Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (10): 2052-2057.doi: 10.3864/j.issn.0578-1752.2012.10.018

• VETERINARY SCIENCE • Previous Articles     Next Articles

Detection of PMQR Gene in Escherichia coli Isolated from Animals

 ZHUANG  Na, CHEN  Xue-Ying, YUE  Lei, LIAO  Xiao-Ping, LIU  Ya-Hong   

  1. 华南农业大学兽医学院/广东省兽药研制与安全评价重点实验室，广州 510642
  • Received:2011-09-28 Online:2012-05-15 Published:2012-03-23

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Abstract: 【Objective】The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance ( PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province.【Method】All the strains were screened for the oqxA, oqxB, qnr, qepA, aac (6′)-Ib-cr genes by PCR. The susceptibility of PMQR- positive strains to 18 antimicrobial agents were determined by agar dilution method.【Results】The positive rates of qnrB, qnrS, aac（6′）-Ib-cr，oqxA，oqxB genes were 10.49%, 18.88%, 31.47%, 44.8%, and 48.9% respectively. No qnrA, qnrC, qnrD and qepA genes were detected in any of the isolates. oqxA and oqxB have a high positive rate, and the two genes always exsist in the same isolates. PMQR gene always shows a coexstence of two or more kinds of the plasmid genes in the same isolates. PMQR positive isolates show a high rate of resistance to 18 antimicrobial agents which was widely used in veterinary clinical medicine. 【Conclusion】PMQR gene is very common in veterinary clinic. E. coli isolated from animals in Guangdong province shows a high level resistance to widely used drugs. The antibiotic sensitivity spectrum is variegated. The proportion of multi-drug-resistant strains is high.

Key words: oqxAB gene , Escherichia coli, plasmid mediate, quinolones, resistance gene

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