Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (20): 4230-4239.doi: 10.3864/j.issn.0578-1752.2011.20.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Effects of Long-term Fertilization on Diversity of Ammonia-Oxidizing Archaea Communities and Abundance in Dry Highland Soil of Loess Plateau

 WU  Chuan-Dong, XIN  Liang, LI  Xiu-Ying, WANG  Bao-Li, QU  Dong   

  1. 1.西北农林科技大学生命科学学院
    2.西北农林科技大学资源环境学院
  • Received:2010-11-26 Online:2011-10-15 Published:2011-05-06

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Abstract: 【Objective】In order to improve the efficiency of nitrogen utilization and explicate the function of ammonia- oxidizing archaea (AOA) under the changes of soil quality in the Loess Plateau, the community structure diversity and abundance of AOA were studied. 【Method】 The influence of long-term fertilization treatments including CK, M, NM, PM and NPM on soil AOA community structure diversity and amoA gene copy numbers were analyzed by restriction fragment length polymorphism (PCR-RFLP) and real-time PCR.【Result】From the clone libraries of the different fertilization treatments, there were 25, 18, 29, 20 and 30 restriction endonuclease types, respectively. The α diversity indices indicated that there was a pronounced difference among five fertilizer treatments. The OTUs was the highest in NPM treatment and the lowest diversity in M treatment. The rescaled distance matrix tree indicated that the different fertilization had the largest convergence coefficient of AOA community types with the CK treatment soil, so the different fertilization led to significant changes of AOA communities. The amoA gene copy numbers of AOA changes were different among the treatments, whereas the highest copy numbers were detected in the NPM treatment, and had a pronounced difference with other fertilizer treatments. All preponderant sequences of AOA fell within soils/fresh water sediments based on phylogenetic tree of amoA gene amino acid sequences analysis.【Conclusion】Long-term fertilization resulted in changes of AOA community diversity and abundance.

Key words: long-termfertilization, ammonia-oxidizingarchaea, communitystructure, diversity

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