Chemotherapy, 2005, 49(2):773-775.

[22]Poirel L, Rodriguez-Martinez J, Mammeri H, Liard A, Nordmann P.

Origin of plasmid-mediated quinolone resistance determinant QnrA.

Antimicrobial Agents and Chemotherapy, 2005, 49: 3523-3525.

[23]Kim H B, Park C H, Kim C J, Kim E C, JacobyG A, Hooper D C. Prevalence of plasmid-mediated quinolone resistance determinants over a 9-Year Period. Antimicrobial Agents and Chemotherapy, 2009, 53(2): 639-645.

[24]Wu C M, Wang Y, Cao X Y, Lin J C, Qin S S, Mi T J, Huang S Y, Shen J Z.. Emergence of plasmid-mediated quinolone resistance genes in Enterobacteriaceae isolated from chickens in China. Journal of Antimicrobial Chemotherogy, 2009, 63(2):409-411.

[25]Xiao Y H, Wang J, Li Y. Bacterial resistance surveillance in China: a report from Mohnarin 2004-2005. European Journal of Clinical Microbiology, 2008, 27:697-708.

[26]Yang H C, Chen S, White D G, Zhao S H, McDermott P, Walker R, Meng J H. Characterization of multiple- antimicrobial-resistant Escherichia coli isolates from diseased chickens and swine in China. Journal of Clinical Microbiology, 2004, 42:3483-3489.
[1] ZOU XiaoWei, XIA Lei, ZHU XiaoMin, SUN Hui, ZHOU Qi, QI Ji, ZHANG YaFeng, ZHENG Yan, JIANG ZhaoYuan. Analysis of Disease Resistance Induced by Ustilago maydis Strain with Overexpressed UM01240 Based on Transcriptome Sequencing [J]. Scientia Agricultura Sinica, 2025, 58(6): 1116-1130.
[2] XIE HuiHui, YANG QiuHua, LI WenLi, ZHU JinCheng, LI HuiXia, ZHANG Feng. Identification of Wild Potato Introgression Lines Resistant to Southern Root-Knot Nematode [J]. Scientia Agricultura Sinica, 2025, 58(14): 2924-2932.
[3] WU QiBin, XIE WanJie, ZHONG Hui, FENG ChunYan, PAN HaoRan, QI YiYing, ZHANG JiSen, WANG HengBo. Identification of the Bru1 Genomic Region for Brown Rust Resistance and Functional Analysis of Candidate Resistance Genes in Sugarcane [J]. Scientia Agricultura Sinica, 2024, 57(5): 855-867.
[4] GUI CuiLin, MA Liang, WANG YinYing, XIE FuGui, ZHAO CaiHong, WANG WenMiao, LI Xin, WANG Qing, GAO XiQuan. Identification of Resistant Germplasms and Mining of Candidate Genes Associated with Resistance to Stalk Rot Caused by Synergistic Infection with Fusarium spp. in Maize [J]. Scientia Agricultura Sinica, 2024, 57(13): 2509-2524.
[5] GAO Fu, WANG Rui, LIU DongJun, SUN HuiYan, WANG ZiYe, SONG WeiFu, LI TianYa. Stem Rust Resistance Genes Identification and Evaluation of 88 Wheat Cultivars (Lines) in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2024, 57(13): 2568-2582.
[6] ZHOU JingWei, YE BoWei, ZHANG PengFei, ZHANG YuQing, HAO Min, YIN YuRuo, YUAN Chan, LI ZhiKang, LI ShunDa, XIA XianChun, HE ZhongHu, ZHANG HongJun, LAN CaiXia. Identification and Evaluation of Stripe Rust Resistance in 153 Wheat Collections [J]. Scientia Agricultura Sinica, 2024, 57(1): 18-33.
[7] WANG JiangHao, WANG LiWei, ZHANG DongMin, GUO Rui, ZHANG QuanGuo, LI XingHua, WEI JianFeng, SONG Wei, WANG BaoQiang, LI RongGai. Molecular Marker Assisted Identification and Application of Maize Germplasms for Maize Rough Dwarf Disease Resistance [J]. Scientia Agricultura Sinica, 2023, 56(10): 1838-1847.
[8] LIU Jiao,LIU Chang,CHEN Jin,WANG MianZhi,XIONG WenGuang,ZENG ZhenLing. Distribution Characteristics of Prophage in Multidrug Resistant Escherichia coli as well as Its Induction and Isolation [J]. Scientia Agricultura Sinica, 2022, 55(7): 1469-1478.
[9] ZHANG YaLing, GAO Qing, ZHAO Yuhan, LIU Rui, FU Zhongju, LI Xue, SUN Yujia, JIN XueHui. Evaluation of Rice Blast Resistance and Genetic Structure Analysis of Rice Germplasm in Heilongjiang Province [J]. Scientia Agricultura Sinica, 2022, 55(4): 625-640.
[10] TANG ZiYun,HU JianXin,CHEN Jin,LU YiXing,KONG LingLi,DIAO Lu,ZHANG FaFu,XIONG WenGuang,ZENG ZhenLing. Relationship Between Biofilm Formation and Molecular Typing of Staphylococcus aureus from Animal Origin [J]. Scientia Agricultura Sinica, 2022, 55(3): 602-612.
[11] HU ChaoYue, WANG FengTao, LANG XiaoWei, FENG Jing, LI JunKai, LIN RuiMing, YAO XiaoBo. Resistance Analyses on Wheat Stripe Rust Resistance Genes to the Predominant Races of Puccinia striiformis f. sp. tritici in China [J]. Scientia Agricultura Sinica, 2022, 55(3): 491-502.
[12] WANG XueYang,JIANG JunYao,YANG Lu,SHAO DongYan,WU CongMing,SHEN JianZhong,SHEN YingBo,WANG Yang. Colistin Promotes mcr-1-positive IncI2 Plasmid Conjugation Between Escherichia coli [J]. Scientia Agricultura Sinica, 2022, 55(14): 2862-2874.
[13] CHEN ChaoXi,LI YuHan,TAN Min,WANG Lu,HUANG ZhiHong. Biofilm-Forming Phenotype, Antibacterial Resistance Genes, Integrase Genes and Virulence Genes Detection of Escherichia coli Isolated from Yaks and Tibetan Pigs in Northwest Sichuan Plateau [J]. Scientia Agricultura Sinica, 2021, 54(23): 5144-5162.
[14] LIU Jiao,CHEN ZhiMin,ZHENG AiJuan,LIU GuoHua,CAI HuiYi,CHANG WenHuan. Effects of Glucose Oxidase on Growth Performance, Immune Functions and Intestinal Health of Ducks Challenged by Escherichia coli [J]. Scientia Agricultura Sinica, 2021, 54(22): 4917-4930.
[15] Cheng LIU,Ran HAN,XiaoLu WANG,WenPing GONG,DunGong CHENG,XinYou CAO,AiFeng LIU,HaoSheng LI,JianJun LIU. Research Progress of Wheat Wild Hybridization, Disease Resistance Genes Transfer and Utilization [J]. Scientia Agricultura Sinica, 2020, 53(7): 1287-1308.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd